variable wavelength detectors...thermoscientific vanquish variable wavelength detectors vc-d40,...

thermoscientific

Vanquish

Variable WavelengthDetectorsVC-D40, VF-D40

Operating Manual4820.7701-EN Revision 2.0 • December 2019

Copyright © 2019 Thermo Fisher Scientific Inc. All rights reserved.

Original Operating Manual

The hardware descriptions in this manual revision refer to devices VC-D40-A, VF-D40-A.

Trademarks

Acrobat, Adobe, and Adobe Reader are trademarks of Adobe Systems Incorporated.Microsoft and Windows are trademarks of Microsoft Corporation.MP35N is a trademark of SPS Technologies.Torx is a trademark of Acument Intellectual Properties, LLC.All other trademarks are property of Thermo Fisher Scientific and its subsidiaries.

Disclaimer

Thermo Fisher Scientific Inc. provides this document to its customers with a product purchase touse in the product operation. The document is copyright protected; any reproduction of thewhole or any part of this document is strictly prohibited, except with the written authorizationof Thermo Fisher Scientific Inc.

This manual is provided "as is." The contents of this manual are subject to being changed,without notice, in future revisions.

Thermo Fisher Scientific Inc. makes no representations that this document is complete,accurate, or error-free. Thermo Fisher Scientific Inc. assumes no responsibility and will not beliable for any errors, omissions, damage, or loss that might result from any use of this document,even if the information in the document is followed properly.

This document is not part of any sales contract between Thermo Fisher Scientific Inc. and apurchaser. This document shall in no way govern or modify any Terms and Conditions of Sale.The Terms and Conditions of Sale shall govern all conflicting information between the twodocuments.

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Variable Wavelength Detectors (VC-D40, VF-D40) Operating Manual

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Contents


Contents1 Using this Manual ...................................................................... 11

1.1 About this Manual ................................................................................................ 12

1.2 Conventions.......................................................................................................... 131.2.1 Safety Messages....................................................................................... 131.2.2 Special Notices and Informational Notes ................................................. 131.2.3 Typographical Conventions ...................................................................... 14

1.3 Reference Documentation ................................................................................... 15

2 Safety......................................................................................... 172.1 Safety Symbols and Signal Words......................................................................... 18

2.1.1 Safety Symbols and Signal Words in This Manual .................................... 182.1.2 Observing this Manual ............................................................................. 182.1.3 Safety Symbols on the Device .................................................................. 192.1.4 Rating Plate .............................................................................................. 19

2.2 Intended Use ........................................................................................................ 20

2.3 Safety Precautions................................................................................................ 212.3.1 General Safety Information...................................................................... 212.3.2 Qualification of the Personnel ................................................................. 212.3.3 Personal Protective Equipment................................................................ 222.3.4 Electrical Safety Precautions .................................................................... 232.3.5 General Residual Hazards......................................................................... 232.3.6 In Case of Emergency ............................................................................... 26

2.4 Solvent and Additive Information ........................................................................ 272.4.1 General Compatibility .............................................................................. 272.4.2 Allowed pH Ranges................................................................................... 282.4.3 Allowed Concentrations ........................................................................... 282.4.4 Further Information ................................................................................. 29

2.5 Compliance Information....................................................................................... 30

3 Device Overview ........................................................................ 313.1 Detector Features................................................................................................. 32

3.2 Operating Principle............................................................................................... 33

3.3 Interior Components ............................................................................................ 35

3.4 Flow Cell ............................................................................................................... 36

3.5 Lamps ................................................................................................................... 38

Contents


3.6 Leak Detection...................................................................................................... 39

3.7 Operation ............................................................................................................. 40

4 Unpacking .................................................................................. 414.1 Unpacking............................................................................................................. 42

4.2 Scope of Delivery.................................................................................................. 44

5 Installation................................................................................. 455.1 Safety Guidelines for Installation ......................................................................... 46

5.2 Installing the Device ............................................................................................. 47

5.3 Site Requirements ................................................................................................ 495.3.1 Power Considerations .............................................................................. 495.3.2 Power Cord............................................................................................... 495.3.3 Condensation ........................................................................................... 50

5.4 Accessing the Interior Components ..................................................................... 51

5.5 Setting Up the Hardware...................................................................................... 525.5.1 System Arrangement................................................................................ 525.5.2 Connecting the Device ............................................................................. 535.5.3 Connecting the Power Cord ..................................................................... 55

5.6 Installing a VIS Lamp (Tungsten Lamp) (Optional)................................................ 56

5.7 Installing a Flow Cell ............................................................................................. 595.7.1 Removing the Diagnostic Cell................................................................... 595.7.2 Installing a Flow Cell without Connection Unit ........................................ 605.7.3 Installing a Flow Cell with Connection Unit.............................................. 60

5.8 Setting Up the Flow Connections ......................................................................... 625.8.1 General Information and Guidelines........................................................ 625.8.2 Guiding Capillaries and Tubing Through the System................................ 635.8.3 Installing the Partition Panel Plugs........................................................... 655.8.4 Connecting Fittings, Capillaries, and Tubing ............................................ 665.8.5 Flow Connections to the Flow Cell ........................................................... 685.8.6 Guiding Liquid Leaks to Waste ................................................................. 72

5.9 Turning On the Device .......................................................................................... 73

5.10 Setting Up the Device in the Software ................................................................. 74

6 Operation................................................................................... 756.1 Introduction to this Chapter................................................................................. 76

6.2 Safety Guidelines for Operation ........................................................................... 77

Contents


6.3 Control Elements .................................................................................................. 786.3.1 Keypad...................................................................................................... 786.3.2 Status Indicators....................................................................................... 79

6.4 Power On/Off Control .......................................................................................... 81

6.5 Preparing the Device for Operation ..................................................................... 826.5.1 Guidelines for Use of Flow Cells ............................................................... 846.5.2 Turning On the Lamps .............................................................................. 85

6.6 Operational Modes of the Device......................................................................... 866.6.1 Single-Channel Mode ............................................................................... 866.6.2 Multi-Channel Mode ................................................................................ 86

6.7 Important Operating Parameters ......................................................................... 88

6.8 Optimizing the Performance of the Device .......................................................... 906.8.1 General Guidelines ................................................................................... 906.8.2 Overview of Optimization Parameters..................................................... 916.8.3 Wavelength .............................................................................................. 916.8.4 Data Collection Rate................................................................................. 926.8.5 Response Time and Peak Width............................................................... 926.8.6 Baseline Behavior ..................................................................................... 93

6.9 Shutting Down the Device .................................................................................... 946.9.1 Short-Term Shutdown (Interruption of Operation) ................................. 946.9.2 Long-Term Shutdown............................................................................... 95

7 Maintenance and Service ........................................................... 997.1 Introduction to Maintenance and Service......................................................... 100

7.2 Safety Guidelines for Maintenance and Service................................................ 101

7.3 General Rules for Maintenance and Service ..................................................... 103

7.4 Routine and Preventive Maintenance............................................................... 1047.4.1 Maintenance Plan .................................................................................. 1047.4.2 Cleaning or Decontaminating the Device............................................... 1047.4.3 Predictive Performance.......................................................................... 106

7.5 Lamps ................................................................................................................ 1077.5.1 Replacing the UV Lamp (Deuterium Lamp) ............................................ 1077.5.2 Replacing the VIS Lamp (Tungsten Lamp) .............................................. 110

7.6 Flow Cell ............................................................................................................ 1147.6.1 Guidelines for Handling Flow Cells ......................................................... 1157.6.2 Cleaning the Flow Cell ............................................................................ 1157.6.3 Removing the Flow Cell .......................................................................... 1167.6.4 Installing a Diagnostic Cell...................................................................... 118

Contents


7.7 Performing a Wavelength Validation and Calibration....................................... 119

7.8 Replacing the Waste Line .................................................................................. 121

7.9 Replacing the Main Power Fuses....................................................................... 122

7.10 Updating the Device Firmware.......................................................................... 124

7.11 Replacing the Doors........................................................................................... 126

7.12 Transporting or Shipping the Device ................................................................. 1287.12.1 Preparing the Device for Transport........................................................ 1287.12.2 Transporting the Device to a New Location ........................................... 1297.12.3 Shipping the Device................................................................................ 130

7.13 Replacing the Slide-In Module........................................................................... 1317.13.1 Removing the Slide-In Module ............................................................... 1317.13.2 Returning the Slide-In Module ............................................................... 1327.13.3 Installing the Slide-In Module ................................................................ 1337.13.4 Setting Up the Slide-In Module .............................................................. 135

8 Troubleshooting....................................................................... 1378.1 General Information about Troubleshooting .................................................... 138

8.2 Messages ........................................................................................................... 140

8.3 Checking the Flow Cell....................................................................................... 145

8.4 Resolving Liquid Leaks ....................................................................................... 146

9 Specifications ........................................................................... 1499.1 Performance Specifications............................................................................... 150

9.1.1 Detector ................................................................................................. 1509.1.2 Flow Cells................................................................................................ 152

9.2 Physical Specifications ....................................................................................... 153

10 Accessories, Consumables and Replacement Parts ................... 15510.1 General Information.......................................................................................... 156

10.2 Ship Kit............................................................................................................... 157

10.3 Optional Accessories ......................................................................................... 158

10.4 Consumables and Replacement Parts ............................................................... 159

11 Appendix.................................................................................. 16111.1 Compliance Information.................................................................................... 162

11.1.1 Declarations of Conformity .................................................................... 16211.1.2 WEEE Compliance .................................................................................. 163

Contents


11.1.3 FCC Compliance...................................................................................... 16311.1.4 NIST Compliance .................................................................................... 16311.1.5 Manual Release History.......................................................................... 163

11.2 UV Cutoff Wavelengths of Solvents................................................................... 165

11.3 Digital I/O .......................................................................................................... 166

Index........................................................................................ 169

Contents


1 • Using this Manual


1 Using this ManualThis chapter provides information about this manual, the conventionsused throughout the manual, and the reference documentation that isavailable in addition to this manual.



1.1 About this ManualThis manual describes the functional features and operating principle ofyour Vanquish™ device and provides instructions for installation, set up,start up, shut down, operation, maintenance and troubleshooting.

The layout of this manual is designed to provide quick reference to thesections of interest to the user. To obtain a full understanding of yourdevice, read this manual thoroughly.

This manual also contains safety messages, precautionary statements,and special notices that can prevent personal injury, damage to thedevice, or loss of data when followed properly.

Note the following:

• The device configuration may vary; therefore, not all descriptionsnecessarily apply to your particular device.

• If some detail applies to only one model or variant, the model orvariant is identified by name.

• Illustrations in this manual are provided for basic understanding.They can vary from the actual model of the device or component.However, this does not influence the descriptions. No claims can bederived from the illustrations in this manual.

The descriptions in this manual assume that the device is installed in theVanquish system stack. If this is not the case, additional hardware isrequired and must be ordered separately. The information in thismanual applies correspondingly.



1.2 ConventionsThis section describes the conventions that are used throughout thismanual.

1.2.1 Safety Messages

The safety messages and precautionary statements in this manualappear as follows:

• Safety messages or precautionary statements that apply to theentire manual and all procedures in this manual are grouped in theSafety chapter.

• Safety messages or precautionary statements that apply to an entiresection or to multiple procedures in a section appear at thebeginning of the section to which they apply.

• Safety messages that apply to only a particular section or procedureappear in the section or procedure to which they apply. They appeardifferent from the main flow of text.

Safety messages are often preceded by an alert symbol and/or alertword. The alert word appears in uppercase letters and in bold type.

Make sure that you understand and follow all safety messagespresented in this manual.

1.2.2 Special Notices and Informational Notes

Special notices and informational notes in this manual appear differentfrom the main flow of text. They appear in boxes and a note labelidentifies them. The label text appears in uppercase letters and in boldtype.

NOTICE

Highlights information necessary to prevent damage to the device orinvalid test results.

TIP Highlights information of general interest or helpful information thatcan make a task easier or optimize the performance of the device.



1.2.3 Typographical Conventions

These typographical conventions apply to the descriptions in thismanual:

Data Input and Output

• The following appears in bold type:

¨ Input that you enter by the keyboard or that you select with themouse

¨ Buttons that you click on the screen

¨ Commands that you enter by the keyboard

¨ Names of, for example, dialog boxes, properties, and parameters

• For brevity, long expressions and paths appear in the condensedform, for example: Click Start > All Programs > ThermoChromeleon 7 > Services Manager > Start Instrument Controller.

References and Messages

• References to additional documentation appear italicized.

• Messages that appear on the screen are identified by quotationmarks.

Viewpoint

If not otherwise stated, the expressions left and right in this manualalways refer to the viewpoint of a person that is facing the device fromthe front.

Particularly Important Words

Particularly important words in the main flow of text appear italicized.

Electronic Manual Version (PDF)

The electronic version (PDF) of the manual contains numerous links thatyou can click to go to other locations within the manual. These include:

• Table of contents entries

• Index entries

• Cross-references (in blue text), for example, to sections and figures



1.3 Reference DocumentationIn addition to this operating manual, other documentation is availablefor reference.

Hardware Documentation

Additional hardware documentation includes the following:

• Operating manuals for the other modules of the Vanquish systemA printed version of the manual is shipped with the device.

• Vanquish System Operating ManualA printed version of the manual is shipped with the Vanquish systembase and solvent rack.

• Instrument Installation Qualification Operating Instructions

TIP Electronic versions of these manuals are available as PDF (PortableDocument Format) files. To open and read the PDF files, Adobe™Reader™ or Adobe™ Acrobat™ is required.

Software Documentation

Additional software documentation includes the following:

• Chromeleon™ Help and documentsThe Chromeleon Help provides extensive information andcomprehensive reference material for all aspects of the software.

In addition, the following documentation is available (availabilitydepends on the software version):

• Installation GuideFor basic information about device installation and configuration,refer to the Installation Guide.

• Instrument Configuration Manager HelpFor specific information about a certain device, refer to theInstrument Configuration Manager Help. In Chromeleon 7, devicesare called modules.

• Quick Start GuideFor information about the main elements of the user interface andstep-by-step guidance through the most important workflows, referto the Quick Start Guide.

• Reference CardFor a concise overview of the most important workflows, refer tothe Reference Card.



TIP The Chromeleon Help and documents are included in the softwareshipment.

Third-Party Documentation

Refer also to the user documentation provided by the manufacturers ofthird-party components and materials, for example, Safety Data Sheets(SDSs).

2 • Safety


2 SafetyThis chapter provides general and specific safety information andinforms about the intended use of the device.

2 • Safety


2.1 Safety Symbols and Signal Words

2.1.1 Safety Symbols and Signal Words in This Manual

This manual contains safety messages to prevent injury of the personsusing the device.

The safety symbols and signal words in this manual include thefollowing:

Always be aware of the safety information. Do not proceed until youhave fully understood the information and consider the consequences ofwhat you are doing.

CAUTION

Indicates a hazardous situation that, if not avoided, could result in minoror moderate injury.

WARNING

Indicates a hazardous situation that, if not avoided, could result inserious injury.

2.1.2 Observing this Manual

Observe the following:

• Before installing or operating the device, read this manual carefullyto be familiar with the device and this manual. The manual containsimportant information with regard to user safety as well as use andcare of the device.

• Always keep the manual near the device for quick reference.

• Save this manual and pass it on to any subsequent user.

Read, understand, and comply with all safety messages andprecautionary statements presented in this manual.

2 • Safety


2.1.3 Safety Symbols on the Device

The table lists the safety symbols that appear on the device or on labelsaffixed to the device. Follow the safety notices in this manual to preventthe risk of operator injury or damage to the device.

Symbol Description

Indicates a potential hazard. Refer to this manual to avoid the risk ofpersonal injury and/or to prevent damage to the device.

—Ο

Power supply is onPower supply is off

Indicates alternating current.

Indicates that the surface becomes hot during operation. Do nottouch these surfaces while they are heated up.

Indicates that the UV radiation produced by the deuterium lamp inthe device may be harmful to eyes and skin. Do not look directly intothe light produced by the deuterium lamp. Never operate the lampoutside the device.

2.1.4 Rating Plate

The rating plate is present on the device near the electrical connections.The rating plate indicates the serial number, part number, modulename, revision number (if any), line and fuse rating, and themanufacturer's address.

TIP An additional type label on the leak tray of the device indicates themodule name, serial number, part number, and revision number (if any).To facilitate device identification, have the information from this labelavailable when communicating with Thermo Fisher Scientific.

2 • Safety


2.2 Intended UseThe device is intended to be part of the Vanquish system.

The intended use of the Vanquish system is to analyze mixtures ofcompounds in sample solutions.

The device is for use by qualified personnel and in laboratoryenvironment only.

The device and Vanquish system are intended to be used as GeneralLaboratory Equipment (GLE).

They are not intended for use in diagnostic procedures.

Laboratory Practice

Thermo Fisher Scientific recommends that the laboratory in which theVanquish system is used follow best practices for LC analyses. Thisincludes among others:

• Using appropriate standards

• Regularly running calibration

• Establishing shelf life limits and following them for all consumablesused with the system

• Running the system according to the laboratory's verified andvalidated 'lab developed test' protocol

2 • Safety


2.3 Safety Precautions

2.3.1 General Safety Information

All users must observe the general safety information presented in thissection and all specific safety messages and precautionary statementselsewhere in this manual during all phases of installation, operation,troubleshooting, maintenance, shutdown, and transport of the device.

If the device is used in a manner not specified by Thermo FisherScientific, the protection provided by the device could be impaired.Observe the following:

• Operate the device only within its technical specifications.

• Use only the replacement parts and additional components, options,and peripherals specifically authorized and qualified for the deviceby Thermo Fisher Scientific.

• Perform only the procedures that are described in this operatingmanual and in supporting documents for the device. Follow allinstructions step by step and use the tools recommended for theprocedure.

• Open the enclosure of the device and other components only ifspecifically instructed to do so in this manual.

• Thermo Fisher Scientific cannot be held liable for any damage,material or otherwise, resulting from inappropriate or improper useof the device. If there is any question regarding appropriate usage,contact Thermo Fisher Scientific before proceeding.

Safety Standard

This device is a Safety Class I instrument (provided with terminal forprotective grounding). The device has been manufactured and testedaccording to international safety standards.

2.3.2 Qualification of the Personnel

Observe the information below on the proper qualification of thepersonnel installing and/or operating the device.

2 • Safety


Installation

Only skilled personnel are permitted to install the device and toestablish the electrical connections according to the appropriateregulations.

• Thermo Fisher Scientific recommends always having servicepersonnel certified by Thermo Fisher Scientific perform theinstallation (for brevity, referred to as Thermo Fisher Scientificservice engineer).

• If a person other than a Thermo Fisher Scientific service engineerinstalls and sets up the module, the installer is responsible forensuring the safety of the module and system.

General Operation

The device is designed to be operated only by trained and qualifiedpersonnel in a laboratory environment.

All users must know the hazards presented by the device and thesubstances they are using. All users should observe the related SafetyData Sheets (SDSs).

2.3.3 Personal Protective Equipment

Wear personal protective equipment and follow good laboratorypractice to protect you from hazardous substances. The appropriateequipment depends on the hazard. For advice on the hazards and theequipment required for the substances you are using, refer to thematerial handling and safety data sheet provided by the vendor.

An eyewash facility and a sink should be available nearby. If anysubstance contacts your skin or eyes, wash the affected area and seekmedical attention.

Protective Clothing

To protect you from chemical splashes, harmful liquids, or othercontamination, put on appropriate protective clothing, such as a labcoat.

Protective Eyewear

To prevent liquids from striking your eyes, put on appropriate protectiveeyewear, such as safety glasses with side shields. If there is a risk ofsplashing liquids, put on goggles.

2 • Safety


Gloves

To protect you from harmful liquids and avoid personal injury duringmaintenance or service, put on appropriate protective gloves.

2.3.4 Electrical Safety Precautions

WARNING—Electric Shock or Damage to the Device

High voltages are present inside the device that could cause an electricshock or damage to the device.

• Do not make any changes to the electrical or grounding connections.

• If you suspect any kind of electrical damage, disconnect the powercord and contact Thermo Fisher Scientific Technical Support forassistance.

• Do not open the housing or remove protective panels unlessspecifically instructed to do so in this manual.

• Do not place liquid reservoirs directly upon the device. Liquid mightleak into the device and get into contact with electronic componentscausing a short circuit. Instead, place liquid reservoirs in the solventrack that is available for the Vanquish system.

2.3.5 General Residual Hazards

Pay attention to the following general residual hazards when workingwith the device:

2 • Safety


WARNING—Hazardous Substances

Solvents, mobile phases, samples, and reagents might contain toxic,carcinogenic, mutagenic, infectious, or otherwise harmful substances.The handling of these substances can pose health and safety risks.

• Be sure that you know the properties of all substances that you areusing. Avoid exposure to harmful substances. If you have any doubtabout a substance, handle the substance as if it is potentiallyharmful.

• Wear personal protective equipment as required by the hazard andfollow good laboratory practice.

• Reduce the volume of substances to the minimum volume requiredfor sample analysis.

• Do not operate the device in a potentially flammable environment.

• Avoid accumulation of harmful substances. Make sure that theinstallation site is well ventilated.

• Dispose of hazardous waste in an environmentally safe manner thatis consistent with local regulations. Follow a regulated, approvedwaste disposal program.

WARNING—Biohazard

Biohazardous material, for example microorganisms, cell cultures,tissues, body fluids, and other biological agents can transmit infectiousdiseases. To avoid infections with these agents:

• Assume that all biological substances are at least potentiallyinfectious.

• Wear personal protective equipment as required by the hazard andfollow good laboratory practice.

• Dispose of biohazardous waste in an environmentally safe mannerthat is consistent with local regulations. Follow a regulated,approved waste disposal program.

WARNING—Self-Ignition of Solvents

Solvents with a self-ignition temperature below 150 °C might ignitewhen in contact with a hot surface (for example, due to leakage in thechromatography system).

Avoid the use of these solvents.

2 • Safety


WARNING—Hazardous Vapors

Mobile phases and samples might contain volatile or flammablesolvents. The handling of these substances can pose health and safetyrisks.

• Avoid accumulation of these substances. Make sure that theinstallation site is well ventilated.

• Avoid open flames and sparks.

• Do not operate the device in the presence of flammable gases orfumes.

CAUTION—Escape of Hazardous Substances from PEEK Capillaries

In the Vanquish system, capillaries made of PEEK may be used. Swellingor attack by acids can cause PEEK capillaries to start leaking or to burst.Certain chemicals, for example, trichlormethane (CHCl3), dimethylsulfoxide (DMSO), or tetrahydrofuran (THF) can cause PEEK to swell.Concentrated acids, such as sulfuric acid and nitric acid, or a mixture ofhexane, ethyl acetate, and methanol, can attack PEEK.

• Swelling or attack is not a problem with brief flushing procedures.

• For more information, refer to the technical literature on thechemical resistance of PEEK.

CAUTION—Allergic Reaction

Some capillaries in the Vanquish system are made of MP35N™, a nickel/cobalt-based alloy. Individuals with sensitivity to nickel/cobalt may showan allergic reaction from skin contact.

CAUTION—Sparking due to Electrostatic Discharge

Liquid flowing through capillaries can generate static electricity. Thiseffect is particularly present with insulating capillaries and non-conductive solvents (for example, pure acetonitrile). Discharge ofelectrostatic energy might lead to sparking, which could constitute a firehazard.

Prevent the generation of static electricity near the chromatographysystem.

2 • Safety


2.3.6 In Case of Emergency

WARNING—Safety Hazard

In case of emergency, disconnect the device from the power line.

2 • Safety


2.4 Solvent and Additive Information

2.4.1 General Compatibility

To protect optimal functionality of the Vanquish system, observe theserecommendations on the use of solvents and additives:

• The system must be used with reversed-phase (RP) compatiblesolvents and additives only.

• Use only solvents and additives that are compatible with all parts inthe flow path.

TIP In a Vanquish Core system, normal-phase (NP) compatible solventsand additives may be used if the VC-pumps and the VC-autosamplers aremodified with the components from the Normal-Phase (NP) kit. Refer tothe Operating Manuals for the pumps and autosamplers.

NOTICE

If the system flow path includes a Vanquish charged aerosol detector,observe the specific solvent and additive information for this detector.Refer to the Operating Manual for the Vanquish charged aerosoldetector.

2 • Safety


2.4.2 Allowed pH Ranges

Allowed pH ranges (standard system configuration):

System (StandardConfiguration)

AllowedpH ranges

Remarks

Vanquish Core 1-13 • pH values of 2 or less: The application time shouldbe as short as possible. Flush the systemthoroughly after these applications.

• pH values higher than 9.5 with optical detectors:Avoid using mobile phases with a pH value higherthan 9.5 together with optical detectors. This canimpair the functionality and optical performanceof the detector flow cell.

• pH values higher than 12: May affectelectrochemical detection. Before using highlyalkaline solvents for flushing the system,disconnect the detector from the system.

• Mobile phases containing ammonium hydroxide: Inrare cases, a shortened lifetime of reversed-phase(UHMW-PE) piston seals has been observed withhigh pH, ammonium hydroxide containing mobilephases and prolonged exposure.

VanquishHorizonVanquish Flex

2-12

2.4.3 Allowed Concentrations

Allowed concentrations (standard system configuration):

System (StandardConfiguration)

Chloride Buffer Remarks

Vanquish Core 0.1 mol/Lor less

1 mol/L orless

• High chloride concentration:The application time should beas short as possible. Flush thesystem thoroughly after theseapplications.

• Mobile phases containingammonium hydroxide: In rarecases, a shortened lifetime ofreversed-phase (UHMW-PE)piston seals has been observedwith high pH, ammoniumhydroxide containing mobilephases and prolongedexposure.

Vanquish HorizonVanquish Flex

1 mol/L orless

-

2 • Safety


2.4.4 Further Information

• For details about the materials that are used in the analytical flowpath of the device, see the Specifications chapter in this manual. Forinformation about the materials that are used in the flow path of theother modules in the Vanquish system, refer to the Specificationschapter in the Operating Manual for the modules.

• Follow any specific recommendations presented in other sections ofthis manual. Refer also to the operating manuals for all modules inthe Vanquish system. They may provide additional guidelines andinformation.

• Observe the general guidelines and recommendations on the use ofsolvents and additives in the chromatography system. Refer to Useof Solvents and Additives in the Vanquish System Operating Manual.

2 • Safety


2.5 Compliance InformationThermo Fisher Scientific performs complete testing and evaluation of itsproducts to ensure full compliance with applicable domestic andinternational regulations. When the device is delivered to you, it meetsall pertinent electromagnetic compatibility (EMC) and safety standardsas described in this manual.

Changes that you make to the device may void compliance with one ormore of these EMC and safety standards. Changes to the device includereplacing a part or adding components, options, or peripherals notspecifically authorized and qualified for the product by Thermo FisherScientific. To ensure continued compliance with EMC and safetystandards, replacement parts and additional components, options, andperipherals must be ordered from Thermo Fisher Scientific or one of itsauthorized representatives.

The device has been shipped from the manufacturing site in a safecondition.

See also

2 Compliance Information ( page 162)

3 • Device Overview


3 Device OverviewThis chapter introduces you to the device and the main components.



3.1 Detector FeaturesThe detector comprises the following main features:

• The detector is a dual-beam, variable wavelength photometer withone measurement and one internal reference beam.

• Two different light sources provide a wavelength detection rangefrom 190 nm to 750 nm (VC-D40) and from 190 nm to 900 nm (VF-D40):

¨ A deuterium lamp (referred to as UV lamp) for ultraviolet andvisible detection and

¨ A tungsten lamp (referred to as VIS lamp) for visible and near-infrared detection (optional for VC-D40)

• Internal validation of the wavelength accuracy with holmium oxidefilter

• Multi-Wavelength Operation for additional operation functions,measuring simultaneously at up to two (VC-D40) or four (VF-D40)different wavelengths

• Data collection rates of up to 125 Hz (VC-D40) and up to 250 Hz (VF-D40) under Chromeleon 7.2 software or later, in single-channelmode

• Spectra measurements

• Two optical filters for eliminating higher order lightTo ensure optimal measurement results over the entire wavelengthrange, the optical filters automatically move into the light path (alsoin multi-wavelength operation).

• Automatic wavelength calibration after power-up and when thelamp configuration was changed.

• A shutter (motorized filter paddle) to move into the light path beforethe flow cellThe shutter can move into the following positions:

¨ Open position for data acquisition

¨ Closed (dark) position for protection of the flow cell and for darksignal measurements

¨ Holmium oxide filter position for validation of wavelengthaccuracy

• Availability of flow cells for different fields of applications (fordetails, see Flow Cells ( page 152))



3.2 Operating PrincipleThe detector is a dual-beam spectrophotometer that is designed forultraviolet (UV), visible (VIS) and near-infrared (NIR) absorptionspectrophotometry in combination with HPLC or UHPLC separations.

Photometric detection bases upon the absorption of monochromaticlight. The optical system of the detector provides a deuterium lamp(UV), and may provide a tungsten lamp (VIS), which is optional for VC-D40 detectors. A concave mirror (no. 2) focuses the light from the VISlamp (no. 1) to the aperture of the UV lamp (no. 3). Another mirror (no.4) receives the combined light from both light sources and focuses it intothe entrance slit (no. 6). The shutter (motorized filter paddle, no. 5) canbe moved into the light path before the flow cell to protect the flow cell.

The light passes through the entrance slit to the mirror (no.7) and on tothe grating (no. 8), where the light beam is diffracted. The angularposition of the grating determines the selected wavelength that isfocused by the mirror (no.9) and directed to the beamsplitter (no. 10)and flow cell (no. 13).

The beamsplitter (no. 10) directs part of the light beam to the referencediode (no. 11). If the flow cell is removed, a safety shutter (no. 12) blocksthe light beam to protect the user from UV radiation. If a flow cell isinstalled, the remaining light travels through the flow cell (no. 13), and ispartly absorbed depending on the sample solution in the flow cell. Ittravels on to the measurement photodiode (no. 14), where theremaining light intensity is measured. The absorbance of the analyte iscalculated from the measured intensities based on the law of Lambert-Beer.

The response of the detector is proportional to the concentration of theanalyte. Simultaneously, the reference beam intensity is measured andused to compensate any intensity fluctuations of the light source.

The following picture shows the optics of the detector, and illustrateshow the detector operates:



Figure 1: Operating principle of the optics

No. Description

1 VIS lamp (tungsten lamp)

2 Mirror (VIS)

3 UV lamp (deuterium lamp)

4 Mirror (combined light)

5 Shutter

6 Entrance slit

7 Mirror

8 Grating

9 Mirror

10 Beamsplitter

11 Reference photodiode

12 Safety shutter

13 Flow cell

14 Measurement photodiode



3.3 Interior ComponentsThe user-accessible components of the device are located directlybehind the front doors:

1 2 3 4 5 6 7 8

9

10

11

Figure 2: Interior components (here with flow cell with connection unit installed)

No. Description

1 Cooling air intake

2 Keypad with status indicators

3 Screw holes for connection unit (left-side installation)

4 Flow cell with connection unit

5 Connection unit

6 Leak tray

7 Type label, indicating the module name, serial number, part number, andrevision number (if any)

8 Drain port

9 Leak sensor

10 Partition panelThe recesses in the partition panel are used to route capillaries with the helpof special plugs (see Installing the Partition Panel Plugs ( page 65)).

11 Lamp house coverDepending on the detector configuration, a UV lamp or a UV and a VIS lampare installed in the lamp house.



3.4 Flow CellThe detector design allows easy access to the flow cell on the interiorfront.

1

1

24

6

3

5

Figure 3: Flow cell (example)

No. Description

1 Flow cell screws

2 Flow cell handle

3 Drain outlet

4 Connector for outlet capillary

5 Connection unit screw

6 Connector for inlet capillary

Flow Cell Label

One or more flow cell labels are present on the flow cell, which containinformation such as flow cell type, part number, and serial number.

Flow Cell Identification Chip

An identification (ID) chip on the flow cell stores information, includingthe flow cell type and the serial number of the flow cell. The ID chip alsostores data during operation, such as the exposure time to the light.

When the flow cell is installed, the detector reads the data from the chipand transfers the flow cell data to the Chromeleon software.

Heat exchanger

Standard flow cells and semi-micro flow cells are equipped with a built-in heat exchanger.



The heat exchanger helps to adapt the temperature of the mobile phaseto the flow cell temperature before the mobile phase enters the opticalflow path within the flow cell. Note that the volume of the heatexchanger and inlet capillary influences the retention times and peakwidths.

Flow Cell Types

The detector is shipped with a diagnostic cell. Replace the diagnostic cellwith a flow cell that is appropriate for your application. Depending onthe application needs, different types of flow cells are available for thedetector.

For flow cell ordering information, see Optional Accessories( page 158).

For the flow cell specifications, including, for example, materials in theflow path and pressure limit, see Flow Cells ( page 152).

For details about the flow cells or about the availability of other flowcells, refer to the Thermo Fisher Scientific sales organization.

See also

2 Guidelines for Use of Flow Cells ( page 84)



3.5 LampsLamp Types

Two different light sources provide the light for a wavelength detectionrange from 190 nm to 750 nm (VC-D40), or from 190 nm to 900 nm (VF-D40). Note that the VIS lamp is optional for VC-D40.

Refer to the table below to find out which lamp is recommended if atleast one of your detection wavelengths is within the range indicated inthe left column.

At least one detection wavelength Recommended lamp(s)

≤ 345 nm UV

Between 346 and 670 nm UV and VIS (turn on both lamps)

> 670 nm VIS

Identification Chip

Each lamp is equipped with an identification (ID) chip. The ID chip storesinformation about the lamp, including the number of lamp ignitions, andthe operating time of the lamp, thus providing an overview of the lampstatus.

When a lamp is installed, the ID chip is automatically connected to thedetector electronics.



3.6 Leak DetectionLeaks are a potential safety issue.

The leak sensor inside the device monitors the device for liquid leaksfrom the flow connections. The liquid is collected in the leak tray andguided to the drain port. From the drain port, the liquid is discharged towaste through the drain system of the Vanquish system.

When the leak sensor detects leakage, the status indicators change tored and beeping starts to alert you. Follow the instructions in thismanual to find and eliminate the source for the leakage.



3.7 OperationThe device is designed to be operated from a computer configured withthe Chromeleon Chromatography Data System (CDS). The Chromeleonsoftware provides complete instrument control, data acquisition, anddata management.

For a basic description of instrument control and automated sampleanalysis with the Chromeleon software, refer to the Vanquish SystemOperating Manual. Details on control and operation of the device areavailable in the Chromeleon Help.

TIP The device can be operated also with other data systems, such asThermo ScientificTM XcaliburTM. In this case, installation of additionalsoftware is required in addition to the data system software. For details,contact the Thermo Fisher Scientific sales organization.

A keypad is available inside the device, allowing you to perform certainbasic functions directly from the device.

4 • Unpacking


4 UnpackingThis chapter provides information for unpacking the device and informsyou about the scope of delivery.

4 • Unpacking


4.1 UnpackingDamaged Packaging, Defective on Arrival

Inspect the shipping container for signs of external damage and, afterunpacking, inspect the device for any signs of mechanical damage thatmight have occurred during shipment.

If you suspect that the device may have been damaged during shipment,immediately notify the incoming carrier and Thermo Fisher Scientificabout the damage. Shipping insurance will compensate for the damageonly if reported immediately.

Unpacking the Device

CAUTION—Heavy Load, Bulky Device

The device is too heavy or bulky for one person alone to handle safely.To avoid personal injury or damage to the device, observe the followingguidelines:

• Physical handling of the device, including lifting or moving, requiresa team effort of two persons.

• A team effort is in particular required when lifting the device intothe system stack or when removing it.

• Use the carrying handles that were shipped with the device to moveor transport the device. Never move or lift the device by the frontdoors. This will damage the doors or the device.

Tools required

Screwdriver, Torx™ T20

Follow these steps

1. Place the shipping container on the floor and open it.

2. Remove the ship kit from the shipping container.

3. Remove the device from the shipping container: Grasp the device bythe carrying handles. Slowly and carefully, lift the device out of theshipping container.

4 • Unpacking


Figure 4: Carrying handles on the device

No. Component

1 Carrying handles

2 Attachment screw (one on each carrying handle)

4. Place the device on a stable surface.

5. If applicable:Remove any additional packing material. Leave any protective filmsattached to the surfaces of the device until it is properly positionedin the system stack.

6. Transport the device by the carrying handles to the installation site,if it is not already there, and place it in the system stack (see SystemArrangement ( page 52)).

7. On each carrying handle, loosen the attachment screw until thecarrying handle is moveable in the rail. Do not remove the screwsfrom the carrying handles completely.

8. Slide off the carrying handles from the rails towards the rear of thedevice.

Figure 5: Sliding off the carrying handle from the left rail

TIP Keep the shipping container, the carrying handles with theattachment screws, and all packing material. These items will be neededif the device is transported to a new location or shipped.

9. Some surfaces including the doors of the device are covered by aprotective film during shipment. Remove the protective film from allsurfaces as applicable.

4 • Unpacking


4.2 Scope of DeliveryThe following items are included in the delivery:

• Detector

• Ship Kit

• Operating manual

• Power cord

For information on contents of the ship kit or reordering parts, seeAccessories, Consumables and Replacement Parts ( page 155).

5 • Installation


5 InstallationThis chapter specifies the requirements for the installation site anddescribes how to set up, install, and configure the device in the Vanquishsystem and in the chromatography software.

5 • Installation


5.1 Safety Guidelines for InstallationPay attention to the following safety guidelines:

Observe all warning messages and precautionary statements presentedin Safety Precautions ( page 21).






CAUTION—Electric Shock or Damage to the Device

After the power to the device is turned off, the device is still energizedas long as the power cord is connected. Repair work on the device whilethe device is connected to power could lead to personal injury.

• Always unplug the power cord before starting repair work inside thedevice.

• If you were instructed to remove any housing covers or panels, donot connect the power cord to the device while the cover or panelsare removed.

5 • Installation


5.2 Installing the DeviceThe Vanquish system is installed and set up by a Thermo Fisher Scientificservice engineer, including all modules and options or parts shipped withthem. The service engineer checks that the installation is correct andthat the Vanquish system and modules operate as specified. Theengineer also demonstrates the basic operation and main features.

If personnel other than a Thermo Fisher Scientific service engineerinstalls the device, follow the steps below.

NOTICE

The device is part of the Vanquish system. Therefore, follow the orderfor installing the system modules as described in the Vanquish SystemOperating Manual.

1. Pay attention to the safety guidelines and observe all siterequirements. See Safety Guidelines for Installation ( page 46) andSite Requirements ( page 49).

2. Set up the device hardware. See Setting Up the Hardware( page 52).

3. Set up the flow connections. See Setting Up the Flow Connections( page 62).

4. Turn on the device. See Turning On the Device ( page 73).

TIP

Before turning on the power to a Vanquish system module for the firsttime, verify that the chromatography software is installed on the datasystem computer. When the power is turned on, the required USBdrivers are automatically found and the Windows™ operating systemcan detect the device.

5. Set up the device in the software. See Setting Up the Device in theSoftware ( page 74).

6. Recommended:Perform Instrument Installation Qualification.

In the Chromeleon software, a wizard is available to guide youthrough the qualification process. On the Chromeleon 7 Console:Click Tools > Instrument Qualification > Installation Qualification.

Follow the instructions in the Instruments Installation QualificationOperating Instructions. The manual provides information about therequired materials and detailed instructions.

5 • Installation


NOTICE

If the device is operated with another data system, refer to thedocumentation for the software that you are using and/or perform thequalification manually. The Instruments Installation QualificationOperating Instructions provide information about the parameters to beadapted and the required settings.

7. Recommended: Perform Operational Qualification.The qualification kit includes all materials required for thequalification and detailed instructions.

Moving the Device after Installation

If you have to move the device after it has been set up and installed inthe Vanquish system, prepare the device for transport and move it tothe new location. Follow the instructions in Transporting or Shipping theDevice ( page 128).

5 • Installation


5.3 Site RequirementsThe operating environment is important to ensure optimal performanceof the device. This section provides important requirements for theinstallation site. Note the following:

• Operate the device only under appropriate laboratory conditions.

• The device is intended to be part of the Vanquish system. Observethe site requirements for the Vanquish system as stated in theVanquish System Operating Manual.

• For specifications, see Specifications ( page 149) and theSpecifications sections in the Operating Manuals for the othermodules in the Vanquish system.

• For general residual hazards, see General Residual Hazards( page 23).

5.3.1 Power Considerations

The power supply of the device has wide-ranging capability, acceptingany line voltage in the range specified for the device.


Connecting the device to a line voltage higher or lower than specifiedcould result in personal injury or damage to the device.

Connect the device to the specified line voltage only.

5.3.2 Power Cord

The power cords are designed to match the wall socket requirements ofthe country in which they are used. The end of the power cords thatplugs into the power socket on the device is identical for all power cords.The end of the power cords that plugs into the wall socket is different.

5 • Installation


WARNING—Electric Shock or Damage to the Device

• Never use a power cord other than the power cords provided byThermo Fisher Scientific for the device.

• Only use a power cord that is designed for the country in which youuse the device.

• Do not use extension cords.

• Never plug the power cord to a power socket that is shared withother equipment (for example, multiple sockets).

• Operate the device only from a power outlet that has a protectiveground connection.

• In case of emergency, it must be possible to reach the power cordeasily at any time to disconnect the device from the power line.

WARNING—Electric Shock or Damage to a Product

Misuse of the power cords could cause personal injury or damage theinstrument. Use the power cords provided by Thermo Fisher Scientificonly for the purpose for which they are intended. Do not use them forany other purpose, for example, for connecting other instruments.

5.3.3 Condensation

NOTICE—Condensation in the device can damage the electronics andoptics.

• When using, shipping, or storing the device, avoid or minimizeconditions that can lead to a build-up of condensation in the device.For example, avoid significant or fast changes in environmentalconditions.

• If you suspect that condensation is present, allow the device towarm up to room temperature. This may take several hours. Waituntil the condensation is gone completely before connecting thedevice to the power line.

5 • Installation


5.4 Accessing the Interior ComponentsTo access the interior components in the device, open the front doors.To allow easy access from the front, the user-accessible components andflow connections in the device are located directly behind the doors.

Figure 6: Opening the front doors

5 • Installation


5.5 Setting Up the HardwareThis section describes how to set up the hardware and providesinformation about the device connectors and cables.

5.5.1 System Arrangement

The device is part of the Vanquish system. The system modules aretypically arranged in a system stack, with the arrangement depending onthe system configuration.

Figure 7: Vanquish system, standard configuration (example)

No. Description

1 Solvent Rack

2 Detector

3 Autosampler

4 Pump

5 System Base

6 Column Compartment

5 • Installation


For instructions on how to set up the system stack, refer to the VanquishSystem Operating Manual.

5.5.2 Connecting the Device

Device Connectors

The following connectors are provided on the device:

Figure 8: Electrical connectors on the right side of the detector

No. Description

1 Rating plate, indicating the serial number, part number, module name,revision number (if any), line and fuse rating, and the manufacturer'saddress

2 Main power switch (on/off control)

3 Fuse holder

4 Power-inlet connector

5 • Installation


No. Description

5 System Interlink portAllows power on/off control for the detector from the Vanquish systembase and device communication and synchronization between the detectorand other modules in the Vanquish system.For example, the interconnection between autosampler and detectorautomatically enables direct synchronization of sample inject and dataacquisition start in the detector. As a result, the synchronization improvesthe retention time reproducibility.

6 Digital I/O ports (Dig I/O )Allow exchange of digital signals with external instrumentsEach digital I/O port provides one input and one relay output. Forconnection and pin assignment information, see Digital I/O ( page 166).

7 USB hub ("A"-type connector) Allows connection to other modules in the Vanquish system

8 USB (Universal Serial Bus) port ("B" type connector)Allows connection to other modules in the Vanquish system or thecomputer on which the data management system is installed, such as theChromeleon software

TIP Thermo Fisher Scientific recommends using the USB ports only asdescribed above. If the USB ports are used for any other purpose,Thermo Fisher Scientific cannot ensure proper functionality.

Follow these steps

NOTICE

• Never use defective communication cables. If you suspect that acable is defective, replace the cable.

• To ensure trouble-free operation, use only the cables provided byThermo Fisher Scientific for connecting the device.

1. Place the device in the system as required by the systemconfiguration. For details, refer to the Vanquish System OperatingManual.

2. Connect the required interface cables to the device. For informationabout how to connect the device to other modules in the Vanquishsystem or to the chromatography data system computer, refer tothe Vanquish System Operating Manual.

3. Connect the power cord (see Connecting the Power Cord( page 55)).

5 • Installation


5.5.3 Connecting the Power Cord

NOTICE

Condensation in a device can damage the electronics.

• Before connecting the devices to the power line, be sure that nocondensation is present in the devices.

• If you suspect that condensation is present, allow the device towarm up to room temperature slowly. Wait until the condensation iscompletely gone before proceeding.

1. Verify that the power switch on the device is set to OFF.

2. Connect the power cord to the power inlet connector on the device.

3. Connect the free end of the power cord to an appropriate powersource.

5 • Installation


5.6 Installing a VIS Lamp (Tungsten Lamp) (Optional)Follow the instructions below to install the optional VIS lamp.

Parts required

VIS lamp

Preparations

1. Turn off the device.

Follow these steps

Figure 9: VIS lamp

No. Description

1 VIS lamp locating pin

2 VIS lamp

3 VIS lamp attachment screw

4 VIS lamp connector

1. Open the doors.

2. Turn the two screws on the lamp house cover counterclockwise untilthe lamp house cover is loose.Do not remove the screws completely from the lamp house cover.

5 • Installation


Figure 10: Screws on the lamp house cover

3. Remove the lamp house cover.

4. Loosen the 2 screws of the VIS slot protection plate and remove theplate. Keep the VIS slot protection plate for later use.

5. Align the new lamp with the locating pin.When the lamp is in the correct position, push the lamp gently intothe lamp house. The lamp socket must be in a level position with thelamp house.

6. When the lamp is properly seated, tighten the two screws to attachthe lamp to the lamp house.

7. Connect the lamp connector.

8. Mind the routing of the lamp cables.Position the lamp cables as indicated in Figure 9 VIS lamp( page 56) to prevent the cable from being pinched under the lamphouse cover.

9. Install the lamp house cover and fasten the screws on the lamphouse cover.

10. Turn on the device with its main power switch.

11. If you install a VIS lamp for the first time and the detector is operatedwith Chromeleon, activate the lamp in Chromeleon:Only then, the VIS lamp can be ignited from Chromeleon.

a) Right-click the detector in the Instrument ConfigurationManager.

b) Select Properties on the menu.

c) On the Detector page, enable the VIS Lamp check box.

5 • Installation


12. After installing a new lamp, increased noise and strong baselinefluctuations may occur. Before beginning an analysis or performing awavelength validation, allow the new lamp to run until the noise isreduced and the baseline is stable which typically takes 24 hours.

13. Perform a wavelength validation (see Performing a WavelengthValidation and Calibration ( page 119)).

5 • Installation


5.7 Installing a Flow CellThis section describes the installation of the flow cell in the detector.

• To remove the diagnostic cell, see Removing the Diagnostic Cell( page 59).

• To install a flow cell without connection unit, see Installing a FlowCell without Connection Unit ( page 60).

• To install a flow cell with connection unit, see Installing a Flow Cellwith Connection Unit ( page 60).

For instructions on cleaning or removing a flow cell or installing adiagnostic cell, see Flow Cell ( page 114).

NOTICE—Sensitive Flow Cells

Flow cells are highly sensitive to dirt and dust. Observe the followingnotes when installing the flow cell to the detector:

• Hold flow cells by the flow cell handle.

• To avoid damage and/or contamination of the optical block of theflow cell, do not touch the optical block.

• On the rear side of the flow cell, the contact pads for theidentification chip are located. Never touch the contact pads. Avoiddamage to the electronics of the ID chip.

NOTICE—Flow Cell Opening

The optical ports and the contact pad for the identification chip in theflow cell opening are sensitive to electrostatic discharge, contaminationand scratches.

Do not touch any surfaces or optical ports in the flow cell opening.

5.7.1 Removing the Diagnostic Cell

The detector is shipped with a diagnostic cell installed. This sectiondescribes the removal of the diagnostic cell.

Follow these steps

1. Open the doors.

2. Loosen the two screws that hold the diagnostic cell in position.

3. Grasp the diagnostic cell by its handle and pull the diagnostic cell outof the detector.

5 • Installation


TIP Store the flow cell in a clean place for later use in diagnosticprocedures or for transporting the device.

5.7.2 Installing a Flow Cell without Connection Unit

Parts required

Flow cell, as required by the application

Preparations

1. Remove the diagnostic cell. See Removing the Diagnostic Cell( page 59).

2. Unpack the flow cell.

Follow these steps

1. Push the flow cell into the flow cell opening.

2. Tighten the two flow cell screws hand-tight.

3. Set up the flow connections to the flow cell, see Setting Up the FlowConnections ( page 62).

4. Perform wavelength validation, see Performing a WavelengthValidation and Calibration ( page 119).

TIP If the wavelength validation fails, perform a calibration ofwavelengths.

5.7.3 Installing a Flow Cell with Connection Unit

Parts required

Flow cell, as required by the application

Preparations

1. Remove the diagnostic cell. See Removing the Diagnostic Cell( page 59).

2. Unpack the flow cell.

Follow these steps

1. Push the flow cell partly into the flow cell opening.

2. Place the connection unit on the connection unit socket. Take carethat the locating pin is placed in the slot.

5 • Installation


4

2

13

Figure 11: Installing a flow cell with connection unit

No. Description

1 Connection unit

2 Locating pin

3 Connection unit socket

4 Connection unit screw (knurl)

3. Tighten the connection unit screw hand-tight.

4. Push the flow cell completely into the flow cell opening.

5. Tighten the two flow cell screws hand-tight.

6. Set up the flow connections on the flow cell, see Setting Up the FlowConnections ( page 62).

7. Perform wavelength validation, see Performing a WavelengthValidation and Calibration ( page 119).

TIP If the wavelength validation fails, perform a calibration ofwavelengths.

5 • Installation


5.8 Setting Up the Flow Connections

5.8.1 General Information and Guidelines

When setting up flow connections, follow these rules andrecommendations:

Flow connections can be filled with hazardous substances. Observe thewarning messages and precautionary statements presented in SafetyPrecautions ( page 21).

NOTICE

Particulate matter from other system modules and components candeposit in the flow cell and clog it.

• Before you connect the flow cell to the flow path, make sure thatyou thoroughly flush the modules in the system flow path upstreamof the device to waste.

• When you install devices or components to the system, always flushthem to waste before connecting them in the system flow path. Toflush the Vanquish modules, follow the instructions in the VanquishSystem Operating Manual.

5 • Installation


NOTICE

Flow cells are highly sensitive to contamination, clogging and highbackpressures. Even if the pressure exceeds the upper limit for a veryshort time only, the flow cell may be permanently damaged. Observethe following notes when connecting the flow cell to the system flowpath:

• When connecting a component in the flow path after the flow cell,observe the specified backpressure for the flow cell.

• Use only clean Viper capillaries which were provided for the flow celland which have been properly protected by their cap before.

• Use only the waste line which was provided for the flow cell.

• Avoid clogging of the flow cell or waste line.

• Improperly set up flow connections can lead to leaks on the flow cellor even destroy the flow cell. Do not invert the flow cell inlet andoutlet.

• If you install switching valves or fraction collectors downstream ofthe flow cell, operating those under flow may result in pressurespikes that can destroy the flow cell. Ensure that pressure spikes donot exceed the flow cell pressure specification.

Follow these steps

To set up the flow connections and complete the installation of thedevice, follow these steps:

1. Set up the flow connections to the flow cell (see Flow Connectionsto the Flow Cell ( page 68)).

2. Connect the device to the drain system (refer to the VanquishSystem Operating Manual).

For installation instructions, guidelines, and handling recommendations,see Connecting Fittings, Capillaries, and Tubing ( page 66).

5.8.2 Guiding Capillaries and Tubing Through the System

Flow connections between the modules of the Vanquish system areguided through either the tubing chase in the devices or the guide holesor capillary clips of the devices.

Tubing Chase, Tubing Guide, Tubing Bracket

To guide certain tubes and lines (solvent tubing, wash liquid tubing,detector waste line) from the top module to the bottom module in theVanquish system stack, the stackable modules have a tubing chase onthe inside right.

5 • Installation


The tubing chase provides four tubing guides. Each guide can hold up tothree tubes or lines.

In each module, push the tube (or line) into the appropriate guide.

Figure 12: Tubing chase with tubing guides (left: view from inside, right:view from top)

No. Use for

1 Solvent tubing (up to three solvent lines)

2 Solvent tubing (up to three solvent lines)

3 Wash liquid tubing (seal wash, autosampler needle wash)

4 Detector waste line

Tubing brackets are available for holding the tubing in place. Slip thebracket side onto the drain pipe.

Figure 13: Tubing bracket (left), tubing bracket installed (right)

Dual System Arrangements

The number of tubes may exceed the capacity that the tubing guides canhold. In this case, it is recommended to place the solvent lines in thetubing guides and route any additional tubes freely in the tubing chase.

5 • Installation


Guide Holes and Capillary Clips

Guide holes and capillary clips are provided at specific positions on thesystem modules. Route flow connections from one module to the nextmodule in the Vanquish system through the appropriate guide hole orcapillary clip when instructed to do so in the manual.

5.8.3 Installing the Partition Panel Plugs

There are two types of partition panel plugs available in the detectorship kit.

Figure 14: Plugs available for the partition panel

No. Description

1 Plug with slit, for guiding capillaries with small outer diameter, such asuninsulated capillaries.

2 Rotating plug, for guiding capillaries that do not fit in the plug with slit,including insulated capillaries.

Installing the plug with slit

1. On the detector partition panel, push the plug with slit in therequired recess of the partition panel (if not present yet).

2. To secure the plug with slit, insert the nose into the opening of thepartition panel.

Figure 15: Securing the partition panel plug with slit

5 • Installation


3. To secure the capillary, push the capillary into the slit of the plug.

Figure 16: Capillary installed in the plug with slit

Installing the rotating plug

1. On the detector partition panel, push the rotating plug in therequired recess of the partition panel (if not present yet).

2. To open the plug in order to route the capillary through the plug,turn the rotating plug toward the front.

3. To secure the capillary, turn the rotating plug toward the partitionpanel to close the opening of the rotating plug.

Figure 17: Using the rotating plug

5.8.4 Connecting Fittings, Capillaries, and Tubing

The inlet and outlet ports of the flow cells support the following capillaryfittings:

• Viper™

• nanoViper™

• Standard 1/16" HPLC fittings using ferrules (PEEK, SST) or finger-tightfittings (PEEK)

This section provides information about how to connect and handlecapillaries, fittings, and tubing.

5 • Installation


5.8.4.1 General Guidelines

When connecting capillaries and tubing, follow these generalrecommendations:

• Use only the capillaries and tubing (for example, solvent lines orwaste tubing) that are shipped with the product or additional orspare capillaries and tubing as recommended by Thermo FisherScientific.

• The connectors must be free from contaminants. Even minuteparticles may cause damage to the system or lead to invalid testresults.

• Do not install capillaries or tubes that are stressed, nicked, kinked, orotherwise damaged.

• Install capillaries and fittings only at the positions for which they areintended.

5.8.4.2 Connecting Viper Capillaries

This section describes how to connect Viper™ capillaries. All Viper flowconnections in the Vanquish system are designed to be finger-tight.

To connect Viper capillaries with knurls, follow these steps:

NOTICE

• Tighten or loosen Viper capillaries only with your fingers. Do not usetools other than the knurl that comes with the capillary.

• To avoid damage to the capillary or connection, tighten and loosenthe Viper capillaries only when the system pressure is down to zero.

5 • Installation


Figure 18: Viper fitting with knurl

No. Description

1 Knurl

2 Capillary

3 Slot

1. Insert the Viper capillary into the connection port.

2. Tighten the connection by the knurl.

TIP Note the slot in the knurl. For narrow connections, you can easilyremove the knurls from neighboring capillaries through this slot andattach them again later.

3. Check whether the connection leaks. If leakage exists, follow thesteps further down.

Resolving Leakage of Viper Fittings with Knurls

1. Tighten the connection a little more.

2. If leakage continues, remove the capillary.

3. Clean the capillary ends carefully by using a lint-free tissue wettedwith isopropanol.

4. Reinstall the capillary.

5. If the connection continues to leak, install a new Viper capillary.

5.8.5 Flow Connections to the Flow Cell

Connect the inlet capillary and waste line to the flow cell when the flowcell is installed to the device.

5 • Installation


In addition, observe the guidelines for proper connection of the flow cellin General Information and Guidelines ( page 62).

Parts required

• Inlet capillary

• Detector waste lineFor instructions on connecting the waste line, follow the steps inConnecting the Detector Waste Line ( page 71).

Tools required

For the detector waste line: Tubing cutter (optional)

Preparations

TIP For easier guiding of capillaries to the flow cell inlet, remove theright door of the detector, see Replacing the Doors ( page 126).

1. Flush the system modules and capillaries upstream of the device towaste before you connect the flow cell to the system flow path.Refer to the Vanquish System Operating Manual.

2. On the flow cell, remove the plugs from the flow cell inlet andoutlet.

TIP Store the plugs of the flow cell, for example in the flow cellpackaging, to have them easily available when storing or shipping theflow cell.

3. Install the partition panel plugs (if not present yet). See Installing thePartition Panel Plugs ( page 65).

Follow these steps

1. Connect the inlet capillary to the flow cell.

2. Connect the detector waste line to the flow cell.

See also

2 Connecting the Inlet Capillary ( page 70)

2 Connecting the Detector Waste Line ( page 71)

5 • Installation


5.8.5.1 Connecting the Inlet Capillary

Preparations

See Flow Connections to the Flow Cell ( page 68).

Follow these steps

12

3

Figure 19: Connecting the inlet capillary from the column compartment(example)

1. Route the inlet capillary from the column compartment through theguide hole in the device enclosure. Use the guide hole that is next tothe column compartment.

TIP Always keep the capillary connection between the columncompartment and the flow cell as short as possible to minimize peakdispersion (i.e. peak broadening effects due to extra dispersion volume).

2. If the column compartment is located to the right of the detectorRoute the capillary through the bottom recess in the partition panel.Make sure that you use the suitable partition panel plug for thecapillary.

TIP For capillaries with small outer diameter, the plug with slit securesthe capillary properly. For capillaries that do not fit in the plug with slit,such as an insulated inlet capillary, use the rotating plug.

3. Connect the inlet capillary to the flow cell inlet or to the inlet (IN) onthe flow cell connection unit.

5 • Installation


5.8.5.2 Connecting the Detector Waste Line

Preparations

1. See Flow Connections to the Flow Cell ( page 68).

Follow these steps

1

2

3

Figure 20: Connecting the detector waste line

1. Connect the waste line to the flow cell outlet or to the outlet (OUT)on the flow cell connection unit (1).

2. Route the waste line through the top recess (2) in the partitionpanel.

3. Route the waste line through the tubing guides of the systemmodules below the detector to the Vanquish system base (3).

4. On the system base, route the detector waste line through thededicated detector waste outlet to waste and connect the detectorwaste line to the waste container as described in the VanquishSystem Operating Manual.

TIP

The waste line should go straight to the system base and to waste. Makesure that the line is positioned straight in the tubing guides.

5. If you have to cut tubing to length, use a tubing cutter. Make surethat the cut is at right angle to the length of the line and that the cutis not crimped.

5 • Installation


6. Check the waste line over the entire flow path: Make sure that nobending (kink), pinching or squeezing of the waste line is present atany point in the flow path.

5.8.6 Guiding Liquid Leaks to Waste

Leaking liquids of the device are collected in the leak tray, where theyflow off through the chase on the right side of the leak tray to the drainsystem.

For information about how the liquid is discharged to waste through theVanquish drain system, refer to the Vanquish System Operating Manual.

5 • Installation


5.9 Turning On the Device

TIP

Before turning on the power to a Vanquish system module for the firsttime, verify that the chromatography software is installed on the datasystem computer. When the power is turned on, the required USBdrivers are automatically found and the Windows™ operating systemcan detect the device.

To turn on the power to the device, follow these steps:

1. Check that the power button on the front left of the Vanquishsystem base (system power button) is pressed in. If the powerbutton stands out, press the power button to turn on the power onthe system base.


Turn the device off with the main power switch, when instructed to doso, for example, during maintenance. Pressing the system power buttonwill not be sufficient to turn off the power to the device completely.

See also

2 Power On/Off Control ( page 81)

5 • Installation


5.10 Setting Up the Device in the SoftwareThis manual assumes that the chromatography software is alreadyinstalled on the data system computer and a valid license is available.

For more information about setting up the Vanquish system in thesoftware, refer to the Vanquish System Operating Manual.

The Help for the software that you are using provides detailedinformation about the settings on each property page.

6 • Operation


6 OperationThis chapter describes the elements for device control, providesinformation for routine operation and for shutdown.

6 • Operation


6.1 Introduction to this ChapterThe information in this chapter assumes that the initial setup of thedevice has already been completed. If this is not the case, refer to theinstructions in Installation ( page 45).

For a basic description of instrument control and automated sampleanalysis with the Chromeleon software, refer to the Vanquish SystemOperating Manual. Details on control and operation of the device areavailable in the Chromeleon Help.

Software descriptions in this manual refer to Chromeleon 7.Terminology may be different to that of other software versions.

6 • Operation


6.2 Safety Guidelines for OperationWhen operating the device, pay attention to the following safetyguidelines:


CAUTION—Hot Surfaces

Surfaces inside the device may become hot during operation. Touchinghot parts might cause burns.

Allow hot surfaces to cool down before you touch them.

6 • Operation


6.3 Control ElementsThe device is designed to be operated mainly from a computer runningwith the chromatography software.

In addition, the following elements are available on the device:

• KeypadThe keypad buttons allow you to perform certain functions directlyfrom the device.

• Status indicatorsThe LEDs (Light Emitting Diodes) on the status indicator LED bar onthe front side of the device and the STATUS LED on the keypadprovide a quick visual check of the operational status of the device.

6.3.1 Keypad

The keypad inside the device allows you to perform certain functionsdirectly from the device. When you press a button, a short beepconfirms that the function is performed.

When the device is connected in the Chromeleon software, somefunctions may not be available from the keypad (see further down in thissection).

Figure 21: Keypad

STATUS

The STATUS LED provides a quick visual check of the operational statusof the device.

When the doors are closed, the LED bar on the front side indicates theoperational status.

For status details, see Status Indicators ( page 79).

6 • Operation


MUTE ALARM

Beeping alerts you when the device detects a problem, for example,leakage. To turn off the beep for the current alarm, press this button.Eliminate the source for the alarm within 10 minutes. Otherwise,beeping starts again. If the device detects a different problem, beepingalerts you again immediately.

UV

The UV button allows you to turn the UV lamp on and off directly fromthe detector. To turn on the UV lamp, press the UV button.

The LED next to the button indicates the lamp status:

LED Description

Off (dark) The UV lamp is turned off.

Green, flashing The UV lamp ignites.

Green The UV lamp is turned on.

VIS

The VIS button allows you to turn the VIS lamp on and off directly fromthe detector. To turn on the VIS lamp, press the VIS button.

The LED next to the button indicates the lamp status:

LED Description

Off (dark) The VIS lamp is turned off.

Green, flashing The VIS lamp ignites.

Green The VIS lamp is turned on.

When the device is connected in the Chromeleon software

The button functionality is as follows when the device is connected inthe Chromeleon software:

• No injection or sequence or baseline monitoring is running:All functions are available from the keypad.

• An injection or sequence or baseline monitoring is running:The MUTE ALARM function remains available from the keypad,allowing you to turn off the beep for the current alarm.

6.3.2 Status Indicators

The status LED bar on the front side of the device and the STATUS LEDon the inside keypad provide information about the device status.

6 • Operation


LED Bar

The LED bar provides the information when the device is closed. Whenthe device is connected in the Chromeleon software, the LED bar mayprovide less information.

LED Bar Description

Off (dark) The power to the device is turned off.

Dimmed The doors of the device are open.

Yellow, flashing slowly The power to the device is turned on, but the deviceis not connected in the Chromeleon software.

Yellow The device is connected in the Chromeleonsoftware, but the device is not equilibrated.

Green, flashing The device is equilibrating. At least one lamp isigniting.

Green The device is connected and equilibrated, but nodata acquisition is running. At least one lamp isturned on.

Blue, running A data acquisition is running.

Blue An injection or sequence is running.

Red A problem or error has occurred. For the relatedmessage, check the Chromeleon Audit Trail. Forremedial action, see Troubleshooting ( page 137).

STATUS LED

The STATUS LED on the keypad inside the device provides the followinginformation:

STATUS LED Description

Off (dark) The power to the device is turned off.

Green The device is functioning properly.

Red A problem or error has occurred. For the related message, checkthe Chromeleon Audit Trail. For remedial action, seeTroubleshooting ( page 137).

For information about the LEDs that are present next to the UV and VISbuttons on the keypad, see Keypad ( page 78).

6 • Operation


6.4 Power On/Off ControlThe power switch on the device is the main switch for power on/offcontrol. The main power switch is turned on during initial installation ofthe device.

For easier handling, you can use the power button on the front left ofthe Vanquish system base (system power button) for power on/off.


• All modules in the Vanquish system that are connected to thesystem base via system interlink cables are turned on or offsimultaneously when the system power button is pressed.

• When the power is on, the system power button is pressed in. Whenthe power is off, the system power button stands out.

• If the main power switch on a device is off, you cannot turn on thedevice with the system power button.

• To turn off a device completely, you have to turn it off with the mainpower switch on the device. Pressing the system power button willnot be sufficient to turn off the power to the device completely.

6 • Operation


6.5 Preparing the Device for OperationThis section gives information on any additional steps that are requiredto prepare the device for operation and sample analysis.

Before Operating the Device for the First Time

Prepare the device for the first-time operation, observing the following:

NOTICE

Flush the system flow path thoroughly before operating the device forthe first time:

• When you install devices or components to the system, always flushthem to waste before connecting them in the system flow path. Toflush the Vanquish modules, follow the instructions in the VanquishSystem Operating Manual.

• When operating the detector with a flow cell that was stored, theflow cell may be filled with solvent. Use solvents that are misciblewith this solvent, or use an appropriate intermediate solvent. Newflow cells are dry or filled with isopropanol when shipped.

• To remove the isopropanol from the device components:

¨ Verify that the flow cell and the column are not connected in theflow path.

¨ Purge the pump and then have the pump deliver flow for a shorttime.

• Verify that any air bubbles are completely flushed out of the systemflow path.

• Perform a wavelength validation. If the validation fails, perform awavelength calibration.

Before Starting Sample Analysis

Before starting an analysis:

• Check the liquid level in the solvent reservoirs. Verify that theamount of solvent is sufficient for the analysis.

• Verify that the doors of all modules in the Vanquish system areclosed.

• Observe the guidelines for use of flow cells in Guidelines for Use ofFlow Cells ( page 84).

• Make sure that the chromatography system is properly equilibrated(see further down).

6 • Operation


System Equilibration

System equilibration should include the following operations:

• Purging the pump (all channels, including those not used for theapplication)

• Flushing the entire chromatography system with the starting solventto rinse out any solvent from a previous analysis run

• Warming up (or cooling down) all temperature-controlled devices inthe system to the starting temperature. Temperature-controlleddevices can be, for example

¨ Column compartment and post-column cooler

¨ Sample compartment thermostatting in the autosampler

¨ Flow cell in a fluorescence detector

¨ Evaporation tube in a charged aerosol detector

• Turning on the lamp (or lamps) in the UV/VIS detector

• Monitoring the pump pressure and pressure ripple and checkingthat the pressure is stable and the ripple within reasonable limits forthe application

• Monitoring the detector signal and checking whether the detectorsignal is stable so that the drift and signal noise are withinreasonable limits for the application

• Performing an autozero of the detector baseline

TIP The Chromeleon software supports procedures for automaticallystarting a chromatography system in the software (Smart Startup). Thestartup procedure includes the operations for system equilibration. Fordetails, refer to the Chromeleon Help.

TIP When you start the data acquisition or perform an autozero, theshutter in the light path before the flow cell is opened automatically.After the data acquisition, close the shutter, if required.

6 • Operation


6.5.1 Guidelines for Use of Flow Cells

NOTICE

Flow cells are sensitive to damage and contamination.

• Handle flow cells with care. See Guidelines for Handling Flow Cells( page 115).

• Observe the guidelines below when operating the detector.

Operating conditions

Observe the specified maximum pressure limit for the flow cell. See thespecifications for flow cells in Flow Cells ( page 152).

Interrupted operation

If the pump flow is interrupted, take appropriate measures to protectthe flow cell:

• Turn off the UV lamp or close the shutter to protect the flow cellfrom the light of the UV lamp. UV radiation from the lamp maydamage the flow cell.

• Never leave any substances in the flow cell without flow. Particularlyavoid leaving any aggressive solvents in the flow cell for a longertime.

• Without flow, air bubbles in the flow cell can accelerate thedeposition of substances. Never leave air bubbles in the flow cell. Ifthe pump flow is stopped for a longer time, fill the flow cell with ahigh purity solvent, for example isopropanol.

Troubleshooting flow cells

• If a flow cell leaks, stop the pump flow, remove the flow cell fromthe detector as quickly as possible, and replace the flow cell.

• For information about how to clean a flow cell, see Cleaning theFlow Cell ( page 115).

Storage and shipment

• Always keep unused flow cells in their original dust-free packaging.

• When shipping the instrument, remove the flow cell and install thediagnostic cell to protect the flow cell opening. The flow cell must beshipped in its original packaging.

See also

2 Removing the Flow Cell ( page 116)

2 Installing a Diagnostic Cell ( page 118)

6 • Operation


6.5.2 Turning On the Lamps

You can turn on both lamps using the UV and VIS buttons on the keypadof the device or using the Chromeleon software. It is recommended toturn on the UV lamp prior to the VIS lamp.

TIP To ignite the lamps, the lamp house cover must be installed. If youremove the lamp house cover while either one of the lamps, or bothlamps are turned on, an emergency shut-off of the lamps is triggered.

Allow the optics to warm up and stabilize for at least one hour until theworking temperature is reached before you start analysis. The opticsrequires some time to warm up for optimal performance beforeanalysis.

TIP When the UV lamp was turned off, a cooling-down period of 5minutes is required before the lamp can ignite again. The detectormonitors the cooling-down period. If the lamp is turned on again afterless than 5 minutes, a message appears in the Chromeleon Audit Trailfollowed by a countdown until the lamp is ignited.

It is not possible to turn any of the lamps off or on during dataacquisition.

6 • Operation


6.6 Operational Modes of the Device

6.6.1 Single-Channel Mode

Single-channel mode is the default mode of operation for the detector.As this mode provides the highest possible data rates and best signal-to-noise ratio, always use the single-channel mode for routine operation ofthe detector.

Consider the following for operating the detector in single-channelmode:

• Sample components should absorb strongly at the selectedwavelength. Therefore, select a wavelength on an absorptionmaximum.

• At the selected wavelength no other sample components shouldabsorb that can falsify the peaks of the sample components ofinterest.

• If the absorbance spectra of the sample components differsignificantly, the measurement wavelength can be switched anytime and as often as required during an acquisition.

• The switching is considerably shorter than one second and should beperformed when no sample components of interest are present inthe flow cell.While the wavelength is switched, no absorption measurementtakes place for a short period of time. If the wavelength is switchedduring an analysis of a sample component, the peak area changes,and a correct quantification is not possible. You can choose betweenthree different baseline behaviors, when switching the wavelength(for details, see Baseline Behavior ( page 93)).

6.6.2 Multi-Channel Mode

The detector provides a multi-channel mode that offers additionaloperation functions for method development and peak-purity analyses.In multi-channel mode, data can be recorded on more than one channelsimultaneously with different, selectable wavelengths. The detectorquickly switches between the selected wavelengths. The maximumnumber of channels depends on your detector variant.

A single analysis is sufficient to measure several channels. Samplecomponents with different absorption characteristics can be analyzedeven without complete chromatographic resolution.

6 • Operation


Consider the following, when operating the detector in multi-wavelength mode:

• Each additional channel reduces the maximum possible datacollection rate, and leads to an increased baseline noise.

• Switching the optical grating permanently between the wavelengthscan lead to wear on the mechanical parts of the detector in the longterm. The multi-channel operation mode is therefore onlyrecommended for method development and peak-purity analysis,but not for continuous routine operation.

• As an alternative, you can switch the wavelengths as often asrequired in a single data channel in the Chromeleon software.For further information on wavelength switching and single-wavelength operation, see Single-Channel Mode ( page 86), andthe Chromeleon Help.

TIP The optical filters for eliminating higher-order wavelengths aremoved automatically into the light path. This applies for multi-channelmode, too.

6 • Operation


6.7 Important Operating ParametersIf a parameter listed below is not available in the Chromeleon software,consider updating the firmware and Chromeleon version.

TIP The Instrument Method Wizard provides different parameter viewmodes. Depending on the parameters to be set, you can select thedesired view mode (for example, Easy or Advanced).

For more information, refer to Chromeleon Help and documents.

Parameters Description

Data collection rate Sets the number of data points per second (Hz) that theChromeleon software collects from the detector andstores as raw data. The maximum data collection ratedepends on the detector type and the software version.For more information on how to set the data collectionrate, see Data Collection Rate ( page 92).

Lamphousetemperature

The temperature signal channel (LamphouseTemperature) that is available in the Properties dialogbox of the detector, can be used for troubleshootingpurposes.The lamps of the detector are very sensitive to changes intemperature. In case of baseline drift problems, the lamp-house temperature signal can help to find the reason forthe problem.Select the signal channel to record the temperature.Chromeleon generates the appropriate channel forrecording the temperature of the detector lamp house.

Leak detection Leak detection is enabled as a standard when thedetector is shipped (Leak Sensor Mode = Enabled). This isthe preferred setting.

Peak width The peak width is a supporting parameter for the datacollection rate setting and the response time.For details on the peak width and selecting it individually,see Response Time and Peak Width ( page 92).

Response time The response time determines how quickly the detectorresponds to a change in signal.For details on the response time and selecting itindividually, see Response Time and Peak Width( page 92).

6 • Operation


Parameters Description

Spectra scanning To record the absorbance spectra of substances that areused in an analysis, for example, to detect peakimpurities, or to identify optimum wavelengths formethod development, perform spectra scanning:ScanStartWavelength sets the wavelength at which thescan should start.ScanEndWavelength sets the wavelength at which thescan should end.ScanSpeed sets the speed at which the wavelength rangeis scanned.ScanBaseline saves a baseline scan based on the currentsettings.Scan saves an absorbance spectrum based on the currentsettings.Note: Before you can record an absorbance spectrum,perform a ScanBasline with the same wavelength settingsthat you want to use for recording the absorbancespectrum.For further information about the parameters andcommands, refer to the Chromeleon Help.

UV lampVIS lamp

Turn on the UV and/or VIS lamp before starting ananalysis (UV_Lamp = On, Visible_Lamp =On). This settingallows turning on and off the UV and VIS lamp of thedetector.To turn the UV lamp on automatically after power on, setAutoactivateUV_Lamp to On.To turn the VIS lamp on automatically after power on, setAutoactivateVisible_Lamp to On.

UV_VIS The UV_VIS signal channels (UV_VIS_x) that are availablefor data acquisition are listed in the dialog box for thedetector in the Instrument Configuration Manager. Selectthe required signal channels.Recording at more than one signal channel, thus at morethan one wavelength, sets the detector into multi-channelmode. For details, see Multi-Channel Mode ( page 86).

Wavelength Sets the wavelength in nm. Select the wavelength at theabsorption maximum of the sample component to beanalyzed.

Wavelength calibration If the wavelength validation failed, perform a calibrationof wavelengths.The 0-order radiation, which corresponds to 000 nm ofthe wavelength scale, is used to calibrate the wavelength.For further information, see Performing a WavelengthValidation and Calibration ( page 119).

Wavelength validation Perform the wavelength validation as required. Aholmium-oxide glass filter is moved into the light path ofthe lamp and is used to validate the wavelength accuracy.For further information, see Performing a WavelengthValidation and Calibration ( page 119).

6 • Operation


6.8 Optimizing the Performance of the DeviceThis section provides information for best performance of the deviceand gives hints on what you can do to optimize the performance further.

6.8.1 General Guidelines

Consider the following guidelines for optimization of the deviceperformance:

• Do not turn off the lamp or lamps during short measurement breaks.Igniting a lamp frequently reduces the lifetime of the lamp. Inaddition, some time is required for the detector to stabilize after re-ignition of a lamp.

• Degas the solvent.

• Consider the influences of the parameters on the detection.

• Keep the device doors closed during operation to avoid exposure ofthe flow cell to thermal changes in the environment that may lead toa baseline drift.

• Ensure that the operating conditions are suitable. This includes:

¨ Stable environmental conditions, such as a stable temperature

¨ No air drafts

¨ No vibrations or mechanical shocks caused by external sources

¨ Stable backpressure and correct waste line setup

• For information about the UV cutoff wavelengths of solvents, see UVCutoff Wavelengths of Solvents ( page 165).

• Monitor the usage of specific components that are subject to wearand stress and schedule appropriate maintenance intervals (seePredictive Performance ( page 106)).

• Observe the general guidelines and recommendations on the use ofsolvents and additives in the chromatography system. Refer to Useof Solvents and Additives in the Vanquish System Operating Manual.

See also

2 Overview of Optimization Parameters ( page 91)

2 UV Cutoff Wavelengths of Solvents ( page 165)

6 • Operation


6.8.2 Overview of Optimization Parameters

The following table serves as an overview of parameters that influencethe detector performance and indicates the performance characteristicsaffected.

Parameter Affects

Wavelength Sensitivity, linearity

Data collection rate Peak resolution, disk space

Response Time/Peak width Baseline noise, peak width, chromatographicresolution, sensitivity

Baseline behavior Behavior of the baseline after switching thewavelength

For further information about the parameters, refer to the ChromeleonHelp.

6.8.3 Wavelength

The most important parameter that needs to be optimized is thewavelength. Note the following key criteria for determining thewavelength for an analysis:

• Preferably, select a wavelength on the absorption maximum of thesample components.

• Avoid the wavelength range where the solvents absorb strongly (forexample, below 220 nm for methanol and below 210 nm foracetonitrile). The wavelength should always be selected above theUV cutoff of the solvent. For information about the UV cutoffwavelengths of solvents, see UV Cutoff Wavelengths of Solvents( page 165).

Wavelength switching in the Chromeleon software

Thermo Fisher Scientific recommends using the single-channel mode torecord data by switching the wavelengths in-between the detectedpeaks of the various sample components, rather than to simultaneouslymeasure all wavelengths in multi-channel mode.

Use the Instrument Method Wizard to set the start wavelength for onechannel. After completion of the wizard, open the method in theInstrument Method Editor. In the Module View for the detector, on theTimetable tab page, you can define the times for switching thewavelengths and other parameters.

6 • Operation


6.8.4 Data Collection Rate

The data collection rate is the number of data points per second (Hz)that the Chromeleon software collects from the detector and stores asraw data.

When you select a data collection rate, observe the following guidelines:

• In general, each peak should be defined by at least 20 data points.For chromatograms with co-eluting peaks or low signal-to-noiseratios, 40 data points per peak are recommended.

• If the data collection rate is too low, the start points, maxima, andend points of peaks will not be determined accurately.

• If the data collection rate is too high, data files may need more diskspace and post-run analyses may require more processing time.

• If all peaks are relatively wide, select a lower data collection rate (forexample, 1.0 Hz). This saves disk space and allows for a faster displayof data in the Chromeleon software.

• If any peaks of interest are less than a few seconds, select a higherdata collection rate (10.0 Hz, for example).

Multiple wavelength applications

In multiple wavelength applications, baseline noise increases comparedto single-channel data acquisition. This is especially the case if the datarate is set to the maximum or close to the maximum. To improve thesignal-to-noise ratio, select a lower data collection rate.

6.8.5 Response Time and Peak Width

As a standard, when setting the data collection rate, Chromeleonautomatically sets the optimum response time and peak width. As anadvanced option, response time and peak width can be set individually.

TIP

Disabling the link between the data collection rate parameters andsetting these parameters individually may lead to increased noise orincreased peak widths.

Observe the following guidelines when changing the response time andpeak width proposed by Chromeleon:

• Ensure that response time and peak width match the setting for thedata collection rate.

6 • Operation


• The response time should be about 30% of the peak width at half-height of the narrowest peak of interest.

• A longer response time allows more averaging of the signal andresults in less short-term noise. However, if the selected responsetime is too long, this can result in reduced peak heights andasymmetrical peak shapes. If a separation of peaks is done thatfollow closely to each other, the long response time can result in badpeak separation. When set correctly, the response time significantlyreduces baseline noise, and reduces peak height only slightly.

TIP For best possible combinations of data collection rate, response timeand peak width, enable the Link data collection parameters check box inthe detector settings of the Chromeleon Instrument Method Wizard orInstrument Method Editor.

6.8.6 Baseline Behavior

If wavelengths are switched during a single-channel data acquisition, thechromatogram may show baseline jumps.

You can define how the baseline behaves in these cases:

• Zero: sets the baseline to zero

• Append: appends the baseline to the previous signal

• Free: lets the baseline "jump" to the current absolute value

TIP You can perform the AutoZero command (automatic null balancing)any time during data acquisition, that is, the absorption measured at thetime of the autozero is subtracted from any absorptions measuredthereafter. Always select a time for AutoZero at which no sample flowsthrough the cell.

6 • Operation


6.9 Shutting Down the DeviceIf the device will not be operated for some time, follow the instructionsin this section to shut down the device.

TIP The Chromeleon software provides procedures for automaticallypreparing the chromatography system for shutdown. The proceduresinclude, for example, operations for reducing the flow rate, reducing thetemperature in temperature-controlled devices, and turning off thedetector lamps. For information about Smart Shutdown and SmartStandby, refer to the Chromeleon Help.

6.9.1 Short-Term Shutdown (Interruption of Operation)

To interrupt operation of the device for a short period (short-termshutdown), for example, overnight, observe these guidelines for theVanquish system modules, as required by your system arrangement:

• For your Vanquish detector, note the following:

Detector Type Description

Charged aerosoldetector

Check that sufficient gas is available to continue gas flowingthrough the detector. This is to prevent any build-up ofresidue from solvents or analytes. Gas must be flowing whenpump flow is delivered to the detector.

UV/VIS detectors The lamp(s) in the detector can remain turned on.Variable wavelength detector and VH-D10 diode arraydetector only: The shutter can be moved to a closed positionfor protection of the flow cell.

Fluorescencedetector

Turn off temperature control for the flow cell.

• Apply a flow of 0.05 mL/min and have the pump deliver anappropriate solvent.Check the lower pressure limit for the pump and adapt the value ifnecessary. If the pressure falls below the lower limit, the pump stopsthe flow.

• Set the injection valve in the autosampler to the Inject position.

• Make sure that the temperature of the column does not exceed40 °C.

• When resuming operation, let the flow equilibrate and verify thatthe operating parameters for the other system modules are set asrequired before proceeding.

6 • Operation


6.9.2 Long-Term Shutdown

Shutting Down the Device

To interrupt operation for a longer period, follow the instructions below.

TIP Shutting down the device affects the operation of the system. Whenshutting down the device, also observe the shutting down instructionsfor the other Vanquish system modules and take appropriate action(refer to the Operating Manuals for the modules).

1. Turn off both lamps.

2. Stop the pump flow.

3. Remove the column.

4. Connect the free ends of the column compartment capillaries usinga union connector (for example, the Viper union from the systemship kit) and restart the pump flow.

5. Flush the flow cell with an appropriate solvent (minimum HPLC-grade). Observe the following:

TIP With a Vanquish Core system that has been modified for usingnormal-phase compatible solvents and additives, see the informationabout the flushing liquid in the Considerations with Normal-PhaseCompatible Solvents and Additives section in Operating Manual for thepump.

Situation after Shutdown If no additive is used If an additive is used

Device and flow cellremain in the laboratoryafter shutdown

Flush the system, forexample with methanol.100% acetonitrile shouldnot be used.

Flush the system withseveral volumes ofmethanol and water(50:50) (for example,1.0 mL/min for10 minutes with thestandard system) toprevent salt buildup in thefluidics. If the solvents inthe flow cell are notmiscible with water, usean appropriateintermediate solvent.

6 • Operation


Situation after Shutdown If no additive is used If an additive is used

Device and flow cell shallbe transported or shippedafter shutdown

Flush the system withisopropanol.

Flush the system first withseveral volumes ofmethanol and water(50:50) (for example,1.0 mL/min for10 minutes with thestandard system) toprevent salt buildup in thefluidics. If the solvents inthe flow cell are notmiscible with water, usean appropriateintermediate solvent.Afterward, flush thesystem with isopropanol.

NOTICE

Residual samples, impurities from the column or buffers with high saltconcentrations can deposit in the flow cell. This can lead to damage ofthe flow cell. In addition, solvents containing acid can damage the flowcell.

• Always flush the flow cell with an appropriate solvent beforeinterrupting operation.

• Fill the flow cell with pure isopropanol using the flushing andinjection kit.

• The flow cell should not be filled with pure water to avoid thegrowth of algae. If you want to fill the flow cell with water, you needto add 10% HPLC-grade isopropanol.

6. Turn off the pump flow to the device. Wait until the system pressureis down to zero before you continue the shutdown of the device.

7. Disconnect the capillaries from the flow cell inlet and outlet.

8. After removing the flow connections, protect the flow cell inlet andoutlet ports with the plugs supplied with the flow cell. Protect theinlet capillaries with caps.

9. The step depends as follows:

Situation Steps

Device and all other system modulesremain in the system stack and are to beturned off

Turn off the system with the systempower button on the system base.

6 • Operation


Situation Steps

Device shall be transported or shippedafter shutdown

If one of the modules shall be removedfrom the system stack, turn off allsystem modules with their main powerswitch. Pressing the system powerbutton will not be sufficient to turn offthe power to the devices completely.Follow the instructions in Transporting orShipping the Device ( page 128).

See also

2 Connecting the Inlet Capillary ( page 70)

2 Connecting the Detector Waste Line ( page 71)

6 • Operation


7 • Maintenance and Service


7 Maintenance andServiceThis chapter describes the routine maintenance and the serviceprocedures that the user may perform.



7.1 Introduction to Maintenance and ServiceThis chapter describes the routine maintenance and service and repairprocedures that the user may perform.

Additional maintenance or service procedures must be performed onlyby service personnel certified by Thermo Fisher Scientific (for brevity,referred to as Thermo Fisher Scientific service personnel).

The device is designed for easy maintenance and service. The user-serviceable parts of the device can be accessed from the front. If notstated otherwise, the maintenance procedures do not require that youremove the device from the system.

The maintenance procedures do not require that you remove the doors.However, it is possible to remove a door if this should ever be requiredfor a specific reason or procedure. If you need to remove a door, followthe related steps in Replacing the Doors ( page 126).



7.2 Safety Guidelines for Maintenance and ServiceWhen performing maintenance or service procedures, pay attention tothe following safety guidelines:


WARNING—High Voltage

High voltages are present inside the device that could cause an electricshock.

Do not open the housing or remove protective panels unless specificallyinstructed to do so in this manual.

WARNING—Escape of Hazardous Substances from Flow Connections

Flow and capillary connections can be filled with substances that canpose health risks. Solvent can spray when capillaries burst, slip out oftheir fittings, or are not properly tightened or when capillaryconnections are otherwise open.

• Wear appropriate protective equipment and follow good laboratorypractice.

• Before starting maintenance or repair procedures, flush out harmfulsubstances with an appropriate solvent.

WARNING—Tilting Liquid Reservoirs

Liquids in the reservoirs on the solvent rack might contain harmfulsubstances. Spilling of these substances can pose health and safety risks.

To prevent the reservoirs from tilting, be careful not to pull on the liquidlines when performing maintenance.

CAUTION—Spraying Solvent

Solvents can spray when under high pressure.

• Stop the pump flow prior to opening the flow path.

• Wait until the system pressure is down to zero.

• When opening the flow path, wear appropriate protectiveequipment.



CAUTION—Hot Surfaces

Surfaces inside the device may become hot during operation. Touchinghot parts might cause burns.

Allow hot surfaces to cool down before starting replacement ormaintenance procedures.

CAUTION—Hydrostatic Pressure

Solvent may spill when you open the flow path. This is due to hydrostaticpressure in the system when the solvent reservoirs are located abovethe pump outlet. Before you loosen a connection in the flow path:

• Turn off the pump flow and wait until the system pressure is downto zero.

• Unscrew the caps of the solvent reservoirs and remove the solventlines together with the caps from the reservoirs.

• Empty the solvent lines. Refer to the Operating Manual for thepump.

• Retighten the reservoir caps.


After the power to the device is turned off, the device is still energizedas long as the power cord is connected. Repair work on the device whilethe device is connected to power could lead to personal injury.

• Always unplug the power cord before starting repair work inside thedevice.

• If you were instructed to remove any housing covers or panels, donot connect the power cord to the device while the cover or panelsare removed.



7.3 General Rules for Maintenance and ServiceFor successful maintenance and service procedures, follow these rulesand recommendations:

• Before starting maintenance or service procedures, shut down thedevice when instructed to do so.

• Use only the replacement parts specifically authorized and qualifiedfor the device by Thermo Fisher Scientific.

• Follow all instructions step by step and use the tools recommendedfor the procedure.

• Before opening the flow path to replace capillaries in the system,turn off the pump flow and wait until the system pressure is down tozero.

• Dirty components can contaminate the chromatography system.Contamination leads to poor performance of the modules and entiresystem or can even cause damage to the modules and system.Therefore:

¨ Always wear appropriate gloves.

¨ Place the components only on a clean, lint-free surface.

¨ Keep your tools clean.

¨ Use only lint-free cloth for cleaning.

• If you need to return the device for depot repair, follow theinstructions in Transporting or Shipping the Device ( page 128).

See also

2 Consumables and Replacement Parts ( page 159)



7.4 Routine and Preventive MaintenanceOptimum device performance, maximum uptime of the device, andaccurate results can be obtained only if the device is in good conditionand properly maintained.

7.4.1 Maintenance Plan

Perform the maintenance procedures in the table on a regular basis. Thefrequency given in the table is a suggestion. The optimum frequency formaintenance depends on several factors, such as the types and amountsof samples and solvents used with the device.

Frequency What you should do...

Daily • Inspect the flow connections for signs of leakage orblockage.

• When you use buffers or salt solutions, flush the devicethoroughly after use with an appropriate solvent thatdoes not contain buffers or salts. Monitor thebackpressure that the flow cell is exposed to.

Regularly • Inspect the flow connections for damage, such as cracks,nicks, cuts, or blockage.

• Check that all warning labels are still present on thedevice and clearly legible. If they are not, contact ThermoFisher Scientific for replacement.

Annually Have Thermo Fisher Scientific service personnel performpreventive maintenance once a year.

TIP The Chromeleon software supports functions for monitoring andrecording service and qualification information (see Monitoring the lampage).

7.4.2 Cleaning or Decontaminating the Device

Cleaning and decontamination must be performed by qualifiedpersonnel wearing suitable personal protective equipment. Alwaysobserve national and local regulations.

NOTICE

Wipe up all liquids spilled onto the system immediately. If surfaces areexposed for longer periods, these liquids can cause damage.



Decontamination

Decontamination is required, for example, when leakage or spillage hasoccurred, or before service or transport of the device. Use a suitablecleaning detergent or disinfectant to ensure that the treatment rendersthe device safe to handle.

Parts required

• Suitable cleaning detergent (or disinfectant)

• Purified water

• Lint-free cloths or wipes

CAUTION—Explosive Gas Mixtures from Alcoholic Cleaning Detergents

Alcohol-containing cleaning detergents may form flammable andexplosive gas mixtures when exposed to air.

• Use such cleaning detergents only when required and only inadequately ventilated rooms.

• Avoid open flames or exposure to excessive heat during the cleaningprocess.

• Wipe the cleaned components thoroughly dry after cleaning. Do notoperate the device before it is completely dry.

NOTICE


• Only use cleaning detergents that will not damage the surfaces ofthe system.

• Never use sharp tools or brushes for cleaning any surfaces.

• Do not use sprays for cleaning.

• Prevent cleaning detergent from entering the flow path.

• Do not use excessively wetted cloth or wipes for cleaning. Preventany liquids from entering the functional components of the device.Liquids can cause a short circuit when getting in contact with theelectronic components.

NOTICE—Flow Cell Opening

The optical ports and the contact pad for the identification chip in theflow cell opening are sensitive to electrostatic discharge, contaminationand scratches.

Do not touch any surfaces or optical ports in the flow cell opening.



Preparations

1. Turn off the power to the device and disconnect the power cordfrom the power source.

Follow these steps

1. Wipe the surfaces clean with a clean, dry, soft, lint-free cloth orwipe. If necessary, slightly dampen the cloth or wipe with a solutionof lukewarm water and a suitable cleaning detergent.

2. Allow the cleaning detergent to react as recommended by themanufacturer.

3. Wipe the cleaned surfaces with purified water to ensure that allcleaning detergent residues have been removed.

4. Wipe the surfaces dry using a soft, lint-free cloth or wipe.

7.4.3 Predictive Performance

General overview

The Chromeleon software supports functions for estimating the lifetimeof consumables and for monitoring and recording service andqualification information about the device. These functions are calledPredictive Performance. They allow you to schedule maintenanceprocedures based on the actual operating and usage conditions of thedevice.

On special wellness, service, and qualification panels, you can defineintervals for replacing components that are subject to wear or stress andfor service procedures or qualification procedures. In addition, you canset limits to alert you before and when the replacement, service, orqualification is due.

Color-coded bars on special panels provide visual feedback, allowing youto easily check and monitor the status. If a warning limit was set, amessage in the Chromeleon Audit Trail alerts you when the action isdue.

Some counters can be reset to zero after the required action wasperformed. To keep the Predictive Performance information up-to-date,consider resetting the counter when a maintenance, service, orqualification procedure has been performed.

For more information, refer to the Chromeleon Help.



7.5 LampsThis section describes the replacement and installation of the

• UV lamp (see Replacing the UV Lamp (Deuterium Lamp)( page 107)), and

• VIS lamp (see Replacing the VIS Lamp (Tungsten Lamp)( page 110)).

NOTICE—Damage to the device

Usage of lamps not supplied or approved by Thermo Fisher Scientific forthis detector may result in inferior performance, detector malfunction ormay damage the detector. Detector guaranty may be voided.

Use only lamps supplied or approved by Thermo Fisher Scientific for thisdetector.

7.5.1 Replacing the UV Lamp (Deuterium Lamp)

When

• The lamp is defective

• After a defined amount of operating hours of the lamp as apreventive measure

• The lamp intensity is too low for demanding applications

• The lamp intensity fluctuates causing artifacts in the baseline

Parts required

• UV lamp

• Optionally:

¨ Isopropanol

¨ Lint-free tissue

Preparations

1. Turn off the UV lamp.




Follow these steps

Figure 22: UV lamp

No. Description

1 UV lamp connector

2 UV lamp

3 UV lamp attachment screw

4 UV lamp locating pin

1. Open the doors.






CAUTION—Hot surface

The lamp may become hot. Touching a hot lamp might cause burns.

• Touch the lamp briefly and carefully to find out if it is hot before youremove the lamp.

• If it is hot, wait until the lamp has cooled down.

4. Push the clip on the lamp connector and disconnect the connectorfrom the lamp socket on the device.

5. With your hands, loosen the two screws that attach the lamp to thelamp house.

6. Pull out the UV lamp.

7. Inspect the new UV lamp for signs of fingerprints and dust.If necessary, clean the UV lamp with isopropanol and a lint-freetissue before you install it.



10. Reconnect the lamp connector.



11. Mind the routing of the lamp cables.Position the lamp cables as indicated in the figure above to preventthe cable from being pinched under the lamp house cover.

12. Reinstall the lamp house cover and fasten the screws on the lamphouse cover.


14. After replacing the lamp, increased noise and strong baselinefluctuations may occur. Before beginning an analysis or performing awavelength validation, allow the new lamp to run until the noise isreduced and the baseline is stable which typically takes 24 hours.


TIP

The lamp age counter is automatically reset to the value stored on the IDchip of the lamp.

See also

2 Performing a Wavelength Validation and Calibration ( page 119)

7.5.2 Replacing the VIS Lamp (Tungsten Lamp)

When

• The lamp is defective

• After a defined amount of operating hours of the lamp as apreventive measure

• The lamp intensity is too low for demanding applications

• The lamp intensity fluctuates causing artifacts in the baseline

Parts required

VIS lamp

Preparations

1. Turn off the VIS lamp.




Follow these steps

Figure 24: VIS lamp

No. Description

1 VIS lamp locating pin

2 VIS lamp

3 VIS lamp attachment screw

4 VIS lamp connector

1. Open the doors.






CAUTION—Hot surface

The lamp may become hot. Touching a hot lamp might cause burns.

• Touch the lamp briefly and carefully to find out if it is hot before youremove the lamp.

• If it is hot, wait until the lamp has cooled down.

4. Push the clip on the lamp connector and disconnect the connectorfrom the lamp socket on the device.

5. With your hands, loosen the two screws that attach the lamp.

6. Pull out the lamp.



9. Connect the lamp connector.

10. Mind the routing of the lamp cables.Position the lamp cables as indicated in Figure 24 VIS lamp( page 111) to prevent the cable from being pinched under thelamp house cover.



11. Install the lamp house cover and fasten the screws on the lamphouse cover.


13. If you install a VIS lamp for the first time and the detector is operatedwith Chromeleon, activate the lamp in Chromeleon:Only then, the VIS lamp can be ignited from Chromeleon.

a) Right-click the detector in the Instrument ConfigurationManager.

b) Select Properties on the menu.

c) On the Detector page, enable the VIS Lamp check box.

14. After replacing the lamp, increased noise and strong baselinefluctuations may occur. Before beginning an analysis or performing awavelength validation, allow the new lamp to run until the noise isreduced and the baseline is stable which typically takes 24 hours.


TIP

The lamp age counter is automatically reset to the value stored on the IDchip of the lamp.

See also

2 Turning On the Lamps ( page 85)



7.6 Flow CellThis section describes cleaning, replacement, storage and shipment offlow cells.

To store or ship the flow cell, follow these steps:

1. Flush the flow cell with isopropanol.

2. Remove the flow cell from the detector. See Removing the Flow Cell( page 116).

3. Install the plugs on the flow cell inlet (IN) and outlet (OUT), andplace the flow cell in its packaging.

To exchange the flow cell, follow these steps:

1. Flush the flow cell with isopropanol.

2. Remove the flow cell from the detector. See Removing the Flow Cell( page 116).

3. Install a new flow cell without connection unit to the detector. SeeInstalling a Flow Cell without Connection Unit ( page 60).– or –

4. Install a new flow cell with connection unit to the detector. SeeInstalling a Flow Cell with Connection Unit ( page 60).



7.6.1 Guidelines for Handling Flow Cells

NOTICE

Flow cells are highly sensitive to dirt and dust. Observe the followingnotes when handling flow cells:

• When holding flow cells, do not touch the optical block of the flowcell or the sensitive electronics on the flow cell rear side.

• The optical ports of the flow cell are sensitive to contamination andscratches. Do not touch the optical ports of the flow cell or immersethem.

• To avoid damage to the optical ports of the flow cell, be carefulwhen inserting the flow cell into the flow cell opening of the device.

• On the rear side of the flow cell, the contact pads for theidentification chip are located. Never touch the contact pads. Avoiddamage to the electronics of the ID chip.

• Particulate matter, dust and debris can lead to contamination andclogging of the flow cell. If capillaries are removed from the flow cellinlet and outlet, install protective plugs to close the ports and toprevent particles from clogging the flow cell in the next application.Always use the plugs that were installed when the flow cell wasshipped.

• Use the dedicated packaging when storing or transporting the flowcell.

7.6.2 Cleaning the Flow Cell

When

When you suspect that eluent or sample components may havedeposited on the flow cell windows.

Parts required

• Flushing and injection kit for flow cells (optional)

• HPLC-grade water

• 0.1 M nitric acid



WARNING—Health Risk

The handling of solvents can pose health and safety risks.

Wear personal protective equipment as required by the hazard andfollow good laboratory practice. Refer to the material handling andsafety data sheet provided by the vendor.

Follow these steps

You can perform the following procedure by using the optional flushingand injection kit.

1. Flush the flow cell with HPLC-grade water.

2. Fill the flow cell with 0.1 M nitric acid using the optional flushing andinjection kit.

3. Flush the flow cell with HPLC-grade water until the solvent leavingthe flow cell is neutral (pH 7).

4. If cleaning the flow cell does not eliminate the problem, install a newflow cell.

See also

2 Installing a Flow Cell with Connection Unit ( page 60)

2 Installing a Flow Cell without Connection Unit ( page 60)

7.6.3 Removing the Flow Cell

When

• Storing the flow cell in its packaging

• Replacing the flow cell

• Leakage occurred on the flow cell

Parts required

• If the flow cell is to be storedPackaging of the flow cell

• If no flow cell is to be installed to the detector afterwardDiagnostic cell

Preparations

1. If the flow cell is to be storedFlush the flow cell with isopropanol.

2. Turn off the pump flow.



Follow these steps

NOTICE

Do not open the tubing connections between the connection unit andthe flow cell. The tubing connections are installed at the factory.

1. Flow cell without connection unit onlyDisconnect the waste line from the outlet of the flow cell, thendisconnect the inlet capillary from the inlet of the flow cell (if not yetdone).

– or –

Flow cell with connection unit only

Disconnect the waste line from the outlet port (OUT) of the flow cellconnection unit, then disconnect the inlet capillary inlet port (IN) ofthe flow cell connection unit.

2. Loosen the two screws that hold the cell in position.

3. Flow cell with connection unit onlyLoosen the screw that holds the connection unit in position andremove the connection unit from the connection unit socket.

4. Carefully pull out the cell from the opening in the detector.

5. Install the flow cell plugs on the inlet and outlet ports.

6. If the flow cell is to be storedStore the flow cell in its original packaging that it was shipped in.

7. Proceed as required:

¨ Install a flow cell- or -

¨ Install the diagnostic cell to the opening of the detector.

NOTICE

The flow cell opening on the device is sensitive to dust and debris.

If no flow cell is installed to the detector, install a diagnostic cell to theflow cell opening of the detector.

See also






7.6.4 Installing a Diagnostic Cell

Parts required

Diagnostic cell

Follow these steps

1. Push the diagnostic cell into the flow cell opening.

2. Tighten the two screws of the diagnostic cell hand-tight.



7.7 Performing a Wavelength Validation andCalibration

A holmium-oxide glass filter is used to validate the wavelength accuracy.The filter can be moved into the light path in front of the entrance slitfor the wavelength validation. The detector determines the wavelengthsof several absorption peaks in the resulting spectrum and comparesthem to the nominal holmium oxide values that are stored in thedetector firmware.

A validation can be performed for all lamp configurations (both lampsturned on, only UV lamp turned on, and, but least favorable, only VISlamp turned on (if available)).

The accuracy is verified for the wavelengths that are stated in thedeclaration of conformity for the holmium-oxide glass filter. Forinformation about the declaration, see NIST Compliance ( page 163).

A wavelength validation can also be performed with an externalstandard, such as a caffeine solution. In this case, an accuracy of ± 1 nmcan be achieved.

When

A wavelength validation is recommended in the following situations:

• After the flow cell has been exchanged

• After a lamp has been replaced

Preparations

Before you start the wavelength validation, observe the following notes:

• Allow the flow cell to warm up for 5 minutes.

• Ensure that the baseline is sufficiently stable. The baseline maybecome unstable, for example, if the solvent composition has beenmodified, or if air bubbles are present in the light path.

• Verify that the solvent flowing through the flow cell is not stronglyabsorbing in the wavelength range of the holmium-oxide glass filterthat is to be verified.This problem occurs, for example, if the flow cell is filled with amixture of 96% hexane and 4% ethyl acetate. Thermo FisherScientific recommends using degassed LC/MS-grade water.



• Allow the lamps to warm up and reach operating temperature. Thelamps should be running for at least 1 hour before you start thewavelength validation. A lamp spectrum changes significantly duringthe first few minutes after a lamp has been turned on.

To perform a wavelength validation

1. Turn on the flow at the flow rate that will be used for the analysis.

2. In Chromeleon, perform WavelengthValidation.

3. If the wavelength validation fails, perform a wavelength calibrationfrom Chromeleon.

To perform a wavelength calibration

TIP To ensure optimum performance and wavelength accuracy, performWavelengthCalibration in Chromeleon after warm up of the optics.

1. In Chromeleon, select WavelengthCalibration.



7.8 Replacing the Waste LineWhen

Blockage or leakage of one or more waste line components

Parts required

Detector waste line

For instructions on connecting the waste line, follow the steps in thissection.

The waste line is routed through the system base and connected to thewaste. For instructions, refer to the Vanquish System Operating Manual.

Tools required

Tubing cutter (optional)

Preparations

1. Close the shutter in the light path of the flow cell.

2. Stop the pump flow to the flow cell.

Follow these steps

1. When removing the waste line from the tubing guides, be carefulnot to pull on other tubing in the guides.

2. Disconnect the waste line from the flow cell outlet or the outlet(OUT) of the flow cell connection unit.

3. Remove the waste line from the capillary guide of the partitionpanel. Turn the plug in the top capillary guide to the front andremove the waste line from it.

4. Remove the waste line from the detector waste port in the systembase and from waste.

5. Unpack the replacement waste line.

6. Connect the waste line. Follow the instructions for connecting thewaste line in Connecting the Detector Waste Line ( page 71).



7.9 Replacing the Main Power FusesWhen

Blown fuses

Parts required

Fuses (2 fuses, 3.15 AT, 250 V AC, slow-blow, 5 x 20 mm) from Fuses Kit

Tools required

Slotted screwdriver, any size between 3.3 mm and 5.5 mm isappropriate

Preparations

WARNING—Electric Shock

High voltages are present inside the device that could cause an electricshock or damage to the device.

• Turn off the device with its main power switch. Disconnect thepower cord from both the power source and the device.

• Use only the fuses of the type and current rating specified for thedevice by Thermo Fisher Scientific.

• Do not use repaired fuses and do not short-circuit the fuse holders.

Follow these steps

The fuse holder is located next to the main power switch.

Figure 26: Fuse holder

No. Description

1 Main power switch (on/off control)

2 Fuse holder

3 Power-inlet connector



1. Use the screwdriver to remove the fuse holder.

2. Replace the two fuses with new fuses of the specified type andcurrent rating. Always replace both fuses.

3. Reinstall the fuse holder.

4. Reconnect the power cord to the power source and to the device.

5. Turn on the device with the main power switch.



7.10 Updating the Device FirmwareWhen

Updating the device firmware might be required, for example, when anew firmware version is released that adds functionality or solvesproblems of a previous version.

Items required

Firmware version/Chromeleon version as appropriate

TIP When a new firmware version is released, the new version will beincluded in the next available Chromeleon version. The new firmwarewill not be transferred automatically to the device when you install theChromeleon version.

Preparations

1. Read the release notes provided with the firmware and/orChromeleon version.

2. Connect the device in the Chromeleon software.

3. Stop all operations on the Instrument that includes the device.

4. Wait until the Instrument is idle.

Follow these steps

1. Start the Instrument Configuration Manager program.

2. Perform a firmware update from the General tab page in theconfiguration dialog box for the device. For details, refer to theChromeleon Help.The firmware update may take several minutes.

NOTICE

A firmware downgrade or incomplete firmware update may result in lossof functionality or malfunctioning of the device.

• Do not interrupt communication between the Chromeleon softwareand the device at any time during the procedure.

• At the beginning of the update process, a message appears showingthe firmware version currently installed in the device and theversion that will be transferred from the Chromeleon software. Ifthe firmware installed in the device is a later version than theversion in the Chromeleon software, cancel the download.



3. Monitor the Audit Trail of the Instrument Configuration Managerprogram to see whether the firmware update was successful orfailed.

¨ If the firmware update failed, turn the device off and on againand repeat the firmware update.

¨ If the firmware update fails repeatedly, contact Thermo FisherScientific Technical Support for assistance.

4. After a successful firmware update, requalification of the device maybe required. See the release notes for a recommendation.



7.11 Replacing the DoorsWhen

Damage of door

TIP The maintenance procedures do not require that you remove thedoors. If this should ever be required for a specific reason or procedure,follow the related steps in this section.

Parts required

Replacement door

Follow these steps

NOTICE

To avoid damage to the door hinges, be careful when performing thefollowing sequence of steps and do not apply force.

1. To remove a door, push the door upward while opening.Open the door to a position in which the two hinges on the housingare aligned in the grooves on the door. You can remove the dooronly when the hinges are in the grooves.

Figure 27: Unhinging a door

No. Description

1 Hinge on the housing

2 Reception groove on the door

2. Slightly tilt the door to the outside, away from the housing, andremove the door.



3. To install the door, align the door with the hinges on the housing. Becareful not to clamp tubing or capillaries between the door and theenclosure.

4. Insert the hinges in the groove, by pushing up and slightly turningthe door.

5. Push the door downward to lock it in place.You can close the door only when it is properly installed.



7.12 Transporting or Shipping the DeviceIf you want to transport the device to a new location or if you need toship the device, first prepare the device for transport and then move orship the device as required. Follow the instructions in this section.

Observe the following safety guidelines:






Follow these steps

1. Prepare the device for transport. See Preparing the Device forTransport ( page 128).

2. The step depends as follows:

¨ To transport the device to a new location, follow the instructionsin Transporting the Device to a New Location ( page 129).

¨ To ship the device, follow the instructions in Shipping the Device( page 130).

7.12.1 Preparing the Device for Transport

To prepare the device for transport, follow these steps:

1. Perform a long-term shut down of the device (see Long-TermShutdown ( page 95)).

2. Turn off the device with its main power switch and disconnect thepower cord.

3. Remove all cables and flow connections to other devices.

4. Remove the flow cell from the detector, and store the flow cell in itspackaging. Install the diagnostic cell to the flow cell opening of thedetector.



NOTICE

The flow cell opening on the device is sensitive to dust and debris.

If no flow cell is installed to the detector, install a diagnostic cell to theflow cell opening of the detector.

5. Remove the device or slide-in module from the system stack asrequired:Install the carrying handles and remove the device from theVanquish system. Follow the instructions on dismounting the systemstack in the Transporting or Shipping the System section of theVanquish System Operating Manual.

‑or‑

Remove the slide-in module from the device enclosure in the systemstack (see Removing the Slide-In Module ( page 131)).

See also



7.12.2 Transporting the Device to a New Location

Preparations

Prepare the device for transport. See Preparing the Device for Transport( page 128).

Follow these steps

1. Observe the notes for handling and lifting the device safely.

2. Transport the device to the new location.

3. Install and set up the device in the system stack. Follow theinstructions on mounting the system stack in the Vanquish SystemOperating Manual.

4. Set up the device:

a) Connect the device and set up flow connections (see Installation( page 45)).

b) Prepare the device for operation (see Preparing the Device forOperation ( page 82)).

5. Before starting an analysis, let the device equilibrate and be surethat it is ready for operation.



7.12.3 Shipping the Device

Preparations

Prepare the device for transport. See Preparing the Device for Transport( page 128).

Follow these steps

1. Follow the unpacking instructions in this manual in the reverseorder.Use only the original packing material and shipping container. If theoriginal shipping container is not available, appropriate containersand packing material can be ordered from the Thermo FisherScientific sales organization.

2. If you need to return the device to Thermo Fisher Scientific for depotrepair, contact your local Thermo Fisher Scientific supportorganization for the appropriate procedure.

CAUTION—Possible Contamination

Hazardous substances may have contaminated the device duringoperation and may cause personal injury to service personnel.

• Decontaminate all parts of the device that you want to return forrepair.

• Fill in and sign the Health and Safety Form. Thermo Fisher Scientificrefuses to accept devices for repair if the Health and Safety Form ismissing, incompletely filled in, or unsigned.

Restarting the Device after Shipping

To restart the device after shipping, follow these steps:

1. Follow the unpacking instruction in this operating manual.

2. Install and set up the device in the system stack. Follow theinstructions on mounting the system stack in the Vanquish SystemOperating Manual.

3. Set up the device:

a) Connect the device and set up flow connections (see Installation( page 45)).

b) Prepare the device for first-time operation (see Preparing theDevice for Operation ( page 82)).

4. Before starting an analysis, let the device equilibrate and be surethat it is ready for operation.



7.13 Replacing the Slide-In Module

7.13.1 Removing the Slide-In Module





Tools required

Screwdriver, Torx T20

Preparations

1. Prepare the device for transport. See Transporting or Shipping theDevice ( page 128).

Follow these steps

1. Loosen the four captive screws on the front left and front right ofthe device.

Figure 28: Captive screws on the slide-in module

2. Push all tubing and capillaries, which are present in the tubing chaseof the Vanquish system modules, into the tubing chase. Otherwise,you will not be able to remove the slide-in module properly from theenclosure in the next step.

3. Grasp the slide-in module by the leak tray, or by the lamp housecover and the partition panel, and pull the module out of theenclosure by approximately 10 cm.



Figure 29: Pulling out the slide-in module

NOTICE

The slide-in module can fall down when pulling it out of the enclosuretoo far.

Pull out the slide-in module just far enough so that you can grasp it onboth sides from below.

4. Remove the slide-in module from the enclosure. The following stepsrequire a team effort:

a) Take the slide-in module on both sides from below.

b) Pull the slide-in module from the rails towards the front.

c) Place the slide-in module on a clean and stable surface.

7.13.2 Returning the Slide-In Module

Preparation

If not yet done: Remove the slide-in module from the enclosure. SeeRemoving the Slide-In Module ( page 131).

Follow these steps

1. Install the slide-in module to the enclosure of the replacementdevice. Follow the steps for inserting the slide-in module in theenclosure and tightening the captive screws in Installing the Slide-InModule ( page 133).

2. Follow the instructions in Shipping the Device ( page 130).



NOTICE

Shipping the slide-in module improperly leads to damage to the device.

Always ship the slide-in module as described in this operating manual.

CAUTION—Possible Contamination

Hazardous substances may have contaminated the device duringoperation and may cause personal injury to service personnel.

• Decontaminate all parts of the device that you want to return forrepair.

• Fill in and sign the Health and Safety Form. Thermo Fisher Scientificrefuses to accept devices for repair if the Health and Safety Form ismissing, incompletely filled in, or unsigned.

7.13.3 Installing the Slide-In Module





Tools required

Screwdriver, Torx T20

Preparations

1. Unpack the replacement device. Remove the slide-in module fromits enclosure as described in Removing the Slide-In Module( page 131).

2. Verify that the device enclosure into which the slide-in module shallbe installed is clean. If required, clean the inner and outer surfacesof the enclosure. See Cleaning or Decontaminating the Device( page 104).

3. When installing the slide-in module to an enclosure in the systemstack, check that the enclosure is placed correctly in the stack.



Follow these steps

1. Push all tubing and capillaries, which are present in the tubing chaseof the Vanquish system modules, into the tubing chase. Otherwise,you will not be able to insert the slide-in module properly into theenclosure in the next step.

2. On the slide-in module, check that you can move the captive screwsback and forth with your fingers. If you cannot, screw them in untilyou can.

3. Insert the slide-in module in the enclosure. The following stepsrequire a team effort:

a) Take the slide-in module on both sides from below.

b) Lift the slide-in module to the height of the enclosure.

c) Place the slide-in module in the enclosure so that the modulesits in the enclosure by approximately 25 cm.

d) Push the slide-in module onto the rails and into the enclosureuntil the slide-in module sits completely in the enclosure.

Figure 30: Inserting the slide-in module

4. To facilitate tightening the slide-in module in the enclosure, pull outthe module a few millimeters (approximately 2 to 5 mm).

5. With the screwdriver, move each captive screw back and forth whilepressing inward until the screw slips into the thread.

6. Push the slide-in module back into the enclosure as far as it goes in.

7. Gradually and evenly, tighten the four captive screws on the slide-inmodule hand-tight.



NOTICE

• Verify that the screws are tightened. Pull the slide-in module by theleak tray towards the front and check whether the screws move. Ifthey do not move, the slide-in module is installed properly.

• If the screws move, tighten the screws further. With a torquewrench, the recommended torque is 1.2 Nm.

8. Set up and restart the device.

7.13.4 Setting Up the Slide-In Module

After you have reinstalled the slide-in module in the enclosure, set upand restart the device.

Follow these steps

1. Set up the slide-in module:

a) Connect the slide-in module and set up flow connections (seeInstallation ( page 45)).

b) Prepare the slide-in module for first-time operation (seePreparing the Device for Operation ( page 82)).

2. Prepare all other modules of the Vanquish system for operation andrestart them. Refer to the Operating Manuals for the modules.

3. Before starting an analysis, let the chromatography systemequilibrate and be sure that it is ready for operation.

4. In the Chromeleon software, run the device-specific PerformanceQualification (PQ) tests.

5. When the test is completed, update the QualificationDoneparameter.

8 • Troubleshooting


8 TroubleshootingThis chapter is a guide to troubleshooting issues that may arise duringoperation of the device.



8.1 General Information about TroubleshootingThe following features help you to identify and eliminate the source forproblems that may arise during operation of the device.

TIP For information about operating issues that might occur during theoperation of a Vanquish system, refer to the Vanquish System OperatingManual.

If you are unable to resolve a problem following the instructions givenhere or if you experience problems that are not covered in this section,contact Thermo Fisher Scientific Technical Support for assistance. Seethe contact information at the beginning of this manual.

To facilitate device identification, have the serial number and technicalname available when communicating with Thermo Fisher Scientific.

Status Indicators

The status indicator LED bar on the front side of the device and theSTATUS LED on the keypad inside provide quick visual feedback on theoperational status of the device. If the device firmware detects aproblem, the status indicators are red. The problem is reported to theChromeleon software and a message appears in the Audit Trail.

Alarms

Leaks are a potential safety issue. Therefore, if a leak sensor detectsleakage, beeping starts to alert you in addition to the message in theChromeleon Audit Trail and the status indicators changing to red. Followthe instructions in this manual to find and eliminate the source for theleakage.

Chromeleon Audit Trail Messages

If the device firmware detects a problem, the problem is reported to theChromeleon software.

The Chromeleon software logs information about all events related toinstrument operation for the current day in an Audit Trail. The AuditTrail is named with the current date, using the format yyyymmdd. Forexample, the Audit Trail for May 15, 2019, is named 20190515.

The Instrument Audit Trails can be found on the ePanel Set (AuditePanel). In addition, Audit Trails for each instrument are available in theConsole Data view, in the folder of the Instrument.



Messages in the Chromeleon Audit Trail are preceded by an icon. Theicon identifies the seriousness of the problem (refer to the ChromeleonHelp). For possible causes and remedial actions, see Messages( page 140).

Firmware Failure

If a firmware failure occurred during operation of the module, anexception log has been created about the processes during the firmwarefailure. The firmware sends the exception log to the Audit Trail when themodule is connected in the Chromeleon software.

In this case, observe the following:

• Send the Audit Trail as .cmbx file to the Technical Support beforeyou clear the log.

• To clear the exception log and continue operation of the module,perform the command ExceptionLogClear.

For more information, refer to the Chromeleon Help.



8.2 MessagesThe table lists the most frequently observed messages for the deviceand provides troubleshooting assistance.

Each message consists of a code number and a text. The code number isthe unique identifier for the problem while the wording may change.Note the following:

• To facilitate finding a message, the table lists the messages sorted bycode.

• If you cannot find the code you are looking for, check the messagetext. The two messages "Unexpected module behavior" and"Module malfunction detected" can be assigned to different codes.See the beginning of the table for more information.

TIP If you are unable to resolve the problem following the instructions inthis manual, or if you encounter a message not listed in the table, writedown the code and wording of the message and contact us. For details,see the Contacting Us section at the beginning of this manual.

Message and Code Description and Remedial Action

Unexpected module behavior. Code xx xx = Two-digit to four-digit code number.When the message appears, write down the message code andturn off the module. Wait for 5 seconds and turn on the moduleagain.

Module malfunction detected. Code xx xx = Two-digit to four-digit code number.When the message appears, write down the message code. Turnoff the module and contact Technical Support.

Code 33 Leak detected – eliminate withinapprox. xx seconds.

xx = the number of seconds within the leak must be resolvedFind and eliminate the source for the leakage (see ResolvingLiquid Leaks ( page 146)).

Code 34 Leak detected. Find and eliminate the source for the leakage (see ResolvingLiquid Leaks ( page 146)).

Code 36 Download failed. The firmware download has not been successful. Verify that thecorrect firmware file was selected. Repeat the download.

Code 37 Download firmware mismatch. The firmware download has not been successful. Verify that thecorrect firmware file was selected. Repeat the download.

Code 52 Module software incomplete.Download firmware (again).

The firmware is incomplete, for example, because thecommunication between the Chromeleon software and the devicewas interrupted during the firmware download. Repeat thedownload.

Code 89 Liquid leak sensor missing. Contact Thermo Fisher Scientific Technical Support for assistance.Note that the device can be operated nevertheless, even if theleak sensor is defective or not present, if leak detection isdisabled.




Code 90 Download firmware mismatch –invalid version.

You tried to download a firmware with an earlier version numberthan the firmware that is currently installed in the device.Downgrading the firmware may result in loss of functionality ormalfunctioning of the device. If required, repeat the downloadwith a firmware version later than the version currently installedin the device.

Code 7026 UV lamp malfunctionCode 7028 UV lamp malfunction

Turn on the lamp once more.Check the lamp connector.Check that the lamp cover is installed properly.If the message appears again, replace the UV lamp (see Replacingthe UV Lamp (Deuterium Lamp) ( page 107)).

Code 7029 VIS lamp malfunctionCode 7030 VIS lamp malfunction

Turn on the lamp once more.Check the lamp connector.Check that the lamp cover is installed properly.If the message appears again, replace the VIS lamp (see Replacingthe VIS Lamp (Tungsten Lamp) ( page 110)).

Code 7040 Data transfer error fromdetector to PC – check USB connection.

Check the USB connection. Use only the USB cables provided byThermo Fisher Scientific for the detector. Avoid CPU-intensive andtime-consuming operations on the PC during high-speed dataacquisition.

Code 7041 Command rejected – switchlamp on first.

Make sure that either the UV and/or VIS lamp are on. Operationcan be performed only if any of the lamps or both lamps are on.

Code 7044 Unexpected low lightintensity - check lamp(s) for correctinstallation.

Check the lamp installation.

Code 7045 Unexpected high lightintensity measured –check flow cellinstallation.

Check if the environmental conditions (ambient humidity andambient temperature) are within the specified operating range(see Physical Specifications ( page 153)).Check the flow cell (see Checking the Flow Cell ( page 145)).Run the detector over night (at least 12 hours, recommended24 hours) with an installed diagnostic flow cell and with the UVlamp (and VIS lamp, if available) turned on.If the message still appears, install a different flow cell or adiagnostic cell. Turn on any lamp (UV or VIS) and perform an auto-zero.

Code 7046 xx over-temperature -emergency standby in yy seconds.Code 7047 xx over-temperature -emergency standby after acquisitioncompleted (force shutdown in yy s).Code 7086 xx over-temperature –emergency standby entered now.

With xx = system or lamp house, yy = the number of secondsbefore emergency standby or shutdown is enteredAn over-temperature condition was detected.Turn off the detector and let the detector cool down.Check that the detector is operated within the specifiedenvironmental conditions (ambient temperature), see PhysicalSpecifications ( page 153).Check the cooling air intake and the ventilation holes on the rearside of the detector for dust. If dust is present, aspirate the dustand loose particles with a vacuum cleaner.




Code 7049 Wavelength calibration failed– check and flush flow cell.

Check the flow cell (see Checking the Flow Cell ( page 145)). Ifthe message still appears, proceed as follows:Check the lamps: If a lamp was newly installed, ensure that it isinstalled properly. If a lamp is old (counting, for example, morethan 2000 operating hours), replace the lamp (see Lamps( page 107)).

Code 7051 Bad flow cell position. The flow cell is not correctly positioned inside the flow cellopening.Check the flow cell installation (see Checking the Flow Cell( page 145)).Check the flow cell contact pins for proper alignment inside theflow cell opening.

Code 7056 Holmium validation failed –wavelength outside limits.

Repeat the validation (see Performing a Wavelength Validationand Calibration ( page 119)). If the message still appears,proceed as follows:Perform a calibration and repeat the validation again.Remove and install the flow cell. Flush the flow cell with pureHPLC-grade water with a flow rate of > 1 mL/min for severalminutes (Nano flow cells: Flush with pure HPLC-grade water withthe maximum possible flow rate for the respective range forseveral minutes).Install a different flow cell or a diagnostic cell (see Installing a FlowCell ( page 59) or Installing a Diagnostic Cell ( page 118)).Perform a validation. Perform a calibration and repeat thevalidation again. If the message disappears with a different cell,the flow cell that was previously installed may be clogged.If the error message persists, contact Technical Support.

Code 7057 Holmium validation failed –specified wavelength not found.

Repeat the validation. If the message still appears, proceed asfollows:Perform a calibration and repeat the validation again.Remove and install the flow cell. Flush the flow cell with pureHPLC-grade water with a flow rate of > 1 mL/min for severalminutes (Nano flow cells: Flush with pure HPLC-grade water withthe maximum possible flow rate for the respective range forseveral minutes).Check the lamp: If the lamp was newly installed, ensure that it isinstalled properly. If the lamp is old (counting, for example, morethan 2000 operating hours), replace the lamp. See Lamps( page 107).Install a different flow cell (see Installing a Flow Cell ( page 59)).Perform a validation. Perform a calibration and repeat thevalidation again. If the message disappears with a different cell,the flow cell that was previously installed may be clogged.

Code 7059 Calibration failed – valuesoutside specs.

Remove and install the flow cell.Flush the flow cell with pure HPLC-grade water with a flow rate of>1 mL/min for several minutes (Nano flow cells: Flush with pureHPLC-grade water with the maximum possible flow rate for therespective range for several minutes).If the error message persists, install a different flow cell or adiagnostic cell (see Installing a Flow Cell ( page 59)).




Code 7066 Invalid data rate/responsetime combination. Filter not effective.

Select a higher data rate or longer response time for the responsetime filter to be effective. Use the Chromeleon InstrumentMethod Wizard for valid combinations.

Code 7111 Lamp cover not in place -check cover position.

Check that the lamp cover is installed properly. For correctinstallation of the lamp house cover, see Lamps ( page 107).

Code 7124 Unexpected low lightintensity - check flow cell for correctinstallation.

There is no light on measurement diode.Check the flow cell installation (see Checking the Flow Cell( page 145)).Flush the flow cell with pure HPLC-grade water for severalminutes (flow > 1 mL/min., Nano flow cells: Flush with pure HPLC-grade water with the maximum possible flow rate for therespective range for several minutes).If the error message persists, replace the flow cell.

Code 7125 Reference/measurementchannel calibration mismatch.

There is a significant deviation between reference channel andmeasurement channel calibration values.Check the flow cell installation (see Checking the Flow Cell( page 145)).Check that the lamp is properly installed.Ensure that the eluent does not show excessive absorption duringcalibration.If the error message persists, replace the flow cell.

Code 7130 Command rejected - baselineadjustment in progress.

An auto-zero append process is collecting data.• Wait until the auto-zero append process is completed.• Repeat the auto-zero command.

Code 7133 Acquisition aborted – datarate limit exceeded.

The data collection rate is too high and exceeds the limits for theselected wavelength configuration. In the Chromeleon software,select a lower data collection rate or reduce the number ofwavelengths selected.

Code 7136 Invalid measurement channelzero order position - check flow cell andlamp installation.

Remove and install the flow cell.Flush the flow cell with pure HPLC-grade water with a flow rate of>1 mL/min for several minutes (Nano flow cells: Flush with pureHPLC-grade water with the maximum possible flow rate for therespective range for several minutes).

Code 7139 Reference scan speedmismatch.

The baseline (reference) scan speed and the absorption scanspeed do not match. The absorption calculation may be affected.Repeat the baseline scan with the same scan speed that is usedfor the scan.

Code 7140 No or unknown xx installed. With xx = flow cell, lamp, or opticsThe flow cell or lamp is not installed or does not have an ID chip.Install a flow cell (see Installing a Flow Cell ( page 59)) or install alamp (see Lamps ( page 107)).Use lamp(s) or a flow cell with ID chip.If the optics is affected, contact Technical Support.

Code 7141 Acquisition aborted (flow cellremoved).

The flow cell has been removed while an acquisition is running.• Re-install a flow cell (see Installing a Flow Cell ( page 59)).• Restart acquisition.




Code 7143 Internal process failed – noflow cell.

The flow cell has been removed while an internal process wasrunning.Re-install a flow cell (see Installing a Flow Cell ( page 59)). It maybe necessary to repeat the previous command or operation.



8.3 Checking the Flow CellWhen

Related messages that require a flow cell check appear in the Audit Trail.

Follow these steps

1. Check that the flow cell is installed properly.

2. Remove and re-install the flow cell.

3. Flush the flow cell with pure HPLC-grade water with a flow rate of>1 mL/min for several minutes (Nano flow cells: Flush with pureHPLC-grade water with the maximum possible flow rate for therespective range for several minutes).

4. Install a different flow cell and repeat the action during which themessage appeared with the flow cell that was previously installed.

Result Steps

If the message disappears with adifferent flow cell

The problems are caused by the flow cellthat was previously installed. If yoususpect a contamination or an elevatedbackpressure, perform a flushingprocedure with the flow cell that waspreviously installed (see Preparing theFlow Cell for Storage).

If the message still appears The problem is caused by the flow pathor components in the flow path beforethe detector or by the detector.Install a diagnostic cell and repeat theaction during which the messageappeared with the flow cell that waspreviously installed.• If the message disappears with the

diagnostic cell: The problems arecaused by the flow path or componentsin the flow path before the detector.

• If the message still appears: Theproblems are caused by the detector.

See also

2 Messages ( page 140)



8.4 Resolving Liquid LeaksWhen

The leak sensor is wet. The leak sensor reports leakage.

Parts and additional items required

• Replacement part as required

• Cloth or tissue

Preparations

When resolving leakage, observe the safety guidelines and general rulesfor maintenance and service as presented in Maintenance and Service( page 99).

Follow these steps

1. Locate the source of the leak. Leakage usually occurs at aconnection. However, leakage may also have occurred inside theflow cell. In this case, leakage may occur at the drain outlet of theflow cell (see figure).

Figure 31: Flow cell drain outlet



Follow the steps depending on the location of the leak:

Location of the leak Steps

Flow cell inlet and/or outlet 1. Tighten the connection where liquid isvisible.

2. If the connection seems tight but is stillleaking, remove the connection/fittingand check for damage.

3. If necessary, replace the inlet capillaryor waste line.

Drain outlet of the flow cell (see figure),or connection from flow cell toconnection unit

1. Remove the flow cell from the detectorand inspect the flow cell for signs ofleakage.

2. If signs of leakage are present at theflow cell, replace the flow cell.

3. With a cloth or tissue, thoroughly absorb all liquid that has collectedin the leak tray and under the leak sensor. Be careful not to bend thesensor.

4. Allow the sensor to adjust to the ambient temperature for a fewminutes.

5. If leakage is no longer reported, you can resume operation.

See also




9 • Specifications


9 SpecificationsThis chapter provides the physical and performance specifications,including information about the materials used in the flow path of thedevice.



9.1 Performance Specifications

9.1.1 Detector

The device performance is specified as follows:

Type Specification

Optical design UV/VIS/NIR photometer (tunable Czerny-Turner monochromator) withadditional internal reference beam

Light source • Deuterium lamp for UV and VIS• Tungsten lamp for VIS and NIR (optional for VC-D40)• Lamp house temperature control for stable operation

Wavelength range VC-D40: 190 to 750 nmVF-D40: 190 to 900 nmThe tungsten lamp is recommended for wavelengths > 345 nm.

Spectral bandwidth typ. 6 nm at 254 nm

Wavelength Accuracy ± 1 nm

Wavelength Repeatability ± 0.1 nm

Wavelength Calibration Internal calibration with zero order of monochromator spectrum

Wavelength Validation Internal validation with holmium-oxide filter

Signal channels VC-D40: Up to 2 signal channelsVF-D40: Up to 4 signal channels

Reference channel Reference beam with same wavelength as measurement beam

Data collection rates Data collection rates under Chromeleon 7 data system. Other maximumdata rates may be applicable under different data systems.Single-channel:VC-D40: Adjustable, 0.2 Hz– 125 HzVF-D40: Adjustable, 0.2 Hz– 250 HzMulti-channelAdjustable, up to 5 Hz, depending on the number of wavelengths and thedistance between the wavelengths (in nm)

Filter response times (inseconds)

0s (no filter) to 20s

Spectra scans 5 scan speeds up to max. 100 nm/s

Noise (Single-channel mode) < ± 2.5 µAU, wavelength 254 nm, only deuterium lamp on< ± 5.0 µAU, wavelength 520 nm, only tungsten lamp turned onStandard flow cell with 1 mL/min HPLC-grade water, degassed, column inflow path, time constant: 2 seconds, data collection rate: 1 HzAll values reached within 60 minutes of warm-up (typical).



Type Specification

Noise (Multi-channel mode) <± 10.0 µAU (typical: <± 7.0 µAU), wavelengths: 254 nm and 280 nm, onlydeuterium lamp turned on<± 10.0 µAU (typical: <± 7.0 µAU), wavelengths: 450 nm and 520 nm, onlytungsten lamp turned onStandard flow cell with 1 mL/min HPLC-grade water, degassed, column inflow path, time constant: 2 seconds, data collection rate: 1 HzAll values reached within 60 minutes of warm-up (typical).

Drift < 0.1 mAU/hour, wavelength: 254 nm, only deuterium lamp turned on< 0.1 mAU/hour, wavelength: 520 nm, only tungsten lamp turned onStandard flow cell with 1 mL/min HPLC-grade water, degassed, column inflow path, time constant: 2 secondsAll values reached within 60 minutes of warm-up (typical).

Linearity < 5% at 2.5 AU (ASTM), wavelength: 272 nm, component: caffeine, onlydeuterium lamp turned onStandard flow cell

USB communication 1 USB port (USB 2.0, "B" type connector)1 USB hub with 3 ports (USB 2.0, "A" type connectors)

I/O Interface 2 digital I/O ports

System Interlink 2 System Interlink ports (RJ45-8 connectors)

Analog output 2 analog outputs via optional plug-in expansion board to output absorptionchannelsResolution: 20 bitMaximum data rate: 50 HzOutputs can be configured via software (output voltage range 0 to 1 V or0 to 10 V, sensitivity and offset)

Control Chromeleon 7The device can be operated also with other data systems. For details,contact the Thermo Fisher Scientific sales organization.Keypad with 3 buttons for performing certain functions directly from thedevice

Materials in the flow path See the Specifications for the flow cells.NOTICE For information about the chemical resistance of materials refer tothe technical literature.

Safety features Power-up check of optics, cooling fans, motors and electronicsLeak detection and safe leak handling

Good Laboratory Practice (GLP)features

Predictive Performance functions for scheduling maintenance proceduresbased on the actual operating and usage conditions of the device. Thisincludes monitoring of lamp age, and lamp ignitions for UV and VIS lamp.All system parameters are logged in the Chromeleon Audit Trail.

Temperature reference conditions for noise, drift and linearity specifications

For drift tests, ASTM requires that temperature changes stay below 2 °C/hour over a period of one hour. The drift specification above is based onthese conditions. Larger changes in the ambient temperature will resultin a larger drift.



For best performance, minimize the frequency and the amplitude ofchanges in the ambient temperature to 1 °C/hour.

Time constant and response time

According to ASTM E1657-98, the time constant is converted to theresponse time by multiplying by the factor 2.2 (i.e., response time =2.2 x time constant).

9.1.2 Flow Cells

The flow cell specifications are as follows:

Type Pathlength

Illuminated volume

Pressurelimit

Materials in theflow path

Biocompatibility

Standard flow cell, SST 10 mm 11 µL 12 MPa Stainless steel,fused silica, PEEK,fluoropolymers,titanium

No

Standard Bio flow cell,PEEK

10 mm 11 µL 5 MPa PEEK, Fused silica Yes

Semi-micro flow cell, SST 7 mm 2.5 µL 12 MPa Stainless steel,fused silica, PEEK,fluoropolymers,titanium

No

Semi-micro Bio flow cell,PEEK

7 mm 2.5 µL 5 MPa PEEK, fused silica Yes

Semi preparative flow cell 0.4 mm 0.7 μL 10 MPa PEEK, fused silica Yes

Nano flow cell 10 mm* 3 nL 20 MPa Fused silica Yes

Capillary flow cell 10 mm* 45 nL 20 MPa Fused silica Yes

Mic flow cell 10 mm* 180 nL 20 MPa Fused silica Yes

* There is no exact definition of the light path in capillary flow cells as is for conventionalflow cells. Therefore, you should not use this value for calculating the absorptioncoefficient or the detector sensitivity according to the Lambert-Beer law.



9.2 Physical SpecificationsThe physical conditions of the device are specified as follows:

Type Specification

Range of use Indoor use only

Ambient operatingtemperature

5 °C - 35 °C

Ambient storage temperature -20 °C - 45 °C

Ambient operating humidity 20% - 80% relative humidity (non-condensing)

Ambient storage humidity Maximum 60% relative humidity (non-condensing)

Operating altitude Maximum 2000 m above sea level

Pollution degree 2

Power requirements Wide range, 100 – 240 V AC, ± 10 %; 50/60 Hz; max. 245 W / 255 VA

Overvoltage category II

Emission sound pressure level typically < 61 dB(A)

Dimensions(height x width x depth)

15.9 x 42 x 62 cm

Weight 16 kg (with diagnostic cell installed)

10 • Accessories, Consumables and Replacement Parts


10 Accessories,Consumables andReplacement PartsThis chapter describes the standard accessories that are shipped withthe device and the accessories that are available as an option. Thischapter also provides information for reordering consumables andreplacement parts.



10.1 General InformationThe device must be operated only with the replacement parts andadditional components, options, and peripherals specifically authorizedand qualified by Thermo Fisher Scientific.

Accessories, consumables, and replacement parts are always maintainedat the latest technical standard. Therefore, part numbers are subject tochange. If not otherwise stated, updated parts will be compatible withthe parts they replace.



10.2 Ship KitThe ship kit includes the items listed in the table. The kit content issubject to change and may vary from the information in this manual. Seethe content list included in the kit for the most recent information aboutthe kit content at the time when the device is shipped.

Ship kit

Item Quantity inshipment

Partition panel plug for guiding insulated capillaries 1

System interlink cable 1

Tubing bracket 1

USB cable, USB 2.0 high-speed, type A to type B 1

Viper capillary, column compartment – variable wavelengthdetector

1

Waste line 1

For reordering information, see Consumables and Replacement Parts( page 159).



10.3 Optional AccessoriesFlow cells and Flow cell accessories

Item Part No.

Standard flow cell, SST, 10 mm 6077.0250

Semi-micro flow cell, SST, 7 mm 6077.0360

Standard Bio flow cell, PEEK, 10 mm 6077.0200

Semi-micro Bio flow cell, PEEK, 7 mm 6077.0300

Nano flow cell, fused silica, 10 mm* 6074.0270

Capillary flow cell, fused silica, 10 mm* 6074.0280

Micro flow cell, fused silica, 10 mm* 6074.0290

Semi-preparative flow cell, PEEK, 0.4 mm 6074.0320

Diagnostic cell 6077.0190

Flushing and injection kit for flow cells, including syringe 6078.4200

Viper capillary, 0.13 x 450 mm, stainless steelFor connecting column and flow cell inlet when usingUltiMate 3000 flow cells

6040.2345

* There is no exact definition of the light path in capillary flow cells as is for conventionalflow cells. Therefore, you should not use this value for calculating the absorptioncoefficient or the detector sensitivity according to the Lambert-Beer law.

TIP UltiMate 3000 variable wavelength detector flow cells have theconnection unit on the left side. To connect the column outlet to theconnection unit installed on the left side of the flow cell in a Vanquishvariable wavelength detector, Thermo Fisher Scientific recommendsusing the capillary of 450 mm length listed in the table above.

Miscellaneous

Item Part No.

VIS lamp (tungsten lamp) 6074.2000

DAC boardProvides two analog outputs. Contact Thermo Fisher ScientificTechnical Support for installation.

6083.0900



10.4 Consumables and Replacement PartsCapillaries and tubing

Description Part No.

Waste line 6036.2425

For system capillaries, refer to the Vanquish System Operating Manual.

Lamps


Standard UV lamp (deuterium lamp) 6077.1110

High sensitivity UV lamp (deuterium lamp) 6077.1111

VIS lamp (tungsten lamp) 6074.2000

Miscellaneous


Front door kit, including right door and left door 6083.3018

Packing material for detector 6083.0090

Fuses kit, Vanquish systemThe kit includes the appropriate fuses for the Vanquish systemmodules. For the device, use only 3.15AT, 250V AC, slow-blowfuses.

6036.0002

Interface cables


Digital I/O signaling cable, 6-pin, cable length: 5 m 6036.0006

USB cable, type A to type B, high-speed, USB 2.0Cable length: 1 m

6035.9035

USB cable, type A to type B, high-speed, USB 2.0Cable length: 5 m

6911.0002

Power cords


Power cord, Australia 6000.1060

Power cord, China 6000.1080

Power cord, Denmark 6000.1070

Power cord, EU 6000.1000

Power cord, India, SA 6000.1090

Power cord, Italy 6000.1040




Power cord, Japan 6000.1050

Power cord, UK 6000.1020

Power cord, USA 6000.1001

Power cord, Switzerland 6000.1030

11 • Appendix


11 AppendixThis chapter provides additional information about compliance,UV cutoff wavelengths, and the use of the digital I/O ports.

11 • Appendix


11.1 Compliance Information

11.1.1 Declarations of Conformity

CE Declaration of Conformity

The device has satisfied the requirements for the CE mark and iscompliant with the applicable requirements.

cTUVus Compliance

The cTUVus label on the device indicates that the device has satisfiedthe requirements for the cTUVus mark. Compliance with the applicablestandards has been evaluated by TÜV Rheinland of North America Inc.

RoHS Compliance

This product complies with the RoHS (Restrictions of HazardousSubstances) directives:

• European RoHS DirectiveDirective on the restriction of the use of certain hazardoussubstances in electrical and electronic equipment

The CE mark on the device indicates that the product is compliantwith the directive.

• China RoHS regulationsMeasures for Administration of the Pollution Control of ElectronicInformation Products

One of the following logos may be present on the device ifapplicable:

Logo Description

The green logo marks items that do not contain the hazardoussubstances identified by the regulations.

The orange logo including a one-digit or two-digit number marksitems that contain hazardous substances identified by theregulations. The number indicates the environment-friendly useperiod (EFUP) of the item. During this period, the item (when used asintended) will not cause serious damage to human health orenvironment.For more information, go to http://www.thermofisher.com/us/en/home/technical-resources/rohs-certificates.html

http://www.thermofisher.com/us/en/home/technical-resources/rohs-certificates.html

http://www.thermofisher.com/us/en/home/technical-resources/rohs-certificates.html

11 • Appendix


11.1.2 WEEE Compliance

This product is required to comply with the European Union’s WasteElectrical & Electronic Equipment (WEEE) Directive. It is marked with thefollowing symbol:

Figure 32: WEEE symbol

Thermo Fisher Scientific has contracted with one or more recycling ordisposal companies in each European Union (EU) Member State, andthese companies should dispose of or recycle this product. For furtherinformation, contact Thermo Fisher Scientific.

11.1.3 FCC Compliance

This equipment has been tested and found to comply with the limits fora Class A digital device, pursuant to part 15 of the U.S. FCC Rules.

These limits are designed to provide reasonable protection againstharmful interference when the equipment is operated in a commercialenvironment. This equipment generates, uses, and can radiate radiofrequency energy and, if not installed and used in accordance with theinstructions, may cause harmful interference to radio communications.Operation of this equipment in a residential area is likely to causeharmful interference, in which case the user will be required to correctthe interference at his expense.

11.1.4 NIST Compliance

The holmium-oxide glass filter that is used in the device meets therequirements of the National Institute of Standards and Technology(NIST). For further information, refer to the Declaration of Conformityfor the Holmium-Oxide Glass Filter.

11.1.5 Manual Release History

Revision Covering

2.0 VC-D40, VF-D40

1.1a VF-D40

1.1 VF-D40

11 • Appendix


The instructions were prepared in English (original instructions). Otherlanguage versions are translations based on the English originalinstructions.

11 • Appendix


11.2 UV Cutoff Wavelengths of SolventsThe UV cutoff wavelength is the minimum effective wavelength for themeasurement. The mobile phase composition affects its UV cutoffwavelength.

In general, mobile phases are solvents, such as, water, acetonitrile,methanol, or other substances. They may also contain salts, such assodium hydroxide (NaOH).

Most solvents have a UV cutoff wavelength within the spectral range ofthe device. For optimum measurement results, perform quantitativemeasurements at a wavelength that is sufficiently above the UV cutoffwavelength. The UV cutoff wavelength also depends on the quality ofthe solvent. For information about the UV cutoff wavelength for asolvent, contact the manufacturer of the solvent.

The refractive index of different solvents can differ. Therefore, changingthe mobile phase composition can lead to baseline fluctuations.

11 • Appendix


11.3 Digital I/OThe digital I/O ports (Dig I/O) can be used to exchange digital signalswith external devices. Each port provides:

• one digital input

• one relay output

Pin Assignment

Figure 33: Digital I/O port

Pin Description

1 Not used

2 Relay output — Relay_NC (Normally Closed contact)

3 Ground — GND

4 Digital input — Input

5 Relay output — Relay_COMCOM is the common contact for NO and NC. If the relay is not activated or ifthe device is turned off, the connection is between COM and NC. If the relayis activated, the connection is between COM and NO.

6 Relay output — Relay_NO (Normally Open contact)

The next table lists the functions assigned to the connector pins and thecolor of the cable wire connected to each pin.

Pin Wire Color Signal Name Signal Level Remarks

1 Pink Not used

2 Gray Relay output— Relay_NC

Potential free0-24 V, 0-100 mA

Opening contact

3 Green Ground —GND

Ground Reference potential

11 • Appendix


Pin Wire Color Signal Name Signal Level Remarks

4 Yellow Digital input —Input

Input (low active):On: 0-0.4 VOff: 2.2-5 VPull-up resistor: 47 kΩ to 5 V

Digital input; reference potential isground. Note the following:• The maximum input voltage at the

input must not exceed +5 V withreference to ground.

• The minimum input voltage mustnot be lower than the groundpotential.

5 White Relay output— Relay_COM

Potential free Common contact for NO and NC

6 Brown Relay output— Relay_NO

Potential free0-24 V, 0-100 mA

Closing contact

Prerequisites

To use the digital I/O functionality, the following prerequisites must befulfilled:

• The digital I/O port must be connected to the external device withthe digital I/O signaling cable (part no. 6036.0006).

• The inputs and outputs that you want to use must be selected in theInstrument Configuration Manager.

Connecting a Digital I/O Port

1. Plug the 6-pin connector of the cable into the digital I/O port thatyou want to use.

2. For each relay output or digital input to be used, connect theappropriate signal wire and ground wire to the correspondingconnectors on the external device. For details, refer to thedocumentation provided with the external device.

Selecting the inputs and outputs in the Chromeleon software

1. In the dialog box for the device, on the Inputs and Outputs pages,select the inputs and outputs that you want to use. The numberingin the dialog box corresponds to the numbers on the port.

11 • Appendix


Index


IndexA

accessories ............................................... 44, 155optional ..................................................... 158ship kit ....................................................... 157

additives ........................................................... 27information ................................................. 27

audit trail ........................................................ 138Audit Trail messages ...................................... 140Autozero........................................................... 93

Bbaseline behavior ............................................. 93buffers .............................................................. 27

concentration.............................................. 27information ................................................. 27

Ccapillaries

guide ........................................................... 63install ........................................................... 67Viper............................................................ 67

CE mark .......................................................... 162chloride concentration ..................................... 28Chromeleon...................................................... 40

audit trail................................................... 138device setup ................................................ 74module setup .............................................. 74Predictive Performance............................. 106set wavelengths .......................................... 91Smart Shutdown.......................................... 94Smart Standby............................................. 94Smart Startup .............................................. 83

cleaning .......................................................... 104condensation.............................................. 50, 55connectors........................................................ 53consumables .......................................... 155, 159cTUVus mark .................................................. 162

Ddata collection rate .............................. 88, 91, 92

guidelines .................................................... 92decontamination.................................... 104, 105delivery............................................................. 44

detector waste line .......................................... 71deuterium lamp (UV) ............................... 38, 107device

optimization ................................................ 90preparing for operation .............................. 82shutdown .................................................... 94

diagnostic cell................................................... 59Dig I/O ...................................................... 54, 166digital I/O.................................................. 54, 166disk space ......................................................... 91door.................................................................. 51

open ............................................................ 51remove ...................................................... 100replace....................................................... 126unhinge ............................................. 100, 126

drain system..................................................... 72drainage ........................................................... 72

Eequilibration..................................................... 83ExceptionLogClear (Chromeleon)................... 139

FFCC ................................................................. 163features overview ............................................ 32firmware failure.............................................. 139firmware update ............................................ 124flow cell ............................................................ 36

check ......................................................... 145clean.......................................................... 115connect inlet ............................................... 70connect outlet ............................................. 71diagnostic cell............................................ 118exchange ................................................... 116flow connections ......................................... 68guidelines .................................................... 84handling .................................................... 115identification chip ....................................... 36installation................................................... 59no connection unit ...................................... 60remove ...................................................... 116shipment ................................................... 114storage ...................................................... 114types............................................................ 37

Index


waste line replacement............................. 121with connection unit ................................... 60

fuses ............................................................... 122

Ggloves ............................................................... 23guide hole......................................................... 63guidelines

flow cell handling ........................................ 84flow cell maintenance ............................... 115installation................................................... 46maintenance ............................................. 101operation..................................................... 77

Hholmium-oxide glass filter.............................. 163

Iidentification chip ............................................ 36

flow cell ....................................................... 36lamp ............................................................ 38

inlet capillary .................................................... 70install slide-in module .................................... 133installation........................................................ 45

capillaries and tubing .................................. 63safety guidelines ......................................... 46site requirements........................................ 49system ......................................................... 47

intended use..................................................... 20interior view ..................................................... 35Interlink ............................................................ 54

Kkeypad.............................................................. 78

UV................................................................ 79VIS ............................................................... 79

Llamp ................................................... 38, 89, 107

turn on ........................................................ 85UV lamp............................................... 38, 107VIS lamp .............................................. 38, 107

lamp replacement .......................................... 107deuterium lamp (UV) ................................ 107tungsten lamp (VIS)................................... 110

leak detection............................................. 39, 88leak sensor ..................................................... 146leak sensor mode ............................................. 88leakage ........................................................... 146LED bar ............................................... 78, 79, 138linearity ............................................................ 91liquid leaks...................................................... 146long-term shutdown ........................................ 95

Mmain power fuses ........................................... 122maintenance ............................................ 99, 104

cleaning ..................................................... 104decontaminating ....................................... 104doors ......................................................... 126firmware update ....................................... 124flow cells ................................................... 114fuses .......................................................... 122general rules.............................................. 103interval ...................................................... 104introduction .............................................. 100lamps......................................................... 107predictive performance............................. 106safety guidelines ....................................... 101waste line .................................................. 121

maintenance and servicecalibration ................................................. 119validation................................................... 119

messages ........................................................ 140move ................................................................ 48multi-channel mode ......................................... 86Mute Alarm ...................................................... 79

NNIST compliance............................................. 163

Ooperation.................................................... 40, 75

control elements ......................................... 78flow cells ..................................................... 84interrupt...................................................... 94power on/off ............................................... 81safety guidelines ......................................... 77

operational modemulti-channel mode.................................... 86single-channel mode ................................... 86

Index


optimization ..................................................... 90baseline behavior ........................................ 93data collection rate ..................................... 92parameters overview .................................. 91peak width................................................... 92response time ............................................. 92wavelength.................................................. 91

outlet waste line............................................... 71overview (functional) ....................................... 31

Ppeak resolution ................................................ 91peak width............................................ 88, 91, 92

optimization ................................................ 92pH range........................................................... 28power considerations................................. 49, 50power cord ....................................................... 49power on/off .................................................... 81power up .......................................................... 73Predictive Performance.................................. 106preparation

remove module......................................... 131protective clothing ........................................... 22

Rrating plate ....................................................... 19regulatory compliance ..................................... 30remove slide-in module ................................. 131reordering ...................................................... 155replacement parts .................................. 155, 159response time....................................... 88, 91, 92

optimization ................................................ 92RoHS mark...................................................... 162

Ssafety class ....................................................... 21safety glasses.................................................... 22safety guidelines

case of emergency ...................................... 26electrical safety ........................................... 23general ........................................................ 21general hazards ........................................... 23installation................................................... 46maintenance ............................................. 101operation..................................................... 77power cord.................................................. 49protective equipment ................................. 22

qualification of personnel ........................... 21servicing .................................................... 101

safety symbols............................................ 18, 19sensitivity ......................................................... 91service .............................................................. 99setup

hardware..................................................... 52software ...................................................... 74system arrangement ................................... 52

ship................................................................. 128ship kit ...................................................... 44, 157short-term shutdown ....................................... 94shutdown ......................................................... 94

long-term .................................................... 95short-term................................................... 94

signal channel................................................... 89signal words ..................................................... 18single-channel mode ........................................ 86site requirements ............................................. 49

condensation......................................... 50, 55power considerations............................ 49, 50power cord.................................................. 49

slide-in module............................................... 131install ......................................................... 133remove ...................................................... 131return ........................................................ 132

Smart Shutdown............................................... 94Smart Standby .................................................. 94Smart Startup ................................................... 83solvent

chloride concentration................................ 28information ................................................. 27pH range...................................................... 28

specifications.................................................. 149performance (detector) ............................ 150performance (flow cells) ........................... 152physical...................................................... 153

startup.............................................................. 83status indicator

LED bar .......................................... 78, 79, 138Status LED ..................................... 78, 79, 138

Status LED........................................... 78, 79, 138system arrangement ........................................ 52system equilibration......................................... 83System Interlink................................................ 54

Index


Ttransport ........................................................ 128troubleshooting.............................................. 137

general information .................................. 138messages................................................... 140

tubing ............................................................... 67tubing bracket .................................................. 63tubing chase ..................................................... 63tubing guides.................................................... 63tungsten lamp (VIS) .................................. 38, 107type label.......................................................... 19

UUniversal Serial Bus .......................................... 54unpack.............................................................. 42USB ................................................................... 54UV button......................................................... 79UV cutoff wavelengths ................................... 165UV lamp.............................................. 38, 89, 107

turn on ........................................................ 85UV_VIS.............................................................. 89

VViper fitting system .......................................... 67VIS button......................................................... 79VIS lamp ............................................. 38, 89, 107

turn on ........................................................ 85

Wwaste line ......................................................... 71Wavelength ................................................ 89, 91wavelength calibration............................. 89, 119wavelength switching....................................... 91wavelength validation .............................. 89, 119WEEE .............................................................. 163

Index


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