topic 8: dna exercise i: isolation analysis plasmid dna topic 7: cellular genetics. exercise ii...
TRANSCRIPT
Topic 8: DNA Exercise I: Isolation Analysis Plasmid DNA
Topic 7: Cellular Genetics.Exercise II Mitosis Cell Cycle
Exp: Cell cycle onion tips
Structure of Escherichia coli.
Two strains of E.coli: pBR322 and pUC18.
Purification Plasmid DNA 1:
Purification Plasmid DNA 1:
Spin and remove most contaminants:
Purification Plasmid DNA 2:
Addition of very small volumes:
DNAse and RNAse treatment:
Preparing the Agarose gel:
Agarose Gel.
Electrophoresis:
Electrophoresis:
Electrophoretic Mobility of Nucleic Acids:
pBR322 pUC18
ET D R ET D R
pUC18 pBR322 ET D R ET R D
MITOSIS: Onion root tip.
Onion root tip.
Onion root tip.