use of hptlc, hplc, and densitometry for qualitative separation of indole alkaloids from rauvolfia...
DESCRIPTION
High-performance thin-layer chromatography (HPTLC) has been used for normal-phase separation of the components of hexane, chloroform, methanol, and water extracts of Rauvolfia serpentina roots. Computerized densitometry was used for two-dimensional spectrographic image analysis of the HPTLC plates. High performance liquid chromatography (HPLC) was also used for reversed-phase separation of these extracts. Different chromatograms of R. serpentina root extracts, obtained by use of these techniques, revealed the presence of three marker indole alkaloids, ajmaline, ajmalicine, and reserpine, in all four extracts. Use of chloroform resulted in most efficient extraction of these three alkaloids. The results also showed that defatting with hexane may result in loss of the alkaloids.TRANSCRIPT
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Journal Article
Use of HPTLC, HPLC, and densitometry for qualitative separation of indole alkaloids from Rauvolfia serpentina roots Journal JPC - Journal of Planar
Chromatography - Modern TLC
Publisher Akadémiai Kiadó
ISSN 0933-4173 (Print) 1789-0993 (Online)
Issue Volume 19, Number 110/August 2006
Category Original Research Papers
DOI 10.1556/JPC.19.2006.4.5
Pages 282-287
Online Date Wednesday, March 07, 2007
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Authors
Madan Mohan Gupta1, Alpana Srivastava1, Arvind Kumar Tripathi1, Himanshu Misra1, Ram Kishore Verma1
1Central Institute of Medicinal and Aromatic Plants Analytical Chemistry Division P.O. CIMAP Lucknow 226015 India
Abstract
High-performance thin-layer chromatography (HPTLC) has been used for normal-phase separation of the components of hexane, chloroform, methanol, and water extracts of Rauvolfia serpentina roots. Computerized densitometry was used for two-dimensional spectrographic image analysis of the HPTLC plates. High performance liquid chromatography (HPLC) was also used for reversed-phase separation of these extracts. Different chromatograms of R. serpentina root extracts, obtained by use of these techniques, revealed the presence of three marker indole alkaloids, ajmaline, ajmalicine, and reserpine, in all four extracts. Use of chloroform resulted in most efficient extraction of these three alkaloids. The results also showed that defatting with hexane may result in loss of the alkaloids.
Keywords
HPTLC, HPLC, Densitometry, Rauvolfia serpentina, Alkaloids
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H.J. Pfeifer, D.J. Greenblatt , and J. Koch-Weser , Am. J. Med. Sci. 271 (1976) 269–276.
M.J. Ellenhorn and D.G. Barceloux , Medical Toxicology — Diagnosis and Treatment of Human Poisoning, Elsevier, New York, 1988, pp. 295–299.
A.G. Gilman, T.W. Rall, A.S. Nies , and P. Taylor (Eds) Goodman and Gilman’s The pharmacologic Basis of Therapeutics, 8th edn, Pergamon Press, New York, 1990, pp. 795.
G.G. Briggs, R.K. Freeman , and S.J. Yaffe (Eds) Drugs in Pregnancy and Lactation — A Reference Guide to Fetal and Neonatal Risk, 2nd edn, Williams and Wilkins, Baltimore, MD, 1986, pp. 402/r–403/r.
C.V. Poser, H.H. Andrade, K.V. Silva da, A.T. Henriques , and J.A. Henriques , Mutat. Res. 232 (1990) 37–43.
H.D. Neuwinger , African Ethnobotany — Poisons and Drugs, Chapman and Hall, New York, 1996, pp. 941.
H.N. Nigg and D.S. Seigler (Eds) Phytochemical Resources for Medicine and Agriculture, Plenum Press, New York, 1992, p. 445.
O.P. Virmani, S.P. Popli, L.N. Misra, M.M. Gupta, G.N. Srivastava, Z. Abraham , and A.K. Singh , Dictionary of Indian Medicinal Plants, CIMAP, Lucknow, India, 1992, p. 387.
J.D. Phillipson and M.H. Zenk , Indole and Biogenetically Related Alkaloids, Academic Press, London, 1980, pp. 298.
J.B. Harborne and H. Baxter (Eds) Phytochemical Dictionary. A Handbook of Bioactive Compounds from Plants, Taylor and Frost, London, 1983, pp. 791.
W. E. Court , Can. J. Pharm. Sci. 1 (1966) 76–79.
M. Petkovic , Arh. Farm. 20 (1970) 173–175.
M.S. Habib and W.E. Court , J. Pharm. Pharmacol. 23 (Suppl.) (1971) 230S.
F. Hammerstein and F. Kaiser , Planta Med. 21 (1972) 5–25.
K.C. Ngugen and I.G. Nikalova , Rastit. Resur. 25 (1989) 594–599.
A.G. Vollosovich, I.R. Moleva , and E.S. Karbelainen , Rastit. Resur. 30 (1994) 143–147.
U.R. Cieri , J. Assoc. Off. Anal. Chem. 66 (1983) 867–873.
U.R. Cieri , J. Assoc. Off. Anal. Chem. 70 (1987) 540–546.
V.Ye. Klyushnichenku, S.A. Yakimov, T.P. Bychkova, Ya.V. Syagailo, I.N. Kuzovkina, A.N. Bulfson , and A.I. Miroshnikov , Khim. Farm. Zh. 28 (1994) 58–61.
A. Srivastava, H. Misra, R.K. Verma , and M.M. Gupta , Phytochem. Anal. 15 (2004) 280–285.
M. Gu, F. Ouyang , and Z. Su , J. Chromatogr. A 1022 (2004) 139–144.
K. Koll, E. Reich, A. Blatter , and M. Veit , J. AOAC Int. 86 (2003) 909–915.
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