v. m. manikandamathavan , n. duraipandyb, m.s. kiran*,b, v ... · fig. s3.luminescence spectra of...

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A New Platinum(II) complex for Bioimaging Applications V. M. Manikandamathavan a , N. Duraipandy b , M.S. Kiran* ,b , V.G. Vaidyanathan* c,d and B. U. Nair * , a a Chemical Laboratory , b Biomaterials Laboratory, c Biophysics Laboratory and d Academy of Scientific and Innovative Research (AcSIR)CSIR-Central Leather Research Institute, Adyar, Chennai 600020, Tamil Nadu, India Electronic Supplementary Material (ESI) for RSC Advances. This journal is © The Royal Society of Chemistry 2015

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Page 1: V. M. Manikandamathavan , N. Duraipandyb, M.S. Kiran*,b, V ... · Fig. S3.Luminescence spectra of complex 2 (50 µM) in the absence and presence of CT DNA (0-800 µM) in Tris buffer

A New Platinum(II) complex for Bioimaging Applications V. M. Manikandamathavana, N. Duraipandyb, M.S. Kiran*,b, V.G. Vaidyanathan*c,d and B. U. Nair *, a aChemical Laboratory ,bBiomaterials Laboratory, cBiophysics Laboratory and dAcademy of Scientific and Innovative Research (AcSIR)CSIR-Central Leather Research Institute, Adyar, Chennai 600020, Tamil Nadu, India

Electronic Supplementary Material (ESI) for RSC Advances.This journal is © The Royal Society of Chemistry 2015

Page 2: V. M. Manikandamathavan , N. Duraipandyb, M.S. Kiran*,b, V ... · Fig. S3.Luminescence spectra of complex 2 (50 µM) in the absence and presence of CT DNA (0-800 µM) in Tris buffer

Figure S1. ESI-MS spectrum of complex 1

Page 3: V. M. Manikandamathavan , N. Duraipandyb, M.S. Kiran*,b, V ... · Fig. S3.Luminescence spectra of complex 2 (50 µM) in the absence and presence of CT DNA (0-800 µM) in Tris buffer

Figure S2. ESI-MS spectrum of complex 2

Page 4: V. M. Manikandamathavan , N. Duraipandyb, M.S. Kiran*,b, V ... · Fig. S3.Luminescence spectra of complex 2 (50 µM) in the absence and presence of CT DNA (0-800 µM) in Tris buffer

Fig. S3. Luminescence spectra of complex 2 (50 µM) in the absence and presence of CT DNA (0-800 µM) in Tris buffer (10 mM, pH 7.2). λex: 400 nm

Page 5: V. M. Manikandamathavan , N. Duraipandyb, M.S. Kiran*,b, V ... · Fig. S3.Luminescence spectra of complex 2 (50 µM) in the absence and presence of CT DNA (0-800 µM) in Tris buffer

Figure S4. Scatchard plot of complex 1 with DNA

Page 6: V. M. Manikandamathavan , N. Duraipandyb, M.S. Kiran*,b, V ... · Fig. S3.Luminescence spectra of complex 2 (50 µM) in the absence and presence of CT DNA (0-800 µM) in Tris buffer

Figure S5. Biocompatibility of cis-platin on HaCaT and A431 cell lines

Page 7: V. M. Manikandamathavan , N. Duraipandyb, M.S. Kiran*,b, V ... · Fig. S3.Luminescence spectra of complex 2 (50 µM) in the absence and presence of CT DNA (0-800 µM) in Tris buffer

Figure S6. Representative fluorescence image showing the localization of complex 1 in the nucleus

Page 8: V. M. Manikandamathavan , N. Duraipandyb, M.S. Kiran*,b, V ... · Fig. S3.Luminescence spectra of complex 2 (50 µM) in the absence and presence of CT DNA (0-800 µM) in Tris buffer

A B Membrane blebbing

Nuclear fragmentation

Figure S7. Detection of apoptotic features in HaCaT cells using complex 1 (a) phase contrast and (b) fluorescence image.