TILED DATA RECONSTRUCTION AND CORRECTION

ASHWINI NIKKAM JITHU JOSE SHRAVYA RAO

OUR GOALTiles must be fit or stitched

together to form one image. Automate the entire plugin.Minimum user interaction.Dynamic selection of the

directory of images.Accurately stitch multiple imagesCorrect the brightness

differences across the image

APPROACHObtain Image

Plus(.oib) Images

Conversion to Buffered Images

XML Parsing

Image Stitching

Conversion to Image Plus(.oib)

Image

DIRECTORY CHOOSERTwo directory choosers to

dynamically choose the path forStack of ImagesXML File

DIRECTORY CHOOSER

XML PARSINGXml file is parsed to obtain list of

nodes.For each node, values of Xno

and Yno are acquired using the tag name of the image.

Xno and Yno – position of images.

STITCHINGNumerous slices for each image.Similar indexes from each image

are stitched together.Gives multiple stitched images.Every stitched image is put into

stack to form final image.

STITCHINGImage stitching

Adds each image to the stack

Replaces the images with specified stack and updates display

RESULTS

REFERENCEShttp://fiji.sc/Bio-Formatshttp://stackoverflow.com/question

s/5997499/http://fiji.sc/Image_Stitchinghttp://java-forums.org http://rsb.info.nih.gov/ij/develope

r/api/ij/http://www.openmicroscopy.org/si

te/support/bio-formats5/developers/java-library.html

THANK YOU