uv visible instrument types
TRANSCRIPT
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TYPES OF INSTRUMENTS
UV-VISIBLE SPECTROSCOPY
PRESENTED BY THUSHARA MOHAN, M PHARM, GRACE COLLEGE
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1.Single beam UV-Visible spectrophotometer
2.Double beam UV-Visible spectrophotometer
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SINGLE BEAMSPECTROPHOTOMETER
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SINGLE BEAM INSTRUMENT
5
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Source of light
Filter/Mono
chromatorsSample
cells
Detectors
Read out device
CONSTRUCTION
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WORKING
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DISADVANTAGES
1.Fluctuation
2.Rapid scanning is not possible
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DOUBLE BEAM SPECTROPHOTOMETER
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CONSTRUCTION
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Source of light
Filters/Mono
chromatorsSample cell
Reference cell D1
Read out deviceD2
Beam spliter
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