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Urine collection in Neonates
CHHS16/235
Canberra Hospital and Health Services
Clinical Procedure
Urine Collection in Neonates
Contents
Contents1
Purpose2
Alerts2
Scope2
Section 1 – Bladder Tap3
Section 2 – Bag Collection of Urine4
Section 3 – Collection of Urine using cotton wool balls4
Section 4 – Collection of urine via urethral catheterisation5
Implementation6
Related Policies, Procedures, Guidelines and Legislation6
References7
Definition of Terms7
Search Terms7
Purpose
The purpose of this procedure is to obtain a sterile or non sterile urine specimen for testing for:
Bladder tap is performed to obtain sterile urine for culture
Bag urine can be collected for culture and sensitivity, metabolic screening, amino acid testing
Clinitest strip testing of urine – Ph, glucose, ketones, protein, blood, leucocytes
Catheterisation is performed for urinary retention in the neonate. This occurs most commonly with a muscle relaxed baby or congenital abnormalities affecting the bladder and urethra emptying ability (neurogenic bladder )e.g. myelomeningocele
This Standard Operating Procedure (SOP) describes for staff the process to
Scope
Alerts
Hand Hygiene: healthcare workers will comply with ACT Health hand hygiene practices in order to support the reduction of infection caused by the transmission of harmful pathogens.
Patient Identification Pathology Specimen labelling: defines the correct process that Health Directorate staff must comply with when identifying a patient and accurately labelling Pathology specimens collected from that patient.
Specimens must be received in pathology within two hours of collection
Rare Complications include bladder wall haematoma, lacerated vessel on anterior bladder wall, perforation of hollow viscus and osteomyelitis of pubic bone or abdominal wall abscess
Do not insert extra tubing length when inserting a urethral urinary catheter this will increase the risk of trauma and knotting
Do not use a catheter with a balloon and/or a guidewire
Scope
This document pertains to infants and babies nursed in the Neonatal Intensive Care (NICU) and Special Care Nursery (SCN)
This document applies to:
Medical Officers
Nurses and Midwives who are working within their scope of practice (Refer to Scope of Practice for Nurses and Midwives Policy)
Student Nurses and Midwives under direct supervision
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Section 1 – Bladder Tap
Equipment
Alcohol based hand rub (ABHR)
Basic set
25 gauge needle
5ml or 10ml syringe
Skin preparation (non alcohol)
Sterile specimen container
Patient identification label and labelled pathology form
Bandaid spot
Sucrose
1ml syringe
Procedure
1. Attend hand hygiene before touching the patient by either hand washing or using ABHR
2. Palpate bladder for fullness of bladder
3. Collect and prepare equipment
4. Position baby supine on flat surface and remove nappy
5. Administer sucrose 2 minutes prior to procedure for pain relief according to policy for Sucrose for pain relief
6. Immobilise baby by gently holding ankles and arms. Firmly wrap upper body
7. Medical officer is to scrub hands and don sterile gloves
8. Medical officer to prepare pubic area which is swabbed 3 times
9. Medical officer - 25-gauge needle attached to a 3-ml syringe is directed perpendicular to the skin just superior (0.5 cm) to the symphysis in the midline and advanced to its hub
Fig 1. Technique for supra pubic bladder aspiration (2)
10. The needle is withdrawn, slowly, with slight pressure pulling back on the syringe
11. A minimal amount of haematuria may be seen after an attempt, but otherwise the risks are minimal
12. Ultrasound may be used to ascertain bladder fullness prior to bladder tap
13. Apply bandaid to puncture site
14. Place the urine in the specimen jar
15. Label specimen container and send to pathology with request form
16. Settle neonate comfortably
17. Record in progress notes and observation chart
18. Dispose of equipment according to OH&S standards
19. Document the procedure on the observation chart, problem sheet and in the progress notes
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Section 2 – Bag Collection of Urine
Equipment
Sterile specimen container
Basic set
5ml or 10ml syringe
Skin preparation (non alcohol)
Sterile urine bag
Procedure
1. Place infant in supine position and remove nappy
2. Clean and dry thoroughly genital area
3. Carefully open the sterile urine bag without contaminating the bag
4. Apply the bag by attaching the bottom of the bag to the peritoneum or scrotal area
5. Once the baby has voided remove the urine bag by using no sting barrier wipe
6. Drain the urine from the bag by removing blue tab and place in sterile container
7. Reapply nappy, wash hands and settle baby
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Section 3 – Collection of Urine using cotton wool balls
Equipment
sterile cotton wool ball
10 ml syringe
sterile specimen collection container
clinitest strip
Procedure
Collection of urine using cotton wool balls:
1. place clean cotton wall ball inside baby’s nappy closest to urethral opening for best absorption of urine into cotton wool ball
2. remove moist cotton wool ball from the baby’s nappy with the next cares
3. place cotton wool ball in to the hub of the syringe. Press syringe head into hub to squeeze out urine on to Clinitest strip
4. document findings of Clinitest urine test on patient flow chart care plan and in the patents clinical notes
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Section 4 – Collection of urine via urethral catheterisation
Equipment
Sterile dressing pack
Gauze squares - extra
Chlorhexidine 0.1% (warmed)
5fg feeding tube (always have a spare feeding tube)orappropriately sized indwelling catheter
Lubricant- sterile water or KY jelly
Sterile yellow top jar
Sterile scissors
Sterile gloves
Steri strips
Another staff member to assist with holding
Sucrose
Sterile fenestrated green drape
Procedure
1. Inform parents of the procedure
2. Sucrose for pain relief
3. Open all equipment using asceptic technique
4. Place baby in the supine position with legs abducted
5. Hand hygiene as per the ‘Asceptic Non Touch Technique’ policy
6. Don sterile gloves
7. For a male baby stabilise the shaft of the penis with non-dominant hand, perpendicular to the body. This hand is now considered contaminated.
· apply gentle pressure at the base of the penis to avoid reflex urination
· clean the penis with antiseptic solution starting at meatus and moving down the shaft of the penis. Allow the antiseptic to dry
· gently insert the catheter into the meatus until urine is seen in the catheter sterile lubricant to catheter tip. Never force the cather
· If the catheter is to remain indwelling, immediately connect the catheter to closed urinary collection system (yellow topped jar or urine bag)
· secure with Steri Strips to the baby’s inner thigh
8. Female catheterisation
· with the non-dominant hand separate the labia using sterile gauze
· using the free hand for the rest of the procedure, clean the area around the meatus with antiseptic solution using anterior-to-posterior strokes to prevent drawing faecal material in to the field. Allow the antiseptic to dry
· apply sterile lubricant or sterile water to catheter tip for easier inserton
· gently insert catheter until urine is visible in catheter tubing. Do not insert extra tubing
· if catheter is accidentally inserted into vagina, leave in place and insert new catheter anterior to the first catheter
· connect to closed urinary collection system and secure
9. Send urine sample for culture if required
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Implementation
Implementation via:
training for all staff in NICU and SCN during orientation
Access to SharePoint for information on Policies, Procedures and Guidelines
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Related Policies, Procedures, Guidelines and Legislation
Policies Healthcare Associated Infections ProceduresPatient Identification-pathology specimen labelling
Patient identification and procedure matching policy
Pathology requests and specimens
Urine specimen management procedure
Urine collection via bladder tap or sterile bag
Waste Management Policy
Asceptic Non Touch Technique
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References
1. Hoppert M. Microscopic techniques in biotechnology. Weinheim: Wiley-VCH; 2003.
2. Drummond PD. Triggers of motion sickness in migraine sufferers. Headache. 2005;45(6):653-6.
3. Meltzer PS, Kallioniemi A, Trent JM. Chromosome alterations in human solid tumors. In: Vogelstein B, Kinzler KW, editors. The genetic basis of human cancer. New York: McGraw-Hill; 2002. p. 93-113.
4. Reliability of Reagent Strips for Semi-quantitative Measurement of Glucosuria in a Neonatal Intensive Care Setting Jolita Bekhofa, Boudewijn J. Kollenb, Sjef van de Leurc, Joke H. Kokd,Irma H.L.M. van Straatena
5. Austin, BJ, Bollard C, Gunn T. Is urethral catheterization a successful alternative to suprapubic aspiration in neonates? Journal of Paediatrics and Child Health, Issue 1, Feb 1999,p 34-36
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Definition of Terms
Glycosuria – Urine testing positive for glucose on Clinitest urine testing
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Search Terms
Urine, urine collection, urine bag, bladder tap, Clinitest testing
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Disclaimer: This document has been developed by ACT Health, Canberra Hospital and Health Service specifically for its own use. Use of this document and any reliance on the information contained therein by any third party is at his or her own risk and Health Directorate assumes no responsibility whatsoever
(to be completed by the HCID Policy Team)
Date Amended
Section Amended
Approved By
Eg: 17 August 2014
Section 1
ED/CHHSPC Chair
Doc Number
Version
Issued
Review Date
Area Responsible
Page
CHHS16/245
1
15/12/2016
01/07/2021
WY&C
1 of 7
Do not refer to a paper based copy of this policy document. The most current version can be found on the ACT Health Policy Register