threshold of compound action potential - mahidol · character of compound action potential and...
TRANSCRIPT
![Page 1: Threshold of compound action potential - Mahidol · Character of compound action potential and conduction velocity (1) stimulus stimulus artifact Latency (A) Action potential duration](https://reader031.vdocument.in/reader031/viewer/2022020316/5b3556597f8b9aec518d3026/html5/thumbnails/1.jpg)
1
thresholdpia.sfwdat : Page 1
durat:0.1ms A mpl:200mV
-4
-2
0
2
4
ampl
itude
(m
V) (
mV)
-4
-2
0
2
4
ampl
itude
(m
V) (
mV)
0 2 4 6 8 10 12ms (ms)
thresholdpia.sfw dat : Page 2
A mpl:220mV
-4
-2
0
2
4
ampl
itude
(m
V) (
mV)
-4
-2
0
2
4
ampl
itude
(m
V) (
mV)
0 2 4 6 8 10 12ms (ms)
thresholdpia.sfw dat : Page 3
A mpl:240
-4
-2
0
2
4
ampl
itude
(m
V) (
mV)
-4
-2
0
2
4
ampl
itude
(m
V) (
mV)
0 2 4 6 8 10 12ms (ms)
thresholdpia.sfwdat : Page 4
A mpl:260
-4
-2
0
2
4
ampl
itude
(m
V) (
mV)
-4
-2
0
2
4
ampl
itude
(m
V) (
mV)
0 2 4 6 8 10 12ms (ms)
Threshold of compound action potential
thresholdpia.sfwdat : Page 5
A mpl:280
-4
-2
0
2
4
ampl
itude
(m
V) (
mV)
-4
-2
0
2
4
ampl
itude
(m
V) (
mV)
0 2 4 6 8 10 12ms (ms)
thresholdpia.sfw dat : Page 6
A mpl:300
-4
-2
0
2
4
ampl
itude
(m
V) (
mV)
-4
-2
0
2
4
ampl
itude
(m
V) (
mV)
0 2 4 6 8 10 12ms (ms)
thresholdpia.sfw dat : Page 7
A mpl:320
-4
-2
0
2
4
ampl
itude
(m
V) (
mV)
-4
-2
0
2
4
ampl
itude
(m
V) (
mV)
0 2 4 6 8 10 12ms (ms)
thresholdpia.sfwdat : Page 8
A mpl:340
-4
-2
0
2
4
ampl
itude
(m
V) (
mV)
-4
-2
0
2
4
ampl
itude
(m
V) (
mV)
0 2 4 6 8 10 12ms (ms)
Threshold of compound action potential
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2
thresholdpia.sfwdat : Page 5
A mpl:280
-4
-2
0
2
4
ampl
itude
(m
V) (
mV)
-4
-2
0
2
4
ampl
itude
(m
V) (
mV)
0 2 4 6 8 10 12ms (ms)
thresholdpia.sfw dat : Page 6
A mpl:300
-4
-2
0
2
4
ampl
itude
(m
V) (
mV)
-4
-2
0
2
4
ampl
itude
(m
V) (
mV)
0 2 4 6 8 10 12ms (ms)
thresholdpia.sfw dat : Page 7
A mpl:320
-4
-2
0
2
4
ampl
itude
(m
V) (
mV)
-4
-2
0
2
4
ampl
itude
(m
V) (
mV)
0 2 4 6 8 10 12ms (ms)
thresholdpia.sfwdat : Page 8
A mpl:340
-4
-2
0
2
4
ampl
itude
(m
V) (
mV)
-4
-2
0
2
4
ampl
itude
(m
V) (
mV)
0 2 4 6 8 10 12ms (ms)
Threshold of compound action potential
Experiment: compound action potential
1 2 3 4 5 6 7 8
-+
PowerLab outputfor stimulation
To computer Dual BioAmp
1
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3
Akk Threshold.sfwdat : Page 18
190 mV
-4
-3
-2
-1
0
1
2
3
4
ampl
itude
(m
V) (
mV)
-4
-3
-2
-1
0
1
2
3
4
ampl
itude
(m
V) (
mV)
0 2 4 6 8 10 12ms (ms)
Akk Threshold.sfwdat : Page 19
200 mV
-4
-3
-2
-1
0
1
2
3
4
ampl
itude
(m
V) (
mV)
-4
-3
-2
-1
0
1
2
3
4
ampl
itude
(m
V) (
mV)
0 2 4 6 8 10 12ms (ms)
Akk Threshold.sfwdat : Page 20
210 mV
-4
-3
-2
-1
0
1
2
3
4
ampl
itude
(m
V) (
mV)
-4
-3
-2
-1
0
1
2
3
4
ampl
itude
(m
V) (
mV)
0 2 4 6 8 10 12ms (ms)
Akk Threshold.sfwdat : Page 21
220 mV
-4
-3
-2
-1
0
1
2
3
4
ampl
itude
(m
V) (
mV)
-4
-3
-2
-1
0
1
2
3
4
ampl
itude
(m
V) (
mV)
0 2 4 6 8 10 12ms (ms)
Threshold of compound action potentialเริ่มเห็น action potential
Thresholdstimulus
Stimulusmarker
Stimulusartifact
Noisy background
2
Akk Threshold.sfwdat : Page 22
230 mV
-4
-3
-2
-1
0
1
2
3
4
ampl
itude
(m
V) (
mV)
-4
-3
-2
-1
0
1
2
3
4
ampl
itude
(m
V) (
mV)
0 2 4 6 8 10 12ms (ms)
Akk Threshold.sfwdat : Page 23
240 mV
-4
-3
-2
-1
0
1
2
3
4
ampl
itude
(m
V) (
mV)
-4
-3
-2
-1
0
1
2
3
4
ampl
itude
(m
V) (
mV)
0 2 4 6 8 10 12ms (ms)
Akk Threshold.sfwdat : Page 24
250 mV
-4
-3
-2
-1
0
1
2
3
4
ampl
itude
(m
V) (
mV)
-4
-3
-2
-1
0
1
2
3
4
ampl
itude
(m
V) (
mV)
0 2 4 6 8 10 12ms (ms)
Akk Threshold.sfwdat : Page 25
260 mV
-4
-3
-2
-1
0
1
2
3
4
ampl
itude
(m
V) (
mV)
-4
-3
-2
-1
0
1
2
3
4
ampl
itude
(m
V) (
mV)
0 2 4 6 8 10 12ms (ms)
Threshold of compound action potential3
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4
Akk Threshold.sfwdat : Page 26
270 mV
-4
-3
-2
-1
0
1
2
3
4
ampl
itude
(m
V) (
mV)
-4
-3
-2
-1
0
1
2
3
4
ampl
itude
(m
V) (
mV)
0 2 4 6 8 10 12ms (ms)
Akk Threshold.sfwdat : Page 27
280 mV
-4
-3
-2
-1
0
1
2
3
4
ampl
itude
(m
V) (
mV)
-4
-3
-2
-1
0
1
2
3
4
ampl
itude
(m
V) (
mV)
0 2 4 6 8 10 12ms (ms)
Akk Threshold.sfwdat : Page 28
290 mV
-4
-3
-2
-1
0
1
2
3
4
ampl
itude
(m
V) (
mV)
-4
-3
-2
-1
0
1
2
3
4
ampl
itude
(m
V) (
mV)
0 2 4 6 8 10 12ms (ms)
Akk Threshold.sfwdat : Page 29
300 mV
-4
-3
-2
-1
0
1
2
3
4
ampl
itude
(m
V) (
mV)
-4
-3
-2
-1
0
1
2
3
4
ampl
itude
(m
V) (
mV)
0 2 4 6 8 10 12ms (ms)
Threshold of compound action potential4
Akk Threshold.sfwdat : Page 30
310 mV
-4
-3
-2
-1
0
1
2
3
4
ampl
itude
(m
V) (
mV)
-4
-3
-2
-1
0
1
2
3
4
ampl
itude
(m
V) (
mV)
0 2 4 6 8 10 12ms (ms)
Akk Threshold.sfwdat : Page 31
320 mV
-4
-3
-2
-1
0
1
2
3
4
ampl
itude
(m
V) (
mV)
-4
-3
-2
-1
0
1
2
3
4
ampl
itude
(m
V) (
mV)
0 2 4 6 8 10 12ms (ms)
Akk Threshold.sfwdat : Page 32
330 mV
-4
-3
-2
-1
0
1
2
3
4
ampl
itude
(m
V) (
mV)
-4
-3
-2
-1
0
1
2
3
4
ampl
itude
(m
V) (
mV)
0 2 4 6 8 10 12ms (ms)
Akk Threshold.sfwdat : Page 33
340 mV
-4
-3
-2
-1
0
1
2
3
4
ampl
itude
(m
V) (
mV)
-4
-3
-2
-1
0
1
2
3
4
ampl
itude
(m
V) (
mV)
0 2 4 6 8 10 12ms (ms)
Threshold of compound action potential5
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5
Akk Threshold.sfwdat : Page 34
350 mV
-4
-3
-2
-1
0
1
2
3
4
ampl
itude
(m
V) (
mV)
-4
-3
-2
-1
0
1
2
3
4
ampl
itude
(m
V) (
mV)
0 2 4 6 8 10 12ms (ms)
Akk Threshold.sfwdat : Page 35
360 mV
-4
-3
-2
-1
0
1
2
3
4
ampl
itude
(m
V) (
mV)
-4
-3
-2
-1
0
1
2
3
4
ampl
itude
(m
V) (
mV)
0 2 4 6 8 10 12ms (ms)
Akk Threshold.sfwdat : Page 36
370 mV
-4
-3
-2
-1
0
1
2
3
4
ampl
itude
(m
V) (
mV)
-4
-3
-2
-1
0
1
2
3
4
ampl
itude
(m
V) (
mV)
0 2 4 6 8 10 12ms (ms)
Akk Threshold.sfwdat : Page 37
380 mV
-4
-3
-2
-1
0
1
2
3
4
ampl
itude
(m
V) (
mV)
-4
-3
-2
-1
0
1
2
3
4
ampl
itude
(m
V) (
mV)
0 2 4 6 8 10 12ms (ms)
Threshold of compound action potential6
Akk Threshold.sfwdat : Page 38
390 mV
-4
-3
-2
-1
0
1
2
3
4
ampl
itude
(m
V) (
mV)
-4
-3
-2
-1
0
1
2
3
4
ampl
itude
(m
V) (
mV)
0 2 4 6 8 10 12ms (ms)
Akk Threshold.sfwdat : Page 39
400 mV
-4
-3
-2
-1
0
1
2
3
4
ampl
itude
(m
V) (
mV)
-4
-3
-2
-1
0
1
2
3
4
ampl
itude
(m
V) (
mV)
0 2 4 6 8 10 12ms (ms)
Akk Threshold.sfwdat : Page 40
410 mV
-4
-3
-2
-1
0
1
2
3
4
ampl
itude
(m
V) (
mV)
-4
-3
-2
-1
0
1
2
3
4
ampl
itude
(m
V) (
mV)
0 2 4 6 8 10 12ms (ms)
Akk Threshold.sfwdat : Page 41
420 mV
-4
-3
-2
-1
0
1
2
3
4
ampl
itude
(m
V) (
mV)
-4
-3
-2
-1
0
1
2
3
4
ampl
itude
(m
V) (
mV)
0 2 4 6 8 10 12ms (ms)
Threshold of compound action potential7
Maximal response
Maximalstimulus
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6
Akk Threshold.sfwdat : Page 41
430 mV
-4
-3
-2
-1
0
1
2
3
4
ampl
itude
(m
V) (
mV)
-4
-3
-2
-1
0
1
2
3
4
ampl
itude
(m
V) (
mV)
0 2 4 6 8 10 12ms (ms)
Character of compound action potentialand conduction velocity (1)
stimulus
stimulusartifact
Late
ncy
(A)
Act
ion
pote
ntia
l dur
atio
n
Late
ncy
(a)
8
ข้ัวบันทึกคูใกล (4-5)
ข้ัวบันทึกคูไกล (6-7)
Conduction velocity =Latency ไกล - Latency ใกล
ขั้วบันทึกแรกของคูไกล - ขั้วบันทึกแรกของคู ใกล
Conduction velocity =a - A
6 cm – 4 cm
Zoom of Akk Threshold.sfwdat : Page 41
430 mV
-4
-2
0
2
ampl
itude
(m
V) (
mV)
-4
-2
0
2
4
amplitude (m
V) (mV)
0 2 4 6 8 10 12ms (ms)
Peak action potential ที่ขั้วบันทึก 4
Peak action potential ที่ขั้วบันทึก 6
เวลาที่ action potential ใชในการเดนิทางจากขั้ว 4 ถึง 6 (t)
Conduction velocity =t
6 cm – 4 cm
Conduction velocity (2)9
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7
Biphasic action potential10
+- 0
+ -
+- 0
+ -
+ -
+- 0
Biphasic action potential11
+ -
+- 0
+ -
+- 0
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8
refracpia.sfwdat : Page 1
inter 7
-6
-4
-2
0
2
4
6
8
10
ampl
itude
(m
V) (
mV)
-8
-4
0
4
8
ampl
itude
(m
V) (
mV)
0 2 4 6 8 10 12ms (ms)
refracpia.sfwdat : Page 2
inter6.5
-6
-4
-2
0
2
4
6
8
10
ampl
itude
(m
V) (
mV)
-8
-4
0
4
8
ampl
itude
(m
V) (
mV)
0 2 4 6 8 10 12ms (ms)
refracpia.sfwdat : Page 13
inter1
-6
-4
-2
0
2
4
6
8
10
ampl
itude
(m
V) (
mV)
-8
-4
0
4
8
ampl
itude
(m
V) (
mV)
0 2 4 6 8 10 12ms (ms)
Refractory period
Stimulus interval 7 ms
Amplitude are the same
Stimulus interval 6.5 ms
Smaller 2nd action potential No 2nd action potential
Stimulus interval 1 ms
เร่ิมเขาสู Relative RP เร่ิมเขาสู Absolute RP
Total RP = 7 msAbsolute RP = 1 ms
12
Refractory period
Total RP
When stimulus interval is 1 ms
When stimulus interval is 7 ms
Absolute RP
Relative RP = Total RP-Absolute RP
Relative RP
13
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9
Anodal blockเมื่อกลับขั้วกระตุนโดยใชความแรงของตัวกระตุนเทาเดมิ
กอนกลับขั้วกระตุน หลังกลับขั้วกระตุน
• Amplitude ลดลง• Latency ยาวข้ึน• Stimulus artifact กลับหัว
กอน
หลัง
สังเกตุ: action potential ไมกลับหัว
14
Monophasic action potentialหลัง crush nerve ระหวางขั้วบันทึกที่ 4 และ 5
Biphasic action potential Monophasic action potential
15
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10
Zoom of block pia.sfwdat : Page 1
-1
0
1
2
3
4
5
6
ampl
itude
(m
V) (
mV)
0 2 4 6ms (ms)
Zoom of block pia.sfwdat : Page 2
-1
0
1
2
3
4
5
6
ampl
itude
(m
V) (
mV)
0 2 4 6ms (ms)
Zoom of block pia.sfwdat : Page 6
-1
0
1
2
3
4
5
6
ampl
itude
(m
V) (
mV)
0 2 4 6ms (ms)
Xylocaineหยดระหวางขั้วกระตุนและขั้วบันทึกที่ 4
กอนหยด xylocaine หลังหยด xylocaine
16
Exp3_Napaporn
.85 0.9 0.95 1 1.05 1.1 1.15 1.2 1.25 1.3 1.35 1.4 1.45 1.5 1.55 1.
7/6/2549 11:39:27.765
Character of skeletal muscle contraction
stimulus
Compound muscle action potential
Muscle contraction
17
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11
Threshold of muscle contraction(sciatic nerve stimulation)
400 mV 500 mVThresholdstimulus
600 mV 700 mV 800 mV 900 mVMaximalstimulus
1000 mV 1100 mV
Muscle contraction
Compound muscle action potential
Stimulus
18
L-T relationship -supornpim
Mus
cle
cont
ract
ion
(Ten
sion
) ...
0
20
40
60
1 2 3 4 5 6 7 8
EMG
(m
V)
-0.5
0.0
0.5
1.0
Mar
ker
(V)
-4
-2
0
2
4
5 0 0 0 0 0 0 5 03 4 5 6 7 8 9 10
6/6/2549 10:51:39.968 6/6/2549 10:52:09.359 6/6/2549 10:52:35.453 6/6/2549 10:53:50.562 6/6/2549 10:54:18.531 6/6/2549 10:54:34.671 6/6/2549 10:57:59.515 6/6/2549 10:58:23.906
Length-tension relationship
length +1 unit +2 unit +3 unit +4 unit +5 unit +6 unit +7 unit +8 unit
19
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12
Chart WindowM
uscl
e co
ntra
ctio
n (T
ensi
on)
(...
0
10
20
30
40
50
60
EMG
(µV
)
-1000
-500
0
500
Mar
ker
(V)
-4
-2
0
2
4
1 2 0 1 0 1 0 1 0 1 0 1 0
7/6/2549 11:10:47.546
Summation of muscle contraction
Stimulusinterval 600 ms 400 ms 200 ms 100 ms 80 ms 60 ms 40 ms
20
total lab
Mus
cle
cont
ract
ion
(Ten
sion
) ...
0
20
40
60
80
5 H
z
10 H
z
20 H
z
30 H
z
40 H
z
EMG
(m
V)
-4
-2
0
2
4
Mar
ker
(V)
-4
-2
0
2
4
2 4 0 2 4 0 2 4 0 2 4 0 2 446 47 48 49 50
8/6/2549 15:52:16.015 8/6/2549 15:52:52.984 8/6/2549 15:53:56.671 8/6/2549 15:54:49.421 8/6/2549 15:55:35.546
Tetanic contractionIncomplete tetanic contraction Complete tetanic contraction
21
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13
total lab
Mus
cle
cont
ract
ion
(Ten
sio.
..
0
20
40
60
80EM
G (
mV)
-4
-2
0
2
4
Mar
ker
(V)
-4
-2
0
2
4
1 1.5 2 2.5 3 3.5 4 4.5
8/6/2549 15:59:14.500
Muscle fatigue
Contraction = 1/3 of peak contraction
22
Exp4_Smooth Muscle_Akkradate
Chan
nel 1
(m
V)
-2
0
2
4
6
Ach,
3
7
ACh,
+3
35:00 36:40 38:20 40:00 41:40 43:20 45:00 46:40 48:20
16/6/2549 13:05:19.484
Smooth muscle contractionบทบาทของ Acetylcholine (ACh)
control ACh 3 drops Add Ach 3 drops
23
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14
E4-R301-Example
Chan
nel 2
(m
V)
-8
-6
-4
-2
0
2
40:00 40:50 41:40 42:30 43:20 44:10 45:50 46:40 47:30
15/6/2549 10:23:24.578 15/6/2549 11:08:37.078
Smooth muscle contractionบทบาทของ Adrenaline (E)
control E 3 drops Wash
24
E4-R301-Example
Chan
nel 1
(m
V)
-5
0
5
1:10:00 1:10:50 1:11:40 1:12:30 1:13:20 1:14:10 1:15:00 1:15:50 1:16:40 1:17:30 1:18:20 1:19:10
15/6/2549 11:08:37.078
control Cold ringer
Smooth muscle contractionบทบาทของอุณหภมูิ
25
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15
Exp.4 Smooth muscle 16-6-06
Chan
nel 1
(m
V)
-4
-2
0
2
4
16:40 17:30 18:20 19:10 20:50 21:40 22:30 23:20 24:10 25:00 25:50 26:40 27:30
16/6/2549 14:23:34.656 16/6/2549 14:43:44.656
control Ca2+ free Add Ca2+
Smooth muscle contractionบทบาทของ extracellular Ca2+
26
E4-R301-Example
Chan
nel 2
(m
V)
-8
-6
-4
-2
0
20
0.5%
KCl 3
dr
46:40 47:30 48:20 49:10 50:00 50:50 51:40 52:30
15/6/2549 11:08:37.078
control Add KCl
Smooth muscle contractionบทบาทของ extracellular K+
27
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16
E4-R301-Example
Chan
nel 2
(m
V)
-5
0
5
24
0.5%
BaCl
2 2
dr
55:50 56:40 57:30 58:20 59:10 1:00:00 1:00:50
15/6/2549 11:08:37.078
control Add BaCl2
Smooth muscle contractionบทบาทของ extracellular Ba2+
28