to extract and compare caffeine and tannic acid content in different samples of tea.docx

8/9/2019 To extract and compare caffeine and tannic acid content in different samples of tea.docx

1/15

THIS IS TO CERTIFY THATISHMEET KAUR, A

BONAFIDE STUDENT OF CLASS Xll A HAS

COMPLETED THE CHEMISTRY ENTITLED TO

EXTRACT AND COMPARE CAFFIENE AND

TANNIC ACID CONTENT IN DIFFERENTSAMPLES OF TEA HERSELF AND UNDER MY

GUIDANCE. THE PROGRESS OF THE PROJECT

HAS BEEN CONTINOUSLY REPORTED AND HAS

BEEN IN MY KNOWLEDGE CONSISTENTLY.

CERT

IFI

CATE


2/15

I place ! "#$ce%e &'a$(" &) !

c'e#"&%! &eac'e% Ms.Amrit Kaur *)%

'e% +,#-a$ce a$- a-#ce" &) c)ple&e

! /)%( ",cce""*,ll!. I /),l- al") l#(e &)

&'a$( ! la0 #$c'a%+e Ms.Meesha *)%

'e% 'elp. T'#" p%)1ec& /),l- $)& 'ae

0ee$ *ea"#0le /#&'),& &'e p%)pe%

+,#-a$ce )* ! &eac'e%" /') +,#-e- e

&'%),+'),& &'e p%)1ec& . I al") &a(e &'#"

)pp)%&,$#&! &) place )$ %ec)%- ! -eep

+%a&,-e &) L)%- al#+'&! *)% &'e

c),$&le"" 0le""#$+ "')/e%e- )$ e

ACKN

OWLE

DGEMENT


3/15

/'#le -)#$+ &'e p%)1ec&. La"& 0,& $)& &'e

lea"&2 I /),l- l#(e &) &'a$( ! pa%e$&"

a$- *%#e$-" *)% &'e#% e$c),%a+ee$& a$-",pp)%& #$ ! ',0le e$&,%e.

T e!tra"t a#$ "m%are "a&ei#e a#$

ta##i" a"i$ "#te#t i# $i&ere#t sam%'es

( tea.

A

I

M


4/15

)ea*ers C#i"a' +as*s Fu##e' Heati#, arra#,eme#ts -arius sam%'e ( tea Test tue h'$er Measuri#, "/'i#$ers Disti''e$ 0ater SEPARATING FUNNEL

SPATUALA

WATCH GLASS

WEIGHING MACHINE

HEMI ALS USED :-

CALCIUM CAR)ONATE

CONC. HCL

SULPHURIC ACID

CAR)ON TETRACHLORIDE

APP

ARA

TUS

IN

T

RO

D

UC

T

I

O

N


5/15

Tea #" &'e )"& c))$l! a$- /#-el! ,"e- ")*&

0ee%a+e #$ &'e '),"e')l-. I& ac&" a" a ",la$& *)%

ce$&%al $e%)," "!"&e a$- "(ele&al ,"cle". T'a& #"

/'! &ea %e)e" *a+,e2 %e-$e"" a$- 'ea-ac'e. I&al") #$c%ea"e" &'e capac#&! )* &'#$(#$+. I& #" al") ,"e-

*)% l)/e%#$+ 0)-! &epe%a&,%e. T'e ace #$+%e-#e$&

&'a& a(e" &ea a$- c)3ee al,a0le &) ',a$" #"

ca3e#$e. Ca3e#$e #" a$ al(al)#-. Al(al)#-" a%e a cla"" )*

$a&,%all! )cc,%%#$+ c)p),$-" c)$&a#$#$+ $#&%)+e$ a$-

'a#$+ &'e p%)pe%e" )* a$ )%+a$#c a#$e 0a"e.

Ca3e#$e #" a $a&,%all! )cc,%%#$+ al(al)#- *),$- #$ )e%

45 pla$& "pec#e". Ca3e#$e 0el)$+" &) a *a#l! )*

$a&,%all! )cc,%%#$+ c)p),$-" ($)/$ a" 6a$&'#$e".

T'e 6a$&'#$e"2 /'#c' c)e *%) pla$&"2 a%e p)""#0l!

&'e )l-e"& ($)/$ ",la$&". Ca3e#$e #" &'e )"&

p)/e%*,l 6a$&'#$e #$ #&" a0#l#&! &) #$c%ea"e ale%&$e""2p,& )3 "leep a$- &) #$c%ea"e )$e" capac#&! *)% &'#$(#$+.

Ca3e#$e #" a a")-#la&)% 7%ela6e" &'e 0l))- e""el"8 a"

/ell a" a -#,%ec 7#$c%ea"e" ,%#$a)$8.

O%#+#$all! #& /a" &'),+'& &'a& ca3e#$e #" %e"p)$"#0le *)%

&'e &a"&e a$- 9a),% )* &ea. B,& p,%e ca3e#$e 'a" 0ee$*),$- &) 0e a &a"&ele"" /'#&e ",0"&a$ce. T'e%e*)%e2 &'e

&a"&e a$- 9a),% )* &ea #" -,e &) ")e )&'e% ",0"&a$ce

p%e"e$& #$ #&. Ca3e#$e -)e" $)& e6#"& al)$e #$ &ea

leae": &'e leae" a%e a#$l! cell,l)"e2 p#+e$&"2

p)l!p'e$)l#c c)p,$-" a$- c'l)%)p'!ll"2 a$- &a$$#$".

T'e 0%)/$#"' c)l),% )* &'e &ea #" -,e &) &'e p%e"e$ce )*


6/15

p)l!p'e$)l#c c)p),$-" a$- ")e #$)%+a$#c #)$" l#(e

a+$e"#, #)$ 2 *e%%#c #)$2 e&c..

S)e )* &'e 0e&&e%;($)/$ pla$& "),%ce" a%e c)3ee a$-

c)c)a 0ea$"2 &ealeae"2 a$- ()la $,&". W'#le c)3ee

a$- &ea a%e 0)&' p)p,la% p%)-,c&" c)$&a#$#$+ ca3e#$e2

&'e a),$&" a%! /#-el! #$ a "#$+le "e%#$+. T) *,%&'e%

c)$*,"e &'e a&&e%2 c)3ee 0ea$" c)$&a#$ le"" ca3e#$e

&'a$ &ea leae" /'e$ ea",%e- -%!. H)/ee%2 a

"e%#$+ )* c)3ee c)$&a#$" %),+'l! &/#ce &'e ca3e#$e )*&ea. M,c' )* &'e 9a)% )* c)3ee a$- &ea c)e" *%)

&a$$#$" a$- )&'e% 9a)%#$+ a+e$&". Ca3e#$e 'a" a

"l#+'&l! 0#&&e% 9a)%. A" a %e",l&2 -eca3e#$a$+ c)3ee

0ea$" a$- &ea leae" /#ll leae &'e 9a)% "l#+'&l!

c'a$+e- ee$ #* $) )&'e% c)p),$-" a%e l)"&.

FOLLOWING CHART SHOWS CAFFIENE CONTENT

IN DIFFERENT FOOD ITEMS 12

FOOD ITEM CAFFIENECONTENT

3UANTIT4


7/15

TEA MG

PER CUP

COFFEE ?5; @> MG

PER CUP

COFFEE ; MG

PER CUP

DECAFFEINATED

COCOA


8/15

Ca&ei#e is a #atura' %r$u"t e'#,i#, t the

a'*a'i$ "'ass. It 0as re%ute$ t ha5e 6rst ee#

"#sume$ / a Chi#ese em%err7 She# Nue,7 i#aut 89:: ).C7 ut it a"tua''/ has ee#

"#sume$ si#"e the st#e a,e. Ca&ei#e 0as

a"tua''/ 6rst $is"5ere$ a#$ is'ate$ / Germa#

"hemist Frie$ri"h Ru#,e. The stru"ture ( the

m'e"u'e 0as $is"5ere$ / Herma## Fis"her.

Si#"e its $is"5er/7 "a&ei#e has "ertai#'/ #t

$e"'i#e$ i# use7 ut it is a"*#0'e$,e$ / hea'th;"ia's as $a#,erus i# 'ar,e amu#ts.

This $ru, is (u#$ mai#'/ i# "&ee7 tea7 sme

s$as7 a#$ e#er,/ $ri#*s. It is a %s/"ha"ti5e

stimu'a#t $ru, i# huma#s a#$ ther a#ima's7

0here it 0ar$s ( $r0si#ess7 a#$ mai#tai#s

a'ert#ess i# the huma# $/. It is 5er/ "mm#'/(u#$ i# the ea#s7 'ea5es7 a#$ (ruit ( 5er 9:

$i&ere#t s%e"ies ( %'a#t7 a#$ as a resu't it is the

0r'$


9/15

e!am%'es are mr%hi#e7 heri#7 '/ser,i" a"i$

?LSD@7 ""ai#e7 ui#i#e7 str/"h#i#e7 a#$ #i"ti#e.

Ca&ei#eBs m'e"u'ar (rmu'a is CsH:NO8. Its

IUPAC #ame is 7 7 92trimeth/'!a#the#e a#$

"mm# #ame is 2meth/'ate$ thirmi#e.

Pure'/ it is 0hite7 "r/sta''i#e s'i$ i# the (rm (

#ee$'es. Its me'ti#, %i#t is 8:". It is the mai#

a"ti5e %ri#"i%'e "m%#e#t ( tea 'ea5es. It is

%rese#t i# tea 'ea5es u% t = a#$ "a# e

e!tra"te$ / 6rst i'i#, the tea 'ea5es 0ith

0ater 0hi"h $iss'5es ma#/ ,'/"si$e

"m%u#$s i# a$$iti# t "a&ei#e. The "'ear

s'uti# is the# treate$ 0ith 'ea$ a"etate t

%re"i%itate the ,'/"si$e "m%u#$s i# the (rm

( 'ea$ "m%'e!. The "'ear 6'trate is the#

e!tra"te$ 0ith e!tra"ts "a&ei#e e"ause it is

mre s'u'e i# it the# 0ater.

I$ &'#" %eac)$2 /e-eca3e#$a&e- &ea #$ )%-e% &) )0&a#$ &'e ca3e#$e. I$

&'#" e&')-2 /e ,"e- -#c'l)%)e&'a$e a" a ")le$& #$

)%-e% &) a0")%0 &'e ca3e#$e a$- "epa%a&e #& *%) &'e

/a&e% ") &'a& &'e ca3e#$e c),l- 0e e6&%ac&e- la&e%.

D#c'l)%)e&'a$e #" ,"e- 0eca,"e #& ,c' le""

-a$+e%)," &'a$ )&'e% ")le$&"2 a" #& -)e"$& ca,"e

0%a#$ )% %e"p#%a&)%! -aa+e.


10/15

STRUCTURES OF SOME

COMPOUNDS

TANNIC ACID

CAFFEINE MOLECULE


11/15

P%)ce-,%e

@. /EIGH @5 + OF EACH OF THE GIEN SAMPLES OF TEA

LEAES.. TAKE @55 ML OF DISTILLED WATER IN A 55 ML BEAKER

AND BOIL IT.THEN ADD THE FIRST SAMPLE OF TEA LEAES

TO IT AND BOIL FOR @5 MINUTES. COOL AND FILTER THE

SOLUTION.


12/15

PRECIPITATED AS CALCIUM TANNATE.SEPERATE THE

PRECIPITATE OF CALCIUM TANNATE BY FILTRATION.

7PRESERE THE FILTRATE FOR EXPERIMENT ML OF CONC. HCI. THE TANNIC ACIDOBTAINED IS CRYSTALLISED FROM WATER AND WEIGHED.

>. REPEAT THE STEPS 2< AND WITH THE OTHER SAMPLES

OF TEA AND RECORD THE OBSERATIONS.. TO THE FILTERATE FROM 7STEP


13/15

Ta'e (r ta##i" a"i$ "#te#t

".$). B%a$-

$ae )*

&'e &ea

We#+'& )*

&ea leae"

&a(e$ 6+%a"

We#+'& )*

&a$$#c ac#-

)0&a#$e- !+%a"

)* &a$$#c

ac#- !=6

@55

@.

.

Ta'e (r "a&ei#e "#te#t". $). B%a$-

$ae )*

&'e &ea

We#+'& )*

&ea leae"

&a(e$

+%a"

We#+'& )*

ca3e#$e

)0&a#$e-

p +%a"

)*

ca3e#$e

p=@55

O)ESER

-ATI

ONS


14/15


15/15

C'e#"&%!

THE TANNIC ACID PRESENT IN TEA LEAES IS PRECIPITATED AS

CALCIUM TANNATE BY TREATMENT OF AUEOUS SOLUTION OF

TEA WITH CALCIUM CARBONATE. CALCIUM TENNATE IS THENHYDROLYSED WITH CONC. HCI AND RECRYSTALLISED FROM

WATER.

TEA CONTAINS CAFFEINE UP TO . IT IS MORE SOLUBLE IN

CHLOROFORM THAN IN WATER. THEREFORE2 IT CAN BE

EXTRACTED FROM THE AUEOUS EXTRACT OF THE TEA WITH

CHOLROFOAM. HOWEER2 TANNIC ACID AND LEAD INTERFERE

IN THE EXTRACTION AND2 THEREFORE2 THESE HAE TO BE

REMOED FIRST.

TANNIC ACID IS REMOED AS CALCIUM TANNATE AND LEAD AS

LEAD SULPHATE. AFTER THE REMOAL OF TANNIC ACID AND

LEAD2 CAFFINE IS EXTRACTED WITH CHLOROFOAM.

to extract and compare caffeine and tannic acid content in different samples of tea.docx

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