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Manipulation of Growth by UCulture Techniques

Sanette ThiartARC-Roodeplaat, Private Bag X293, Preto

Manipulation of Growth by Using Tissue Culture Techni

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ew cultivars can be selecte

rapidly dividing cells.Micropropagation can be usMicropropagation can causetics, e.g., bushiness.

roduction is independent oh

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or example, single-node cultures ar

oth apical and axillary buds are useddividing cells that can give rise to a new for the propagation of crops such as pot

Culture of Adventitious Tissue.from unusual points of origin. Shoot

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some species, like istacia , it is p

and root them directly ex vitro via convquicker and decreases costs in the labora

only used, and it does offer more contage IV, where the plantlets are trans

vironment, is very important, as this stag

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be used for unrooted cuttings. The poly

ing solution (Styroseal ) to prevent theecause ex vitro plantlets are so susc

sary to sterilise the growth medium useents must be supplied to the plantlets, ansolution (e.g., / MS) or by applying f

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Combined 66

are to be damaged. When using a seedl

are planted in the same container, the pla As soon as the leaves start to curl up, thdistilled water works very well.

Transplantation Time. The beswhen the temperature and humidity can

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yte, . 1987. Plants from test tubes. Anortland, Oregon.

urash ge, . 1974. Plant propagat o25:135-166.

orda n, . 1999. Conference: Mepropagat on. ISHS work ng groupvers ty College, Cork 24-27 Aug.,

Getting the Most From Your Lettuce Seedlings: Internati