USING PROTEOMICS TOOLS

(DIGE)

TO STUDY OSTEOARTHRITIS

PHYSIOPATOLOGY

Benjamín Fernández-Gutiérrez

Servicio de Reumatología

DNA GENOMICS

RNA TRANSCRIPTOMICS

PROTEIN PROTEOMICS

LARGE-SCALE ANALYSIS

DIGE (Differential In-Gel Electrophoresis)

DIGE (Differential In-Gel Electrophoresis)

Aigner et al 2006

Aigner et al 2006

E U R O P E A N M U S C U L O S K E L E T A L R E V I E W2009

KEY POINTS

PROTEOMICS, PARTICULARLY DIGE, IS A USEFUL TOOL TO STUDY RHEUMATIC DISEASES AS OSTEOARTHRITIS

SPECIFIC OR ‘HYPOTHESIS GENERATING’ PROTEOMICS APPROACHESCAN BE USED TO ADDRESS SCIENTIFIC QUESTIONS

COMBINATION OF DIFFERENT TECHNIQUES (PROTEOMICS, GENOMICS, MOLECULAR, BIOLOGICAL AND OTHERS) WILL BE PROBABLY THE GOLD STANDARD TO ACHIEVE VALUABLE RESULTS

DIFFERENT POINT OF VIEWS AND DIFFERENT SPECIALISTS ARE CLEARLY NECESSARIES TO CLARIFY THE PHYSIOPATOLOGY OF COMPLEX DISEASESAS OSTEOARTHRITIS REALLY IS

CENTRO NACIONAL INVESTIGACIONES CARDIOVASCULARES.

Unidad de Proteómica

HOSPITAL CLÍNICO SAN CARLOS.

Servicio de Reumatología

Servicio de Traumatología

Emilio Camafeita

Enrique Calvo

Juan Antonio López

José Ramón Lamas

Maria Pilar Tornero-Esteban

Luis Rodriguez

Esther Villafuertes

Jose Antonio Hoyas

Fernando Marco