ultrafast real-time digital autoradiography solutions€¦ · courtesy of dr. alain schweitzer,...
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Acquire highly detailed images in a few hours
See the first results in minutes
Follow acquisition in Real-Time
ultrafast real-time
digital autoradiography solutions
Keep your research moving
- All commonly used beta emitting radioisotopes :3H, 14C, 32P, 35S, 125I
- Positron emitting (PET) isotopes
- Secondary beta emissions from several gamma emitting radioisotopes: 123I, 131I, 99mTc, 201Tl, 111In.
Courtesy of Janssen Research Foundation
Courtesy of Dr. Alain Schweitzer, Novartis
Linear dynamic range of the BetaIMAGER
Key Benefit:
Detect and quantify both high and low
sample concentrations
in a single data acquisition
Real-time autoradiography: distribution of D1 receptors
in rat brain striatum following tritiated ligand bindingKey Benefits:
See the irst results in minutes
See data accumulating in real-time
Avoid under/over exposure
1min 5min 15min 60min
High sensitivity & rapid image acquisition
accurate quantification
real-time digital autoradiography
Langlois and al,, 2001
200 x 250 mm maximum ield of view
Image multiple tissue sections
- up to 15 microscopy slides
- or a single larger sample (e.g.:TLC plate)
Optical zoom mode:
50 micron spatial resolution for smaller sample area Courtesy of A. Molatt and P. Mitchell, Pizer
FOV: 200 x 250 mm
FOV: 75 x 100 mm
QWBA rat section labeled with tritium
BetaIMAGERtm tRacer
Key Benefit:
Unmatched sensitivity cuts
your data acquisition time
from weeks to hours
Rat Brain sections labeled with a tritiated probe
12 hour data acquisition 12 week exposure
Key Benefit:
Detect ultra-low levels of bound
radiolabeled ligand
Use minimal amounts of radiolabeled
compound
Reduce radioactive waste
Minimize experimental costs
A study of NK3 receptor occupancy
The sensitivity of the BetaIMAGER TRACER system allows visuali-
zation and quantitative analysis of tritiated senktide binding to
NK3 receptors in a matter of hours when other autoradiography
methods would have taken months. Langlois and al., 2001
in situ hybridization
high throughput screening electrophoresis gels & blots
receptor binding studies
wide range of applications
turns autoradiography into a fast and easy routine
Large sample size & high resolution in one system
whole body imaging TLC plates
BetaIMAGER TRACERFilm Autoradiogram
excellent multi-label imaging capability
Multiplex various ligands and/or tracers in a single experiment
Separate and accurately discriminate between radioisotopes based on either differences
in energy (2 radioisotopes), or decay rates (up to 3 radioisotopes)
A fast and sensitive method for simultaneously determining the distribution of several
molecules in a single tissue section
Single
acquisition
Isotopic
separation Fusion
Establishing the histopathology of
myocardial infarct in a rat based model
111In-DTPA (Blue): Detects increased extracellular luid within ibrotic areas.
99mTc-Sestamibi (Green): Detects both the ischemic
and viable tissue surrounding the necrosis based on
levels of tissue perfusion.
18FDG (Red): Detects all tissue with an active glycolitic
metabolism
A S. Poussier and al, 2005
Ex vivo imaging of [11C]5 retention in 22 month old female tg/tg APP/ PS1 animals
following bolus injection of a [11C]5/[3H]1a cocktail. Yellow scale bars =100 μm.Yousei et al., 2010
For sections mounted on standard glass microscope slides
(imaging area is 24 x 32 mm)
Four position sample stage
Automated sequential data acquisition for all four samples
Spatial resolution of 10 μm can be achieved with 3H labelling
Possible combinations of energy based dual-detection radioisotope pairs include 3H/14C,
3H/35S, 3H/32P or 33P and for decay rate based multi-label combinations ,99mTc/ 111In/18F.
the high resolution digital autoradiography Solution
BetaIMAGERtm dFine
Co-localization of various receptors, ligands
Ex vivo imaging before/after in vivo μPET/μSPECT
microarraysreceptor binding assays
wide range of applications
BetaIMAGERtm
tRacer
BetaIMAGERtm
dFine
Technology
Detection Gaseous avalanche chamber Scintillating foil
Camera Intensiied CCD Camera Intensiied CCD Camera
Performance
Pixel Resolution 5µm 1µm
Spatial ResolutionFrom 50µm to 400µm depending on isotope,
zoom and sample
10µm to 25µm depending on
isotope
Field of View (FOV)
15 microscope slides: 200mm x 250 mm
6 microscope slides: 120mm x 160 mm
2 microscope slides: 75mm x 100 mm
Half microscope slide: 25 mm x 33 mm
24 mm x 32 mm
Linearity Linear response over a dynamic range of 104 Linear response over a dynamic range of 104
Multilabelling Capability
Energy Separation2 isotopes chosen from 3 different families of beta emitters : low energy (3H); medium energy
(14C, 35S, 33P, 99mTc,…) and high energy (32P, 18F, 11C,…)
Radioactive Decay
Separation
Up to 3 Beta emitting isotopes (including PET) with suficiently different radioactive decay rates can be imaged simultaneously and then separated
Cross talk (typical) 5 % for a 3H/14C separation 1 % for a 3H/14C separation
Sample Holder
Standard
Tritiated membranes
Non Tritiated membranes (14C, 35S, 33P,…)
15 microscope slides
4 position Automated microscope slide
changer
On request
Multi-isotopes sample holder
1 slide (127 x 178 mm) special sample holder
4 slides (120 x 170 mm) special sample holder
9 slides (51 x 76 mm) special sample holder
12 slides (38 x 76 mm) special sample holder
Softwares
Image Acquisition Beta-T Acquisition Software Beta-D Acquisition Software
Image Analysis M3Vision Analysis Software M3Vision Analysis Software
Data FormatsBitmap, JPEG, illustration & quantitative TIFFs,
ASCII
Bitmap, JPEG, illustration & quantitative TIFFs,
ASCII
System Requirements
Operating System Windows XP/7 Windows XP/7
Power Consumption Maximum: 250 W @ 230 V or 115 V Maximum: 50 W @ 230 V or 115 V
Dimensions 75x70x160 cm (WxDxH) 40x45x75 cm (WxDxH)
Weight 145 kg 35 kg
Technical Specifications
BIOSPACE LAB SA, France
11 Rue de Chenival, 95690 Nesles-la-Vallée, Francet +33 (0) 1 30 34 75 16f +33 (0) 1 30 34 72 21 [email protected]
BIOSPACE LAB Inc., USA
185 Alewife Parkway #410Cambridge, MA02138, USA t + 1 201 785 [email protected]
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