Tissue homogenate with precipitation

Tissue homogenate without precipitation

Cy5: PP2A 0.1 U Cy5: PP2A 1U

Cy3: PP2A 0.1 U Cy3: PP2A 1U Cy3: PP2A 1U

Cy5: PP2A 1U

Supplementary Figure 2

pH 7pH 4

Cy5: PP1 1U

Cy3: PP1 1U Cy3: PP1 10U

Cy5: PP1 10U Cy5: PP1 1U

Cy3: PP1 1U

Tissue homogenate with precipitation

Tissue homogenate without precipitation

Supplementary Figure 3

pH 7pH 4

Cy5: AP 1U

Cy3: AP 1U Cy3: AP 10U

Cy5: AP 10U

Cy3: AP 10U

Cy5: AP 10U

Tissue homogenate with precipitation

Tissue homogenate without precipitation

Supplementary Figure 4

pH 7pH 4

Cy5: -PP 10U

Cy3: -PP 10U Cy3: -PP 100U

Cy5: -PP 100U Cy5: -PP 100U

Cy3: -PP 100U

Tissue homogenate with precipitation

Tissue homogenate without precipitation

Supplementary Figure 5

pH 7pH 4

Cy5: AP 10U , pH 9

Cy3: AP 10U , pH 9 Cy3: AP 10U, pH 9

Cy5: AP 10U, pH 9

Tissue homogenate with precipitation

Tissue homogenate without precipitation

Supplementary Figure 6

pH 7pH 4

Mitochondrial-enriched fraction resuspended in SDS/HEPES buffer (H-buffer)

Mitochondrial-enriched fraction resuspended in phosphatase buffer (P-buffer)

Cy3: AP 10U Cy3: AP 10U

Cy5: -PP 100U Cy5: AP 10U

Cy3: -PP 100U

Cy5: AP 10U

Supplementary Figure 7

pH 7pH 4

0.5U AP: Cy3 (green)

0.5U PP1: Cy5 (red)

0.5U PP2A: Cy2 (blue)

Supplementary Figure 8

pH 7pH 4