uottawa2k9 biosafety training pierre laflamme may 16, 2012 office of risk management, environmental...
TRANSCRIPT
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Uottawa2k9
BIOSAFETYTRAINING
Pierre Laflamme
May 16, 2012
Office of Risk Management,
Environmental Health and Safety
Human Resources - Occupational
Health Disability & Leave
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COURSE OUTLINE
Introduction Laboratory Associated Infections Blood-borne Pathogens Classification of Biohazards Infection/Biohazard Control Spill Response Biomedical Waste Regulations
BIOSAFETY
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INTRODUCTION
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What is a BIOHAZARD?
Any organism or its toxin that is known to cause disease in humans or animals or that is a potential hazard to humans, animals or the environment.
Examples:Microorganisms such as viruses, bacteria, fungi, and parasites and their toxins. Blood, body fluids and tissues from humans and animals.Transformed cell lines
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What is BIOSAFETY?
The combination of measures employed when handling biohazardous materials to: Protect personnel from exposure to infectious
agents Prevent environmental contamination Provide an environment for high quality
research while maintaining a safe work place Comply with applicable federal, provincial and
municipal requirements
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How is BIOSAFETY achieved?
Administrative controls Training, Inspections, Permits and Certificates
Engineering Controls Biological Safety Cabinets, Ventilation
Personal Protective Equipment Practices and Procedures Medical Surveillance Immunization when necessary
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What is BIOSECURITY?
Measures employed to protect biohazardous materials, or critical relevant information, against theft or diversion by those who intend to pursue intentional misuse.
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How is BIOSECURITY achieved?
Physical barriers Buildings, doors, locks, key card access
Psychological barriers Security personnel, cameras
Monitoring Activities Patrols, monitoring by support staff
Personnel Clearance Access to authorized personnel only
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Who are the STAKEHOLDERS?INTERNALLY Vice-President (Research) Committees University Services (ORM,
HR, PRS, PS) Deans, Chairs, Principal
Investigators Employees, Students Manager of Biological
Containment Suite
EXTERNALLY Public Health Agency of
Canada Canadian Food Inspection
Agency Environment Canada Transport Canada Ontario Ministry of Labour Emergency Response
Personnel Suppliers & Contractors Community
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University KEY SERVICES
Office of Risk Management, Environmental Health and Safety
Certificates and Permits
Training
Procedures (Waste disposal)
Risk Identification (Inspections)
Emergency plans
Accident/Incident follow-up
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University KEY SERVICES HR (Occupational Health, Disability and
Leave)Medical surveillanceImmunizationsMedical Follow-upInterface with Workplace Safety and Insurance
Board
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WHY ARE WE CONCERNED?
Potential for acquiring a laboratory-associated infection (LAI)
Contamination of the environment
Contamination of research
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LABORATORY ASSOCIATED INFECTIONS
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LABORATORY ASSOCIATED INFECTIONS
Route of Transmission
Susceptible Host
Infection Source
Percutaneous inoculation (needles and bites)
Inhalation of aerosols Contact of mucous membranes Ingestion
Microorganisms Cells and tissues Blood and body
fluids Any items
contaminated with the above
Immune system Vaccination status Age
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Only 20% of LAIs are related to a causative or defined event
80% are caused by human errors 20% are caused by equipment failure
Types of accidents causing LAIs Spills and sprays Needles Sharp objects and broken glass Bites or scratches from animals
http://www.weizmann.ac.il/safety/bio2.html
LAIs
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BLOOD-BORNE PATHOGENS
Human ResourcesOccupational Health Disability & Leave
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BLOODBORNE PATHOGENS (BBP) Sources
BloodSemenVaginal SecretionsOther Bodily Fluids:
CerebrospinalAmnioticSynovial
Tissue CulturesOrgan CulturesInfected Experimental
Animals
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RISK OF EXPOSURE
Pathogen involved Type of body fluid Route of exposure Duration of exposure Volume of blood involved in exposure Concentration of virus at time of
exposure PPE worn
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SPECIFIC EXAMPLES OF BBPS
Hepatitis B
Hepatitis C
HIV
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Pathogen Hepatitis B Hepatitis C HIV
Pathogenicity 2 major forms: • asymptomatic• symptomatic
• asymptomatic• symptomatic
• non-specific symptoms• acute infection: non-specific “flu-like”,
“mono-like” symptoms
Mode of transmission
• percutaneous/ permucosal exposure to body fluids, organs
• indirect contact with contaminated lab items (e.g. needles, syringes)
• Percutaneous exposure to contaminated blood (102 – 103 infectious particles/ mL)
• intravascular inoculation (e.g. transfusion) of contaminated blood products
• direct exposure of virus to mucosa (oral, rectal, vaginal)
Incubationperiod
• usually: 24 - 180 days• average: 60-90 days
• 2 weeks - 6 months• most commonly 7 – 10
weeks• chronic infection may
persist up to 20y before onset of cirrhosis
• variable• generally 1 - 3 months between time of
infection to development of detectable Ab’s• time from HIV infection to diagnosis of
AIDS ranges from < 1y to 15y or more
Survival outside host
• Survives in dried blood for long periods (weeks)
• stable on environmental services for at least 7 days at 25 °C
• not known• similar to hep B (survives
in dried blood for long periods…weeks)
• viable in blood in syringes @ RT for 42d• Cell-free HIV dried on glass coverslips in
10% serum can survive forlonger than 7d, depending on initial titre
Laboratory-acquired infections (LAIs)
• MOST FREQUENTLY occurring LAI
• lab workers incident rate: 7X > general population
• health care workers handling blood at higher risk to infection
• low (e.g. as of 2001, total of 57 cases of documented occupationally acquired HIV among US health care workers
FACTS ABOUT SOME BBPs
[Source: http://www.phac-aspc.gc.ca/lab-bio/res/psds-ftss/index-eng.php]
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ISSUES TO CONSIDER
Symptoms Mode of transmission Incubation period Survival outside host Communicability Immunization Prophylaxis / Treatment
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IF AN EXPOSURE OCCURS
Initiate first aid Notify your supervisor / designated
person Report to hospital emergency
department or University’s Health Services
Report incident to OHDL
Occupational Health, Disability and Leave Office, ext. 1472 http://www.rh.uottawa.ca/00_main/index_f.asp
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UNIVERSAL PRECAUTIONS Minimum standard of practice for
preventing the transmission of BBP includes:
EducationHand washingWearing protective barriersUse safe work practices
If samples cannot be guaranteed non-infective …… treat as infectious!
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BIOHAZARD CLASSIFICATION
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BIOHAZARD CLASSIFICATION Conventional Agents – Risk Groups 1 to 4 Recombinant DNA Tissue Culture Animal Work Anatomical Specimens Unconventional Agents
Class D, division 3 of WHMIS
(Poisonous and Infectious Material - Biohazardous Infectious Material)
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BIOHAZARD CLASSIFICATION Conventional agents are categorized into risk
groups based on their particular characteristics such as: Pathogenicity (Infectivity of the agent - disease,
severity, mortality) Infectious dose Mode of transmission (Airborne, Ingestion,
Parenteral) Host Range (Animal or human pathogen Availability of effective preventive measures
(PPE) Availability of effective treatment
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BIOHAZARD CLASSIFICATION
Conventional agents are categorized based on the measures required for handling each organism safely in a laboratory setting, such as: Operational Requirements (Protocols, Biological
safety cabinets, Lab safety practices) Engineering Requirements (Maintenance,
certification, repairs) Physical Requirements (PPE)
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Risk Group
Individual Risk
Community Risk
Containment
Level
Examples
1 Low Low Level 1 Escherichia Coli
2 Moderate Limited Level 2 Bacteria: Streptococcus and SalmonellaViruses: Adenovirus, Hepatitis A, B & C, Influenza
3 High Low Level 3 Bacteria: Bacillus anthracis and, Mycobacterium tuberculosisVirus: HIV
4 High High Level 4 Viruses: Ebola virus and Lassa virus
Unlikely to cause disease in healthy workers or animals
Rarely cause serious human or animal
disease
May cause serious disease
Likely to cause very serious disease
CONVENTIONAL AGENTS
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RECOMBINANT DNA
Recombinant DNA technology or genetic engineering:
in vitro incorporation of genetic material from one cell into another or from one organism to another
In Canada the level of risk depends on the source of DNA being transferred the vector the host
The Office of Risk Management will assist the investigator in this determination.
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TISSUE CULTURE
Mammalian cell lines have to be considered infectious as they may contain infectious agents
Untransformed mammalian cell lines - Risk Group 1 MCF-7 (Human breast carcinoma cell line) NIH 3T3 (Mouse fibroblast cell line)
Transformed mammalian cell lines – Risk Group 2 HeLa (Human - contains papovavirus)
All mammalian cell lines should be handled in a Level 2 Containment.
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ANIMAL WORK
Animals can harbour infectious agents (naturally or introduced) which can be transmitted to humans Scratches, bites, aerosols (needles and litter
changes), body fluids and excrements Level dependent on type of work being
conducted. Special Animal Care training is required for all
personnel working with animals. All work involving animal use must receive
prior approval from the Animal Care Committee
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All specimens should be considered infectious due to potential presence of infectious agents
It’s important to consider the type of specimen blood, organs, tissues Spinal sample, brain tissue From infectious patient
In general Level 2 but it depends on the nature of the work.
ANATOMICAL SPECIMENS
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UNCONVENTIONAL PATHOGENS
Includes unconventional agents, slow viruses and prions causing progressive neurological diseases Creutzfeld-Jakob disease in humans, Mad Cow Disease,
Scrapie in sheeps and goats
Resistant to destruction by chemical and physical procedures that normally inactivate viruses
Precautions: Handle tissues as Risk Group 2 or higher Handle formalin-fixed tissues and paraffin-embedded
blocks as if still infectious Follow up-to-date disinfection protocols.
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Earth Sciences
Earth Sciences
EngineeringCivil
Chemical
EngineeringCivil
Chemical
ChemistryChemistry Medicine/ Nursing
Medicine/ Nursing
HumanKinetics
HumanKinetics
Bio-EngineeringBio-Engineering
BiologyBiology
UniversityUniversity
WHERE ARE BIOHAZARDS FOUND?
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INFECTION/BIOHAZARD CONTROL
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INFECTION/BIOHAZARD CONTROL
1.Administrative Controls2.Engineering Controls3.Personal Protective
Equipment4.Practices and Procedures
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INFECTION/BIOHAZARD CONTROL
1. ADMINISTRATIVE CONTROLS
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ADMINISTRATIVE CONTROLS Administrative procedures to minimize the
risk of exposure: Risk assessment Training/Education Resources Inspections Permits and Certificates Medical Surveillance Signage
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ADMINISTRATIVE CONTROLSRisk Assessment Will determine for each biohazard:
Risk group Containment level Operational practices Safety measures
Responsibility of users Know and understand the various characteristics
of the agent(s) you are working with. (Material Safety Data Sheets and suppliers or
manufacturers information sheets)
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ADMINISTRATIVE CONTROLS Medical Surveillance Training & Education
WHMIS Lab specific policies and procedures Biosafety training, Laboratory safety training
Resources ORM web site, Biosafety page Faculty web sites Biosafety Manual Training Videos National and International Biosafety Guidelines
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ADMINISTRATIVE CONTROLS
Inspections Routine self-inspections Biosafety Inspection Checklist available
on-line In addition, ORM and Health, Safety
and Risk Officers will inspect labs to ensure compliance with regulations/ guidelines and provide feedback.
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ADMINISTRATIVE CONTROLS
Signs & Labeling Biohazard warning signs must be
posted on doors to rooms where biohazardous materials are used and/or stored.
Biohazard labels should be placed on containers, equipment and storage units used with biological agents.
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INFECTION/BIOHAZARD CONTROL
2. ENGINEERING CONTROLS
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ENGINEERING CONTROLS
Technology based Reduce or eliminate exposure to hazards Containment:
Types: Primary and Secondary Levels: 1, 2, 3 and 4
For effective containment, users must be aware of the potential hazards be trained Handle the material safely by adhering to
standard microbiological practices and techniques
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PRIMARY CONTAINMENT
First line of defence.
Ensures protection of personnel and immediate environment from exposure to the infectious agent.
‘Protective envelope’ that encapsulates the infectious agent or animal. Petri dish, vial Biological safety cabinets animal caging equipment
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SECONDARY CONTAINMENT
Protects the environment external to the laboratory from exposure
Includes facility design and operational practices
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CONTAINMENT LEVEL 1
Basic laboratory Requires no special design
features Biosafety cabinets are not
required and work may be performed on the open bench.
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CONTAINMENT LEVEL 2
Clinical, diagnostic, research and teaching facilities with level 2 agents.
Requires a class I or class II biological safety cabinet if any potential for aerosol or splash exists.
An emergency plan for handling spills must be developed.
Access should be controlled.
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Specialized design and construction with primary barriers to protect the individual secondary barriers to protect the environment
Requires type II or type III biosafety cabinets All staff must undergo specific training on the
agents used, PPE, equipment, waste management as well as practices and procedures.
CONTAINMENT LEVEL 3
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CONTAINMENT LEVEL 4
Only one level 4 facility in Canada (Canadian Centre for Human and Animal Health in Winnipeg, Man.)
Design specifications are extremely stringent The worker is completely isolated from
infectious material.
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BIOLOGICAL SAFETY CABINETS Primary containment Minimize contact between operator and the
infectious agent by the use of directional airflows
There are 3 main classes of cabinets (I, II, III) which provide various levels of protection.
Class II and III BSC contain HEPA filters which remove particles (min 0.3 microns) from supply and exhaust air with 99.97% efficiency .
BSC should be located away from doors and high traffic areas
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BIOLOGICAL SAFETY CABINETS
Laminar Flow Hoods or Clean Air BenchesVertical or horizontal laminar flowHEPA filtered supply air onlyProvide product protection onlyNot to be used with biohazards
Biological Safety CabinetLaminar air flow and HEPA filtered exhaust air Personnel and environment protectionHEPA filtered supply air & product protection with Class II + IIITo be used with biohazards
VS
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WORKING SAFELY IN A BSC
Step 1Before using the cabinet:
Turn off UV lamp; turn on fluorescent lamp Ensure BSC is certified Disinfect work surfaces with appropriate
disinfectant Place essential items inside cabinet Allow the blower to run for 5-10 min before
work
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Step 2While using the cabinet:
Ensure material and equipment is placed near the back of the hood, especially aerosol-generating equipment. Do not block any vents
Use techniques that reduce splatter and aerosols.
General work flow should be from clean to contaminated areas
Minimize movement so as not to impede air flow
WORKING SAFELY IN A BSC
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Step 3After using the cabinet:
Leave blower on at least 5 minutes to purge cabinet
Remove and decontaminate equipment and materials
Disinfect cabinet surfaces Turn off blower and fluorescent lamp, turn on
UV lamp
WORKING SAFELY IN A BSC
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Maintenance: Before and after each use - Wipe down work
surfaces Weekly - Clean UV lamp Monthly - Wipe down all vertical surfaces Annually - VerifyUV lamp intensity
- Decontamination with formaldehyde gas (by ORM)
- Certification (by ORM)
WORKING SAFELY IN A BSC
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INFECTION/BIOHAZARD CONTROL
3. PERSONAL PROTECTIVE EQUIPMENT
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PERSONAL PROTECTIVE EQUIPMENT
PPE is an important line of defence Responsibility of both the user and
the supervisor to ensure that PPE is worn
PPE is specific to each containmentlevel
Examples of PPE?
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PPE Criteria for consideration
Routes of exposure that need to be blocked
Degree of protection offeredEase of use
Only effective if correctly selected, fitted, used and cared
for the individual is well trained
Ensure PPE is removed before leaving the lab
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PPE
Footwear Closed toe and heel shoes
only. No sandals! Shoe coverings are worn in
some higher containment labs and animal facilities.
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PPE
Lab Coats/Gowns Protect street clothing from spills Offer additional body protection Long-sleeved, knee length with snaps Elastic cuffs Back-closing gowns Periodic cleaning required
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PPE
Gloves
Offer protection against a variety of hazards (heat, cold, chemical agents, biological agents, radioisotopes…)
Latex, nitrile, rubber & vinyl for work with biological agents.
Gloves should not be reused and should be changed frequently.
Utility gloves can be disinfected and reused if they show no sign of degradation.
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Eye & Face Protection Goggles, safety glasses to protect the eyes Full face shield to protect facial skin Offer protection against chemical and
biological splashes No contact lenses
Respirators Only personnel who have been fit-tested and
trained should wear respirators. Worn in atmospheres that pose an infectious
or toxic hazard
PPE
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MOVIE
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BREAK
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INFECTION/BIOHAZARD CONTROL
4. PRACTICES AND PROCEDURES
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PRACTICES AND PROCEDURES General Safety Guidelines Good Microbiological Practice Handwashing Receipt of Packages Opening Packages Specific Procedures
Centrifuges Needles & Syringes and other sharps Pipettes Blenders, Grinders, Sonicators & Lyophilizers Inoculation Loops Cryostats
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GENERAL LABORATORY SAFETY GUIDELINES
Understand the hazards you face in the laboratory Be adequately trained Appropriate PPE must be worn The lab should be kept clean and in order Long hair must be tied back Work surfaces must be cleaned and
decontaminated daily The use of needles should be limited Lab doors have to be closed Access to the lab has to be restricted
b i o s a f e t y
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1. GOOD MICROBIOLOGICAL PRACTICE (GMP)
Mitigates the risk of: 1. Personnel exposure2. Contamination
a) sample b) environment
What are we talking about ?
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2. GOOD MICROBIOLOGICAL PRACTICE (GMP)
Universal Precautions:• More knowledge about the organism being
used = easier to take the necessary precautions
• Appropriate PPE greatly minimizes risk of exposure
• Engineered controls (BSC’s) prevent release of aerosols outside cabinet (and helps protect user!)
• Frequent hand washing = avoid infections
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3. GOOD MICROBIOLOGICAL PRACTICE (GMP)
• Prepare yourself for the work:o Know what you will be doing o Structure the work in a logical fashion (work
flow)• Prepare the work area• Ensure all material that needs to be in the BSC is
sterile before placing it there • Ensure waste containers are at hand’s reach and
are not overflowing and likely to collapse/ fall over• Use aseptic technique• Consult web [http://www.protocol-online.org] for
SOPs & techniques• Properly trained to use equipment accordingly and
when in doubt…ASK!• Clean up and decontaminate
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4. GOOD MICROBIOLOGICAL PRACTICE (GMP)
• Disinfect work surfaces with suitable disinfectant before and after
• Clean spills immediately and disinfect area thoroughly
• Keep bench top uncluttered
• Minimize traffic and unnecessary movements around work area
• all work with infectious material should be carried out in a
specific area
• Material should not be carried throughout, or out of lab, unless in
a closed or capped container
• Minimize aerosol generation; if unavoidable, carry out activities in
a BSC
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5. GOOD MICROBIOLOGICAL PRACTICE (GMP)
• Keep sterile and non-sterile objects separate
• Minimize exposure to outside air
• Avoid contact with non-sterile surfaces and items
• Hold open containers at an angle whenever possible
• Identify and properly dispose of different types of
waste
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HANDWASHING
One of the single effective means of preventing infections if done properly and frequently
When to wash hands? Before starting any manipulations Before leaving the lab When hands are obviously soiled Before and after completing any task in a BSC Every time gloves are removed Before contact with one’s face or mouth At the end of the day
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RECEIPT OF PACKAGES
At Shipping & Receiving Verify shipment is yours, and expected. Inspect the integrity of container. If damage and breakage possible, transfer the package
into a secondary container lined with absorbent paper (absorbent side up)
Transfer to a cart with 4 sides for transfer to lab. Decontaminate all the areas in S&R where the package
came into contact with. All individuals who may have come into contact with the
material must wash their hands
REMEMBER AT THIS POINT YOU DO NOT KNOW IF THE SAMPLE HAS BEEN BE BREACHED !
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OPENING PACKAGES – IN LAB
Scenario 1: Package appears damaged.
• Transfer the sample to a biological cabinet and open and inspect each layer of packaging confronted with for signs which would indicate the sample integrity.
• If damaged, inform your supervisor and ORM (x. 3153)
• Dispose of sample in the appropriate manner
• Package must be sterilized or sent for incineration.
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OPENING PACKAGES – IN LAB
Scenario 2: Package is intact.
• Open package in the containment level required by the sample
• Add sample to inventory • Read and file MSDS or supplier information
sheet• Deface all markings on the package prior to
disposal
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SAFE USE OF CENTRIFUGES
Before use Check centrifuge tubes for cracks Avoid Overfilling Place caps or stoppers properly Balance loads Use sealed buckets (safety cups) or sealed rotors
Before leaving: ensure centrifuge achieves run conditions After run
Centrifuge has to be completely stopped before opening the lid Check for spills or leaks before removing samples. Clean spills Allow aerosols to settle (30 min) or open in a BSC
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NEEDLES AND SYRINGES
Avoid use whenever possible Use a BSC for all operations with infectious
material Fill syringes carefully Shield needles when withdrawing from
stoppers Do not bend, shear or recap needles. Dispose of all used needles/syringes in yellow
sharps containers
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PIPETTES
Mouth pipetting is prohibited. Never force fluids out. To avoid splashes, discharge the liquid down
the receiving container wall. Never mix material by suction and expulsion. Reusable pipettes should be placed
horizontally in a disinfectant filled pan.
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BLENDERS, GRINDERS, SONICATORS, AND LYOPHILIZERS
• Operate in a BSC whenever possible. Allow aerosols to settle for 5 minutes before opening.
• Decontaminate after use• Blender
Do not use glass blender jars Use safety blenders which can be autoclave
• Lyophilizers (used for dehydration process) Use glassware designed for vacuum work,
ensure there is no damage before using Use vapour traps whenever possible
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INOCULATION LOOPS
Sterilization in an open flame may create aerosols which may contain viable microorganisms.
Shorter handles minimize vibrations
Disposable plastic loops are good alternatives
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CRYOSTATS
Wear gloves during preparation of frozen sections and heavy gloves when accessing the cryostat.
Decontaminate frequently (70% Ethanol)
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SPILL RESPONSE
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SPILLS
Spill response will vary depending on: What was spilled? How much was spilled? Where was the spill? What is the potential for release to the
environment?
Spills should be cleaned up immediately (unless an aerosol was generated), to ensure proper decontamination.
Ensure appropriate PPE is worn and clean-up equipment is readily available.
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SPILLS-GENERAL CLEAN-UP
Cover spill area with absorbent material Soak the spill area with an appropriate disinfectant (i.e.
10% bleach) Pour disinfectant from the outside of the absorbent
material towards the inside Leave on for 20 to 30 minutes Pick up any broken glass (with forceps!) and place in a
sharps container Wipe up with absorbent material Waste should be disposed in appropriate biohazardous
waste container
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SPILLS-SPECIAL CASES
Within a Centrifuge Within a BSC Open Areas (lab, during transport) The spill response plan template is
available at (http://www.uottawa.ca/services/ehss/docs/SPILLRESPONSEPLAN.pdf)
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SPILLS All users of biological materials should be
familiar with the spill clean-up procedures.
All spills are to be reported ASAP to the lab supervisor and ORM.
Additional assistance is available from:
ORM x 5892
Your departmental safety officer
ERT x 5411 (through Protection Services)
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BIOMEDICAL WASTE
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DECONTAMINATION, DISINFECTION, AND STERILIZATION Decontamination: The destruction of
microorganisms to a lower level such that it removes danger of infection to individuals.
Sterilization: The complete destruction of all viable microorganisms.
Disinfection: Use of agents (physical or chemical) to destroy harmful organisms on inanimate objects
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DECONTAMINATION: PHYSICAL
Heat:Autoclaving (most practical and
recommended)Incineration (for disposal of sharps and
tissues) Irradiation:
UV light (wavelength of 253 nm is germicidal)
Gamma (disrupts DNA and RNA) Filtration
HEPA (biological safety cabinets, ventilation)
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AUTOCLAVES
Items that CAN be autoclaved: Cultures and stocks of infectious material Culture dishes and related devices Discarded live and attenuated vaccines Contaminated solid items (petri dishes,
eppendorf tips, pipettes, gloves, paper towels)
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AUTOCLAVES
Items that CANNOT be autoclaved: chemicals (flammables, oxidizers,
phenols, acids, alkalides) chemotherapeutic or radioactive waste bleach (or other chlorinated products) certain kinds of plastics Sharps (not at the University of
Ottawa)
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AUTOCLAVES
Preparation of waste: Use only approved autoclave bags Do not overfill autoclave bags Separate material for re-use from that which
will be disposed, and dry from liquid material If outside of bag is contaminated, double bag All flasks containing biological material should
be capped with aluminum foil Ensure items are labeled with contact
information
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SAFE USE OF AUTOCLAVES
Many autoclaves are now run by dedicated staff, however, if you are operating an autoclave: Learn how to use it! Ensure PPE is worn Recognize acceptable material and packaging Proper loading and unloading
All users/operators must take the autoclave training
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DISINFECTION: CHEMICAL
Generally for disinfection rather than sterilization
Choice depends on: Type of material to be disinfected Organic load Chemical characteristics
Most common are chlorine compounds and alcohols (broad range)
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WHAT TO USE FOR MY AGENT?
Vegetative bacteria (E.coli, Staph)
2% domestic bleach 75% Ethanol Quaternary ammonia 6% formulated Hydrogen peroxide
Mycobacteria and fungi
10% domestic bleach 75% Ethanol Phenolic compounds 6% formulated Hydrogen peroxide
Spore forming bacteria (Bacillus)
10% domestic bleach Gluteraldehyde Formaldehyde 6% formulated Hydrogen peroxide
VirusesEnveloped (HIV, Herpes)
2% domestic bleach 75% Ethanol Quaternary ammonia 6% formulated Hydrogen peroxide*
Non enveloped (Hepatitis, Adenovirus)
10% domestic bleach 6% formulated Hydrogen peroxide* Gluteraldehyde Formaldehyde
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WASTE MANAGEMENT
Discarded biological material from teaching, clinical
and research laboratories and operations is biomedical
waste.
Biomedical waste includes but is not limited to: Animal waste Biological laboratory waste Human anatomical waste Human blood and body fluid waste Sharps
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WASTE MANAGEMENT All biological waste should be decontaminated
prior to disposal (including level 1 agents).
Treated waste is no longer considered ‘biomedical’ (i.e. microbiological waste, blood and bodily fluid waste) and can be disposed of in the regular waste stream.
Any waste that cannot be treated (i.e. sharps, carcasses, tissues and body parts) remains biomedical waste and must be incinerated off site.
LABEL YOUR WASTE IDENTIFY CONTENTS
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WASTE DISPOSALBiomedical Waste (untreated)
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WASTE DISPOSALBiomedical Waste (treated)
with H2O (1:10)
*in compliance with
sewer use by-laws
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REGULATIONS
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KEY REGULATED ACTIVITIES
Purchasing & Receiving of Biological Agents
PHAC, CFIA, Environment Canada
Inventory Records Transportation/Transfer
Transport Canada- TDG All Agencies (provincial and federal)
emphasize and expect Biosecurity
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PURCHASING
Importation permits required by Public Health Agency Canada (PHAC) or Canadaian Food Inspection Agency (CFIA) for certain agents
Material Transfer Agreements (MTAs) between importer & exporter
US restrictions Ensure you meet all criteria and have
all pertinent documentation
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BIOMATERIAL ACQUISITION/ MTAs
“How soon do you need it?” “You want it when?”
In order to facilitate a quick turnaround, provide ORM with:
copies of MSDS’s references (hardcopies) as much background information re:
product as possible
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INVENTORY What material is presently being
used and/or stored LocationExpiry dateUse log book for remaining
amount MSDS’s
Mandatory
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SHIPPING AND RECEIVING
Transportation of Dangerous Goods Act: Class 6.2 (Infectious Substances)
PHAC/CFIA restrictions Ensure:
Proper classificationProper packagingProper labelingProper documentation Import/Export Permits
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TRANSPORTATION OF DANGEROUS GOODS
Pre-approved Authorized Individuals Lead time (International Regulations….) Appropriate Scheduling (Holidays,
Weekends) Transportation within the building Between lab to lab Colleague to Colleague Between Institutions
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TRANSPORTATION Important Considerations:
does material need to be transported at allpackaging requirements means and route of transportation regulatory requirements
Between lab transfers - 4 sided cart, sealed primary container, secondary container, low traffic route.
Off Campus transfers – consult ORM
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THE BOTTOM LINE
If you are not careful and diligent with biological agents you risk:Infecting yourself, others or the
environmentContaminating your researchHaving Public Health Agency of Canada,
Canadian Food Inspection Agency, Ministry of the Environment or Transport Canada after you
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BIOSAFETY WEBSITEhttp://www.uottawa.ca/services/ehss.biosafety.htm
Biohazardous Materials User Registration
Practical Training Form
Biosafety Health Assessment Survey
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MOVIE