Use of the Labnet Micropipettor

Purpose of the Pipettor

• Biotechnologists use a pipettor to transfer precise amounts of liquids from one container to another.

• The measurements are in microliters 1 ml=1000 µl.

Use of the Labnet Micropipettor

Care of the Pipettor

• Misuse of the pipettor can result in incorrect measurements affecting your investigation.

• Do not drop the pipettor.• Do not adjust the volume

to less than 2 µl.

Use of the Labnet Micropipettor

Parts of the Pipettor

• 1) yellow pushbutton• 2) calibration knob• 3) digital volume display• 4) barrel• 5) disposable pipette tip• 6) tip ejector

3

5

6

1

4

6

1

62

3

4

5

Use of the Labnet Micropipettor

Volume AdjustmentStep 1

• In the display window are three vertical numbers.

• The top number is tens µl (10.0).

• The middle number is ones µl (02.0).

• The bottom number tenths µl (00.9).

Use of the Labnet Micropipettor

Volume AdjustmentStep 2

• Set the pipettor to the desired volume by turning the calibration knob until the volume display reads the desired numbers.

• 7.5 µl would read from top to bottom in the display window: 0 - tens 7 - ones .5 - tenths

Use of the Labnet Micropipettor

Transferring LiquidsStep 1

• Adjust the pipettor to the desired volume.

• Hold the pipettor so the display is facing the user and the thumb can easily depress the yellow pushbutton.

Use of the Labnet Micropipettor

Transferring LiquidsStep 2

• Open the box of yellow pipette tips supplied by the Office of Biotechnology.

• Place the pipette cone (end) into one of the tips and press down firmly.

• NEVER USE THE PIPETTOR WITHOUT A TIP!

Use of the Labnet Micropipettor

Transferring LiquidsStep 3

• Lift the pipettor and attached tip away from the tip box.

• Depress the pushbutton to the first stop before placing the pipette tip in the liquid.

• Place the tip in the liquid.

Use of the Labnet Micropipettor

Transferring LiquidsStep 4

• Slowly release the pushbutton and draw the liquid into the tip.

• Remove the tip from the liquid and visually check for liquid in the tip.

Use of the Labnet Micropipettor

Transferring LiquidsStep 5

• Place the tip into the container which is to receive the liquid.

• Slowly depress the pushbutton as far as it can go.

• With the pushbutton fully depressed, withdraw the tip.

Use of the Labnet Micropipettor

Transferring LiquidsStep 6

• Press down on the tip ejector button to eject the tip.

• Dispose of the tip in an appropriate container.

Use of the Labnet Micropipettor