training curriculum and registration form - biot.doc
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8/10/2019 Training curriculum and Registration form - BIOT.doc
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Training Curriculum
Techniques in Molecular Biology
Theory and practical in
Macro molecules – DNA, RNA and protein - their properties and function – isolation from plants
and microbes. Restriction digestion. Spectrophotometry Agarose and Polyacrylamide gel
electrophoresis for nucleic acids. Protein purification. SDS page. lotting techni!ues. "estern
lot. Southern lot. Recombinant DNA technology – cloning #ectors and cloning. $igation,
transformation and recombinant selection. Polymerase chain reaction. Molecular mar%ers and
genome mapping. Analysis of data using soft&ares. Se!uence analysis using ioinformatics
tools. 'M technology and biosafety regulations.
Plant Tissue Culture and Applications
Theory and practical in
Plant tissue culture – principles and concepts – techni!ues in P() – essential re!uirements –
different media used – culture conditions – different routes – organogenesis, embryogenesis –
direct and indirect methods. Different stages – establishment, multiplication, proliferation, rooting
and hardening. Applications – mass multiplication, crop impro#ement, conser#ation, secondarymetabolite production, transgenics. *alidation methods – clonal fidelity, genetic stability. (rouble
shooting – concerns and issues. )ost benefit analaysis.
Hands on Training in techniques in Molecular Biology and Biotechnology
+ands on eperience &ill be pro#ided to #arious techni!ues li%e plant tissue culture, isolation of
nucleic acids, P)R techni!ues, agarose gel electrophoresis and gel documentation, primer
designing, purification of P)R products, preparation of competent cells, cloning, restriction
analysis, se!uencing and se!uence analysis, molecular mar%ers RAPD, SSR, SSR/, genetic
engineering, blotting and hybridi0ation, molecular diagnostics, protein etraction and analysis by
PA'1 and SDS page, chromatography techni!ues, 1$SA en0yme lin%ed immune sorbent
assay/, production of biofertilisers etc
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REGISTRATI! "RMCE!TRE "R BITECH!#G$ A!% M#EC&#AR BI#G$ 'CPBMB()
IT*BT CMP#E+) ,ERA#A AGRIC&#T&RA# &!I-ERSIT$
-E##A!I,,ARA .P/0) THRISS&R 1 234 252
Tel6 4738*97:35881-mail2 cpbmb%au3gmail.com, %aubioinfo3gmail.com, cpbmb3%au.in4. Name 2
5. Designation 2
6. Speciali0ation 2
7. Post graduation 8)ompleted9 pursuing: 2
;. nstitute9)ollege9<ni#ersity 2
=. (elephone No. 2
>. Address for )orrespondence 2
?. 1-mail Address 2
@. (raining programme applied for 2
Preference
4. (echni!ues in Molecular iology ..
5. Plant tissue culture B Applications ..
6. +ands on training on (echni!ues in Molecular iology and iotechnology .......
Mar% a tic% √/ legibly. Applicants can apply for more than one programme in a single registration form. n that
case preference may be indicated in Roman numeral against the Sl. No. of the programme. (he follo&ing
information may please be gi#en2-
4C. Mode of payment2 i/ n cash at )entral (raining nstitute or ii/ As DD in fa#our of Professor of
1tension, )(, Mannuthy payable at S(, llu%%ara and to be sent to2 Professor B +ead, )PM,
(-( )omple, Eerala Agricultural <ni#ersity, *ellani%%ara, 8P.:, (hrissur – =?C =;=
DD no.... Dated.. Amount..
44. Four in#ol#ement in Plant iotechnology B Molecular iology2
45. ProGect title f applicable/ 2
46. Details of similar training9&or%shop attended 2
47. Hustification for considering for the training Programme selected 2
Place2
Date2 Signature