UriSelect™4 MediumDirect Identification Visibly Reliable

Infectious Disease Testing

Non selective chromogenic medium for the isolation, differentiation and enumeration of urinary tract infections pathogens within 18-24 hours:■ Isolation and identification of both Gram-positive and Gram-negative pathogens in a single plate

■ Chromogenic mix allowing the detection of specific enzymes activities

■ Direct identification of E. coli, Enterococcus spp. and Proteus mirabilis via revelation and visualization of enzymatic activity of bacteria

■ Presumptive identification of KESC, Streptococci and Staphylococci (diffentiation of S. saprophyticus)

■ Inhibition of Proteus spp. swarming with an original formulation

UriSelect™ 4

Urine specimen inoculation with a 10 μL calibrated loop

Incubation 18-24 hrs at 35-37°C

Enumeration:Compare the density of colonies present on the plates, with those illustrated below:

Assay Procedure

106 CFU/mL 105 CFU/mL 104 CFU/mL 103 CFU/mL

Proteus mirabilis 24 hrs

Interpretation of bacteriuria: Counting of well-isolated colonies on UriSelect™ 4 should be possible up to 104 CFU/mL

of urine. Above that concentration, presence of confluent colonies does not result in accurate counts.

E. coli24 hrs

SS

SS

Direct Identification of E. coli, Proteus mirabilis and Enterococcus spp.

Presumptive Identification of KESC

Citrobacter koseri

Serratia marcescens

Klebsiella pneumoniae

Enterobacter cloacae

Proteus mirabilis

Orange-brown colonies with possible brownish halo:

TDA , Indole

Enterococcus spp.

Turquoise-blue colonies: β-Glucosidase

Escherichia coli

Pink colonies: β-Galactosidase

Indole

Pseudomonas aeruginosa

Staphylococcus epidermidis

Streptococcus agalactiae

Staphylococcus aureus

Examples of Other Pathogens Growth

Acinetobacter baumanii

Candida albicans

Staphylococcus saprophyticus

* Klebsiella, Enterobacter, Serratia and Citrobacter

** Tryptophan Desaminase

S

Complete the identification using a conventional procedure

Observation of the color of colonies:

S S

S

Examine undermicroscope

Gram ⊝ Bacillivery likely

KESC *

SS

Blue-purple colonies= β-Galactosidase ⊕

and β-Glucosidase ⊕

18 hrs mixed culture of Klebsiella spp. and Enterococcus spp.

18 hrs mixed culture of E. coli, Enterococcus spp. and

Proteus spp.

Visualization and Identification of Mixed Cultures

Pathogens Identification

S

S S S

SS SS

SS

Pink colonies= β-Galactosidase ⊕

Turquoise blue colonies

= β-Glucosidase ⊕

Orange-brown colonies with a possible brownish halo

= TDA **⊕

Examine undermicroscope

Test for indoledirectly on a colony

Test for indoledirectly on a colony

Gram ⊕ CocciEnterococci

⊝ Proteusmirabilis

⊕ E.coli

⊕ Proteus spp.,

Morganella spp.,Providencia spp.

⊝

S S

S

White, other color or colorless colonies

Contrasting colors of colonies enhanced by a pearl-grey opaque background allow for an optimal visualization of polymicrobial mixtures.

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Clinical Diagnostics Group

© Bio-Rad 2016/02Printed in France with soy-based inks on paper from sustainably managed forestsBulletin 16364 Rev D

Bio-RadLaboratories

Catalog # Description

Rapid Detection of Resistance to Third Generation Cephalosporins for Enterobacteriaceae68250 b LACTA™ Test, 50 tests

Rapid Detection of Carbapenemase-Producing Enterobacteriaceae68260 b CARBA Test, 25 tests

Rapid Identification of Carbapenemases TypesK-15R1 OXA-48 K-SeT*K-15R2 KPC K-SeT*

* Manufactured by and distributed by Bio-Rad.

Ask for availability in your country.

Other Chromogenic Media63757 MRSASelect™II, 20 plates x 90 mm63748 SASelect™, 20 plates x 90 mm63751 VRESelect™, 20 plates x 90 mm63750 StrepBSelect™, 20 plates x 90 mm63740 CandiSelect™, 20 plates x 90 mm

Ordering InformationCatalog # Description

Urinary Tract Infections63726 UriSelect™4, 20 plates x 90 mm63727 UriSelect™4, 100 plates x 90 mm64694 UriSelect™4 (dehydrated), 500 g

55313 Kovacs reagent, 2 vials x 15 ml63713 Urea Indole medium, 50 ampules x 1 ml53834 O.X. (Oxidase), 2 x 50 disks56350 Novobiocin (5 µg), 30 disks56356 Pastorex™ Staph Plus, 50 tests56353 Pastorex™ Staph Plus, 5 x 50 tests61721 Pastorex™ Strep, 60 tests56513 AuxaColor™ 2 kit, 20 tests

Visit www.bio-rad.com/select-chromogenicmedia for more information.

VRESelect ™

UriSelect ™4

MRSASelect™ II

CandiSelect ™

StrepBSelect ™

SASelect ™

Chromogenic Media Range