Introduction

The ViewLux® is the gold standard, ultra high-speed microplate imager, ideal for high throughput screening of compound libraries requiring the ultimate in speed, reliability and high quality data.

In fact, over 150 ViewLux systems have been installed in all major pharmaceutical and many biotech companies worldwide.

The ViewLux system features telecentric lens technology which maximizes light collection from the sample plate and increases sensitivity while decreasing parallax error that can result from wells being at different distances from the lens.

Because the ViewLux imager generates both image and numerical data, it provides users with additional insight into their biology.

The ViewLux imager now features the next generation of low-maintenance Peltier-cooled, CCD camera technology that eliminates the need for a cryogenic cooling system. The camera is designed for more sensitive detection, maximum reliability and easy maintenance.

ViewLux uHTS Microplate Imager

Multimode Detection

P R O D U C T N O T E

Key Features

• Unrivalledthroughputofupto200,000samplesperhourundercontinuous operation

• Nextgeneration,low-maintenance,Peltier-cooled CCD camera

• Supports5keynon-radiometrictechnologiesand a wide variety of radiometric assay types

• Roboticloadingand/orplatestackeroptions

• Measuresanysampleinamicroplateformator footprint (e.g.gels,blotmembranesandslides)

Standard camera lens

Telecentric lens

A telecentric lens has no parallax error and no loss of photons due to geometry and wall shading.

The ViewLux imager provides similar performance to a PMT based reader (A), and its imaging capability delivers additional visual data (B) for assay development applications.

A B

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Latest generation camera system

• Fast cooling with easy switch off when not in use – ready to use in 30 minutes after restart

• Compact – no separate camera controller, the cooling system is integrated into the instrument

• Easy maintenance – no vacuum pumping, no heated tubes

• Lower background than ever before – more sensitive detection limit

• Comparable in performance to previous generations of ViewLux readers for maximum confidence and transfer-ability

Radiometric capability

The ViewLux uHTS Microplate Imager can also read a variety of homogeneous radiometric assays such as Scintillation Proximity Assays (with red-shifted SPA beads), Image FlashPlates™, ADME Tox and environmental FATE analysis of HPLC fractions in LumaPlates™.

Work the same way with all assay types

The ViewLux imager can read all commonly used non-radio-metric labels. To change from one technology to another, simply place the appropriate barcode on the microplate and the ViewLux system will read it, whether it is on the long or short edge of the plate. With the one imaging platform you can work in the same way with all your labels and assay types, reducing learning curves, saving space and maximizing the use of robotic automation systems.

A ViewLux system designed for the way you work

A modular design means that the ViewLux imager can keep up with changes in your application or workflow without major reinvestment.

Options include:

• Single or double 32-shelf stacker

• External platform for robotic loading

• Combination 32-shelf stacker and external platform for robotic loading

Choose the best detection for your assay

The system performs luminescence, fluorescence, time-resolved fluorescence, absorbance and fluorescence polarization which makes it perfectly suited to a wide variety of today’s fastest non-radioactive and radioactive assay technologies. The system shows excellent correlation between results from 384- and 1536-well plates, allowing easy assay miniaturization, and with the ability to read a 1536-well plate in 30 seconds, the system can manage throughputs in excess of 200,000 samples per hour under continuous operation. The ViewLux imager can also handle plates in a 3456-well format.

Dual luminescence

The ViewLux uHTS Microplate Imager excels in high throughput, dual luminescence experiments, particularly those requiring only a single reagent such as Chroma-Luc™ (Promega Corporation). Its CCD camera design delivers simultaneous measurement of 1536 wells at each wave-length, so avoiding the signal decay associated with PMT based readers due to measurement time across the plate.

The 3 graphs above show the close correlation in performance between 384- and 1536-well for TRF, luminescence and fluorescence technologies on the ViewLux imager, allowing confident assay miniaturization.

Time-Resolved Fluorescence

Luminescence

Fluorescence Intensity

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Specifications

Power requirements (instrument, PC and cooler)

Standby: 1200 VA

Running: 1800 VA

Maximum: 2000 VA

Power requirement for the instrument only: 1000 VA

Mains power supply: 100-230 VAC, 50/60 Hz, nominal (operating range 90-253 V)

ViewLux fuses: 2 x T8 A H 250 V, for mains voltages of 220-230 V 2 x T15 A H 250 V, for mains voltages of 100-120 V

Safety standards

Certification: IEC-CB, CE and NRTL-TUV Rheinland of North America

The instrument fulfills the requirements of:

IEC 61010-1:2001, UL 61010-1:2004 R10.08, CAN/CSA C22.2 No. 61010-1-04+GI1 (R2009), IEC 61010-2-010:2003, CAN/CSA C22.2 No. 61010-2-010 (R2009)

Conformance to EU directives

The CE mark conforms to the following EU directives: 89/336/EEC (as amended by 92/31/EEC) relating to Electromagnetic Compatibility 2006/95/EC (Low Voltage Directive)

Performance specifications used to verify conformance to the directives above:

EN 55022 class B

IEC 61000-4-3/EN 61000-4-3 3 V/m

IEC 61000-4-2/EN 61000-4-2 4 kV CD, 8 kV AD

IEC 61000-4-4/EN 61000-4-4 1 kV

IEC 61010-1/EN 61010-1

Including Amendments 1 and 2

Reading time with the ViewLux imager: 4 mins/384-well plate.

Reading time with the TopCount system: 6 hrs/384-well plate.

CASE STUDY: ViewLux Imager vs TopCount for 14C Radioisotope Detection in Biological SamplesAn example of how a lower throughput application can be improved using the ViewLux system is shown above. The TopCount™ plate-based scintillation counter is commonly used to generate a chromatogram from HPLC fractions of separated metabolites. While highly sensitive in comparison to the usual method of radio-HPLC detection, the read time in this case is 6 hours. Using the ViewLux imager, the same result is achieved in 4 minutes with minimal loss of signal to background.

Technical design of ViewLux uHTS Microplate Imager

Specifications, continued

Environmental conditions

Operating conditions: +15 °C to +35 °C, Relative humidity 60% maximum at 28 °C, decreasing linearly to 40% at 35 °C (indoor use)

Transportation conditions: 0 °C to +60 °C, Relative humidity 5 to 90%, IEC 68-2-56 as guidelines packed in transportation package

Storage conditions: 0 °C to +60 °C, Relative humidity 5 to 90%, IEC 68-2-56 as guidelines packed in transportation package

Light source

Light source comprising up to 8 flash lamps, spectral range: 300 nm-600 nm.

Rotatable excitation filter wheel with 10 filter positions (Ø 60 mm). Filters can be changed by the user as required.

Detection unit

850 model back-illuminated CCD camera

Rotatable emission filter wheel, with 10 filter positions (Ø 60 mm). Filters can be changed by the user as required.

A TRF-shutter is used in TR-fluorescence to provide a gating window.

Plates

Up to 3456-well microtitration plates are compatible with the instrument.

The user can define non-standard plate configurations. The minimum outer dimensions are 85x127x0 mm and the maximum outer dimensions are 89x129x25 mm.

Measurement time

Detection time per well is software selectable from 0.1 seconds upwards.

Physical dimensions

Weight of basic unit: 350 kg; Overall dimensions: 610x960x1800 mm (WxDxH)

Software

The instrument program is run on a Windows® XP based computer, minimum 256 MB memory, equipped with a CD-ROM, super VGA display minimum resolution 1024x780 pixels, 65536 colors.

Input/output connections

Arcnet A and B; Cable from the camera to the PC

Stacker

The instrument can have none, one or two stackers. A stacker has place for 32 plates fitted into plate holders. The stacker is designed to take plates that fulfill the following requirements: 127-129 x 85-89 x 0.5-25.0 mm (LxWxH)

Measurement modes

Prompt fluorometry

Fluorescence polarization

Time-resolved fluorometry

LANCE

Luminometry

Photometry

Custom mode

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Specifications, continued

Barcode

Barcode length: max. 60 mm

Barcode height: min. 5 mm

Empty space at the ends of barcode label: min. 10 mm

Minimum bar width: min. 0.25 mm

Bar-space ratio: 1/3

Non-fluorescent label material

Code types:

• code39 (optional check digit)

• interleaved2/5 (optional check digit)

• codabar

• code128

Other barcodes may work, but should be tested individually.

Filters supplied

Filter type Description

DUG11 Schott Color Glass Filter 340 nm ±30 nm

405/10 Narrow Band Interference Filter 405 nm ±5 nm

450/20 Narrow Band Interference Filter 450 nm ±10 nm

480/20 Narrow Band Interference Filter 480 nm ±10 nm

540/25 Narrow Band Interference Filter 540 nm ±12.5 nm

618/8 Narrow Band Interference Filter 618 nm ±4 nm

671/8 Narrow Band Interference Filter 671 nm ±4 nm

525/20 Narrow Band Interference Filter 525 nm ±10 nm

598/25 Narrow Band Interference Filter 598 nm ±12.5 nm

613/55 Narrow Band Interference Filter 613 nm ±27.5 nm

564/250 Absorbance Emission Filter 564 nm ±125 nm

Clear Infrared Damping Filter 400 nm – 750 nm

Black – A black opaque plate to act as a shutter

Polarizers for FP

Optional filters Description

573/6 Narrow Band Interference Filter 573 nm ±3 nm

547/8 Narrow Band Interference Filter 547 nm ±4 nm

600/10 Narrow Band Interference Filter 600 nm ±5 nm

Filter systems for different measurement modes

Label system excitation emission

Prompt fluorescence:

UMB, AMC DUG11 450/20

FITC 480/20 540/25

Luminescence: Infrared damping filter

Absorbance: 405/10 (in excitation filter wheel)

480/20 (in excitation filter wheel)

564/250 (in emission filter wheel)

Black (in emission filter wheel)

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Copyright ©2011, PerkinElmer, Inc. All rights reserved. PerkinElmer® is a registered trademark of PerkinElmer, Inc. All other trademarks are the property of their respective owners. 009980_01 Nov 2011 Printed in USA

PerkinElmer, Inc. 940 Winter Street Waltham, MA 02451 USA P: (800) 762-4000 or (+1) 203-925-4602www.perkinelmer.com

Specifications, continued

Performance

Fluorescence in white 384-well plate with 50 μL sample volume

Fluorescein detection limit 5 fmol/well with FITC dichroic mirror, black plate

Umbelliferone detection limit 250 fmol/well

Rhodamine detection limit 25 fmol/well

Fluorescence in white 1536-well plate with 7.5 μL sample volume

Umbelliferone detection limit 100 fmol/well

TR-Fluorescence, DELFIA®, in white 384-well plate with 50 μL sample volume, with 30 s imaging time

Eu detection limit 50 amol/well with 2X binning

TR-Fluorescence, DELFIA, in white 1536-well plate with 7.5 μL sample volume, with 30 s imaging time

Eu detection limit 500 amol/well

Luminescence in white 384-well plate with 50 μL sample volume and with 1 min imaging time

Firefly Luciferase detection limit 5 fg/well with 4X binning

Luminescence in white 1536-well plate with 7.5 μL sample volume and with 1 min imaging time

Firefly Luciferase detection limit 25 fg/well with 2X binning

Absorbance in black, clear bottom 384-well plate with 50 μL sample volume

Photometric detection in the range of 0-1 absorbance units, 405 nm filter and Tartrazine samples.

Imaging wavelength 400 nm-800 nm

Spatial resolution 3 lp/mm

Dynamic range of the camera 16 bits/pixel

Uniformity of the plate <5% CV

Signal reproducibility CV <5%/1min/plate within 100 plates

Fluorescence polarization in black Costar® (Corning Inc.) 384-well plate with 40 μL sample volume. Samples 1 nM FITC and 1 nM BODIPY TMR

Imaging time: 30 s for FITC and 45 s for BODIPY

Intraplate standard deviation FITC 5 mP , BODIPY TMR 5 mP

Fluorescence polarization in black Greiner 1536-well plate with 5 μL sample volume

Samples 1 nM FITC and 1 nM BODIPY TMR

Imaging time: 60 s for FITC and 90 s for BODIPY

Intraplate standard deviation FITC 6 mP, BODIPY TMR 8 mP

Absorbance in black, clear bottom 1536-well plate with 7.5 μL sample volume