tilv pathology and histopathology€¦ · nicholson and surachetpong et al., 2019 under review ......
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10/14/2019-10/17/2019 Training Course on EUS/TiLV Surveillance and Diagnostics
UTF/077/ZAM: Technical
Assistance to the Zambia
Aquaculture Enterprise
Development Project (ZAEDP)
TiLV Pathology and Histopathology
Kathy Tang-Nelson
Sample collection for diagnostic proceduresOrgans for TiLV diagnosis
Mucus
LiverSpleen
Head kidneyBrain
Meat
Training Course on EUS/TiLV Surveillance and Diagnostics10/14/2019-10/17/2019
310/14/2019-10/17/2019 Training Course on EUS/TiLV Surveillance and Diagnostics
Source: http://tupac.tue-image.nl/node/2
410/14/2019-10/17/2019 Training Course on EUS/TiLV Surveillance and Diagnostics
Normal
Infected
Gross pathology
Surachetpong et al., 2017 Emerging Infectious Diseases
510/14/2019-10/17/2019 Training Course on EUS/TiLV Surveillance and Diagnostics
Gross pathology
Nicholson and Surachetpong et al., 2019 Under review
• Ascites• Pale liver/gills• Corneal opacity
Brain show redness
Training Course on EUS/TiLV Surveillance and Diagnostics10/14/2019-10/17/2019
Training Course on EUS/TiLV Surveillance and Diagnostics10/14/2019-10/17/2019
810/14/2019-10/17/2019 Training Course on EUS/TiLV Surveillance and Diagnostics Thangaraj et al., 2018 Aquaculture
• Syncytial cells• Increase sinusoidal spaces
• Ascitic fluid, pale liver• Corneal opacity
910/14/2019-10/17/2019 Training Course on EUS/TiLV Surveillance and Diagnostics
Normal TiLV-infected
Win Surachetpong
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Normal TiLV challengedAnteriorkidney
Spleen
• Increase melanomacrophage
• Foamy cells
• Increase melanomacrophage
• Foamy cells, enlarge nucleus
• Loss of Red blood cells
• Accumulation of proteinacious
material
Tattiyapong et al., 2017 Vet Microbiology
Histopathology (level II)
11
Brain
Liver
Normal Infected
Training Course on EUS/TiLV Surveillance and Diagnostics10/14/2019-10/17/2019 Tattiyapong et al., 2017 Vet Microbiology
1210/14/2019-10/17/2019 Training Course on EUS/TiLV Surveillance and Diagnostics
Surachetpong et al., 2017 Emerging Infectious Diseases
Brain
Liver
1310/14/2019-10/17/2019 Training Course on EUS/TiLV Surveillance and Diagnostics Jansen et al., 2018 Rev Aqua
• Foamy cytoplasm
• Syncytial cells
• Intracytoplasmicinclusion body
1410/14/2019-10/17/2019 Training Course on EUS/TiLV Surveillance and Diagnostics Jansen et al., 2018 Rev Aqua
• Syncytial cells
• Severe infiltration of lymphocytes
• inflammation
H&E histology of eye lens in TiLV-infected tilapia. (A) Cataractous changes characterized by formation of
eosinophilic spherical structures accompanied by degeneration of crystalline fibers (encircled); (B) control
lens from healthy fish.
A B
Histopathological changes in eye
Training Course on EUS/TiLV Surveillance and Diagnostics10/14/2019-10/17/2019
Thank you for your attention
10/14/2019-10/17/2019 Training Course on EUS/TiLV Surveillance and Diagnostics
UTF/077/ZAM: Technical
Assistance to the Zambia
Aquaculture Enterprise
Development Project (ZAEDP)