transplastomic technology for safer and better transgenic crops
TRANSCRIPT
28 ❖ Speakers Agbiotech 99
Speakers •
Prof. Pal Maliga, Ph.D.
Waksman InstituteRutgers University190 Frelinghuysen RoadPiscataway, NJ 08854-8020United States
Pal Maliga received his Ph.D. degree in 1972 from theUniversity of Szeged, Hungary. His early research at theBiological Research Center in Szeged focused on cell biologyapproaches to manipulate the plastid and mitochondrialgenomes of higher plants.
In 1988, he was appointed Professor of Genetics atRutgers, The State University of New Jersey. Since then hisresearch group at the Waksman Institute has developed thetechnology for plastid transformation in higher plants usingtobacco as a model system. They currently use plastid transfor-mation to characterize the plastid transcription machinery andto understand the rules of mRNA translation, RNA editing andproduction of recombinant proteins in plastids. In addition, theyare directing their efforts to extend the technology of plastidtransformation to Arabidopsis and rice.
Transplastomic Technology for Safer and Better Transgenic Crops
Plastids are plant cellular organelles with their own genome~150 kb in size, and have a prokaryotic-type transcription andtranslation machinery. The plastid type that differentiates forphotosynthesis, the chloroplast, is the best characterized. A leafcell typically carries about 100 chloroplasts, each having about100 identical genome copies. It is a challenge to obtain geneti-cally stable transplastomic lines by gradually changing all thegenome copies.
A unique genetic feature of plastids in most crops is thatplastids are transmitted to the seed progeny by the maternal par-ent only. As a consequence, plastid transgenes, including genesencoding herbicide resistance, are not transmitted by pollen. Incontrast, the spread of herbicide resistance genes via pollen is aconcern in crops with weedy relatives such as oilseed rape andrice where the genes are incorporated in the nucleus. Better con-trol of transgene flow by incorporating herbicide resistance inthe plastid genome will extend the life of the environment-friendly herbicides. Resistance to glyphosate and Basta areexamples of successfully providing protection against commer-cial herbicides by plastid transgenes.
Production of recombinant proteins in chloroplasts is apromising and an unexplored area. Recombinant proteins fromplastid transgenes may accumulate in leaves up to a level of20% to 25% of total soluble cellular protein. The high levelaccumulation of recombinant protein is due to a high transgenecopy number and in addition, the availability of expression sig-nals that will predictably yield proteins in the range of 0.1% to>20%. The expression of recombinant proteins in chloroplastsholds a great potential for low-cost production of vaccines, anti-bodies, and industrial enzymes. The recombinant protein levelsare sufficiently high so as to allow the direct use of leaf proteinextracts in some applications without further purification.Engineering of agricultural input traits such as insecticidal prop-erties can also benefit from high expression levels, exemplifiedby production of the insecticidal Bacillus thuringiensisendo-toxin in chloroplasts.
Plastid transformation is a difficult technology. Currently,it is routinely done only in tobacco. The present challenge is toextend the technology to all major crops. Plastid transformationhas been reduced to practice in potato, and it appears that riceand relatives of oilseed rape also yield to persistent efforts.
© 1999 Nature America Inc. • http://biotech.nature.com©
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