titration of abundance for purified gst-nups
DESCRIPTION
Titration of Abundance for Purified GST-Nups. 1 MW 2 -IPTG 3 +IPTG 4 MSS 5 blank 6 5 m L GST-Nup 7 50 m L GST-Nup 8 1 m g BSA 9 2 m g BSA 10 4 m g BSA. 1 MW 2 -IPTG 3 +IPTG 4 MSS 5 blank 6 5 m L GST-Nup 7 50 m L GST-Nup 8 1 m g BSA - PowerPoint PPT PresentationTRANSCRIPT
Titration of Abundance for Purified GST-Nups
A GST-Nsp1h (1-172) B GST-Nup116h (165-715) C GST-Nup100h (1-640)
D GST-Nup145h (1-242) E GST-Nup1h (798-1076) F GST-Nsp1 (377-471) K>R
G GST-Nsp1 (377-471) wild type H GST-Nup42 (1-382)
Titration of Abundance for Purified GST-Nups
Group GST-Nup Amino Acids Molecular Weight (daltons)
A GST-Nsp1h (1-172) 1-172 46,653
B GST-Nup116h (165-715) 165-715 83,844
C GST-Nup100h (1-640) 1-640 93,835
D GST-Nup145h (1-242) 1-242 53,498
E GST-Nup1h (798-1076) 798-1076 57,092
F GST-Nsp1 (377-471) K>R 371-471 39,841
G GST-Nsp1 (377-471) wild type 377-471 39,365
H GST-Nup42 (1-382) 1-382 67,144