unit 1: the mystery #2 100 200 300 400 500 100 200 300 400 500 100 200 300 400 500 100 200 300 400...

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Unit 1: The Mystery #2 100 200 300 400 500 100 200 300 400 500 100 200 300 400 500 100 200 300 400 500 100 200 300 400 500 Careers Grab Bag Restriction Enzymes Gel Electrophor esis Confidentia lity

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Page 1: Unit 1: The Mystery #2 100 200 300 400 500 100 200 300 400 500 100 200 300 400 500 100 200 300 400 500 100 200 300 400 500 Careers Grab BagRestriction

Unit 1: The Mystery #2

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Careers Grab Bag Restriction Enzymes

Gel Electrophoresis

Confidentiality

Page 2: Unit 1: The Mystery #2 100 200 300 400 500 100 200 300 400 500 100 200 300 400 500 100 200 300 400 500 100 200 300 400 500 Careers Grab BagRestriction

QuestionAnswer

Careers-100

• ANSWER: The individual performs DNA profiling.

• QUESTION: Who is a DNA analyst?

Page 3: Unit 1: The Mystery #2 100 200 300 400 500 100 200 300 400 500 100 200 300 400 500 100 200 300 400 500 100 200 300 400 500 Careers Grab BagRestriction

QuestionAnswer

Careers-200

• ANSWER: A physician who performs an autopsy when death may be accidental or violent.

• QUESTION: Who is a medical examiner?

Page 4: Unit 1: The Mystery #2 100 200 300 400 500 100 200 300 400 500 100 200 300 400 500 100 200 300 400 500 100 200 300 400 500 Careers Grab BagRestriction

QuestionAnswer

Careers-300

• ANSWER: This individual performs scientific tests on bodily fluids and tissue samples to identify any drugs or chemicals present in the body.

• QUESTION: Who is a toxicologist?

Page 5: Unit 1: The Mystery #2 100 200 300 400 500 100 200 300 400 500 100 200 300 400 500 100 200 300 400 500 100 200 300 400 500 Careers Grab BagRestriction

QuestionAnswer

Careers-400

• ANSWER: This individual provides medical treatment at the scene of a crime or on the way to a hospital.

• QUESTION: Who is an emergency medical technician (EMT)?

Page 6: Unit 1: The Mystery #2 100 200 300 400 500 100 200 300 400 500 100 200 300 400 500 100 200 300 400 500 100 200 300 400 500 Careers Grab BagRestriction

QuestionAnswer

Careers-500

• ANSWER: This individual pronounces death and signs the death certificate.

• QUESTION: Who is a coroner?

Page 7: Unit 1: The Mystery #2 100 200 300 400 500 100 200 300 400 500 100 200 300 400 500 100 200 300 400 500 100 200 300 400 500 Careers Grab BagRestriction

QuestionAnswer

• ANSWER: This person is responsible for notifying the appropriate responders.

• QUESTION: What is a 911 operator?

Grab Bag-100

Page 8: Unit 1: The Mystery #2 100 200 300 400 500 100 200 300 400 500 100 200 300 400 500 100 200 300 400 500 100 200 300 400 500 Careers Grab BagRestriction

QuestionAnswer

Grab Bag-200

• ANSWER: Demonstrating reliability, give credit to the author, as a source for additional information.

• QUESTION: What are reasons to document (cite) your sources?

Page 9: Unit 1: The Mystery #2 100 200 300 400 500 100 200 300 400 500 100 200 300 400 500 100 200 300 400 500 100 200 300 400 500 Careers Grab BagRestriction

QuestionAnswer

Grab Bag-300

• ANSWER: Larger pieces of DNA will travel _____________ than small ones in a gel.

• QUESTION: What is slower?

Page 10: Unit 1: The Mystery #2 100 200 300 400 500 100 200 300 400 500 100 200 300 400 500 100 200 300 400 500 100 200 300 400 500 Careers Grab BagRestriction

QuestionAnswer

Grab Bag-400

• ANSWER: To help determine sizes of unknown DNA fragments.

• QUESTION: Why do scientists load DNA of known sizes into the agarose gel?

Page 11: Unit 1: The Mystery #2 100 200 300 400 500 100 200 300 400 500 100 200 300 400 500 100 200 300 400 500 100 200 300 400 500 Careers Grab BagRestriction

QuestionAnswer

Grab Bag-500

• ANSWER: This is the name given to the morgue assistant that helps prep the body for autopsy.

• QUESTION: What is the diener?

Page 12: Unit 1: The Mystery #2 100 200 300 400 500 100 200 300 400 500 100 200 300 400 500 100 200 300 400 500 100 200 300 400 500 Careers Grab BagRestriction

QuestionAnswer

Restriction Enzymes-100

• ANSWER: A degradative enzyme that recognizes specific nucleotide sequences and cuts up DNA.

• QUESTION: What is a restriction enzyme?

Page 13: Unit 1: The Mystery #2 100 200 300 400 500 100 200 300 400 500 100 200 300 400 500 100 200 300 400 500 100 200 300 400 500 Careers Grab BagRestriction

QuestionAnswer

Restriction Enzymes-200• ANSWER: Differences in DNA sequence

on homologous chromosomes that can result in different patterns of restriction fragment lengths (DNA segments resulting from treatment with restriction enzymes).

• QUESTION: What are Restriction Fragment Length Polymorphisms (RFLPs)?

Page 14: Unit 1: The Mystery #2 100 200 300 400 500 100 200 300 400 500 100 200 300 400 500 100 200 300 400 500 100 200 300 400 500 Careers Grab BagRestriction

QuestionAnswer

Restriction Enzymes-300

• ANSWER: Segments of DNA cuts with restriction enzymes are measured in number of this.

• QUESTION: What is number of base pairs?

Page 15: Unit 1: The Mystery #2 100 200 300 400 500 100 200 300 400 500 100 200 300 400 500 100 200 300 400 500 100 200 300 400 500 Careers Grab BagRestriction

QuestionAnswer

Restriction Enzymes-400

• ANSWER: Where restriction enzymes are found.

• QUESTION: What is bacteria?

Page 16: Unit 1: The Mystery #2 100 200 300 400 500 100 200 300 400 500 100 200 300 400 500 100 200 300 400 500 100 200 300 400 500 Careers Grab BagRestriction

QuestionAnswer

Restriction Enzymes-500

• ANSWER: The restriction enzyme EcoR1 cuts a strand of DNA at four restriction sites resulting in this many RFLPs.

• QUESTION: What are five?

Page 17: Unit 1: The Mystery #2 100 200 300 400 500 100 200 300 400 500 100 200 300 400 500 100 200 300 400 500 100 200 300 400 500 Careers Grab BagRestriction

QuestionAnswer

Gel Electrophoresis-100

• ANSWER: In gel electrophoresis, DNA shows up as these on the gel.

• QUESTION: What are bands?

Page 18: Unit 1: The Mystery #2 100 200 300 400 500 100 200 300 400 500 100 200 300 400 500 100 200 300 400 500 100 200 300 400 500 Careers Grab BagRestriction

QuestionAnswer

Gel Electrophoresis-200

• ANSWER: In gel electrophoresis, DNA is fragments are separated based on this.

• QUESTION: What is number of base pairs?

Page 19: Unit 1: The Mystery #2 100 200 300 400 500 100 200 300 400 500 100 200 300 400 500 100 200 300 400 500 100 200 300 400 500 Careers Grab BagRestriction

QuestionAnswer

Gel Electrophoresis-300

• ANSWER: During gel electrophoresis, this causes DNA to move out of the wells and through the gel.

• QUESTION: What is the attraction of the negative DNA, from the phosphate groups, to the positive electrode?

Page 20: Unit 1: The Mystery #2 100 200 300 400 500 100 200 300 400 500 100 200 300 400 500 100 200 300 400 500 100 200 300 400 500 Careers Grab BagRestriction

QuestionAnswer

Gel Electrophoresis-400

• ANSWER: During gel electrophoresis, this size fragments migrate further in the gel due to less friction with the agarose.

• QUESTION: What are smaller fragments?

Page 21: Unit 1: The Mystery #2 100 200 300 400 500 100 200 300 400 500 100 200 300 400 500 100 200 300 400 500 100 200 300 400 500 Careers Grab BagRestriction

QuestionAnswer

Gel Electrophoresis-500

• ANSWER: This is added to a gel to separate different size fragments on DNA.

• QUESTION: What is an electric current?

Page 22: Unit 1: The Mystery #2 100 200 300 400 500 100 200 300 400 500 100 200 300 400 500 100 200 300 400 500 100 200 300 400 500 Careers Grab BagRestriction

QuestionAnswer

Confidentiality-100

• ANSWER: A comprehensive set of standards and practices designed to give patients specific rights regarding their personal health information.

• QUESTION: What is the Health Insurance Portability and Accountability Act (HIPAA)?

Page 23: Unit 1: The Mystery #2 100 200 300 400 500 100 200 300 400 500 100 200 300 400 500 100 200 300 400 500 100 200 300 400 500 Careers Grab BagRestriction

QuestionAnswer

Confidentiality-200

• ANSWER: Individually identifiable health information such as demographic data, mental/health condition, and payment information is considered this.

• QUESTION: What is protected health information (PHI)?

Page 24: Unit 1: The Mystery #2 100 200 300 400 500 100 200 300 400 500 100 200 300 400 500 100 200 300 400 500 100 200 300 400 500 Careers Grab BagRestriction

QuestionAnswer

Confidentiality-300

• ANSWER: It is ok to do this when trying to prevent child abuse, domestic violence, or the outbreak of a disease.

• QUESTION: What is break confidentiality?

Page 25: Unit 1: The Mystery #2 100 200 300 400 500 100 200 300 400 500 100 200 300 400 500 100 200 300 400 500 100 200 300 400 500 Careers Grab BagRestriction

QuestionAnswer

Confidentiality-400

• ANSWER: These individuals should keep patient information private.

• QUESTION: Who is any medical personnel and those who have access to medical records?

Page 26: Unit 1: The Mystery #2 100 200 300 400 500 100 200 300 400 500 100 200 300 400 500 100 200 300 400 500 100 200 300 400 500 Careers Grab BagRestriction

QuestionAnswer

Confidentiality-500

• ANSWER: Violating patient confidentiality can possibly result in serious fines, criminal prosecution, and this.

• QUESTION: What is loss of employment?