v national conference bifi, zaragoza, febrero 2011
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V National Conference BIFI, Zaragoza, Febrero 2011. OVERVIEW OF EXPERIMENTAL FACILITIES AT BIFI-BIO. OLGA ABIAN [email protected]. V National Conference BIFI, Zaragoza, Febrero 2011. GENERAL AREAS OR ACTIVITIES PERFORM AT BIFI FACILITIES. - PowerPoint PPT PresentationTRANSCRIPT
V National Conference BIFI, Zaragoza, Febrero 2011
OLGA [email protected]
OVERVIEW OF EXPERIMENTAL FACILITIES AT BIFI-BIO
V National Conference BIFI, Zaragoza, Febrero 2011
Molecular Biology: Genetic engineering and recombinant proteins (cloning, mutagenesis, recombinant proteins expression with biotechnology or biomedical interest).
Biochemistry: Proteins and nucleic acids purification using Fast Performance Liquid Chromatography, ionic exchange, affinity or molecular exclusion columns. Small protein or chemical compounds purification.
Biophysics of Biological Molecules: Characterization of protein stability, protein-protein and protein ligand interaction, and protein function.
Cell Biology: Prokaryotic and eukaryotic cell culture for the expression of recombinant proteins, determination of the efficacy and toxicity of bioactive compounds and apoptosis studies. Stem cell cultures for cellular differentiation and dead cell studies.
High Throughput Screening (HTS): Screening methods for experimental identification of bioactive compounds against pharmacologic targets.
Crystallography: Obtaining crystallographic models of proteins: preparation of crystals, analysis by X ray diffraction, data processing and molecular structure resolution.
GENERAL AREAS OR ACTIVITIES PERFORM AT BIFI FACILITIES
V National Conference BIFI, Zaragoza, Febrero 2011
LABORATORY USERS AT BIFI
V National Conference BIFI, Zaragoza, Febrero 2011
RESEARCH GROUPS USERS OF BIFI FACILITIES
V National Conference BIFI, Zaragoza, Febrero 2011
NATIONAL COLLABORATORS
CIPF VALENCIAMª Eugenia Armengod
UAB BARCELONASalvador VenturaMireia García-Viloca
Univ. País VascoArturo MugaMª Ángeles UrbanejaOscar Millet
CSIC Rocasolano MADRIDJulia Sanz-AparicioJuan Hermoso Mª Ángeles Jiménez
UMH Elche ALICANTEJose Luis Neira
C.S.I.C. C.I.B. MADRIDÁngel MartínezAitor Hernández-Ortega
Barcelona Supercomputing Centre BARCELONA
Víctor Guallar
Instituto de Bioquímica Vegetal y Fotosíntesis, SEVILLA
Miguel A. de la RosaAntonio Díaz Quintana
UNIZAR ZARAGOZAJesús MartínezMiguel Pocoví
INIBICA CORUÑA
Jose Francisco Pérez Llerena
Universidad Pablo Olavide SEVILLA
Laura Ledesma
Hospital ClínicoZARAGOZA
Ángel LanasTrinidad Serrano
UAM MADRIDMiguel Fernández Moreno Rafael Garesse AlarcónJuan J. Arredondo Lamas
I3A ZARAGOZALuis José Fernández
INA ZARAGOZAJesús Martínez De La FuenteValeria Grazú
CBM CSIC MADRIDCelia Perales
INSTITUTO DE CATÁLISIS CSIC MADRID
Jose Miguel PalomoJose Manuel Guisan
V National Conference BIFI, Zaragoza, Febrero 2011
NATIONAL UNIVERSITY OF SINGAPORE (Singapur)Jayaramam Sivaraman
U. COIMBRA (PORTUGAL)Maria Joao Moreno
Rui M. Brito
U. COPENHAGEN (DINAMARCA)
Marina Kasimova
JHU BALTIMORE (EEUU)Ernesto Freire
UNAM MEXICO(MEXICO)
Enrique García-HernándezAlfredo Lagunas
U. COLONIA (ALEMANIA)Agapios Sachinidis
Emory University, Atlanta (EEUU)
Dale Edmondson
U. VIRGINIA(EEUU)
Paul S. Hoffman
Stem Cell Center, New York (EEUU)
Michael Xavier Doss Jesudoss
INTERNATIONAL COLLABORATORS
V National Conference BIFI, Zaragoza, Febrero 2011
We moved to the new I+D building in February- Marzo 2010.
Laboratories were distributed according to techniques and equipment.
This allows the researches an easier use of the facilities and an efficient space distribution.
New incorporation of researchers and equipments is easier.
Biophysics, Biochemistry and Molecular and Cellular Biology sections at BIFI are distributed in laboratories dedicated to specific subjects.
They are distributed in block number 2 and floor 0 and block number 1 and floor 2 of the I+D building.
I+D BUILDING IN CAMPUS ACTUR OF ZARAGOZA UNIVERSITY ORGANIZATION
V National Conference BIFI, Zaragoza, Febrero 2011
Block 2 Floor 0
V National Conference BIFI, Zaragoza, Febrero 2011
Block 1 Floor 2
V National Conference BIFI, Zaragoza, Febrero 2011
12 individual working places composed by a desk and laboratory bench. Predoctoral students are working in this laboratory.
Laboratorio general I
V National Conference BIFI, Zaragoza, Febrero 2011Laboratorio de espectroscopia
Fluorescence spectrophotometer:
Cary Eclipse (Varian)Multi-sampler Peltier
module
UV/visible Spectrophotometer Cary 100
BIO (Varian)Multi-sample Peltier module
Espectrofotometer NanoVue (GE Healthcare)
Micro-volume samples
Light scattering Dynapro Nanostar (Wyatt)
Hydrodinamic radius determination and molecular
protein mass.
Spectropolarimeter and fluorescence spectrophotometer: Chirascan from Applied Photophysics. Structural characterization of proteins in steady state and kinetic state (stopped-Flod module)
V National Conference BIFI, Zaragoza, Febrero 2011Laboratorio de calorimetría y SPR
Differential Scanning Calorimeter VP-DSC
(MicroCal, GE Healthcare)Study of energetic structural stability
of proteins
2 units of Isothermal Titration Calorimeters VP-ITC
(MicroCal, GE Healthcare)Study of energetic biomolecule
interaction
Surface Plasmon Resonance Equipment Biacore T200 (GE
Healthcare)Study of biomolecules interaction in
kinetic and steady-state
Isothermal Titration Calorimeter AUTO-ITC200(MicroCal, GE Healthcare)
Study of energetic biomolecule interaction. Multi-sample and
automatic
V National Conference BIFI, Zaragoza, Febrero 2011Cámara fría 4ºC y cámara -20ºC
In the temperature controlled room (2.0.04) the storage of the samples at 4ºC or -20ºC is possible. The size of the room allows also to perform experiments such as protein purification or separation and analysis, using electrophoresis and chromatographic techniques.
V National Conference BIFI, Zaragoza, Febrero 2011Laboratorio de Rayos X
Automatic systems of liquid dispensation of liquids for macromolecules crystallization and management system and well visualization.A/ PERKIN ELMER JANUS MINI.B/ INNOVADYNE NANODROP II. C/ FORMULATRIX ROCK IMAGER 182.D/ LEICA Stereoscopies.
X-Ray DiffractometerAnalysis of crystal proteins
V National Conference BIFI, Zaragoza, Febrero 2011Laboratorio de cromatografía y microscopía
The Leica DMI6000 B inverted microscope for biomedical research offers Differential Interference Contrast (DIC) for relief imaging of specimens with varying indices of refraction. The intuitive, automated Leica DMI6000 B is ideal for fluorescence, live cell, time-lapse imaging; high-speed multi-fluorescence optical sectioning; and micro-manipulation.
2 Fast protein liquid chromatography equipments (FPLC)
Technique used for the separation or purification of proteins and other polymers from complex mixtures.
High-performance liquid chromatography equipment (HPLC)
Technique used for identifying, quantifying and purifying the individual components of the mixture.
V National Conference BIFI, Zaragoza, Febrero 2011Laboratorio general II
6 individual working laboratory bench. Senior researchers and technicians are working in this laboratory.
V National Conference BIFI, Zaragoza, Febrero 2011Laboratorio Biología Molecular
Laboratory with contains the general equipment for performing molecular biology experiments:
2 PCR thermal cyclers. DNA and protein electrophoresis system. Protein immunoblot Inmunoblot system. 2D Electrophoresis system Electroporator used for the transformation of bacteria, yeast. Purification water system Elix 3 (Millipore) and Ultrapure purification system MilliQ (Millipore). Ice maker.Manipulation yeast cabin.Safety chemistry cabin.
V National Conference BIFI, Zaragoza, Febrero 2011Laboratorio Cristalografía
This is a thermostatic chamber at 18ºC temperature with an anaerobic system and a cold room at 4ºC.
V National Conference BIFI, Zaragoza, Febrero 2011Sala de Congeladores y revelado
V National Conference BIFI, Zaragoza, Febrero 2011Laboratorio de Cultivos Celulares I
V National Conference BIFI, Zaragoza, Febrero 2011Laboratorio de Centrifugación
V National Conference BIFI, Zaragoza, Febrero 2011Laboratorio de Cribado
This laboratory contains: Synergy HT Multi-Mode Microplate Reader A filter-based high temperature fluorescence microplate reader FluoDia T70 Multi-plate well centrifuge
V National Conference BIFI, Zaragoza, Febrero 2011Laboratorio de Cultivo Celular
V National Conference BIFI, Zaragoza, Febrero 2011
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