today’s lecture summary: microscopes a tour of the cell
TRANSCRIPT
Today’s Lecture Summary:Today’s Lecture Summary:
MicroscopesMicroscopes
A Tour of the Cell A Tour of the Cell
Learning Outcomes:
By the end of today’s lecture, you should be able to:
• Recognize the different kinds of images seen through different microscopes
• Identify the major parts and functions of prokaryotic and eukaryotic cells
How we study cells:How we study cells:Light microscope (LM)Light microscope (LM)Dissecting MicroscopeDissecting MicroscopeTransmission electron microscope (TEM)Transmission electron microscope (TEM)Scanning electron microscope (SEM)Scanning electron microscope (SEM)
Magnification: - the ratio of an object’s image to its real size (e.g. 40X, 100X)
Resolving power: - the measure of the clarity of an image- the minimum distance two points can be separated and still be distinguished as being separate
Light/Compound Light/Compound Microscope:Microscope:
- uses light to magnify uses light to magnify an objectan object
- gives a 2-D imagegives a 2-D image
- the different images - the different images are based on the are based on the various staining various staining techniques usedtechniques used
Electron microscopes:Electron microscopes: specimen preparation kills the cellsspecimen preparation kills the cells have a higher resolution than LMhave a higher resolution than LMTEM:TEM: SEM:SEM:2-D images2-D images 3-D images3-D images
1 cm = 1/100m
1mm = 1/1000m
1μm = 1 micrometer = 1 millionth of a meter
1nm = 1 nanometer = 1 billionth of a meter
Light and electron microscopes have different magnification and resolving powers.
Learning Outcomes:
By the end of today’s lecture, you should be able to:
• Recognize the different kinds of images seen through different microscopes
• Identify the major parts and functions of prokaryotic and eukaryotic cells
Learning Outcomes:
By the end of today’s lecture, you should be able to:
• Recognize the different kinds of images seen through different microscopes
• Identify the major parts and functions of prokaryotic and eukaryotic cells