vdl annual report - iowa state university · 2015-12-21 · 2010 annual report . 2 isu veterinary...

2010 Annual Report

2 ISU Veterinary Diagnostic Laboratory

Table of Contents

ISU VDL Mission Statement ...................................................................................................................... 3

VDPAM Organizational Chart .................................................................................................................... 4

VDPAM External Advisory Board .............................................................................................................. 5

VDL Faculty List ........................................................................................................................................ 6

New VDL Faculty in 2012 .......................................................................................................................... 6

VDL Staff List ............................................................................................................................................ 7

Honors and Awards ................................................................................................................................... 9

New Tests Introduced in 2012 ................................................................................................................. 10

CYCADS - New VDL Section Formed ..................................................................................................... 11

2012 Statistical Data ............................................................................................................................... 12

Accessions ......................................................................................................................................... 12

Tests ................................................................................................................................................... 13

Submission Trends ............................................................................................................................. 13

Species Submission Data ................................................................................................................... 14

Surveillance Testing............................................................................................................................ 16

Bacteriology Testing ........................................................................................................................... 17

Antimicrobial Susceptibility Profiles ............................................................................................ 21

CYCADS Testing ................................................................................................................................ 31

Molecular and Health Assurance (HATS) Testing ............................................................................... 31

Pathology Testing ............................................................................................................................... 34

Serology Testing ................................................................................................................................. 34

Toxicology/Nutrition Testing ................................................................................................................ 37

Virology Testing .................................................................................................................................. 42

Rabies ........................................................................................................................................ 44

Glossary ................................................................................................................................................. 46

The Iowa State University Veterinary Diagnostic Laboratory (VDL)

is accredited as a full service laboratory by the American Association of Veterinary Laboratory Diagnosticians (AAVLD)

"Iowa State University does not discriminate on the basis of race, color, age, religion, national origin, sexual orientation, gender identity, genetic information, sex, marital status, disability, or status as a U.S. veteran. Inquiries can be directed to the Director of Equal Opportunity and Compliance, 3280 Beardshear Hall, (515) 294-7612."


ISU VDL Mission Statement

It is the mission of the Iowa State University Veterinary Diagnostic Laboratory to provide comprehensive and cutting edge diagnostic services to veterinarians, producers, and animal owners in Iowa and nationally. The lab is responsible for delivering accessible, timely, accurate, valid, and consistent test results to aid in the protection of animal and human health. Other services include a wide range of surveillance testing for early detection and identification of foreign animal and emerging domestic disease agents, as well as acts of bioterrorism directed at human and livestock populations and agricultural food supplies. The VDL also provides educational opportunities to professional and graduate students, as well as local, national and international scientists, diagnosticians and practitioners. Research is an important component of the tripartite mission as faculty and staff develop state-of-the-art diagnostic tools and techniques and also direct studies which provide new insights and deeper understanding of pathogenesis, transmission, and immunomodulation of infectious diseases.

Our Tripartite Mission

• Provide comprehensive and cutting edge diagnostic services • Deliver accessible, timely, accurate, valid and consistent test results • Provide educational opportunities to professional and graduate students, scientists, diagnosticians

and practitioners


VDPAM Organizational Chart


VDPAM External Advisory Board

Name Title/Position Company/Clinic City

Dr. Pat Halbur Chair of VDPAM, Executive Director of the ISU VDL, Co-chair of VDPAM Advisory Board

ISU College of Vet Med, VDPAM

Ames, IA

Dr. Jodie Pettit Private Practitioner with swine, beef, equine and dairy focus. Co-chair of VDPAM Advisory Board, President-Elect IVMA

Audubon-Manning Veterinary Clinic

Audubon, IA

Dr. Rodger Main Director of Operations, ISU VDL ISU College of Vet Med, (VDL)

Ames, IA

Dr. Pat Gorden Director of Food Supply Veterinary Medicine ISU College of Vet Med, VDPAM (FSVM)

Ames, IA

Dr. Mark Brinkman Private Practitioner with swine, beef, and small animal focus

Veterinary Medical Center

Williamsburg, IA

Christian Davies Accountant and Alpaca Producer C&M Acres Maxwell, IA

Dr. Ann Garvey State Public Health Veterinarian Iowa Dept of Public Health

Des Moines, IA

Dr. John Hicks Private Practitioner with swine and beef focus Carroll Veterinary Clinic Carroll, IA

Dr. Marlin Hoogland Veterinarian, Murphy Brown Western Operations, integrated food supply company

Murphy Brown Orange City, IA

Dr. Gavin Meerdink Toxicologist, former VDL Director University of Illinois Mahoment, IL

Dr. Lisa Nolan Dean of Veterinary Medicine ISU College of Vet Med Ames, IA

Bill Northey Iowa Secretary of Agriculture Department of Agriculture & Land Stewardship

Des Moines, IA

Dr. Jodie Pettit Private Practitioner with swine, beef, equine and dairy focus. Co-chair of VDPAM Advisory Board, President-Elect IVMA

Audubon-Manning Veterinary Clinic

Audubon, IA

Dr. Dave Schmitt/ Dr. Randy Wheeler

State Veterinarian for Iowa - Dr. Schmitt Assistant State Veterinarian - Wheeler

Iowa Department of Agriculture and Land Stewardship

Des Moines, IA

Dr. Don Sievers Practitioner Slater Animal Hospital Slater, IA

Dr. Gene Tinker AFO Coordinator Iowa Dept of Natural Resources

Manchester, IA

Dr. Mahlon "Whitey" Vorhies

Former Director SDSU and KSU VDL, former Department Chair at KSU

Amana, IA


VDL Faculty List

Halbur, Pat Chair of VDPAM and

Executive Director of ISU VDL Administration, Circovirus, Hepatitis E Virus, Swine Respiratory Disease, PRRSV

[email protected]

Main, Rodger Director of Operations Swine Production Medicine and Nutrition [email protected]

Arruda, Paulo Adjunct Instructor Pathology [email protected]

Burrough, Eric Assistant Professor Pathology, Brachyspira, Campylobacter [email protected]

Coetzee, Hans Associate Professor Cyclone Analytical Detection Service [email protected]

Cooper, V.L. Senior Clinician, Pathologist BVD, Beef Cattle, Dairy, Caprine, Equine, Canine, Feline

[email protected]

Ensley, Steve

Senior Clinician, Toxicology Section Leader

Toxicology, Field Investigation of Tox Issues, Drinking Water Quality, Corn co-products

[email protected]

Frana, Tim Associate Professor Bacteriology Section Leader

Bacterial Diseases, Epidemiology, Diagnostics

[email protected]

Gauger, Phil Assistant Professor Pathology Molecular Section Leader

Pathology, Virology, Swine Influenza, PRRSV, PCV2

[email protected]

Harmon, Karen Adjunct Assistant Professor, Molecular Microbiology, HATS Section Leader

Molecular Testing and Characterization of Animal Bacteria and Viruses

[email protected]

Imerman, Paula Clinician Toxicology/Nutrition [email protected]

Janke, Bruce Professor, Pathology Swine Influenza, Enteric Diseases of Pigs, Pathology

[email protected]

Johnson, John Clinician and Serology Section Leader

Development of Serology Tests and Quality Assurance

[email protected]

Kinyon, Joann Adjunct Assistant Professor, Bacteriology/Clinical Microbiology Section Leader

Bacteriology/Clinical Microbiology [email protected]

Madson, Darin Assistant Professor, Pathology Swine Disease Diagnosis, Nursery/Grow-Finish Health

[email protected]

Opriessnig, Tanja Professor, Pathologist Porcine Circovirus, Erysipelas, Hepatitis E virus, Porcine Parvovirus, Swine Respiratory Diseases

[email protected]

Rumbeiha, Wilson Professor Toxicology/Nutrition [email protected] Schwartz, Kent Senior Clinician, Pathology

Section Leader Pathology, Swine and Beef Diagnostics, Evidence-Based Practice

[email protected]

Snider, Doug Veterinary Diagnostician Toxicology [email protected]

Stevenson, Greg Professor Pathology, Swine Diseases, Diag. Medicine in Food Producing Animals

[email protected]

Yoon Kyoung-Jin Professor Viral Diseases, PRRSV, Influenza, PCV2, Development of Diagnostic Tools

[email protected]

Zhang, Jianqiang Assistant Professor Molecular & viral diagnostics [email protected] Zimmerman, Jeffrey Professor, Epidemiologist Ecology of Viral Infections of Swine [email protected]

New VDL Faculty in 2012

Borys , Moira Adjunct Instructor Microbiology [email protected]

Haas, Nathaniel Adjunct Instructor Microbiology [email protected]

Li, Ganwu Research Assistant Professor Microbiology [email protected]

Pillatzki, Angela Veterinary Diagnostician Pathology [email protected]

mailto:[email protected]




















VDL Staff List

Name Degrees Position Date Hired

Abate, Sarah BS Research Associate II 2008

Adams, Carolyn Clerk III 1994

Aust, Ronisue Histo Technician I 2011

Bade, Daniel BS Research Associate I 2011

Barnes, Kimberly BS Research Associate I 2012

Berghefer, Randy AA, BS Manager Information Technology I 1997

Block, Suzanne BS Laboratory Technician III 2002

Boesenberg, Kelly BS, MS Program Manager I 2006

Boor, Jessica BS Quality Assurance Officer 2006

Bower, Leslie BS, MS Research Associate II 2009

Bradner, Laura BS Research Associate I 2013

Branaman, Rosalyn BS Histology Technician II 1988

Brodie, Joseph AA, BLS Assistant Scientist I 2007

Carter, Sylvia BS Laboratory Technician III 1994

Chriswell, Amy BS Assistant Scientist I 1999

Clark, Cheryl Laboratory Technician III 2011

Clauson, Susan Clerk III 1993

Cramer, Susan BS, MS Research Associate I 2011

Crim, Bret BA Systems Analyst I 2012

Cullen, Johnah BS Research Associate I 2012

Deike, Paige BS Research Associate I 2012

Dunn, Derek BS Research Associate I 2011

Dunn, Linda Clerk III 2000

Emge, Patrick BS Laboratory Technician II 2002

Endreland, Luanne Laboratory Technician II 1996

Fenneman, Dawn BS Histo Technician II 2007

Finch, Mary Clerk III 2006

Fligg, Danielle BS Research Associate I 2011

Frisk, David Clerk II 2006

Fulster, Susan BS Laboratory Technician II 1988

Gard, Theresa BS Research Associate I 2007

Gregory, Marissa BS Research Associate II 2008

Grover, Raymond Jr. BA Laboratory Technician III 1997

Gudenkauf, Kylie BS Research Associate I 2015

Hadaway, Hannah Lab Assistant II 2012

Hall, Timothy BS System Support Specialist II 2007

Haney, Devon BS Research Associate II 2007

Heinen, Sheila BS Laboratory Technician III 2001

Heyer, Jessica Lab Assistant II 2012

Holdredge, Jennifer BS Administrative Specialist II 1989

Huffman, Elise BA Lab Technician III 2012

Ivanytska, Olha Research Associate I 2007

Jones, Jenny Clerk III 2012


Kalkwarf, Erin BS Research Associate II 2006

Klein, Brianna Research Associate I 2012

Koeneke, Stacy Clerk II 2011

Kostohryz, Scott BS Research Associate I 2006

Kowalke, Kary Lab Assistant II 2012

Kurth, Lacey BS Research Associate I 2012

Lee, Christine Account Specialist 1996

Lin, Kathy BS Assistant Scientist I 2002

Lueth, Silvano Lab Assistant II 2012

Magtoto, Ronaldo BS, MS, PhD Research Associate I 2007

Matias Ferreyra, Franco DVM Research Associate I 2012

McCammant, Capri Histology Technician II 2005

McDeid, Leona AA, BA Assistant Scientist I 1997

Meraz, Christine Clerk IV 1994

Mindham, Steven BS Systems Support Specialist III 2004

Minor, Ashley BS Research Associate I 2011

Nelson, Nancy AA, BS Laboratory Technician II 1995

Norris, Sheila BS Assistant Scientist II 1992

Novak, Renee AA Program Assistant II 1990

Olson, Linda Clerk III 1995

Peterson, Jacqueline BA Research Associate II 1988

Raymond, Matt BS Research Associate I 2010

Schrunk, Dwayne BS Research Associate II 1997

Schubert, Abby BS Research Associate I 2012

Schuller, Bruce Facilities Coordinator 2005

Schultz, Daniel BS Laboratory Technician III 2001

Schulz, Leanne BS Research Associate I 2011

Schwarz, Brenda BS Laboratory Assistant I 1990

Sickau, Diane BS Research Associate II 1987

Smith, Linda BA Histology Technician I 2007

Sreedharakurup, Premchand BS Systems Analyst II 2011

Stensland, Wendy BS Assistant Scientist I 2001

Thimmesch, Carrie Lab Technician II 2012

Thompson, Curt BS, MBA Assistant Scientist II 2006

Tuttle, Vanna Clerk III 1996

Upchurch, Nancy Laboratory Technician II 2008

Warneke, Hallie BA Research Associate I 2011

Weeda, Matthew BS Research Associate I 2010

Wilson, Kristine Clerk III 2006

Wulf, Larry BS, MS, PhD Assistant Scientist III 2006

Zagar, Rose BS Clerk III 1990


Honors and Awards

Baker, Rodney 2012 Alumni of the Year – Western Kentucky University, Odgen College of Science and Technology Burrough, Eric Boehringer Ingelheim Vetmedica Professorship in Food Animal Infectious Diseases Main, Rodger Alpha Gamma Rho (Iowa State University) Alumni Achievement Award, 2012 Recognizing professional contributions & achievement in agriculture. Yoon, Kyoungjin Taishan Scholar Oversea Distinguished Expert, recommended by Institute of Animal Science and Veterinary Medicine at Shandong Academy of Agricultural Sciences. Pending approval from the Shandong Ministry of Education, China Service Team of the Year Award, College of Veterinary Medicine, Iowa State University Zimmerman, Jeffrey Appointed to National Pork Board Swine Health Committee


New Tests Introduced in 2012 Test name Test department

Decal Histopathology

Immunohistochemistry B-cell, CD79 (VETPATH) Histopathology

Immunohistochemistry Canine CD18 (VETPATH) Histopathology

Immunohistochemistry C-kit (CD117) (VETPATH) Histopathology

Immunohistochemistry Factor VIII (VETPATH) Histopathology

Immunohistochemistry Kappa/Lambda (VETPATH) Histopathology

Immunohistochemistry Melan A (VETPATH) Histopathology

Immunohistochemistry S-100 (VETPATH) Histopathology

Immunohistochemistry T-Cell, CD3 (VETPATH) Histopathology

Immunohistochemistry Vimentin (VETPATH) Histopathology

Influenza A general - PCR Molecular Diagnostics

Influenza A subtype- PCR Molecular Diagnostics

PCR - Applied Biosystems M. hyopneumoniae Molecular Diagnostics

PCR - Bovine Respiratory Bacterial Panel Molecular Diagnostics

PCR - Bovine Respiratory Viral and Bacterial Panel Molecular Diagnostics

PCR - Bovine Respiratory Viral Panel Molecular Diagnostics

PCR - Influenza A - NAHLN surveillance Molecular Diagnostics

PCR - Influenza A Quantitation Molecular Diagnostics

PCR - Influenza A surv subtype Molecular Diagnostics

PCR - Influenza HA/NA gene subtyping Molecular Diagnostics

PCR - Influenza type A NP gene Molecular Diagnostics

PCR - M. flocculare Molecular Diagnostics

PCR - Mycoplasma hyopneumoniae Quantitation Molecular Diagnostics

PCR - TGE Molecular Diagnostics

PCR - TGE and PRCV Molecular Diagnostics

Sequencing and Analysis - Influenza NAHLN surv Molecular Diagnostics

Sequencing and Analysis - PRRSV Molecular Diagnostics

Sequencing and Analysis - PRRSV HI-VOL Molecular Diagnostics

Quantitative Flotation - Modified Wisconsin Parasitology

BVD antigen ELISA (PI) Large EN Serology

BVD antigen ELISA (PI) Serum/EN Serology

SIV Pfizer H1N2 delta 1 Serology

Aflatoxins LC/MS/MS Screen Toxicology

Extraction: Panel Toxicology

Extraction: Single Compound Toxicology

Heavy Metals Panel Feed/Tissue Toxicology

Heavy Metals Panel for Fluid Toxicology

Hormone Free Feed Panel Toxicology

Molybdenum in fluids Toxicology

Selenium GLP Method Toxicology

Vitamin A in serum Toxicology

Vitamin E in serum Toxicology

Vitamin E in Tissue Toxicology

Virus isolation-Swine influenza NAHLN surveillance Virology


CYCADS - New VDL Section Formed CYCADS - Cyclone Custom Analyte Detection Service The CYCADS section of the Veterinary Diagnostic Laboratory at Iowa State University is composed of a board-certified clinical pharmacologist and toxicologists supported by analytical chemists. The group is centered around a laboratory facility with a focus on high throughput analyses intended to quantify exogenous and endogenous compounds (e.g. drugs, toxins and biomarkers) in organic matrices. It provides analytical services in support of multiple sample, known analyte, pharmacological and toxicological research for academic, industry, and government entities. CYCADS has rigorous and robust procedures in place to deal with regulatory scrutiny and has a vast array of developed and validated analytical methods focused on pharmacology.

• 80 existing extraction methods • 250 analyte detection methods • 150 validated immunoassays

Points of Pride 1. The first unit within a Veterinary Diagnostic Laboratory to offer confirmatory drug residue testing

for practitioners. 2. The first unit within a Veterinary Diagnostic Laboratory to offer analytical support for

pharmacology researchers. 3. The first lab to do anabolic steroid testing in horses in the USA. 4. The only US lab that offers steroid profiling by GC/MS. 5. The lab has an assay that can screen for 300 + compounds in a single run. 6. The only lab with a resident board-certified veterinary clinical pharmacologist on staff. 7. Five staff members each with over 20 years racing lab experience. 8. The lab is large enough to offer complete testing capabilities, small enough to offer individualized

customer service to the client.

Dr. Hans Coetzee Dr. Coetzee serves as Section, where he oversees operation of the Racing Chemistry laboratory, conducts pharmacokinetic services and research, and will initiate new services such as antimicrobial residue testing. Dr. Coetzee also teaches clinical pharmacology related topics in the veterinary and graduate curriculum and collaborates on animal welfare-related research and outreach activities in FSVM. Dr. Coetzee earned his veterinary degree from the University of Pretoria, South Africa. After practicing veterinary medicine for several years in Northern Ireland. Dr. Coetzee worked in the private pharmaceutical industry for 2 years before coming to Iowa State University to pursue a PhD in Veterinary Microbiology. He completed his PhD in 2005 and became board certified by the American College of Veterinary Clinical Pharmacology in 2006. Since 2005, Dr. Coetzee has been on the faculty of the College of Veterinary Medicine at Kansas State University (KSU) where he was promoted to Associate Professor and granted tenure in 2010. At KSU, Dr. Coetzee directed a research program focused on discovery of biomarkers of pain and distress and the development of novel pain treatment regimens. Over the past 3 years, Dr. Coetzee has received a substantial amount of competitive funding for his research from the USDA NRI Animal Well Being program and from the pharmaceutical industry. Dr. Coetzee has also demonstrated entrepreneurial success by establishing Pharmacological Clinical and AnalyTical Services Laboratory (PharmCATS) at KSU. This innovative service unit performs contract pharmacological and toxicological studies and provides assay development. Dr. Coetzee has authored or co-authored over 40 peer-reviewed manuscripts and 2 book chapters in the area of clinical pharmacology,food animal production medicine and welfare.

For more information on CYCADS Services, contact Dr. Coetzee at 515-294-7424, or e-mail address: [email protected]. Or see their website at http://vetmed.iastate.edu/diagnostic-lab/cycads


http://vetmed.iastate.edu/diagnostic-lab/cycads


2012 Statistical Data Accessions 2012 Number of Accessions

Total 52,487

January 4,171

February 4,268

March 4,771

April 4,514

May 4,642

June 4,292

July 3,878

August 4,502

September 3,856

October 4,948

November 4,739

December 3,906 2012 Accessions Bacteriology CYCADS

Molecular/ HATS Pathology Serology Toxicology Virology

Total 15,102 64 30,539 12,038 17,142 1,816 2,901

January 1,228 1 2,446 1,023 1,373 146 213

February 1,323 1 2,478 1,081 1,382 160 207

March 1,347 3 2,663 1,043 1,631 230 277

April 1,341 1 2,569 1,026 1,525 163 252

May 1,344 0 2,546 1,051 1,603 141 291

June 1,280 13 2,428 946 1,468 111 197

July 990 7 2,193 740 1,298 124 242

August 1,151 9 2,540 919 1,408 169 273

September 1,049 8 2,232 868 1,313 143 190

October 1,568 8 3,050 1,319 1,436 187 318

November 1,314 4 3,000 1,079 1,506 117 250

December 1,167 9 2,394 943 1,199 125 191 Accession Trends Bacteriology CYCADS

Molecular/ HATS Pathology Serology

Toxicology/ Nutrition Virology

2012 15,102 64 30,539 12,038 17,142 1,816 2,901

2011 14,618 * 24,942 12,725 16,736 1,452 2,203

2010 13,929 * 22,105 10,922 14,711 1,146 2,348

2009 12,940 * 19,606 11,155 15,581 1,007 2,753

2008 13,859 * 21,639 12,628 17,806 889 4,456

2007 16,366 * 30,872 15,582 20,327 1,117 9,982

2006 16,110 * 20,243 15,422 20,524 1,357 16,608 * CYCADS section was newly formed in 2012. See more on page 10.


Tests

2012 Tests Bacteriology CYCADS Molecular/

HATS Pathology Serology Toxicology/

Nutrition Virology

Total 159,009 2,025 194,275 92,503 580,338 7,418 3,839

January 11,697 13,891 7,488 42,639 482 295

February 11,133 14,856 6,306 35,662 274 241

March 13,178 17,703 8,818 50,767 827 355

April 12,590 1,780 14,244 7,495 44,878 777 257

May 15,072 16,010 9,900 54,576 845 571

June 13,374 5 16,155 8,415 48,792 531 296

July 18,053 3 14,910 5,485 39,340 546 239

August 12,818 1 18,757 6,599 43,428 794 357

September 8,129 8 12,526 3,981 34,832 373 225

October 14,616 188 16,820 9,379 61,064 875 293

November 13,534 39 22,549 11,632 68,990 884 411

December 14,815 1 15,854 7,008 55,370 210 299 Submission Trends

Number of Tests Number of Accessions

2012 1,039,407 52,487

2011 928,822 47,609

2010 716,864 41,770

2009 684,957 39,480

2008 1,157,388 41,957

2007 1,039,032 47,957

2006 1,330,084 47,238

2005 1,005,301 38,907


Species Submission Data

2012 Jan Feb Mar Apr May Jun Jul Aug Sep Oct Nov Dec Total

Total 4,171 4,268 4,771 4,514 4,642 4,292 3,878 4,502 3,856 4,948 4,739 3,906 52,487

Count of species 37

Porcine 2,752 2,767 2,916 2,746 2,833 2,665 2,482 2,831 2,493 3,325 3,206 2,555 33,571

Bovine 585 646 848 723 721 627 429 534 509 665 655 539 7,481

Canine 325 294 319 321 293 267 243 320 244 315 300 266 3,507

Avian 243 290 247 263 277 304 339 312 236 271 274 303 3,359

Equine 88 100 246 278 302 228 177 174 177 151 100 64 2,085

Feline 68 44 65 70 87 63 69 94 50 75 74 70 829

Non-Animal 27 23 27 17 23 20 20 62 45 44 39 32 379

Bat 13 10 10 9 29 19 25 77 15 10 13 18 248

Caprine 23 22 26 27 13 24 18 28 21 10 16 18 246

Ovine 11 30 18 21 16 19 19 13 4 10 4 11 176

Unknown 3 6 15 8 6 13 7 12 2 11 17 7 107

Rodent 13 5 5 5 10 8 16 15 4 6 7 2 96

Cervidae 2 3 5 9 3 11 3 5 25 14 6 6 92

Procyonidae 5 8 8 4 9 5 2 2 9 6 9 4 71

Camelid 5 7 3 5 4 2 3 6 3 11 2 2 53

Lagomorpha 4 3 1 1 4 2 3 2 8 1 2 31

Mephitidae 1 2 2 1 3 5 8 2 2 4 30

Mustelid 1 1 2 7 3 1 1 2 1 19

Marsupial 1 2 2 3 2 1 5 1 17

Primate 1 1 3 2 1 1 4 13

Fish 2 2 2 3 1 10

Snake 4 3 2 1 10

Bison 2 1 1 1 3 1 9

Amphibian 1 1 1 1 1 1 2 8

Antelope 3 2 3 8

Bovine Exotic 1 1 1 2 1 1 7

Bear 1 2 1 4

Porcine Exotic 1 1 1 1 4

Reptilian 1 1 1 1 4

Insectivora 1 2 3

Aquatic Mammal 1 1 2

Exotic 1 1 2

Feline Exotic 2 2

Canine Exotic 1 1

Crocodilian 1 1

Equine Exotic 1 1

Ovine Exotic 1 1


Top 10 species 2006 2007 2008 2009 2010 2011 2012 Total Submissions 47,238 47,957 41,957 39,480 41,770 47,609 52,487

Porcine 28,109 27,847 23,575 22,991 24,589 28,933 33,571

Bovine 7,384 8,275 7,479 6,211 6,393 7,329 7,481

Canine 3,677 3,794 3,694 3,665 3,468 3,588 3,507

Avian 2,563 2,750 2,324 1,857 2,813 3,322 3,359

Equine 2,833 2,646 2,235 2,146 2,074 2,125 2,085

Feline 960 984 913 897 783 758 829

Non-Animal 181 198 268 387 364 267 379

Caprine 223 238 241 203 208 241 248

Bat 269 265 293 295 230 230 246

Ovine 223 239 197 161 161 186 176

Porcine

Bovine

Canine

Avian

Equine Feline

Non-Animal

Caprine Bat

Ovine

Top 10 Species Submitted in 2012


Surveillance Testing

2012 Number of tests

Total 40,373

Avian influenza AGID - 1 23,773

Avian influenza AGID - EY 2,100

Avian influenza ELISA 808

Avian Pneumovirus ELISA 132

BSE Surveillance 16

CWD (NVSL) 16

PCR - Avian influenza virus 98

PCR - CSFV surveillance 1,312

PCR - Influenza A - NAHLN surveillance 1,418

PCR - Influenza A surv subtype 212

PRVgB - ELISA SCREEN NAHLN surveillance 9,716

Rivanol Confirmatory Testing 24

Virus isolation - Avian influenza virus 399

Virus isolation - Swine influenza NAHLN surveillance 349


Bacteriology Testing

Bacteriology trends 2007 2008 2009 2010 2011 2012

Antinuclear Antibody 5 2

Bacterial ID 15,968 20,805 17,527 14,196 18,657 22,657

Bacterial ID from mailed culture 272 1,109 1,215 822 430 895

Brachyspira Culture 1,442 3,139 7,215 7,358 8,962 16,157

Campy and Trich Culture 935 1,582 715 1,025 1,309 1,241

Campylobacter Culture 420 506 535 505 906 581

Chlamydia - AgELISA 52 110 70 34 49 8 Clin Micro Culture - Aerobic and

Anaerobic 3,084 3,559 3,192 2,908 1,681 1,709

Clostridium difficile culture 76 74 71 130 246 32

Clostridium difficile toxin A/B - ELISA 459 495 405 526 526 525

Clostridium FA 30 36 45 48 50 66

Coliform Test (Membrane Filter) 328 498 460 48 58 47 Culture of feed for Clostridium

perfringens 3 2 1 2

Culture Saved 958 1,184 1,239 957 940 1,059

Direct Smear 82 68 59 64 65 53

E. coli K99 LA Typing 502 394 100 22 23 3

Enrichments 8,228 8,192 7,489 6,727 8,241 8,582

Fungal Culture 263 341 287 320 301 367

Johne's Liquid Culture 915 965 338 172 719 305

Johne's Liquid Culture pooled 145 82 99 83

Leptospira FA 108 26 8 2 7 Methicillin Resistant Staph Protein

(PBP2A) 28 95

Milk Culture 2,863 11,608 5,274 5,882 7,431 12,783

Milk Culture – Bulk Tank 74 122 189 44 28 4

Mycoplasma Culture 318 879 969 544 337 163

Mycoplasma Id 25 2 1 2

Mycoplasma Milk Culture 3 26 2 4

Parvo Virus - ELISA 4 4

Poultry - Salmonella Environmental 3,353 3,306 4,636 11,896 28,992 23,195

Rheumatoid factor agglutination 1 1

Routine Culture 49,741 41,378 35,836 33,226 46,238 49,911

Salmonella Id 1,539 1,364 979 830 1,291 1,120

Salmonella Identification (NVSL FDA) 124 Serogroup/serotype (APP, Salm,

Strep) 271 239 204 161 107 172

Slant Request 238 330 229 329 240 219 Special Culture (Nasal, Feces, Semen,

Other) 179 757 1,021 1,106 425 3,360

SRID for IgG 25 124 9 8 13

Standard Plate Count 450 152 271 192 455 1,812

Trichomonas Culture 294 171 226 28 180 394


Bacteriology trends 2007 2008 2009 2010 2011 2012

Susceptibilities

Avian MIC 115 102 76 106 94 101

Bovine Mastitis Kirby Bauer 27 30 6

Bovine Respiratory Kirby Bauer 24 19 6 1

Companion/Equine MIC 199 262 437 895 1,605 1,932

Equine Kirby Bauer 209 233 138 128 23 2

E-TEST 66 56 49 61 80 54

Food Animal MIC 8,324 7,879 6,100 5,527 7,782 8,190

Individual Antibiotic 239 680 837 988

Mastitis MIC 108 76 96 105 88 118

Multiresistant Kirby Bauer 125 61 45 41 23 12

Small Animal Kirby Bauer 890 775 691 446 52 7

2012 Salmonella serotypes isolated Avian Bovine

Porcine

Group B

Salmonella typhimurium var 5- 3 7 210

Salmonella derby 2 122

Salmonella agona 1 5 86

Salmonella typhimurium 10 5 53

Salmonella 4,5,12:i: 5 87

Salmonella heidelberg 4 35

Salmonella schwarzengrund 12

Salmonella reading 8

Salmonella brandenburg 6

Salmonella bredeney 3

Salmonella saint-paul 1

Group C1

Salmonella infantis 2 63

Salmonella ohio 25

Salmonella mbandaka 2 14

Salmonella rissen 2 10

Salmonella altona 6

Salmonella tennessee 4

Salmonella litchfield 4

Salmonella choleraesuis_(kunzendorf) 3

Salmonella livingstone 3

Salmonella braenderup 2

Salmonella montevideo 11 2

Salmonella thompson 2 1 2

Salmonella choleraesuis 1

Salmonella hadar 1

Salmonella kentucky 5

Group C2

Salmonella muenchen 1 16

Salmonella manhattan 5

Salmonella bovismorbificans 5

Salmonella newport 7 4


2012 Salmonella serotypes isolated Avian Bovine

Porcine

Salmonella muechen

Group D

Salmonella enteritidis 11 5

Salmonella javiana 1

Salmonella berta 1

Salmonella duisburg 1

Salmonella dublin 28

Group E

Salmonella anatum 1 7 40

Salmonella senftenberg 2 17

Salmonella london 13

Salmonella uganda 3 6

Salmonella krefeld 6

Salmonella give 1 3

Salmonella meleagridis 2 3

Salmonella muenster 1 1 1

Salmonella uganda_var_15+ 1

Salmonella liverpool 1

Group K

Salmonella cerro 5 2

Group Poly/Other Salmonella johannesburg 1 39

Salmonella worthington 22

Untypeable

Salmonella rough O:-:1,5 6

Salmonella rough_O:c:1,5 5

Salmonella 6,7:-:1,5 1 3

Salmonella 6,7:nonmotile 2

Salmonella barranquilla 1 2

Salmonella panama 2

Salmonella 6,7:-:I,w 2

Salmonella 6,7 nonmotile 1

Salmonella bere 1

Salmonella molade 1

Salmonella oranienburg 1

Salmonella rough O:i:z13:1,5 1

Salmonella rough_O:d:lw 1

Salmonella rough_O:eh:1,2 1

Salmonella rough_O:fg:- 1

Salmonella rough_O:i:1,2 1

Salmonella rough_O:z:lw 1

Salmonella soerenga 1

Salmonella 6,7:c:- 1

Salmonella 8,20:nonmotile 1

Salmonella III_38:k:z35 1

Salmonella rough_O:e,h:1,6 1

Salmonella rough_O:gms:- 1


Bacteriology APP serotypes isolated from porcine 2008 2009 2010 2011 2012

APP untypeable 49 43 42 34 68

APP Serotype 7 37 43 35 18 19

APP Serotype 1 7 10 4 4 6

APP Serotype 3 6 8 3 4 6 APP Serotype 12 2 6 1 4

APP Serotype 2 1 2 1 1

APP Serotype 8 3 3 2 1

APP Serotype 11 1

APP Serotype 4 1

APP Serotype 5 29 7 2


Antimicrobial Susceptibility Profiles

• Note: The susceptibility information presented below is a summary of data gathered at ISU VDL for the time period listed. The information may be useful to understand susceptibility trends or as an aid in making clinical decisions, but may not be accurate for specific disease situations.

• In vitro antimicrobial test results do not represent therapeutic recommendations from the VDL or personnel therein. Extra/Off label usage of an

antimicrobial which is limited/prohibited for certain species may result in legal action by FDA-CVM

• Data is reported as: % susceptible (# isolates tested) – not all bacteria isolated at ISU VDL have been tested for antimicrobial susceptibility

Avian 2010-2012 Susceptibility profile of Avian pathogens received at ISU VDL

Data reported as: % susceptible (# isolates tested)1

Antibiotic E coli G ana P mult Salm B Salm D Salm sp

Amoxicillin 79% (143) 100% (10) 100% (3) 96% (23) 100% (22) 91% (32)

Ceftiofur 94% (143) 100% (10) 100% (3) 100% (23) 100% (22) 94% (32)

Clindamycin 0% (143) 0% (10) 0% (3) 0% (23) 0% (22) 0% (32)

Enrofloxacin 97% (143) 100% (10) 100% (3) 100% (23) 100% (22) 100% (32)

Erythromycin 0% (143) 10% (10) 0% (3) 0% (23) 0% (22) 0% (32)

Florfenicol 46% (143) 100% (10) 100% (3) 48% (23) 18% (22) 63% (32)

Gentamicin 81% (143) 100% (10) 100% (3) 96% (23) 100% (22) 78% (32)

Neomycin 87% (143) 70% (10) 100% (3) 96% (23) 100% (22) 91% (32)

Novobiocin 0% (143) 10% (10) 67% (3) 0% (23) 0% (22) 0% (32)

Oxytetracycline 44% (143) 20% (10) 67% (3) 70% (23) 100% (22) 47% (32)

Penicillin 1% (143) 0% (10) 100% (3) 0% (23) 0% (22) 0% (32)

Spectinomycin 2% (143) 0% (10) 0% (3) 0% (23) 0% (22) 0% (32)

Streptomycin 60% (143) 90% (10) 0% (3) 30% (23) 100% (22) 38% (32)

Sulfadimethoxine 53% (143) 70% (10) 33% (3) 17% (23) 32% (22) 41% (32)

Sulphathiazole 62% (143) 40% (10) 33% (3) 70% (23) 95% (22) 69% (32)

Tetracycline 44% (143) 20% (10) 67% (3) 78% (23) 95% (22) 47% (32)

Trimethoprim/Sulphamethoxazole 94% (143) 90% (10) 100% (3) 100% (23) 100% (22) 100% (32)

Tylosin (Tartrate/Base) 0% (143) 10% (10) 0% (3) 0% (23) 0% (22) 0% (32)

2 See Salmonella serotype table on page 18 for most common serotypes isolated within each group


Bovine 2010-2012 Susceptibility profile of Bovine pathogens received at ISU VDL


Antibiotic B tre E coli H som M bov M haem P mult Salm B Salm C2 Salm D Salm sp

Ampicillin 56% (32) 26% (893) 89% (229) 100% (54) 58% (613) 98% (470) 46% (89) 56% (32) 18% (131) 88% (156)

Ceftiofur 88% (32) 54% (893) 100% (229) 100% (54) 99% (613) 100% (470) 74% (89) 56% (32) 20% (131) 90% (156)

Chlortetracycline 50% (32) 11% (893) 98% (229) 100% (54) 62% (613) 94% (470) 36% (89) 38% (32) 3% (131) 72% (156)

Clindamycin 6% (32) 0% (893) 47% (229) 7% (54) 0% (613) 0% (470) 0% (89) 0% (32) 0% (131) 0% (156)

Danofloxacin 34% (32) 50% (893) 74% (229) 2% (54) 54% (613) 82% (470) 75% (89) 81% (32) 82% (131) 79% (156)

Enrofloxacin 47% (32) 60% (893) 78% (229) 100% (54) 57% (613) 90% (470) 96% (89) 100% (32) 100% (131) 99% (156)

Florfenicol 75% (32) 9% (893) 94% (229) 98% (54) 75% (613) 92% (470) 29% (89) 38% (32) 4% (131) 47% (156)

Gentamicin 88% (32) 68% (893) 21% (229) 100% (54) 63% (613) 76% (470) 90% (89) 100% (32) 97% (131) 96% (156)

Neomycin 44% (32) 33% (893) 2% (229) 100% (54) 50% (613) 22% (470) 74% (89) 94% (32) 44% (131) 96% (156)

Oxytetracycline 16% (32) 9% (893) 34% (229) 100% (54) 37% (613) 58% (470) 35% (89) 38% (32) 3% (131) 72% (156)

Penicillin 13% (32) 0% (893) 81% (229) 2% (54) 21% (613) 71% (470) 0% (89) 0% (32) 0% (131) 0% (156)

Spectinomycin 3% (32) 0% (893) 50% (229) 93% (54) 55% (613) 66% (470) 0% (89) 0% (32) 0% (131) 0% (156)

Sulfadimethoxine 38% (32) 11% (893) 12% (229) 98% (54) 13% (613) 7% (470) 2% (89) 6% (32) 2% (131) 29% (156)

Tiamulin 56% (32) 1% (893) 100% (229) 100% (54) 94% (613) 72% (470) 0% (89) 0% (32) 0% (131) 0% (156)

Tilmicosin 47% (32) 0% (893) 90% (229) 98% (54) 44% (613) 66% (470) 0% (89) 0% (32) 0% (131) 0% (156)

Trimethoprim/Sulphamethoxazole 75% (32) 43% (893) 94% (229) 100% (54) 0% (613) 1% (470) 82% (89) 97% (32) 74% (131) 97% (156)

Tulathromycin NI NI 79% (229) NI 58% (613) 83% (470) NI 0% (32) NI NI

Tylosin (Tartrate/Base) 9% (32) NI 59% (229) NI 1% (613) 1% (470) NI 0% (32) NI NI

2 See Salmonella serotype table on page 18 for most common serotypes isolated within each group


Bovine Mastitis 2010-2012 Susceptibility profile of Mastitis pathogens received at ISU VDL


Antibiotic E coli E faem Ente K pneu P mult S aur S dysg S epi S ube

Ampicillin 80% (49) 100% (2) 63% (8) 0% (10) 100% (2) 78% (64) 100% (27) 83% (36) 78% (40)

Ceftiofur 96% (49) 50% (2) 88% (8) 100% (10) 100% (2) 100% (64) 100% (27) 94% (36) 85% (40)

Cephalothin 82% (49) 50% (2) 0% (8) 100% (10) 100% (2) 100% (64) 100% (27) 94% (36) 100% (40)

Erythromycin 0% (49) 0% (2) 0% (8) 0% (10) 0% (2) 97% (64) 93% (27) 83% (36) 75% (40)

Oxacillin 0% (49) 0% (2) 0% (8) 0% (10) 50% (2) 94% (64) 96% (27) 6% (36) 78% (40)

Penicillin 0% (49) 50% (2) 0% (8) 0% (10) 100% (2) 77% (64) 100% (27) 81% (36) 35% (40)

Penicillin/Novobiocin 0% (49) NI NI 0% (10) NI 97% (64) 100% (27) 94% (36) 98% (40)

Pirlimycin 0% (49) 50% (2) 0% (8) 0% (10) 0% (2) 97% (64) 93% (27) 86% (36) 78% (40)

Sulfadimethoxine 43% (49) 0% (2) 0% (8) 30% (10) 0% (2) 88% (64) 59% (27) 69% (36) 0% (40)

Tetracycline 47% (49) 50% (2) 50% (8) 80% (10) 50% (2) 89% (64) 19% (27) 61% (36) 65% (40)

3 Isolates resistant to oxacillin are interpreted as potentially methicillin resistant.


Canine 2010-2012 Susceptibility profile of Canine pathogens received at ISU VDL


Antibiotic B bron E coli E fael E faem Ente K pneu P aer P mult Pseu S aur S can S pint

Amikacin 100% (25) 96% (621) 22% (182) 18% (39) 100% (107) 96% (52) 91% (196) 100% (33) 88% (99) 96% (28) 2% (165) 100% (665)

Amoxicillin/Clavulanic Acid 80% (25) 74% (621) 98% (182) 41% (39) 38% (107) 67% (52) 1% (196) 100% (33) 45% (99) 75% (28) 99% (165) 85% (665)

Ampicillin 28% (25) 58% (621) 96% (182) 38% (39) 39% (107) 13% (52) 0% (196) 100% (33) 41% (99) 32% (28) 98% (165) 47% (665)

Cefazolin 16% (25) 81% (621) 4% (182) 10% (39) 25% (107) 67% (52) 1% (196) 100% (33) 33% (99) 75% (28) 100% (165) 85% (665)

Cefovecin 16% (25) 82% (621) 3% (182) 3% (39) 79% (107) 75% (52) 0% (196) 100% (33) 38% (99) 75% (28) 98% (165) 82% (665)

Cefoxitin 16% (25) 84% (621) 1% (182) 0% (39) 43% (107) 69% (52) 1% (196) 100% (33) 36% (99) 54% (28) 99% (165) 83% (665)

Cefpodoxime 16% (25) 81% (621) 15% (182) 8% (39) 82% (107) 81% (52) 0% (196) 97% (33) 37% (99) 75% (28) 98% (165) 82% (665)

Ceftiofur 12% (25) 81% (621) 4% (182) 10% (39) 82% (107) 81% (52) 0% (196) 100% (33) 38% (99) 75% (28) 99% (165) 82% (665)

Cephalothin 83% (6) 63% (51) 7% (95) 21% (14) 10% (10) 0% (2) 0% (22) 100% (2) 27% (11) 69% (16) 98% (106) 87% (378)

Chloramphenicol 96% (25) 81% (621) 92% (182) 95% (39) 76% (107) 81% (52) 2% (196) 100% (33) 42% (99) 71% (28) 96% (165) 86% (665)

Clindamycin 8% (25) 0% (621) 1% (182) 36% (39) 0% (107) 0% (52) 1% (196) 3% (33) 6% (99) 89% (28) 86% (165) 73% (665)

Doxycycline 100% (25) 77% (621) 72% (182) 46% (39) 64% (107) 73% (52) 6% (196) 97% (33) 65% (99) 86% (28) 62% (165) 59% (665)

Enrofloxacin 92% (25) 86% (621) 32% (182) 0% (39) 93% (107) 87% (52) 45% (196) 100% (33) 59% (99) 82% (28) 46% (165) 77% (665)

Erythromycin 4% (25) 0% (621) 26% (182) 8% (39) 1% (107) 0% (52) 1% (196) 27% (33) 19% (99) 68% (28) 0% (165) 72% (665)

Gentamicin 84% (25) 86% (621) 52% (182) 10% (39) 96% (107) 88% (52) 72% (196) 100% (33) 83% (99) 96% (28) 35% (165) 81% (665)

Imipenem 100% (25) 100% (621) 98% (182) 36% (39) 99% (107) 100% (52) 95% (196) 100% (33) 96% (99) 71% (28) 100% (165) 85% (665)

Marbofloxacin 92% (25) 88% (621) 37% (182) 0% (39) 94% (107) 94% (52) 72% (196) 100% (33) 81% (99) 82% (28) 81% (165) 82% (665)

Oxacillin3 NI NI NI NI NI NI NI NI NI 75% (28) NI 80% (665)

Penicillin 4% (25) 0% (621) 96% (182) 38% (39) 0% (107) 0% (52) 0% (196) 85% (33) 0% (99) 21% (28) 98% (165) 31% (665)

Ticarcillin 60% (25) 64% (621) 8% (182) 18% (39) 76% (107) 17% (52) 89% (196) 100% (33) 72% (99) 75% (28) 99% (165) 83% (665)

Ticarcillin/Clavulanic Acid 96% (25) 77% (621) 7% (182) 15% (39) 87% (107) 81% (52) 89% (196) 100% (33) 78% (99) 75% (28) 99% (165) 84% (665) Trimethoprim/

Sulphamethoxazole 40% (25) 82% (621) 93% (182) 77% (39) 96% (107) 83% (52) 8% (196) 97% (33) 51% (99) 100% (28) 96% (165) 75% (665)



Caprine / Ovine 2010-2012 Susceptibility profile of Caprine / Ovine pathogens received at ISU VDL


Antibiotic B tre E coli M haem P mult Salm sp

Ampicillin 100% (5) 45% (33) 98% (44) 88% (8) 89% (18)

Ceftiofur 100% (5) 82% (33) 100% (44) 100% (8) 94% (18)

Chlortetracycline 40% (5) 12% (33) 95% (44) 88% (8) 83% (18)

Clindamycin 0% (5) 0% (33) 0% (44) 0% (8) 0% (18)

Danofloxacin 80% (5) 79% (33) 93% (44) 88% (8) 72% (18)

Enrofloxacin 100% (5) 82% (33) 98% (44) 88% (8) 94% (18)

Florfenicol 100% (5) 3% (33) 100% (44) 100% (8) 78% (18)

Gentamicin 100% (5) 79% (33) 100% (44) 100% (8) 94% (18)

Neomycin 100% (5) 45% (33) 89% (44) 88% (8) 100% (18)

Oxytetracycline 40% (5) 12% (33) 82% (44) 75% (8) 83% (18)

Penicillin 0% (5) 0% (33) 39% (44) 75% (8) 0% (18)

Spectinomycin 0% (5) 3% (33) 93% (44) 100% (8) 0% (18)

Sulfadimethoxine 100% (5) 18% (33) 57% (44) 13% (8) 6% (18)

Tiamulin 100% (5) 0% (33) 95% (44) 100% (8) 0% (18)

Tilmicosin 100% (5) 0% (33) 98% (44) 100% (8) 0% (18)

Trimethoprim/Sulphamethoxazole 100% (5) 64% (33) 5% (44) 0% (8) 100% (18)

Tulathromycin NI NI 93% (44) 100% (8) 0% (18)


Equine 2010-2012 Susceptibility profile of Equine pathogens received at ISU VDL


Antibiotic A equ B bron P aer Past R equ S aur S equi S equs S zoo Salm B

Amikacin 95% (19) 100% (4) 100% (6) 100% (3) 88% (8) 100% (15) 0% (7) 11% (19) 1% (105) 100% (9)

Ampicillin 100% (19) 25% (4) 0% (6) 100% (3) 13% (8) 67% (15) 100% (7) 100% (19) 96% (106) 89% (9)

Azithromycin NI NI NI 100% (3) NI 93% (14) 100% (6) 100% (19) 97% (100) NI

Cefazolin 89% (19) 0% (4) 0% (6) 100% (3) 13% (8) 100% (15) 100% (7) 100% (19) 99% (105) 89% (9)

Ceftazidime 100% (19) 100% (4) 100% (6) 100% (3) 13% (8) 100% (14) 100% (6) 100% (19) 100% (100) 89% (9)

Ceftiofur 95% (19) 0% (4) 0% (6) 100% (3) 13% (8) 100% (15) 100% (7) 100% (19) 96% (106) 89% (9)

Chloramphenicol 89% (19) 100% (4) 0% (6) 100% (3) 88% (8) 100% (15) 100% (7) 100% (19) 98% (105) 89% (9)

Clarithromycin NI NI NI NI 100% (8) 93% (14) NI NI 0% (100) NI

Doxycycline 100% (19) 100% (4) 0% (6) 100% (3) 88% (8) 87% (15) 100% (7) 89% (19) 79% (105) 89% (9)

Enrofloxacin 95% (19) 100% (4) 83% (6) 100% (3) 38% (8) 93% (15) 0% (7) 74% (19) 21% (106) 100% (9)

Erythromycin 11% (19) 0% (4) 0% (6) 67% (3) 88% (8) 87% (15) 86% (7) 95% (19) 93% (105) 0% (9)

Gentamicin 95% (19) 100% (4) 50% (6) 100% (3) 100% (8) 80% (15) 14% (7) 47% (19) 2% (106) 100% (9)

Imipenem 100% (19) 100% (4) 83% (6) 100% (3) 100% (8) 93% (15) 100% (7) 100% (19) 100% (105) 100% (9)

Oxacillin3 NI NI 0% (6) NI NI 100% (15) NI NI NI NI

Penicillin 0% (19) 0% (4) 0% (6) 0% (3) 0% (8) 73% (15) 100% (7) 95% (19) 96% (106) 0% (9)

Tetracycline 100% (19) 100% (4) 0% (6) 100% (3) 88% (8) 71% (14) 100% (6) 63% (19) 21% (100) 89% (9)

Ticarcillin 95% (19) 75% (4) 67% (6) 100% (3) 13% (8) 93% (15) 100% (7) 100% (19) 100% (105) 89% (9)

Ticarcillin/Clavulanic Acid 95% (19) 100% (4) 67% (6) 100% (3) 13% (8) 100% (15) 100% (7) 100% (19) 100% (105) 89% (9)

Trimethoprim/Sulphamethoxazole 100% (19) 50% (4) 0% (6) 100% (3) 63% (8) 80% (15) 100% (7) 100% (19) 93% (106) 89% (9)



E-test 2010-2012 Susceptibility profile of E-Test pathogens received at ISU VDL


Antibiotic Amy Bact Clos Fus Pec Pes

Cefoxitin 100% (20) 92% (85) 92% (13) 94% (33) 89% (9) 100% (11)

Clindamycin 95% (20) 85% (85) 69% (13) 94% (33) 89% (9) 73% (11)

Metronidazole 70% (20) 95% (85) 92% (13) 100% (33) 100% (9) 82% (11)

Penicillin 90% (20) 67% (85) 92% (13) 91% (33) 100% (9) 91% (11)


Feline 2010-2012 Susceptibility profile of Feline pathogens received at ISU VDL


Antibiotic B bron E coli E fael E faem Ente K pneu P aer P mult Pseu S aur S can S pint

Amikacin 100% (16) 99% (81) 44% (45) 18% (11) 94% (16) 100% (2) 100% (33) 88% (34) 83% (18) 100% (18) 15% (13) 100% (20) Amoxicillin/Clavulanic

Acid 81% (16) 80% (81) 98% (45) 27% (11) 44% (16) 100% (2) 0% (33) 100% (34) 61% (18) 94% (18) 100% (13) 90% (20)

Ampicillin 25% (16) 64% (81) 96% (45) 27% (11) 50% (16) 0% (2) 3% (33) 100% (34) 56% (18) 39% (18) 100% (13) 65% (20)

Cefazolin 0% (16) 94% (81) 2% (45) 0% (11) 19% (16) 100% (2) 0% (33) 100% (34) 33% (18) 94% (18) 100% (13) 90% (20)

Cefovecin 0% (16) 94% (81) 2% (45) 0% (11) 75% (16) 100% (2) 0% (33) 97% (34) 33% (18) 94% (18) 100% (13) 90% (20)

Cefoxitin 0% (16) 93% (81) 0% (45) 0% (11) 38% (16) 100% (2) 0% (33) 97% (34) 50% (18) 61% (18) 100% (13) 85% (20)

Cefpodoxime 0% (16) 93% (81) 16% (45) 0% (11) 75% (16) 100% (2) 0% (33) 97% (34) 39% (18) 89% (18) 100% (13) 90% (20)

Ceftiofur 0% (16) 95% (81) 4% (45) 0% (11) 75% (16) 100% (2) 6% (33) 100% (34) 39% (18) 89% (18) 100% (13) 90% (20)

Cephalothin 0% (3) 82% (11) 4% (27) 25% (4) 0% (1) 0% (5) 100% (9) 33% (3) 90% (10) 100% (6) 86% (14)

Chloramphenicol 88% (16) 93% (81) 93% (45) 100% (11) 75% (16) 100% (2) 3% (33) 100% (34) 56% (18) 78% (18) 100% (13) 100% (20)

Clindamycin 0% (16) 0% (81) 4% (45) 9% (11) 0% (16) 0% (2) 0% (33) 0% (34) 6% (18) 89% (18) 85% (13) 80% (20)

Doxycycline 100% (16) 90% (81) 82% (45) 45% (11) 69% (16) 100% (2) 15% (33) 97% (34) 83% (18) 94% (18) 69% (13) 70% (20)

Enrofloxacin 81% (16) 98% (81) 38% (45) 9% (11) 88% (16) 100% (2) 76% (33) 100% (34) 83% (18) 94% (18) 38% (13) 75% (20)

Erythromycin 0% (16) 0% (81) 20% (45) 9% (11) 0% (16) 0% (2) 0% (33) 12% (34) 28% (18) 50% (18) 0% (13) 60% (20)

Gentamicin 100% (16) 100% (81) 71% (45) 9% (11) 94% (16) 100% (2) 91% (33) 97% (34) 89% (18) 100% (18) 62% (13) 90% (20)

Imipenem 100% (16) 100% (81) 100% (45) 27% (11) 94% (16) 100% (2) 97% (33) 100% (34) 89% (18) 94% (18) 100% (13) 90% (20)

Marbofloxacin 100% (16) 98% (81) 40% (45) 9% (11) 94% (16) 100% (2) 97% (33) 100% (34) 89% (18) 94% (18) 77% (13) 75% (20)

Oxacillin3 NI NI NI NI NI NI NI NI NI 94% (18) NI 90% (20)

Penicillin 0% (16) 0% (81) 96% (45) 27% (11) 0% (16) 0% (2) 0% (33) 65% (34) 0% (18) 33% (18) 100% (13) 40% (20)

Ticarcillin 56% (16) 75% (81) 11% (45) 9% (11) 69% (16) 0% (2) 94% (33) 100% (34) 72% (18) 94% (18) 100% (13) 90% (20) Ticarcillin/Clavulanic

Acid 100% (16) 91% (81) 9% (45) 9% (11) 75% (16) 100% (2) 94% (33) 100% (34) 72% (18) 94% (18) 100% (13) 90% (20) Trimethoprim/

Sulphamethoxazole 75% (16) 95% (81) 93% (45) 82% (11) 94% (16) 100% (2) 18% (33) 94% (34) 72% (18) 100% (18) 100% (13) 75% (20)



Porcine 2012 Susceptibility profile of Porcine pathogens received at ISU VDL


Antibiotic A suis APP B bron E coli Erys H ecol HPS Pmul A Pmul D S suis Salm B2 Salm C12 Salm sp

Ampicillin 95% (257) 90% (107) 5% (652) 32% (154) 94% (18) 27% (1141) 98% (565) 99% (862) 98% (365) 97% (2026) 33% (613) 64% (157) 63% (249)

Ceftiofur 100% (257) 95% (107) 0% (652) 71% (154) 83% (18) 65% (1141) 100% (565) 99% (862) 100% (365) 99% (2026) 79% (613) 76% (157) 80% (249)

Chlortetracycline 98% (257) 89% (107) 99% (652) 16% (154) 11% (18) 8% (1141) 100% (565) 99% (862) 97% (365) 18% (2026) 16% (613) 52% (157) 46% (249)

Clindamycin 0% (257) 2% (107) 0% (652) 0% (154) 67% (18) 0% (1141) 7% (565) 0% (862) 0% (365) 20% (2026) 0% (613) 0% (157) 0% (249)

Enrofloxacin 99% (257) 99% (107) 97% (652) 78% (154) 83% (18) 90% (1141) 99% (565) 100% (862) 100% (365) 97% (2026) 95% (613) 96% (157) 91% (249)

Florfenicol 99% (257) 98% (107) 31% (652) 14% (154) 11% (18) 17% (1141) 99% (565) 100% (862) 100% (365) 98% (2026) 18% (613) 20% (157) 35% (249)

Gentamicin 98% (257) 3% (107) 99% (652) 76% (154) 6% (18) 66% (1141) 83% (565) 98% (862) 99% (365) 96% (2026) 85% (613) 76% (157) 77% (249)

Neomycin 93% (257) 2% (107) 94% (652) 73% (154) 6% (18) 58% (1141) 43% (565) 90% (862) 93% (365) 75% (2026) 79% (613) 84% (157) 81% (249)

Oxytetracycline 79% (257) 16% (107) 98% (652) 14% (154) 11% (18) 7% (1141) 94% (565) 23% (862) 55% (365) 4% (2026) 13% (613) 51% (157) 43% (249)

Penicillin 2% (257) 18% (107) 0% (652) 0% (154) 94% (18) 0% (1141) 25% (565) 87% (862) 97% (365) 81% (2026) 0% (613) 0% (157) 0% (249)

Spectinomycin 0% (257) 6% (107) 0% (652) 1% (154) 72% (18) 2% (1141) 55% (565) 1% (862) 0% (365) 19% (2026) 0% (613) 0% (157) 0% (249)

Sulfadimethoxine 92% (257) 38% (107) 24% (652) 36% (154) 11% (18) 24% (1141) 27% (565) 24% (862) 27% (365) 33% (2026) 3% (613) 32% (157) 8% (249)

Tiamulin 96% (257) 93% (107) 0% (652) 0% (154) 67% (18) 1% (1141) 97% (565) 79% (862) 29% (365) 87% (2026) 0% (613) 0% (157) 0% (249)

Tilmicosin 95% (257) 93% (107) 2% (652) 0% (154) 89% (18) 0% (1141) 90% (565) 92% (862) 43% (365) 24% (2026) 0% (613) 0% (157) 0% (249) Trimethoprim/ Sulphamethoxazole 98% (257) 0% (107) 26% (652) 71% (154) 17% (18) 70% (1141) 92% (565) 0% (862) 0% (365) 97% (2026) 86% (613) 83% (157) 92% (249)

Tulathromycin 0% (257) 99% (107) 98% (652) 0% (154) 0% (18) 0% (1141) 0% (565) 99% (862) 99% (365) 0% (2026) 0% (613) 0% (157) 0% (249)

Tylosin (Tartrate/Base) 0% (257) 2% (107) 0% (652) 0% (154) 0% (18) 0% (1141) 0% (565) 0% (862) 0% (365) 0% (2026) 0% (613) 0% (157) 0% (249)

Carabadox 4 E coli Salm

>2 ug/ml <= 2 ug/ml >2 ug/ml <= 2 ug/ml

35% (596) 65%(596) 15%(324) 85%(324)

2 See Salmonella serotype table on page 18 for most common serotypes isolated within each group 4 A result of <=2 ug/ml for Carbadox is a conservative indicator of bacterial inhibition by this antimicrobial agent. The result shown is based on pharmacokinetic research

indicating an average Carbadox level of 4.5 mcg/ml in the small intestine of pigs fed a dose rate of 50 g/ton. (De Graff 1988).


Key:

1 Data is reported as: % susceptible (# isolates tested) – not all bacteria isolated at ISU VDL have been tested for antimicrobial susceptibility 2 See Salmonella serotype table on page 18 for most common serotypes isolated within each group

3 Isolates resistant to oxacillin are interpreted as potentially methicillin resistant. 4 A result of <=2 ug/ml for Carbadox is a conservative indicator of bacterial inhibition by this antimicrobial agent. The result shown is based on pharmacokinetic

research indicating an average Carbadox level of 4.5 mcg/ml in the small intestine of pigs fed a dose rate of 50 g/ton. (De Graff 1988). 5 Multidrug resistant isolates were found resistant to most classes of antimicrobial in the 1st round of testing. This table represents additional Disk Diffusion

testing for those isolates. NA Not applicable ND Not done NI No interpretation

A equ - Actinobacillus equuli H ecol - hemolytic E.coli S aur - Staphylococcus aureus

A suis - Actinobacillus suis H som - Histoplilus somni S beta- Beta Streptococcus species

Abua - Acinetobacter species HPS - Haemophilus parasuis S can - Streptococcus canis

Amy - Actinomyces species K pneu - Klebsiella pneumoniae S chol - Salmonella choleraesuis

APP - Actinobacillus pleuropneumoniae M bov - Moraxella bovis S dysg - Streptococcus dysgalactiae

B bron - Bordetella bronchiseptica M haem - Mannheimia haemolytica S epi- Staphylococcus epidermidis

B tre - Bibersteinia trehalosi (formerly Pasteurella trehalosi) P aer - Pseudomonas aeruginosa S equi - Streptococcus equi

Bact - Bacteriodes group P cab - Pasteurella caballi S equs - Streptococcus equisimillis

C diff - Clostridium difficile P mult - Pasteurella multocida S pint - Staph pseudintermedius

C perf - Clostridium perfringens Past - Pasteurella species S suis - Streptococcus suis

Clos - Clostridium species Pec - Peptocococcus species S ube - Streptococcus uberis

E coli - Escherichia coli Pes - Peptostreptococcus species S zoo - Streptococcus zooepidemicus

E fael - Enterococcus faecalis Pmul A - Pasteurella multocida Type A Salm sp- Salmonella species

E faem - Enterococcus faecium Pmul D - Pasteurella multocida Type D Salm B - Salmonella species group B

Enc - Enterococcus species Prot - Proteus species Salm C1 - Salmonella species group C1

Ente - Enterobacter species Prp - Propionibacterium species Salm C2 - Salmonella species group C2

Erys – Erysipelothrix Pseu - Pseudomonas species Salm D - Salmonella species group D

Fus - Fusobacterium R equ - Rhodococcus equi Salm E - Salmonella species group E

G ana - Gallibacterium anatis


CYCADS Testing

CYCADS trends* 2007 2008 2009 2010 2011 2012 AB Panel without CA or AMG using LCMS 68

Antibiotic Panel using LCMS 1

Individual Antibiotic using LCMS 171

Meloxicam by LCMS: Research 77

Substance P by I.A.: Research 1,708 * CYCADS section was newly formed in 2012

Molecular and Health Assurance (HATS) Testing

Molecular Trends 2007 2008 2009 2010 2011 2012

PCR Totals 83,761 83,259 83,651 97,521 140,661 173,000 PCR - Actinobacillus pleuropneumoniae 1 3 7 1 8

PCR - Actinobacillus suis 16

PCR - Avian influenza virus 1,270 1,545 1,173 2,163 1,027 68 PCR - Avian paramyxovirus-1 matrix

gene 4 36

PCR - Bovine Coronavirus 901 1,228 1,053 2,273 97 55 PCR - Bovine Respiratory Bacterial

Panel 35 PCR - Bovine Respiratory Viral and

Bacterial Panel 80

PCR - Bovine Respiratory Viral Panel 48 PCR - Brachyspira hyodysenteriae or

B.pilosicoli 4 156 328 453 507 33

PCR - Brachyspira species panel 207 251

PCR - BRSV 160 245 164 240 258 187

PCR - BVDV 120 339

PCR - BVDV fluid 379 255 368 150 89

PCR - BVDV genotyping 9 5 6 1 2

PCR - BVDV tissue/blood 331 229 60 363 146

PCR - Calf diarrhea panel 470 541 637 701 759

PCR - Canine distemper virus 4 9 3 22 9 17

PCR - Caprine herpesvirus 1 2 3 PCR - Clostridium perfringens

genotyping 65 218 183 161 141 127

PCR - CSFV surveillance 2,170 443 1,214 1,155 1,119 1,383

PCR - E. coli genotyping 226 881 731 618 645 679

PCR - Equine adenovirus 1 1

PCR - Equine arteritis virus 4 10

PCR - Equine herpesvirus 1 and 4 6 36

PCR - Equine herpesvirus 2 4

PCR - Equine influenza virus 1 1

PCR - Erysipelothrix 12 67 94

PCR - FMD surveillance 2 4



PCR - Haemophilus parasuis 110 267 475 584 884 1,161 PCR - IBR virus (bovine herpesvirus

type 1) 34 169 174 157

PCR Influenza totals 7,635 6,785 5,850 6,576 15,856 24,783 PCR - Influenza A - Feral swine

surveillance 13 PCR - Influenza A - NAHLN

surveillance 42 48 1,498 1,573

PCR - Influenza A general 7,718

PCR - Influenza A NP gene 311 1,512 1,606 2,037 PCR - Influenza A screen and

subtype 6,581 1,570 4,464 9,721 6,424

PCR - Influenza A subtype 2,593

PCR - Influenza A surv subtype 168

PCR - Influenza HA gene 8 157 287 556 PCR - Influenza HA/NA gene

subtyping 1 46 332 321

PCR - Influenza M gene differential 225 322 21 8 PCR - Influenza pH1N1 differential -

Feral swine surveillance 2 PCR - Influenza pH1N1 differential -

NAHLN assay 1,945 3,000 PCR - Influenza pH1N1 differential -

NAHLN surveillance 9 27 431 385

PCR - Influenza SIV 7,635 3,547

PCR - Influenza SIV HA gene 204 137

PCR - Lawsonia intracellularis 173 384 812 1,052 1,777 1,849

PCR - Leptospira 271 507 372 367 565 472

PCR - Leptospira hardjo-bovis 319 60 11 43 26 1

PCR – M. bovis 79 386 399 387 418 320

PCR - M. flocculare 172

PCR - M. hyopneumoniae 2,422 4,339 6,344 4,472 6,303 1,990 PCR - M. hyopneumoniae Applied

Biosystems 3,951 PCR - M. hyopneumoniae

(mhp165/mhp183) 4

PCR - M. hyorhinis 108 774 906 1,705

PCR - M. hyosynoviae 116 377 716 1,251

PCR - Malignant Catarrhal Fever virus 1 8 6 PCR - Mycobacterium paratuberculosis

(Johne’s) 315 395 284 561 843 PCR - Mycobacterium paratuberculosis

(Johne’s) pool 25 46 137 26

PCR - P. multocida toxin 18 45 34 5 23 25

PCR - PCV fluid 59

PCR - PCV ORF2 27 58 3 15 11

PCR - PCV tissue 207

PCR - PCV2 3,559

PCR - PCV2 fluid 6,315 5,170 3,424 4,022 6,701 6,081

PCR - PCV2 tissue 861 511 386 412 777

PCR - Porcine Cytomegalovirus 21 12

PCR - Porcine Parvovirus 246 269 262 603 1,435 1,827

PCR - Porcine Rotavirus 320 2,039 2,347



PCR - Porcine Teschovirus 47 66 55 49 47 70

PCR PRRS totals 57,400 56,682 56,602 66,385 93,058 112,731 PCR - PRRSV 31,861 34,953 31,571 19,938 3

PCR - PRRSV (HATS) 25,539 20,573 23,730 14,188 48 PCR - PRRSV (HATS) Applied

Biosystems 29,399 44,530

PCR - PRRSV (HATS) Tetra-Core 5,523

PCR - PRRSV Applied Biosystems 44,526 62,551

PCR - PRRSV ORF5 1,156 1,301 3,077 4,996 5,615

PCR - PRRSV Tetra-Core 29,182 8,563 35

PCR - Rabies 2 1 1 2 1

PCR - Salmonella enteritidis 46

PCR - Salmonella spp 36 5 31

PCR - TGE 98

PCR - TGE/PRCV 1,300 1,251 1,068 1,170 1,928 1,516

PCR - Tritrichomonas foetus 169 457 804 1,411 PCR - virulent Newcastle Disease virus

fusion gene 1 17

PCR - WNV 40 12 9 9 3 13

PCR - unspecified 738 493 814 652 283 287

Quantitation Totals 4,335 4,805 7,966 4,964 11,955 15,696 Quantitation - M. hyopneumoniae 1,284

Quantitation - Influenza A 290

Quantitation - PCV2 2,763 2,501 2,521 2,620 9,024 7,782

Quantitation - PRRSV 1,557 2,289 5,419 2,331 2,606 6,340

Quantitation - PRRSV Tetracore 325

Quantitation - unspecified 15 15 26 13

RFLP - PRRSV 2 1

Sequencing Totals 1,598 1,341 1,368 2,625 4,145 5,578 Sequencing - Influenza HA gene 126 8 79 130 242

Sequencing - Influenza NAHLN surv 309 948

Sequencing - SIV 171 5 92

Sequencing - PCV ORF2 4

Sequencing - PCV2 224 21 12 3 12 9

Sequencing - PRRSV 1,173 1,184 1,256 2,543 3,694 4,310

Sequencing - unspecified 30 1 69


Pathology Testing

Pathology Trends 2007 2008 2009 2010 2011 2012

Biopsy Hematoxylin and Eosin Slides 1,231 1,202 1,175 995 1,125 960

Hematoxylin and Eosin Slides 40,811 36,702 33,123 35,175 40,365 42,614

Immunohistochemistry total 78,197 66,471 47,498 40,913 44,206 41,918

IHC - Bovine 2,900 1,931 1,602 2,068 2,031 1,308

IHC - BVD PI Biopsy 55,215 50,495 34,688 29,794 33,993 33,208

IHC - Porcine 19,020 12,576 9,696 8,049 7,794 7,119

IHC - Unclassified 1,062 1,469 1,512 1,002 388 283

Necropsy 2,696 2,758 2,359 5,716 4,217 2,302

Other 6 2 3,197

Parasitology 316 252 364 489 1,833 878

Special Stains 843 476 223 448 907 634 Serology Testing

Serology trends 2007 2008 2009 2010 2011 2012

Anaplasma marginale CARD 1,383 332 22 3 3 2

Anaplasma marginale cELISA 11,454 11,117 7,040 10,394 14,593 19,469

Anaplasma marginale CF 553 499 541 498 777 549

APP totals 8,469 41,610 17,999 9,133 43,311 63,326 APP 1-2-9-11 5,899

APP 3-6-8 172

APP 3-6-8-15 10

APP 3-6-8-15 5,999

APP 4-5-7 5,999

APP 10-12 569 1,334

APP ApxIV Elisa 705 233

APP CF screen (no serotyping) 5,667 22,146 10,228 5,401 19,544 21,762

APP CF serotype 1 only 11 5 5 108 46

APP CF serotype 3 only 2,569 18,688 7,522 3,641 18,139 20,768



APP CF serotype 157 130 15 1 15 16

APP Mix ELISA Screen 76 4,535 1,427

Avian pneumovirus ELISA 4,367 358 81 15 95 113

Baculovirus ELISA 1,300 8,766 5,474

Bluetongue virus AGID 1,024 589 503 950 1,112 205

Bluetongue virus cELISA 124 218 221 165 560

Bluetongue virus VMRD cELISA 818 1,633

Bovine leukemia virus AGID 15,205 13,539 8,578 13,102 17,172 21,075

Bovine leukemia virus ELISA 131 37 351 550

BRSV VN 586 443 173 800 405 180

Brucella totals 30,434 38,308 21,231 25,298 36,787 50,082 Brucella abortus FPA 621 4,124

Brucella BAPA SCREEN 19,608 25,798 12,864 16,900 19,195 24,283


Serology trends 2007 2008 2009 2010 2011 2012

Brucella canis RSAT 404 427 1,290 694 725 304

Brucella CARD test 4,388 6,413 4,244 5,507 8,192 4,966

Brucella RAP 2,845 212 75 4 50 2

Brucella RIV 214 206 33 56 83 51

Brucella SPT 745 638 655 691 775 1,060

Brucella STT 2,230 4,614 2,070 1,446 7,146 15,292

BVD antigen ELISA (PI) 5,535 3,231

BVD antigen ELISA (PI) Large EN 4

BVD antigen ELISA (PI) mod 238 77

BVD antigen ELISA (PI) Serum/EN 5,464

BVD type 1 VN 2,416 2,775 2,019 4,176 2,631 1,839

BVD type 2 VN 1,948 1,863 1,186 2,588 1,821 1,230

Canine herpesvirus VN 2 16 16 31 2

Canine influenza virus H3N8 HI 25 525 144 1

Caprine arthritis encephalitis AGID 387 149 185 199 43 8

Caprine arthritis encephalitis cELISA 534 564 478

Coxiella burnetii IFA 61

EHD virus AGID 611 574 469 525 733 688

EIA virus AGID 2,153 1,776 1,459 1,620 1,480 1,333

EIA virus ELISA 2,361 2,268 2,322 2,306 2,630 2,283

Hemophilus somnus CF 302 185 94 169 68 58

IBR (BHV-1) VN 1,649 2,029 1,211 1,703 1,894 1,397

Influenza totals 99,684 70,249 59,099 61,306 62,411 58,176 Avian influenza AGID 33,127 22,752 17,978 18,739 22,315 26,019

Avian influenza ELISA 481 926 573 626 200 790

Influenza Pandemic H1N1 virus 81 879 660 41

SIV H1 XP-012 (gamma) HI 2,398 1,499 1,856 1,488

SIV H1 XP-031 (delta) HI 528 256 306 120

SIV H1N1 (73) HI 8 106 114 41 3

SIV H1N1 (99) HI 13,193 8,006 5,946 6,789 4,689 3,560

SIV H1N1 Elisa 15,579 12,301 9,425 6,919 7,411 5,853

SIV H1N1 HI 20

SIV H1N1 HI (31) Pfizer 99 83

SIV H1N1 HI Pfizer 4,850 3,025 1,121

SIV H1N2 delta 1 Pfizer 3

SIV H1N2 Human like 2007013281 258 1,098 474 14

SIV H2N9 HI 6

SIV H3 XP-069 (Clus 4) HI 195 48 20

SIV H3N2 Elisa 8,659 8,078 7,158 5,571 6,098 5,069

SIV H3N2 HI 22,606 6,430

SIV H3N2 HI C1/3 7,035 4,965 1,182 7 126

SIV H3N2 HI C4 3,429 5,741 3,889 3,780

SIV H3N2 HI C4 2007013431 99 793

SIV HI Homologous 46 188 14 29

SIV NP Elisa 968 1,490 4,069 11,698 14,397 11,261

SIV NS1 Elisa 155

Johne's totals 27,009 23,631 15,731 16,942 19,692 24,310 Johne’s (bovine) CF 521 486 410 442 536 548


Serology trends 2007 2008 2009 2010 2011 2012

Johne’s (bovine) ELISA 25,938 23,092 15,228 16,458

Johne’s (ovine) AGID 550 53 93 42 105 61

Johne's ELISA - milk 68 92

Johne's ELISA - serum/plasma 18,983 23,609

Lawsonia intracellularis ELISA 661 990 1,682 1,344

Leptospira 5 MAT 2,679 3,093 1,667 1,973 2,233 2,407

Leptospira 6 MAT 3,466 3,765 2,309 1,833 1,476 1,147

Leptospira australis MAT 288 355 276 317 318 301

Leptospira autumnalis MAT 28 90 54 28 45 36

Leptospira ballum MAT 288 355 276 316 318 301

Leptospira bratislava MAT 107 364 35 28

Leptospira canicola MAT 63 108 212

Leptospira grippotyphosa MAT 1

Leptospira hardjo MAT 647 593 25 442 155

Leptospira ictero MAT 18

Leptospira pomona MAT 5 25 55

Leptospira sejroe MAT 291 739 276 316 318 301

Leptospira tarassovi MAT 288 355 276 316 318 301

M hyopneumoniae totals 32,112 44,062 43,461 45,931 50,880 47,160 M hyopneumoniae CF 166

M hyopneumoniae DAKO ELISA 2,950 12,008 12,663 5,027 3,711 1,954

M hyopneumoniae ELISA 29,162 27,637 22,752 31,165 36,754 31,053

M hyopneumoniae Tween 20 4,251 8,046 9,739 10,415 14,153

Neospora caninum ELISA 11,769 10,040 5,230 57

Neospora caninum VMRD cELISA 37 8,458 11,803 15,515

Ovine progressive pneumonia AGID 60 31 72 75 38 49

Pandemic H1N1 virus 41

Parainfluenza 3 Virus VN 602 358 95 460 281 388

Porcine circovirus totals 42,684 39,166 24,490 15,195 16,184 22,245 PCV 2 - 4 dilution 4,100 4,051 4,935 4,921 8,745

PCV 2 - cELISA 36,522 9,376 12,327

PCV 2 - Differential ELISA 12,453 8,529 5

PCV 2 - ELISA 10,043 10,964 8,807 9,339

PCV 2 - FFN 605 242 787 90 1,692 699

PCV 2 - IFA endpoint titration 183 81 1,358 232 474

PCV 2 - IFA screen 3,574 934 78

PCV 2 - IFA titer 1,557 1,787

PCV 2 - IgG Elisa 48

PCV 2 - IgM Elisa 426

PPV HI 3,989 3,000 2,644 1,625 2,712 2,012

PRRS totals 190,004 247,029 126,723 132,539 149,089 157,561 PRRS ELISA 157,031 146,489 119,150 33,588 72 PRRS ELISA (meat juice

surveillance) 27,993 96,770

PRRS FFN 444 325 181 6 9 395 PRRS Homologous IFA North

American 240 PRRS Homologous IFA titration

North American 4


Serology trends 2007 2008 2009 2010 2011 2012

PRRS IFA 2,919 606

PRRS IFA European 1,616 1,379 2,491 2,906 1,509 1,191

PRRS IFA North American 1,456 3,015 3,828 2,811 1,756

PRRS IFA North American titration 3 3 1

PRRS IFA titration 1 1

PRRS Oral Fluid Antibody 1,883 1,922 444

PRRSX3 ELISA 90,045 143,115 146,560

PRRSX3 Oral Fluid Antibody 1,129 7,658

PRV totals 184,628 92,193 32,628 33,179 43,752 34,436 PRV ALA SCREEN 1,773 2,114 1,978 1,466 1,127 8

PRV ELISA SCREEN

PRV g1 ELISA 178,017 83,115 23,663 15,508 16,515 2,159

PRV gB ELISA SCREEN 1,900 1,467 2,269 2,418 6,463 17,380 PRV gB ELISA SCREEN NAHLN

surveillance 9,631 10,347 9,716

PRV VN 2,938 5,497 4,718 4,156 9,300 5,173

Pullorum - typhoid AGG 32 333 1 5 56

TGE virus VN 2,213 7,475 3,335 1,399 6,749 14,644

TGE/PRCV 14,968 13,530

TGE/PRCV Biovet 32 16

TGE/PRCV Heated 8,976 10,349 11,467 12,630

Toxoplasma gondii ELISA 74,659

Toxoplasma LAT 420 235 548 992 615 573

Trichinella spiralis ELISA 74,708

VS virus Indiana strain VN 1,951 1,442 1,834 3,017 2,132 1,590

VS virus New Jersey strain VN 1,950 1,442 1,835 2,996 2,132 1,590

West Nile Virus Capture IgM ELISA 69 43 56 30 76 *BVD PI previously tested in Virology Toxicology/Nutrition Testing

Toxicology/Nutritinon testing trends 2007 2008 2009 2010 2011 2012

Ammonia Nitrogen 21 15 19 12 19 21

Antibiotics - Ampicillin 1

Antibiotics - Chlortetracycline 140 83 93 78 115 31

Antibiotics - Lincomycin 2

Antibiotics - Oxytetracycline 6 6 2 5 2 15

Antibiotics - Penicillin 6 3 18 8 3 3

Antibiotics - Tetracycline 4 15 13 122 7 15

Antibiotics - Tylosin 130 81 75 70 52 13

Antibiotics - Virginiamycin 8

Anticoagulant - Brodifacoum 1 6

Anticoagulant - Bromodiolone 3



Anticoagulant - Dicoumarol 1 1 1 1 5

Anticoagulant - Diphacinone 2 4

Anticoagulant - Warfarin 1

Anticoagulant Panel 18 14 9 19 20 23

Arsenic in feed or tissue 7 16 12 18 10 16

Arsenic in fluids 7 2 1

Ash 13 11 1 15

Barbiturates

Bone Ash 22 16 8 73 309 332

Bone Density 19 12 4 69 250 306

Bone Profile 8 98

Cadmium in feed or tissue 4 10 18

Cadmium in fluids 6

Calcium in feed or tissue 26 13 10 30 53

Calcium in serum, plasma, eye, water 1 3 2

Carbamate 1 1

Carbamate - quantitation 5 3

Carbamate Panel 14 23 14 7 6 1

Carboxyhemoglobin 2 5 5 6 13 10

Chloride in feed or tissue

Chloride in fluids 7 2

Chlorinated Hydrocarbon Panel 3 2 2 3 1

Cholinesterase 34 91 65 35 48

Cholinesterase in blood 15

Cholinesterase in brain 12

CNS Canine Panel 2 2

Copper in blood

Copper in Feed or Tissue 185 156 167 32 61 48

Copper in liver biopsy 11 4 4

Copper in serum or water 132 27 23 19 16 3

Coumestrol - quantitation 1 3

Custom Chemistry 92 131 134 149 623 576

Cyanide 2 3 5 2 3 10

Ergopeptine Panel 12 6 9 1 4 28

Ergotamine 1

Ergovaline 35 46

Ethylene Glycol 17 5 3 3 1

Extraction: Panel 24

Extraction: Single Compound 28

Feed Particle Size 5 4 6



Fluoride 1 18 20 4 5

Fluoride in tissue 2 2

Fumonisins LC/MS/MS Screen 4

GCMS Screen 4 38 28

GCMS Single Compound 3 2

Gossypol 5 1 2 2 2

Gossypol - quantitation 2 1 1 2 5

Heavy Metals Panel for feed or tissue 42

Heavy Metals Panel for fluid 1 Hormonally active feed compounds

panel 2

Hormone Free Feed Panel 11

Ionophores Screen 3 43

Iron in feed or tissue 28 26 20 3 17 26

Iron in liver biopsy

Iron in serum or water 48 14 10 10 16 1

Lasalocid 15 16 14 2 6

Lasalocid 1ppm LOD 1 49

Lead in blood or water 55 44 57 30 414 163

Lead in feed or tissue 36 36 64 44 96 61

Magnesium in feed or tissue 1 2 1 6

Magnesium in fluids 1 3

Manganese in feed or tissue 92 75 11 7

Manganese in serum or water 14 2 2

Melengesterol Acetate 8 5 12 9 3 1

Mercury in blood 1 2 1 1

Mercury in feed or tissue 6 2 3 8 10

Methemaglobin 28 5

Microtox Test

Moisture 71 94 34 22 36 232

Molybdenum in feed or tissue 4 3 1

Molybdenum in fluids 7

Monensin 76 34 20 19 17

Monensin - quantitation 22 17 8 3

Monensin 1ppm LOD 1 67

Mycotoxin - individual - quantitation 21 32 90 31

Mycotoxin - Aflatoxin - qualitative 6 11 10 7 1 Mycotoxin - Aflatoxin - qualitative M1

Liver 4 1 3 11 7 21

Mycotoxin - Aflatoxins ELISA 43 33 Mycotoxin - Aflatoxins LC/MS/MS

Screen 53



Mycotoxin - Fumonisin B1 - qualitative 12 13 3 1

Mycotoxin - Fumonisins ELISA 1 5

Mycotoxin - Ochratoxin - qualitative 1 6

Mycotoxin - T-2 toxin - qualitative 8

Mycotoxin - Tremorgen panel 2 2 5 8 6

Mycotoxin - Vomitoxin - qualitative 2 25 29 17

Mycotoxin - Vomitoxin ELISA 3 18

Mycotoxin - Vomitoxin LC/MS/MS 3

Mycotoxin - Zearalenone ELISA 1 10 1 Mycotoxin - Zearalenone LC/MS/MS

Screen 1 Mycotoxin - Zearalenone/Zearalenol -

qualitative 3 13 14 4 5

Mycotoxin DDGS Panel 2 10

Mycotoxin ELISA Screen 31 Mycotoxin ELISA Screen 1 - includes 2

mycotoxins 1 7 2 Mycotoxin ELISA Screen 2 - includes 3

mycotoxins 30 2 2 Mycotoxin ELISA Screen 3 - includes 4

mycotoxins 123 183 9 15

Mycotoxin Panel 132 73 153 61 23

Mycotoxins LC/MS/MS Screen 98

Nitrate/Nitrite in feed 182

Nitrate/Nitrite in fluids 1 181

Nitrates in feed 8 18 4 5 29

Nitrates in serum or eye fluid 43 27 36 25 136

Nitrite analysis 3

Nitrite or Nitrate in water 11 13 11 8

Organophosphate - qualitative 2 1 3 4 4

Organophosphate - quantitation 5 2 2 6 2

Organophosphate Panel 19 24 10 10 5 1

pH 8 12 20 13 15 31

Phosphorus in feed or tissue 16 12 8 33 53 35

Potassium in feed 1

Potassium in fluids

Selenium GLP Method 5

Selenium in blood, serum or plasma 214 149 79 93 318 229

Selenium in tissue or feed 303 316 199 112 87 704

Slaframine 2 2 6 3

Sodium in feed or tissue 14 41 11 16 31 17

Sodium in fluids 2 6 9

Strychnine 8 1 2 2 1



Sulfa Drug Panel 1 4 1

Sulfate in feed 63 65 27 18 40 27

Sulfate in water 27 28 20 10 13 16

Sulfonamide - qualitative 3

Total Dissolve Solids 3 2 1

Trace mineral panel - bovine liver 66 255 197

Trace mineral panel - bovine serum 50 186 151

Trace mineral panel - feed 64 53 40 118

Trace mineral panel - porcine liver 55 221 282

Trace mineral panel - porcine serum 52 80

Trace mineral panel - serum, water 131 221 27 63

Trace mineral panel - tissue 287 376 50 92

Urea 10 1 5 1

V9 mineral panel 58 29 12

Vitamin A acetate 1 18 16

Vitamin A in serum 14 9 14 59 218 533 Vitamin A palmitate in feed -

quantitation 4 2

Vitamin A palmitate in liver - quantitation 14 4 6 11 48 91

Vitamin D3 in feed 1 6 4

Vitamin E acetate in feed - quantitation 3 11 25 8 2 19

Vitamin E in serum 289 71 66 400 780 1259

Vitamin E in tissue 235 175 325 264 280 304

Water Quality Panel 42 35 55 40 54 67

Zinc in feed or tissue 30 31 9 3 15

Zinc in liver biopsy

Zinc in serum or urine 17 10 7 4 8 4

2012 Toxicology diagnosis Avian Bovine Canine Caprine Equine Non-

Animal Ovine Porcine Grand

Total

Water deprivation 8 8

Lead 1 6 7

Copper 1 3 4

Ethylene glycol (antifreeze) 4 4

Ionophore 4 4

Vitamin D 4 4

Idiopathic 1 2 3

Nephropathy,Undetermined 1 1 2

Ammonia 2 2

Nitrate 2 2

Hepatitis 1 1

Myopathy, Toxic, Misc 1 1


2012 Toxicology diagnosis Avian Bovine Canine Caprine Equine Non-

Animal Ovine Porcine Grand

Total

Algae 1 1

Diphacinone 1 1

Monensin 1 1

Organophosphate 1 1

Oxalate 1 1 Urea 1 1

Vitamins 1 1

Japanese yew (Taxus sp) 1 1

Zinc 1 1 Virology Testing

Virology trends 2007 2008 2009 2010 2011 2012

Antigen ELISA total 5,970 6,090 4,684 1,578 24 14

AgELISA - BVD virus 3,824 3,831 3,237 1,110 3

AgELISA - BVD virus modified 146 156 163 29

AgELISA - Influenza A virus 4 6 4 6

AgELISA - Parvovirus 13 11 13 4 15 7

AgELISA - Type A Rotavirus 1,983 2,086 1,267 429 6 7

AgELISA - unspecified

Fluorescent antibody total 740 556 469 317 306 257

FA - Border disease virus 3

FA - Bovine adenovirus 2

FA - Bovine coronavirus 2

FA - Bovine herpesvirus type 1 71 87 51 13 3

FA - Bovine parainfluenza virus type 3 10 6 3 6 1

FA - Bovine respiratory syncytial virus 4 10

FA - Bovine viral diarrhea virus 4 10 1

FA - Canine adenovirus 19 29 18 11 23 13

FA - Canine coronavirus 7 12 20 17 5 2

FA - Canine distemper 57 65 39 39 42 22

FA - Canine herpesvirus 44 46 39 24 39 30

FA - Canine parvovirus 64 76 98 91 50 43

FA - Encephalomyocarditis virus 1 5 4 1 5

FA - Equine herpesvirus type 1&4 27 21 18 11 11 3

FA - Equine herpesvirus type 3 13 1 1

FA - Feline calicivirus 21 15 7 6 8 3

FA - Feline coronavirus 1 1 1 2

FA - Feline herpesvirus 19 15 8 7 9 3

FA - Feline infectious peritonitis virus 22 7 3 2 2

FA - Feline parvovirus 19 12 8 4 8 7 FA - Hemagglutinating encephalomyelitis

virus 3 18 11 11 5


FA - Porcine enterovirus & teschovirus 12

FA - Porcine parvovirus 41 19 21 23 1 46

FA - Porcine respiratory coronavirus 1

FA - Pseudorabies virus 58 38 79 8 33 17

FA - TGE 12 2 3 1

FA - unspecified 234 76 28 40 51 40

Rabies 1,031 1,049 997 822 783 842

RFLP 4 4

Virus isolation total 1,254 1,365 905 1,058 2,269 2,264

VI - Avian influenza virus 100 258 98

VI - Border disease 1

VI - Bovine parainfluenza virus type 3 1

VI - Bovine viral diarrhea virus 157 383 140 156 262 510

VI - Canine adenovirus 2

VI - Canine distemper virus 2

VI - Canine herpesvirus 3 7 4 2 1

VI - Canine parvovirus 4

VI - Encephalomyocarditis virus 2 7 6

VI - Equine herpesvirus type 1&4 9 5 7 6

VI - Equine influenza virus 1 2

VI - Feline calicivirus 1 3 1 1 6 2

VI - Feline herpesvirus 1 3 2 2 7 2

VI - Feline parvovirus 1 1

VI - Feral swine influenza surveillance 2

VI - IBR virus (BHV-1) 59 177 49 5 3 5

VI - Porcine circovirus type 1 1

VI - Porcine circovirus type 2 117 12 15 2 7 21

VI - Porcine enterovirus 23 14 19 7 2

VI - Porcine parvovirus 1 1 1

VI - Porcine respiratory coronavirus 3

VI - PRRS virus 317 258 307 333 588 419

VI - Pseudorabies virus 32 42 75 7

VI - Swine influenza NAHLN surveillance 434 529

VI - Swine influenza virus 409 388 249 360 645 665

VI - unspecified 121 58 28 80 48 8

Virus propagation 5 13 10 1 1

Virus quantitation 1 7

Virus return 224 181 122 148 331 424


Rabies

2012 Rabies Accessions by month Jan Feb Mar Apr May Jun Jul Aug Sep Oct Nov Dec Total

Grand Total 52 41 65 66 101 71 82 130 53 59 65 57 842

Bat 12 10 7 9 25 15 25 77 14 10 13 18 235

Feline 11 7 16 15 17 21 20 21 8 14 13 14 177

Canine 15 8 16 16 18 14 18 15 11 12 15 11 169

Bovine 6 6 11 15 19 5 3 5 3 5 8 2 88

Raccoon 5 8 8 4 9 5 2 2 9 6 9 6 73

Skunks 1 2 2 1 2 5 8 2 2 4 29

Rodent 1 1 4 5 1 3 4 2 3 24

Equine 3 2 3 1 2 1 3 4 2 21

Ovine 1 2 1 1 2 7

Caprine 1 2 1 1 1 6

Marsupial 1 1 3 5

Cervidae 2 2

Bovine Exotic 1 1

Camelid 1 1

Canine Exotic 1 1

Mustelid 1 1

Ovine Exotic 1 1

Porcine Exotic 1 1

2012 Rabies accessions Positive Negative Inconclusive Unsuitable Total

Grand Total 25 781 28 8 842 Bat 11 224 235

Feline 175 2 177 Canine 159 8 2 169

Bovine 5 71 11 1 88 Procyonidae 67 4 2 73

Mephitidae 9 18 1 1 29 Rodent 24 24

Equine 20 1 21

Ovine 6 1 7 Caprine 6 6

Marsupial 4 1 5 Cervidae 2 2

Bovine Exotic 1 1 Camelid 1 1

Canine Exotic 1 1 Mustelid 1 1

Ovine Exotic 1 1 Porcine Exotic 1 1


Rabies Positive Trends 2005 2006 2007 2008 2009 2010 2011 2012

Total rabies submissions 1095 975 1031 1049 997 822 783 842 Total positives 101 41 22 17 29 20 19 25

% positive 9% 4% 2% 1.60% 3% 2% 2% 3%

Positive animals Bat 43 19 7 6 6 7 7 11 Skunk 29 12 4 4 12 10 7 9 Bovine 6 2 1 4 1 4 5 Feline 21 5 6 5 4 1 1 Canine 2 2 4 1 2 1 Equine 1 1 1

0

20

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100

120

140

Jan Feb Mar Apr May Jun Jul Aug Sep Oct Nov Dec

2012 Rabies Submissions to ISU VDL

Cases per month

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600

800

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2005 2006 2007 2008 2009 2010 2011 2012

Rabies Submissions to ISU VDL

Total number of rabies cases

Number of positive cases


Glossary

AgELISA Antigen-capture ELISA AGG Agglutination AGID Agar gel immunodiffusion AIV Avian influenza virus ALA Automated latex agglutination BAPA Buffered acidified plate antigen test BRSV Bovine respiratory syncytial virus BSE Bovine spongiform encephalopathy BVDV Bovine viral diarrhea virus cELISA Competitive (blocking) ELISA CF Complement fixation CSF Classical Swine Fever ELISA Enzyme-linked immunosorbent assay FA Fluorescent antibody FAD Foreign Animal Diseases FATS Fluorescent antibody tissue section FFN Fluorescent focus neutralization HI Hemagglutination inhibition IFA Indirect fluorescent antibody IHC Immunohistochemistry IPT Immunoperoxidase test LAT Latex agglutination MAT Microscopic agglutination test NADC National Animal Disease Center (USDA:ARS) NVSL National Veterinary Services Laboratories (USDA:APHIS) PCFIA Particle concentration fluorescence immunoassay PCR Polymerase chain reaction PCV Porcine circovirus PI Persistent infection PPV Porcine parvovirus PRCV Porcine respiratory coronavirus PRRS virus Porcine reproductive and respiratory syndrome virus PRV Pseudorabies (Aujeszky’s disease) virus RAP Rapid automated presumptive test RFLP Restriction fragment length polymorphism RIV Rivanol precipitation – plate agglutination test RSAT Rapid slide agglutination test SIV Swine influenza virus SPT Standard plate agglutination test STT Standard tube agglutination test TGEV Transmissible gastroenteritis virus VDL Veterinary Diagnostic Laboratory, Iowa State University VDPAM Department of Veterinary Diagnostic and Production Animal Medicine VI Virus isolation VN Serum-virus neutralization

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