university of pune [4363]-274 t. e. (biotechnology...

UNIVERSITY OF PUNE[4363]-274

T. E. ( Biotechnology) Examination- May 2013COMPUTATIONAL TECHNIQUES AND BIOSTATISTICS

(2008 Course)Total No. of Questions : 12 [Total No. of Printed Pages :3]

[Time : 3 Hours] [Max. Marks : 100]

(1) Answer any three questions from Section I and three questions from Section II

(2) Figures to the right indicate full marks.(3) Use of Programmable calculator is not allowed.(6) Assume suitable data, if necessary.

SECTION-IQ1.

a) Describe the working procedure for fitting a straight line y=a+bx.

following terms. [8]

b) Find the values of A and Y which best fit the equation A=mY +x by using

the following data. [8]A 12 15 21 25Y 50 70 100 120

ORQ2.

a) Velocity and time are related by the equation of the form v=a t b . find “a”

and “b” by using the following data. [8]V 350 400 500 600

t 61 26 7 26

b) Describe the working procedure for fitting the curve of the form [8]

Q3.

a) Evaluate ∆10[(1−ax)(1−b x2

)(1−c x3)(1−d x4

)] [6]

b) Assuming that the following values of y belong to a polynomial of degree 4, complete the next three values. [10]

x 0 1 2 3 4 5 6 7

y 1 -1 1 -1 1 - - -

ORQ4.

a) Following data gives the values of x and y . Find f (12) using

Lagrange’s formula. [8]x 1.2 2.1 2.8 4.1 4.9 6.2

y 4.2 6.8 9.8 13.4 15.5 19.6

b) What is Interpolation? What are the drawbacks of Lagrange’s formula? How can it be used corrected? [8]

Q5.a) The velocity changes with time of a car as per the given data. [9]

t 2 4 6 8 10 12 14 16 18 20

v 10 18 25 29 32 20 11 5 2 0

Estimate the distance travelled by the car in 20 min.

b) By using Simpson’s 1/3rd rule find the value of the function e− x2

dx by

taking seven coordinates. [9]OR

Q6. What is Numerical Integration? What do you mean by Quadrature? Deduct Weddle’s rule by using Newton Cote’s Quadrature formula? [18]

SECTION-IIQ7.

a) Find the real root of y=3x−cosx−1 by Newton’s method correct too four

decimal places. [8]b) Use the method of False position to find the fourth root of 32 correct to three

decimal places. [8]OR

Q8.a) Define Quasi random Sampling method? How can it be formed? When is it

used? What is the working procedure adopted for sampling under this method? [10]

b) Define the following terms. [6]1) Representative Sample 2) Bias3) Statistics

Q9. Explain the factors considered while choosing a suitable diagram for the representation of any data. [16]

ORQ10. Write detailed notes on Ogives. Why is it so called? Describe the methods of constructive Ogive? What are its uses? [16]

Q11.a) A Personal Manager is interested in trying to determine whether absenteeism

is greater on the day of the week than on another. His records for the past year show this sample distribution. [10]Day of week mon tue wed thu friNo of Absentees 66 51 54 48 75Test whether the absence is uniformly distributed over the week.

b) The mean produce of wheat of a sample of 100 fields is 200lb/acre with a standard deviation of 10lb. Another sample of 150 fields gives the mean at 220 with a standard deviation of 12lb. Assuming the standard deviation of the mean field at 1lb of the universe, find 1% level if the two results are consistent. [8]

ORQ12.

a) Prices of shares of a company on different days in a month were found to be 66,65,69,70,69,71,70,63,64,68. Discuss whether the prices of the shares be 65. Table value of t=2.262. [9]

b) Find the average rate of increase in population which in the first decade has increased by 20%, in second decade by 30% and in the third decade by 40%.

[9]

1

PUNE UNIVERSITY

[4363]-277

T. E. Biotechnology, Examination - 2013

Fermentation Technology - II

(2008 Course)

[Total No. of Questions: 12] [Total No. of Printed Pages: 2]

[Time: 3 Hours] [Max. Marks: 100]

Instructions:

(1) Answer any three questions from each section-I and any

three from section-II.

(2) Figures to the right indicate full marks.

(3) Use of programmable calculator is allowed.

(4) Assume suitable data, if necessary.

SECTION-I

Q. 1. Explain the factors effecting diffusion in Bio processing. (16)

OR

Q. 2. Write short notes on the following. (16)

i. Impeller Reynolds number

ii. Power number

Q. 3. Write short notes on Bubble driven Bioreactors. (16)

OR

Q. 4. Briefly explain the Construction and working of Continuous (16)

Countercurrent Decanter.

Q. 5. Define Adsorption. What is its application in Biotech Industries? (18)

Explain how the nature of adsorbents affects the efficiency of a process?

OR

Q. 6. Prove that two stage crosscurrent treatment of liquid solution by (18)

Contact filtration when the adsorption isotherm is linear, the least

Total adsorbent results if the amounts used in each stage are equal.

2

SECTION-II

Q. 7. List out the equipments used for leaching of fine solids. Explain the (16)

Constructions and working of Ball man Extractor.

OR

Q. 8. Soybean oil is to be extracted from granulated soybean seeds in a (16)

Countercurrent extraction unit using ethyl ether as a solvent. The

Mass flow rate of halibut seeds to the unit is 350kg/hr. The fresh seeds

contain 20% oil and are to be extracted to a composition of 1% of oil. The

Solvent containing 2% oil to be used as used as a solvent is fed to the unit at

The rate 250kg/hr. Determine the number of theoretical stages required.

And % recovery of oil.

Concentration 0 0.1 0.2 0.3 0.4 0.5 0.6

Solution retained 0.28 0.34 0.4 0.47 0.55 0.66 0.8

Q. 9. What is membrane fouling? Explain the factors which decrease the (16)

Flux through a membrane. How can it be prevented?

OR

Q. 10. Explain the parameters which are used to assess Pervaporation (16)

Process?

Q. 11. Define liquid Extraction. What are the fields of its usefulness? (18)

OR

Q. 12. What are the factors affecting the Choice of a solvent in any extraction (18)

Operation? Explain how each factor actually affects the efficiency of

the operation?

UNIVERSITY OF PUNE

[4363-271]

T.E.(Biotechnology) Examination,2013

GENETIC ENGINEERING

(2008 pattern)

Time-Three hours Maximum Marks-100

[Total No. of Question=12] [Total no. of printed pages= 3]

Instructions:

(i)Answer Q. No. 1 or Q. No. 2, Answer Q. No. 3 or Q. No. 4, Answer Q. No.

5or Q. No. 6, Answer Q. No. 7 or Q. No. 8, Answer Q. No. 9 or Q. No. 10,

Answer Q. No. 11 or Q. No. 12.

(ii)Answers to the two sections should be written in separate answer books.

(iii)Draw neat diagrams wherever necessary.

(iv)Figures to the right indicate full marks.

SECTION-I

Q.1. Describe and discuss in detail the procedure for Southern Blotting with a neat

labelled diagram also give its application. (18)

OR

Q.2 Describe RTPCR and give its application. Enlist and describe the usage of the

various enzymes involved in RTPCR and PCR. (18)

Q.3 What are Cloning vectors, explain their significance in genetic engineering?

Describe a model plasmid cloning vector? (16)

OR

Q.4 What are expression vectors? Give their significance , and describe a typical

expression vector. (16)

Q.5 Explain the concept of genomic libraries, how are they constructed? What are

cDNA libraries? (16)

OR

Q.6 Write in detail the steps involved in cloning a protein product till mass production.

(16)

SECTION-II

Q.7 Answer the following (9 marks each) (18)

(i)Explain in detail screening of recombinants using the blue white selection

method.

(ii)Describe PCR cloning.

OR

Q.8 Explain the dideoxy sequencing method in detail, with a neat diagram. (18)

Q.9 What are the different ways by which genomic material is transferred in bacteria?

Explain with respect to Conjugation and transformation. (16)

OR

Q.10 How are transgenics organisms constructed? What are their significance and

applications in biotechnology? (16)

Q.11 Answer the following (8 marks Each) (16)

(i)Explain the application of recombinant DNA Technology, in agriculture

with a detailed analysis of golden rice.

(ii)What is Human Genome Project?How was it impacted biology and

biotechnology?

OR

Q.12 Write a short note on (4 marks each) (16)

(i) RFLP

(ii) AFLP

(iii) DNA diagnostics

(iv) Hep B

1

UNIVERSITY OF PUNE

[4363]-272

T. E. (Biotechnology] - Examination - 2013

FERMENTATION TECHNOLOGY-I

( 2008 Course)

[Total No. of Questions:] [Total No. of Printed pages :2]


Instructions :

(1) Answer any three questions from each section.

(2) Answers to the two sections should be written in

separate answer-books.

(3) Black figures to the right indicate full marks.

(4) Neat diagrams must be drawn wherever necessary.

(5) Use of logarithmic tables, slide rule, Mollier charts,

electronic pocket calculator and steam tables is allowed.


SECTION I

Q.1] What is Fermentation Technology? Explain application of [16]

fermentation technology in detail.

OR

Q.2] Give example of classical fermentation systems with example and [16]

explain the inoculum preparation methods in detail.

Q.3]What is medium optimization? Explain in detail any one method [16]

of medium optimization.

OR

Q.4] Explain different types of media. Give importance of different [16]

media components.

Q.5] Describe in detail activities of acetic acid bacteria and explain [18]

production of vinegar

OR

2

Q.6] Explain following alcoholic beverages production process: [18]

a] Wine

b] Beer

SECTION II

Q.7] Mention the list of antibiotics produced by application of [16]

fermentation technology. Explain production process of one of the antibiotic

from your list.

OR

Q.8] What do you mean by immobilization? What is the application [16]

of immobilization in fermentation technology? Explain one application of

enzyme immobilization in detail.

Q.9a] What is mean by solid state fermentation [SSF]? Explain [8]

application of SSF in detail.

Q.9b] Enlist the type of fermenters getting utilized in submerged [8]

fermentation process. Explain one of them in detail.

OR

Q.10] Explain following modes of operation in stirred tank reactors: [16]

a] Discontinuous batch operations

b] Continuous operation

c] Semi-continuous operations

d] Periodic fed-batch operations

Q.11] What is Scale Up? Explain the concept with suitable case study. [18]

OR

Q.12] Write short note on: [18]

a] GMP

b] Downstream Processing

c] Fermentation efficiency

1

UNIVERSITY OF PUNE

[4363]-273

B. E. ( Biotechnology) Examination - 2013

HEAT TRANSFER (315403)

(2008 Pattern)


[Total No. of Questions:12] [Total No. of Printed Pages :3]

Instructions :

(1) Answers three questions from Sections I and three questions from

Section II.

(2) Answers to the two sections should be written in separate

answer-books.

(3) Black figures to the right indicate full marks.

(4) Neat diagrams must be drawn wherever necessary.

(5) Use of scientific calculator is allowed.


SECTION-I

Q1 a) State and explain Buckingham pi-theorem. [6]

b) What are the different modes of heat transfer? Explain with suitable [10]

example.

OR

Q2 a) What is the significance of dimensional analysis? Explain its role in [6]

data reduction.

b) For convection heat transfer for flow of fluid through a tube, derive the [10]

following equation using Buckingham pi-theorem.

Nu=c (Re)m (Pr)

n

Where c, m and n are constants.

Q3 a) State and explain Fourier’s law of conduction. [4]

b) What are fins? Define fin effectiveness and explain different types of fins [10]

(at least 3) with neat sketches.

c) Explain the concept of steady state and unsteady state heat transfer. [4]

OR

2

Q4 a) Find the heat flow rate per unit length through a thick pipe of outer [4]

diameter 4 cm and inner diameter 2 cm. (Data: Thermal conductivity of

pipe=0.58 W/m-K; Temperature of inner wall of the pipe=70°C and

Temperature of outer wall of the pipe=100°C).

b) Derive the equation to find maximum temperature distribution in an [10]

infinitely long solid cylinder of radius R in which heat is being generated at a

uniform rate of ‘q’ units per unit volume. The heat transfer coefficient at the

surface is ‘h’ and the ambient temperature is ‘𝑇𝑓’.

c) What is thermal diffusivity? Explain its significance. [4]

Q5 a) Distinguish between natural and forced convection with suitable [4]

example.

b) What are types of boiling? Explain the pool boiling curve. [8]

c) Define condensation and types of condensation. [4]

OR

Q6 a) A steel pipe 25 mm ID and 33 mm OD and insulated with rock wool [10]

carries steam at 178°C. If the surrounding air temperature is 21°C, calculate the

rate of heat loss from one meter length of pipe. The thickness of insulation is 38 mm.

Thermal conductivity of steel and rock wool are 10.74 and 0.0418 cal/sec-m2 °C

respectively. The inside and outside heat transfer coefficients are 1356.17 and 2.7133

cal/sec m2 °C respectively. Contact resistance between the pipe and insulation may be

neglected.

b) Explain the concept of thermal boundary layer. [4]

c) Explain the significance of Prandtl number. [2]

SECTION-II

Q7 a) Explain any one case of radiation between two surfaces and derive the [8]

necessary equation for the same

b) State and explain i) Stefan Boltzman law ii) Planck’s law iii) Wien’s law [8]

iv)Kirchoff’s law

OR

Q8 a) Define i) emissivity ii) gray body iii) opaque body iv) black body [4]

b) What is transmissivity, absorptivity and reflectivity? Based on these explain [8]

the specular and diffuse reflection.

c) Explain the concept of shape factor. [4]

3

Q9 a) Explain with neat sketch working of direct and indirect contact heat [6]

exchanger.

b) Derive the equation for log mean temperature difference for parallel flow [10]

heat exchanger.

OR

Q10 a) Draw the sketch of 1-2 shell and tube heat exchanger and explain its [6]

working.

b) What are the factors that cause fouling? Explain the effect of fouling on heat [4]

transfer.

c) Water enters a two-fluid heat exchanger at 55°C and leaves at 85°C. Hot [6]

gases enter at 305°C and leaves at 160°C. If the total heat transfer area is

500 𝑚 2and the overall heat transfer coefficient is 600 kcal/hr , 𝑚2°C, determine

the total heat transferred per hour for i) parallel flow and ii) counter flow of the

two fluids.

Q11 a) Define the performance measures for evaporators and explain how [4]

they are interrelated.

b) Explain different methods of feeding in multiple effect evaporators. [10]

c) Explain the working principle of circulation evaporators with neat sketch. [4]

OR

Q12 a) What are the resistances to heat transfer in evaporators? Explain with [6]

neat sketch.

b) What is boiling point elevation? State the Duhring’s rule. [4]

c) An evaporator is to be fed with 5000kg/h of solution containing 10% solute [8]

by weight. The feed at 40°C is to be concentrated to a solution containing 40% by

weight of the solute under an absolute pressure of 1.03 kg/𝑐𝑚2. Steam is available

at an absolute pressure of 3 atmosphere (saturation temperature of 134°C). The

overall heat transfer coefficient is 1500 kcal/hr𝑚2°C. Calculate i) heat transfer area

that should be provided ii) the steam requirement.

Temperature, °C Enthalpy, kcal/kg

Vapor Liquid

40 613.5 40.5

100 639.2 100.0

134 651.4 134.4

of 3

[Total No. of Questions:12] [Total No. of Printed Pages: 3]

UNIVERSITY OF PUNE

[4364]-641

T. E. (Biotechnology)(Semester-I) Examination - 2013

(Mass Transfer)(315462)( 2008 Course)


Instructions:

1 Answer any three questions from each section.

2 Assume suitable data, if necessary.

3 Figures to the right indicate full marks.

4 Use of programmable calculator is not allowed.

SECTION -I

Q.1 Derive the equations for the following with proper

nomenclature and assumptions

i. Steady State molecular diffusion in liquids at

rest.

ii. Steady State equimolal.

16

OR

Q.2 Discuss in detail Theories of Mass Transfer. 16

Q. 3 A Write short notes on Distillation Column Internals. 8

B 100 moles of Benzene and Toluene containing 50

mole% Benzene in the residue is 33% by mole.

Calculate the total moles of the mixture distilled? Take

relative volatility value as 2.4

8

OR

Q. 4 A Define Fenske’s Equation. What are its applications? 8

B Data of mole fraction of carbon disulphide in the liquid

and in vapour evolved from the mixture during

distillation of carbon disulphide carbon tetrachloride

mixture as follows.

x 0 0.2 0.4 0.6 0.8 1

y 0 0.445 0.65 0.795 0.91 1

Determine graphically number of theoretical plates

required.

8

of 3

Q. 5 A Derive the operating line equation for Equilibrium

distillation and deduce the operating lines for various

ranges of feed values.

9

B Write short notes on the following with suitable

examples.

i. Minimum Boiling Azeotrope

ii. Total Condenser

9

OR

Q. 6 What is Reflux ratio? How does it affect the purity of

the product? Write short notes on Minimum and Total

Reflux ratio. Which one works best in order to achieve a

quality product?

18

SECTION II

Q. 7 Write short notes on Equilibrium solubility of Gases in

liquids for the following systems.

i. Two Component systems

ii. Multi Component systems

16

OR

Q. 8 A Write short notes on Reboiled absorbers. How efficiency

is increased in these type of absorbers?

8

B 5000Kg/hr of a So2 air mixture containing 5% by

volume So2 is to be scrubbed with 200000 Kg/hr of

water in a packed tower. The exit concentration of So2 is

reduced to 0.15%. The tower operates at 1 atm pressure.

The equilibrium relationship is given by Y=30X. If the

packed height of the tower is 420cm, estimate the height

of transfer unit.

8

Q. 9

1400Kg (bone dry) of granular solid is to be dried under

constant drying conditions from a moisture content of

0.2Kg/Kg dry solid to a final moisture content of

0.02Kg/Kg dry solid. The drying surface is given as

0.0615m2 /Kg dry solid. Under the same conditions the

following rates were previously know. Estimate the time

required for drying.

16

of 3

Moisture content 0.3 0.2 0.14 0.096 0.056 0.046 0.026 0.016

Rate of Drying 1.71 1.71 1.71 1.46 1.29 0.88 0.54 0.376

OR

Q. 10 Derive the expression for time of drying for the

following conditions.

i. Constant rate period

ii. Flling rate period

16

Q. 11 Give a brief description about the following

i. Methods of Super saturation

ii. Mier’s Super saturation theory

18

OR

Q. 12 A A hot solution containing 5000kg Na2CO3 and water

with a concentration of 25% by weight Na2CO3 is

cooled to 293 K and crystals of Na2CO3.10 H2o are

precipitated. At 293 K the solubility is 21.5kg anhydrous

Na2CO3per 100kg of water. Calculate the yield of

Na2CO3crystals obtained if 5% of the original water in

the system evaporates on cooling.

9

B Write short notes on solubility curves in Crystallization. 9

of 3

UNIVERSITY OF PUNE

[4363]-276

T. E. (BIOTECHNOLOGY), Examination 2013

REACTION ENGINEERING (315466)

(2008 Pattern)

[Total No. of Questions:12] [Total No. of Printed pages :3]


Instructions :

(1) Answers any 3 questions from each section

(2) Answers to the two Sections should be written in separate

answer-books

(3) Neat diagram must be drawn wherever necessary.

(4) Figures to the right indicate full marks.


SECTION I

Q1 a) Discuss about classification of chemical reactions. [6]

b) Define molecularity and order of reaction. [6]

c) Differentiate between elementary and non-elementary reactions with [6]

suitable examples.

OR

Q.2 a) Milk is pasteurized if it is heated to 600 C for 25 min but if it is heated [9]

to 700 C it only needs 15 sec for same process. Determine the activation

energy of sterilization process.

b) Explain kinetic model for nonelementary reaction. [9]

Q.3 a) A homogeneous gas reaction A=3R at 2000 C has the rate -rA =10

-2 [10]

CA1/2

(mol/ lit.sec). Find the space time needed for 80% conversion of

50% A and 50% inert feed to a plug flow reactor operating at 2000 C

and 4 atm if the Initial concentration is 0.0625 mol/lit.

of 3

b) Define space time and space velocity for flow reactor. [6]

OR

Q.4 a) Derive the equations relating time, initial concentration and conversion [8]

of an ideal batch reactor for constant density system.

b) For first order homogeneous gaseous reacting A= 2.5 R is carried [8]

out in an isothermal batch reach at 2 atm with 20% (mole) inserts

present and the volume increases by 60% in 20 min. In case of constant

volume reactor, determine the time required for pressure to reach 8 atm

if initial pressure is 5 atm, 2 atm of which consist of inerts.

Q.5 a) Derive the expressions for residence time distribution for pulse input [8]

method.

b) Write short note on segregated flow model. [8]

OR

Q.6 a) Write short note on dispersion model. [7]

b) A sample of tracer hytane at 320 k was injected as a pulse to a reactor [9]

and the effective conversion measured as a function of time, resulting

the following data,

t(min) 0 1 2 3 4 5 6 7 8 9 10 12 14

C(g/m3) 0 1 5 8 10 8 6 4 3 2.2 1.5 06 0

The measurement represents the exact concentration at the times listed and

not average values between the various sampling tests.

a) Construct a figure shows C (t) and E(t) as a function of time.

b) Determine both the fraction of material leaving the reactor that has spent

between 3 and 6 min. in the reactor.

of 3

SECTION II

Q.7 Write a short note on: [18]

i) Progressive Conversion Model ii) Shrinking Core Model

OR

Q.8 a) Derive overall rate equation for given system A(L) + B(S) =R(L). [9]

Dilute A diffuses through a stagnant liquid film onto a plane surface

consisting of B, reacts to produce R which diffuses back into the

mainstream. Develop the overall rate expression for the L/S reaction.

b) Explain diffusion through gas film controls in details. [9]

Q.9 a)Discuss about mechanism of solid catalysed reaction. [06]

b)Explain pore diffusion resistance combined with surface kinetics with

example [10]

OR

Q10 a) Determine the amount of catalyst required in packed bed reactor [8]

for 80% conversion of 1000 mol/min if CA0 = 8mol/m3 of feed.

b) Write short note on: [8]

i) Trickle bed reactor ii) Slurry reactor

Q11 a) Explain different types of inhibitors encountered in enzyme kinetics. [8]

b) Discuss about Michaelis-Menten kinetics. [8]

OR

Q12 Write short note on: [16]

i) Factors affecting growth kinetics

ii) Product limiting microbial fermentation

iii) Substrate limiting microbial fermentation

iv) Enzyme deactivation kinetics

of 4


UNIVERSITY OF PUNE

[4363]-278

T. E. (Biotechnology) Examination M a y - 2013

BIOSEPAREATIONS- I (315468) (2008 Course)


Instructions:

1 Answers to the two sections should be written in separate

answer-books.

2 Black figures to the right indicate full marks.

3 Your answer will be valued as a whole

4 Neat diagrams must be drawn wherever necessary.


SECTION –I

Q.1 A What is Ultrasonication ? Discuss various factors

affecting Ultrasonication.

6

B What is a Bioprocess? Give flow chart of major steps

involved in any bioprocess. Explain in detail

downstream processing of bioproducts.

10

OR

Q.2 A Explain types of biotechnology based on biocatalytic

developments.

6

of 4

B What are the different types of adsorption isotherms?

How these isotherms can be applied?

6

C Give importance of centrifugation in bioseparation

operations.

4

Q. 3 A Explain basis principle of Batch Chromatography?

What are the important parameters which affect elution

peaks of column chromatography?

9

B Write a short note on the following methods of

chromatography:

i) Frontal Analysis

ii) Elution Analysis

9

OR

Q. 4 A What is paper chromatography? Describe the working

procedure of paper chromatography with one example

6

B What is baseline separation? Explain in detail quality

criteria for ideal chromatographic separation

6

C A chromatographic separation of a two component

samples on a 60 cm column gave the retention of two

solutes X1 and X2 as 2.7 and 3.3 minute with base

widths of the two chromatographic peaks being 0.24

and 0.3 minutes respectively. Calculate :

i) No of theoretical plates

ii) Plate Height

iii) Resolution of two peaks

6

Q. 5 A What are the different techniques of centrifugation?

Explain in detail gradient centrifugation with

8

of 4

One example

B A laboratory centrifuge is used to collect yeast cells

after fermentation. The centrifuge consists of a number

of cylinders rotated perpendicular to the axis of

rotation . During centrifugation the distance between

the surface of liquid and the axis of rotation is R0 = 3

cm, and the distance form the bottom of the cylinder to

that axis is R = 10 cm. The yeast cells can assumed to

be spherical, with a diameter of d = 8 x 10-4

cm and a

density of 𝜌𝑠 = 1.05 g/cm3. The fluid has physical

properties close to those of pure water 𝜌 = 1.0 g/cm3, 𝜂

= 1.1 x 10-3

Pa.sec. The centrifuge is to be operated at a

speed of 1000rpm. How long does it take to have a

complete separation?

8

OR

Q. 6 A What is the difference between cross flow filtration and

dead end filtration? Explain with neat sketches which

type of filtration is more effective

8

B Give various types of membrane filtration modules.

Explain in detail spiral wound module with its

application

8

SECTION II

Q. 7 A Explain basic principle of solvent extraction. How

partition co-efficient plays important role in solvent

extraction processes?

8

B What is drying? Define terms bound moisture content

and unbound moisture content with one example

4

of 4

C Draw a neat sketch of drying rate curve and explain

how drying place in four stages.

6

OR

Q. 8 A Draw a neat sketch of saturation curve and explain

steps involved in crystallization process

8

B Write a short note on :

i) Lyophilizer

ii) Microwave assisted drying

10

Q. 9 A What is the scope of bioseparation techniques in

industries? Explain how it differs from scope in

research.

8

B What is scale up? Explain its importance in process

economics aspect by giving one detailed case study

8

OR

Q. 10 A Give detailed case study of following processes

I) Freeze drying of lactic acid bacteria

II) Citric acid production

16

Q. 11 A Explain with a neat sketch difference between primary

and secondary metabolites. Give detail note on

recovery of primary and secondary metabolites.

16

OR

Q. 12 A Give detail case study on separation of penicillin as a

secondary metabolite.

16

of 2


UNIVERSITY OF PUNE

[4363]-279

T. E. (BioTechnology) Examination - 2013

IMMUNOLOGY AND DIAGNOSTICS (2008 Course)


Instructions:

1 Answer Q1 or Q2, Q3 or Q4, Q5 or Q6 from Section I and Q7

or Q8, Q9 or Q10, Q11 or Q12 from Section II


answer-books.


SECTION -I

Q.1 Name various features of a secondary immune

response that distinguish it from a primary immune

response.

18

OR

Q.2 Describe the various specific defense mechanisms that

the immune system employs to combat various

pathogens.

18

Q. 3 Answer the following (8 marks each) 16

i What is antigenic specificity? What is an epitope?

What is a paratope?

ii Define cell mediated immunity and explain its

benefits.

OR


i What was Louis pasteur’s contribution in the field of

Immunology?

ii What is the inflammation? Explain the role in Immune

response

Q. 5 Explain (8 marks each) 16

i The principle and procedure for Sandwich ELISA

ii MHC role in immune response

of 2

OR

Q. 6 Write short notes (4 marks each) 16

i Different types of WBCs

ii Endogenous pathways of antigen presentation

iii Electrophoresis

iv Lattice hypothesis

SECTION II

Q. 7 Graft rejection is an immunologic response- Explain

giving the major types of rejection reactions.

18

OR

Q. 8 Explain the mechanisms involved in GVHD 18


i Diagrammatically represent the life cycle of HIV

ii Differentiate between normal cells and cancer cells

OR

Q. 10 Answer the following(8 marks each) 16

i What are DNA vaccines? Explain the advantages and

disadvantages.

ii Explain step-wise the classical pathway

Q. 11 Write short notes(4 marks each) 16

i Immunodeficiency disorders

ii Atopy

iii Immunological surveillance

iv Cancer vaccines and its importance

OR


i Define autoimmune diseases, giving 2 examples.

ii Explain- Immunological Principle of Vaccination

of 2


UNIVERSITY OF PUNE

[4363]-280

T. E. (Biotechnology) Examination - 2013

BIOINFORMATICS AND MANAGEMENT(2008 Course)


Instructions:

1 Answer 3 questions from Section I and 3 questions from

Section II.


answer-books.

3 Black figures to the right indicate full marks.



SECTION -I

Q.1 What is a database management system?Describe NCBI

as database of databases ,also enlist different applications

of bioinformatics.

18

OR

Q.2 Give an overview of various biological databases. 18

Q. 3 A Write a short note on specialized genomic resources like

Saccharomyces Genome Database (SGD),and Unigene.

8

B Describe NCBI data model. 8

OR

Q. 4 Describe the Sequence Retrieval System(SRS)and Entrez

, compare and contrast between them.

16

Q. 5 Write short note on (8 marks each)

(i)SWISS-PROT and

(ii)TrEMBL

16

of 2

OR

Q. 6 Describe briefly the various levels of protein structural

organization and comment on the significance of using it

as a basis for database organization.

16

SECTION II

Q. 7 Describe the following

(i)Pairwise and Multiple Sequence alignment 9

(ii)Local and Global Alignment 9

OR

Q. 8 Write briefly about BLAST and describe the significance

and use of its variants.

18

Q. 9 Write about the application of bioinformatics in drug

designing.What are the applications of Phylogenetic

analysis.

16

OR

Q. 10 Describe concept of phylogeny.What are the steps

involved in construction of a Phylogenetic tree, describe

briefly.Draw tree depicting homologs orthologs and

paralogs describe each.

16

Q. 11 What is concept of management and describe the basic

functions of an organization?Describe the various

management disciplines.

16

OR

Q. 12 Describe in detail the concept of “Technology Transfer”.

Describe SWOT analysis with the help of a case study.

16

university of pune [4363]-274 t. e. (biotechnology...

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