universidad nacional autónoma de méxico...box of pat5 by anti-ptstbp1-c antibody. analysis of core...
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Analysis of TATA binding Protein 1 (TBP1) of Taenia solium and core promoter elements In taeniidae
family genes.
Oscar Rodríguez Lima
Universidad Nacional Autónoma de México
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Taenia solium Human
(Definitive Host)
Infected swine
meat consumption
Taeniosis
Swine
(Intermediate host)
T. solium eggs
comsumption
Swine cysticercosis
T. solium eggs
compsumption
Human cysticercosis
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Neurocysticercosis
• Its a health problem in most of developing
countries (Fleury A. et al., 2010).
• Its the most important parasitic disease of the
central nervous system, and the first cause of
late epilepsy (Esquivel-Velázquez M. et al., 2011).
• In Mexico is still prevalent, represents the
2.5% of the total patients of National Institute of
Neurology and Neurosurgery (Fleury A. et al., 2010)
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Transcription by RNA Pol II
• The protein coding-genes are transcribed by RNA Pol II.
• RNA Pol II required the formation of pre-Initiation Complex in the core promoter.
Butler J. E. F. & Kadonaga J. T., 2002
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TATA Inr
-30
+1
+20
ATG TGAATTC TATATTTG Ts2-CysPrx
Inr
+1
+23
ATG TGCTCGT TsCu,Zn-SOD
TATA Inr
-30
+1
+44
ATG GCAACTT TATATAAC Actin (pAT5)
Campos A.et al, 1990.
Vaca-Paniagua F. et al, 2009.
Parra-Unda R. et al, 2011.
Transcription in Taenia genus. • Litle its know about transcription in this genus.
• Only a few core promoter sequences have been published.
DPE
+28
GGCTGT
+33
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TATA-box Binding Protein (TBP)
NH2-terminal domain
COOH-ter domain DNA binding site
Davidson I., 2003. Juven-Gershon T. & Kadonaga J. T., 2010
TATAWAAR
W = A or T R = A or G
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Multiple
alignment of
diferent
TBP1s
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TsTBP1 homology model.
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Identification
of TsTBP1 by
western blot
and confocal
microscopy.
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TsTBP1 interaction with two putative TATA-box
pAT5 Ts2-CysPrx
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Probes used in EMSA
pAT5 TATA-box 5’ – CCCAAATCTTATATAAACCGTGGGT – 3’
3’ – GGGTTTAGAATATATTTGGCACCCA – 5’
Ts2-CysPRX TATA-box 5’ – GCGCTTCGCTATATTTGGCGGTAAG – 3’
3’ – CGCGAAGCGATATAAACCGCCATTC – 5’
Consenso TATA-box 5’ – AAGGGGGGCTATAAAAGGGGGTGGG – 3’
3’ – TTCCCCCCGATATTTTCCCCCACCC – 5’
Consenso TATA-box
mutada
5’ – AAGGGGGGCTGTGAAAGGGGGTGGG – 3’
3’ – TTCCCCCCGATATTTTCCCCCACCC – 5’
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TsTBP1 binding inhibition to teh TATA-box of pAT5 by anti-pTsTBP1-C antibody.
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Analysis of core promoter of TsTBP1 and pAT5 coding genes
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Identification of TsTAF6 and homology model.
Antonospora
locustae
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Core promoter analysis in taeniidae family genes.
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Thank you! Oscar Rodríguez-Lima Molecular Biology of Parasites Lab Universidad Nacional Autónoma de México