vitamin c
DESCRIPTION
Vitamin C. Vitamin C. Vitamin C also known as ascorbic acid or ascorbate . The human being is one of the few mammals unable to synthesize vitamin C. Other animals unable to synthesize vitamin C include primates, fruit bats, guinea pigs, and some birds. - PowerPoint PPT PresentationTRANSCRIPT
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Vitamin C
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Vitamin C • Vitamin C also known as ascorbic acid or ascorbate.• The human being is one of the few mammals unable
to synthesize vitamin C. • Other animals unable to synthesize vitamin C include
primates, fruit bats, guinea pigs, and some birds.• The inability to synthesize vitamin C results from the
lack of gulonolactone oxidase, the last enzyme in the vitamin C synthetic pathway.
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History of Vitamin C
• Vitamin C was isolated in 1928, and its structure was determined in 1933.
• The problems referred to as scurvy and associated with the lack of vitamin C had been quite prevalent for centuries. Some of the most notable stories are those of the British sailors who often died from scurvy on sea.
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Vitamin C structure
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Vitamin C • Vitamin C have a number of vitamiers that
have vitamin C activity in animals: • Ascorbic acid in biological system (low pH)• Oxidized form deyhroascorbic acid. • Ascorbate in neutral salutation (pH>5)• Ascorbate and ascorbic acid are both naturally
present in the body, since the forms interconvert according to pH.
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Sources • Fruits, vegetables and organ meats (liver, kidney). • The best food sources of vitamin C include asparagus,
papaya, oranges, cantaloupe, cauliflower, broccoli, green peppers, grapefruit, grapefruit, lemons, and strawberries.
• Citrus products are most commonly cited as significant sources of the vitamin.
• Supplements supply vitamin C typically as free ascorbic acid, calcium ascorbate, sodium ascorbate, and ascorbyl palmitate.
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Sensitivity of vitamin C
• Very significant losses occur as vegetables wilt, or when they are cut, as a result of the release of ascorbate oxidase from the plant tissue.
• Significant losses of the vitamin also occur in cooking, both through leaching into the cooking water and also atmospheric oxidation, which continues when foods are left to stand before serving.
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DIGESTION, ABSORPTION & TRANSPORT • Vitamin C does not require digestion prior to being
absorbed into intestinal cells.• There is active transport of the vitamin at the
intestinal mucosal brush border membrane. Both ascorbate and dehydroascorbate are absorbed across the buccal mucosa by carrier-mediated passive processes.
• Intestinal absorption of dehydroascorbate is carrier mediated, followed by reduction to ascorbate before transport across the membrane.
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DIGESTION, ABSORPTION & TRANSPORT • Prior to absorption, ascorbic may be oxidized
to dehydroascorbate, which may be absorbed by facilitated diffusion.
• Although dehydroascorbic acid is absorbed in higher rate than ascorbate, the amount of dehydroascorbic acid found in plasma and tissues under normal conditions is low, as cells rapidly reduce dehyroascorbic acid to ascorbate.
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DIGESTION, ABSORPTION & TRANSPORT • Simple diffusion (may occur in stomach and
small intestine) provides for vitamin C absorption with ingestion of higher amounts of the vitamin.
• Anion channels in some cells may mediate vitamin C diffusion faster than the transporters.
• From intestinal cells, ascorbate may diffuses through anion channels into extracellular fluid and enters plasma by way of capillaries.
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DIGESTION, ABSORPTION & TRANSPORT • Within intestinal cells: • Dehydroascorbic acid is rapidly reduced back to ascorbic
acid by enzyme dehydroascorbate reductase. • Glutathione (GSH), which is required for the reduction of
dehydroascorbate, is oxidized in process. Glutathione spares vitamin C and, in general, improves the antioxidant protection capacity of blood.
• Degree of vitamin C absorption decrease with increased vitamin intake.
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DIGESTION, ABSORPTION & TRANSPORT • Some 80–95% of dietary ascorbate is
absorbed at usual intakes (up to about 100 mg/day).
• The fractional absorption of larger amounts of the vitamin is lower, and unabsorbed ascorbate from very high doses is a substrate for intestinal bacterial metabolism, causing gastrointestinal discomfort and diarrhea.
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The redox cycling of ascorbate in chloroplast
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DIGESTION, ABSORPTION & TRANSPORT • Absorption of ascorbate may be diminished in
the presence of high intracellular glucose, which appears to interfere with ascorbate transporter.
• Unabsorbed vitamin C may be metabolized by intestinal flora.
• Ingesting large amounts of iron with vitamin C may result in oxidative destruction of vitamin C in digestive tract, yielding other products without vitamin C activity.
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DIGESTION, ABSORPTION & TRANSPORT In plasma: • Ascorbic acid is transported in the plasma primarily in
free form as an ascorbate anion. • Normal plasma level of vitamin C 0.4 – 1.7 mg/dL. In cells:• Uptake of ascorbate into body cells requires sodium and
a carrier and into some cells such as leukocytes, uptake is also energy dependent.
• Tissue concentrations of vitamin C usually exceed plasma concentrations.
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DIGESTION, ABSORPTION & TRANSPORT • Ascorbate and dehydroascorbate concentrations are much
greater in some tissues than others. The highest in adrenal and pituitary glands (~30-50mg/100 g of wet tissue).
• Intermediate in liver, spleen, heart, kidneys, lungs pancreas and leukocytes.
• Smaller amounts in muscles and red blood cells. • Maximal vitamin C pool is estimated at about 1.500mg. • Intakes of about 100-200mg vitamin C/d have been shown
to produce plasma concentrations of about 1.0mg/dL and to maximize body pool.
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Summary of absorption • Absorbed in the intestine by an energy-requiring,
sodium- dependent, carrier- mediated transport system.
• After absorption, ascorbic acid is then transported as a free acid in plasma into the cells, including leukocytes and red blood cells.
• In the tissues, Vitamin C serves as an electron donor for a number of enzymes.
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Function & mechanism of action• Vitamin C has very complex functional roles in the body as a
cofactor in around eight reactions:1. Collagen synthesis2. Carnitine synthesis3. Tyrosine synthesis and catabolism4. Neurotransmitter synthesis.5. Drug and steroid metabolism. 6. Maintain the iron and copper atoms in the metalloenzymes in
the reduced state. 7. In addition vitamin C acting role as reducing agent in enzymatic
reactions. 8. Ascorbate may also act as an antioxidant against oxidative stress.
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Collagen Synthesis• Most abundant protein found in the body.• Vitamin C is necessary for collagen synthesis.• Collagen is a structural protein found in skin, bones, tendons, and
cartilage. • All collagen (n~19) have a triple helical structure. • For the collagen molecule to aggregate into its triple-helix configuration
selected proline residues must be hydroxylated forming hydroxyproline. • Requires di-oxygenase enzymes, reduced iron (Fe+2), ascorbate. • Vitamin C role:• During hydroxylation, iron cofactor in the enzymes is oxidized,
(ferrous(2+) state ---- ferric (3+) state).• Ascorbate is needed to function as a reductant thereby reducing iron
back to its ferrous (2+) in prolyl and lysyl hydroxylases.
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Collagen Synthesis • Vitamin C may also influence mRNA levels
needed for collagen synthesis. • Although these reactions may seen simple,
normal development and maintenance of skin, tendons, cartilage, bone and dentine depend on an adequate supply of vitamin C.
• Also, important in wound healing and bleeding prevention from capillaries.
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Collagen Synthesis
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Carnitine synthesisCarnitine is a methylated from nitrogen containing compound made from lysine.• Sufficient carnitine is critical in fat metabolism, because it
is essential to transport long- chain fatty acids from cell cytoplasm into mitochondrial matrix where β-oxidation occurs.
Vitamin C role:• Required for 2 hydroxylation reaction in synthesis carnitine,
which functions as preferred reducing agent, specifically reducing Fe from ferric (Fe3+ ) back to ferrous state (Fe2+ ).
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Tyrosine synthesis & catabolism • Tyrosine synthesis• Hydroxylation of phenylalanine• Requires phenylalanine mono-oxygenase (hydroxylase), Fe+2.
O2. tetrahydrobiopterin, NADPH, vitamin C • Vitamin C regenerating tetrahydro-biopterin from
dihydrobiopterin.• Occurs in liver and kidney. • Tyrosine catabolism require ascorbate as reductant for
hydroxylases • Cu-dependent enzyme p-hydroxyphyenylpyruvate (dioxygenase)• Fe-dependent enzyme homogentisate dioxygenase.
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Neurotransmitter synthesis • Vitamin C maintains mineral cofactors for some of
the enzymes involved in synthesis of neurotransmitters in its reduced state.
Norepinephrine• It is generated from hydroxylation of dopamine side
chain.• This reaction catalyzed by dopamine monoxygenase
(contain 8 Cu atoms)-vitamin C- dependent reaction. • Found in nervous tissue and adrenal medull.
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Dopamine Hydroxylase
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Neurotransmitter synthesis • Serotonin • Hydroxylation of tryptophan (in brain)• Requires tryptophan mono-oxygenase (hydroxylase), O2
tetrahydrobiopterin, vitamin C. first step in serotonin synthesis. • Other Neurotransmitters and Hormones • keeping Cu in reduced for peptidylgycine α-amidating mono-
oxygenase.• Many of amidated peptides are active as hormones, such as
calcitonin, CCK and gastrin. • The enzyme found in pituitary , adrenal, thyroid glands and
brain.
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Summary• Vitamin C acts as an electron donor for 8 different
enzymes:• Three enzymes participate in collagen hydroxylation. • Two enzymes are necessary for synthesis of carnitine.• Three remaining enzymes have the following functions
in common, but have other functions as well: • Dopamine β-hydroxylase participates in biosynthesis of
norepinephrine from dopamine. • Enzyme adds amide groups to peptide hormones. • One modulates tyrosine metabolism.
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Microsomal metabolism
• A group of enzymes makes up a microsomal metabolizing system, mostly function in liver, to inactivate both endo-and exogenous substances.
• Endogenous: Include various hormones and steroids (cholesterol)
• Exogenous: xenobiotics (foreign chemicals) drugs, carcinogens, pesticides, pollutants, food additives
• The reactions to metabolize these substances usually involve hydroxylation's followed by other reactions to produce polar metabolites for excretion.
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Antioxidants activity • The reduction potential of ascorbate is such that it
readily donates electrons/hydrogen ions to regenerate other antioxidants, such as vitamin E, glutathione, and uric acid, and to reduce numerous reactive oxygen (ROS) and nitrogen species (RNS).
• Ascorbic acid interact with oxidants in the aqueous phase (blood or intracellular) before they initiate damage in nucleus, cell lipids.
• Ascorbate = thiols > bilirubin > uric acid > vitamin E
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Pro-oxidant activity
• Vitamin C can reduce transition metals, while itself becoming oxidized to semidehydroascorbate:
Ascorbate (AH2) +Fe3+ or Cu2-
Semidehydroascorbate radical (AH−) + Fe2+ or Cu1+
• These reduced metal ions can cause cell damage by generating ROS and free radicals.
Fe2+ or Cu1+ + H2O2 Fe3+ or Cu2- + H2O2 + OHFe2+ or Cu1+ + O2 Fe3+ or Cu2- + O2-
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Other functions • Vitamin C and colds: Vitamin C is thought to moderate colds by enhancing many immune cell (such as some leukocyte) functions while also destroying histamine, which causes many of a cold’s symptoms.
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Other functions• Vitamin C and cancer: • It is controversial issue due to: • Epidemiological studies suggest inverse relationship
between vitamin C and cancers of oral cavity, esophagus and stomach.
• Clinical studies: • Some shown that survival time in cancer time patients
may be prolonged, possible protective mechanisms• Ability to act as a reducing agent • Detoxify carcinogens.
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Other functions • CVD:• Many (but not all) epidemiological and prospective studies report
that increased fruit and vegetable intake, vitamin C intake, and plasma vitamin C concentration are associated with decreased risk of heart disease.
• Low vitamin C status also is related to increased blood total cholesterol concentrations, whereas high plasma vitamin C concentrations have been associated with lower blood pressure and with higher plasma high-density lipoprotein cholesterol concentrations, both of which are protective against heart disease.
• studies with supplements of vitamin C and other antioxidants have not reported beneficial effects.
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Other functions
• Cataracts: negative relation with vitamin c• Bone density: Positive relation with vitamin C
• Wound healing and connective tissue metabolism.
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INTERACTIONS WITH OTHER NUTRIENTS
• Vitamin C and iron • Increase intestinal absorption of non-heme iron • Reduce Fe3+ to Fe2- forms a soluble complex with
iron in alkaline pH of SI. • Effect on iron distribution in the body. • Vitamin C aids incorporation of iron into ferritin • Excessive iron in presence of vitamin C can
accelerate oxidative catabolism of vitamin C.
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Interactions with other nutrients • Vitamin C and copper• May increase absorption and excretion of heavy
metals
• Vitamin C intakes above 600 mg/d may interfere with copper metabolism.
• Vitamin C helps keep folate in its reduced and active form.
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Excretion
• Excretion reduced when intake is low• Urinary excretion • Body pool <1.500mg leads to only metabolites in
urine• Body pool > 1500mg leads to proportionately
more ascorbate in urine• Vitamin C may be excreted intact or oxidized by L-
ascorbate oxidase to oxalic acid, primarily in liver and some in kidney.
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Vitamin C RDA
• Adult male: 90mg• Adult female: 75 mg• Pregnancy: 100my• Lactating: 120mg• Smokers: extra 35 mg• UL: adult 2000 mg.
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Deficiency
• Scurvy• Scurvy leads to formation of brown spots on skin,
bleeding gums, small red skin discolorations caused by ruptured small blood vessels (petechiae), sublingual hemorrhages, easy bruising (ecchymoses and purpurae), impaired wound and fracture healing, joint pain (arthralgia), loose and decaying teeth, and hyperkeratosis of hair follicles, especially on the arms, legs, and buttocks.
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Toxicity
• Toxicity is rare.• Tolerable upper intake level of 2g vitamin C/d • The most common side effect with ingestion of
large amounts (2 g) of the vitamin is gastrointestinal problems characterized by abdominal pain and osmotic diarrhea.
• Other side effects include increased risk of kidney stones and iron toxicity for those with renal disease and disorders of iron metabolism.