vitamins and coenzymes - gbv.de
TRANSCRIPT
Methods in Enzymology
Volume 281
Vitamins and CoenzymesPart K
EDITED BY
Donald B. McCormickDEPARTMENT OF BIOCHEMISTR Y
EMORY UNIVERSITY
ATLANTA, GEORGI A
John W. SuttieDEPARTMENTS OF BIOCHEMISTRY AND NUTRITIONAL SCIENCE S
UNIVERSITY OF WISCONSIN-MADISO N
MADISON, WISCONSIN
Conrad WagnerDEPARTMENT OF VETERANS AFFAIRS MEDICAL CENTE R
AND DEPARTMENT OF BIOCHEMISTRY
VANDERBILT UNIVERSITY SCHOOL OF MEDICIN E
NASHVILLE, TENNESSEE
CONTRIBUTORS TO VOLUME 281 .
.
i x
PREFACE xii i
VOLUMES IN SERIES x v
Section I . Folic Acid
1 . Total Chemical Synthesis of Chirally Pure (6S)- STEVEN W . BAILEY AN DTetrahydrofolic Acid
JUNE E. AYLING
3
2 . Analysis of Folates Using Combined Affinity and PAMELA J . BAGLEY ANDIon-Pair Chromatography
JACOB SELHUB
1 6
3 . Molar Quantitation of Folates by Gas Chromatogra- CHERUPPOLIL R .phy-Mass Spectrometry
SANTHOSH-KUMAR AN DJ. FRED KOLHOUSE
2 6
4 . Microbiological Assay of Folates in 96-Well Micro- DONALD W . HORNE
3 8titer Plates
5 . Microbiological Assay for Serum, Plasma, and Red ANNE M. MOLLOY AN DCell Folate Using Cryopreserved, Microtiter JOHN M . SCOTT
43
Plate Metho d
6 . Determination of Tetrahydrobiopterin Biosynthetic ERNST R. WERNER ,
Activities by High-Performance Liquid Chroma- HELMUT WACHTER, AN Dtography with Fluorescence Detection
GABRIELE WERNER-FELMAYER 53
7 . Microtiter Plate Assay for Biopterin Using Cryopre- ROBERT J . LEEMING
6 1served Crithidia fasciculata
8 . Identification and Assay of Folate Catabolites in JOE MCPARTLIN ANDHuman Urine
JOHN M . SCOTT
70
9 . Enzymatic Determination of Folylpolyglutamate VERNE SCHIRCH
7 7
Pool s
10. Synthesis and Interconversion of Reduced Folyl- VERNE SCHIRCH
8 1polyglutamate s
11 . Chemical Synthesis of (6S)-5-Formyl-5,6,7,8-tetra- ANTHONY L . FITZHUGH ANDhydropteroylpoly-y-L-glutamates
RHONE K. AKEE
S 8
12 . Biotin Derivatives of Folate Compounds : Synthesis JIANGUO FAN AN Dand Utilization for Visualization and Affinity Pu- FRANK HUENNEKENS
9 7
rification of Folate Transport Proteins
13. Preparation of Stable Isotopically Labeled Folates CHRISTINE M . PFEIFFER ANDfor in Vivo Investigation of Folate Absorption JESSE F . GREGORY III
10 6and Metabolis m
14. Interconversion of 6- and 7-Substituted Tetrahy- SHELDON MILSTIEN
11 6dropterins via Enzyme-Generated 4a-Hydroxy-tetrahydropterin Intermediate s
15 . Enzymatic Synthesis of 6R-[U- I 'C]Tetrahydro- MASAAKI HosHIGA AN Dbiopterin from [U- I4C]GTP
KAZUYUKI HATAKEYAMA
123
16. Use of 10-Formyl-5,8-dideazafolate as Substrate for ROBERT J . Co0K
12 9Rat 10-Formyltetrahydrofolate Dehydrogenas e
17. Human Cytosolic Folylpoly-y-glutamate Synthase IAN ATKINSON ,TIMOTHY GARROW ,ALBERT BRENNER, AN DBARRY SHANE
134
18. Folylpolyglutamate Synthase from Higher Plants HELENA C . (MESON AN DEDWIN A. COSSINS
14 1
19. Purification of Folate-Dependent Enzymes from VERNE SCHIRCH
14 6Rabbit Liver
20. Human 5,10-Methenyltetrahydrofolate Synthetase JACQUES JOLIVET
162
21 . Mitochondrial NAD-Dependent Methylenetetra- ROBERT E . MACKENZIE
17 1hydrofolate Dehydrogenase-Methenyltetrahy-drofolate Cyclohydrolas e
22 . Monofunctional NAD-Dependent 5 .10-Methylene- DEAN R . APPLING ANDtetrahydrofolate Dehydrogenase from Saccharo- MARY G . WEST
178myces cerevisiae
23 . Methionine Synthase from Pig Liver
RUMA BANERJEE ,ZHIQIANG CHEN, ANDSUMEDHA GULATI
189
24. Purification and Assay of Cobalamin-Dependent JOSEPH T . JARRETT ,Methionine Synthase from Escherichia coli
CELIA W . GOULDING ,KERRY FLUHR ,SHA HUANG, ANDROWENA G . MATTHEWS
196
25 . Formyltetrahydrofolatc Hydrolase from Esche- HOWARD ZALKIN
21 4richia coli
26. Use of 13 C Nuclear Magnetic Resonance to Evaluate DEAN R . APPLING ,Metabolic Flux through Folate One-Carbon EVDOKIA KASTANOS ,Pools in Saccharomyces cerevisiae
LAURA B . PASTERNACK, AN DYAKOV Y . WOLDMAN
218
Section II . Vitamin B 12 and Cobalamins
27 . Use of Magnetic Field Effects to Study Coenzyme CHARLES B . GRISSOM AN D
B12-Dependent Reactions
ETTAYA NATARA .IAN
235
28. Analysis of Cobalamin and Cobalamin Analogs by DAVID P. SUNDIN AND
Gas Chromatography-Mass Spectrometry
ROBERT H . ALLEN
247
29. Expression of Functional Intrinsic Factor Using Re- MARILYN M. GORDON ,
combinant Baculovirus
GREG RUSSELL-JONES, AND
DAVID H. ALPERS
25 5
30 . Quantitative Methods for Measurement of Transco- SHELDON P . ROTHENBERG AN D
balamin II
EDWARD V . QUADROS
26 1
31 . Molecular Methods for Analysis and Expression of LIAN QIAN ,
Transcobalamin II
EDWARD V . QUADROS, AND
SHELDON P. ROTHENBERG
26 9
32 . Purification, Membrane Expression, and Interac- SANTANU BOSE AN D
tions of Transcobalamin II Receptor
BELLUR SEETHARAM
28 1
33 . Purification and Characterization of Aquacoba- FUMIO WATANABE AN D
lamin Reductase from Euglena gracilis
YOSHIHISA NAKANO
28 9
34 . Purification and Characterization of Aquacoba- FuMIo WATANABE AN D
lamin Reductases from Mammals
YOSHIHISA NAKANO
29 5
Section III . Herne
35. Continuous Coupled Assay for 5-Aminolevulinate PETER M .
Synthase
SHOOLINGIN-JORDAN ,
JEREMY E. LELEAN, AND
ADRIAN J . LLOYD
309
36. Dipyrromethane Cofactor Assembly of Porpho- PETER M .bilinogen Deaminase : Formation of Apoenzyme SHOOLINGIN-JORDAN ,
and Preparation of Holoenzyme
MARTIN J. WARREN, AND
SARAH J. AWAN
31 7
37 . Coupled Assay for Uroporphyrinogen III Synthase PETER M.
SHOOLINGIN-JORDAN AND
ROBERT LEADBEATER
327
38 . Expression and Purification of Mammalian HARRY A . DAILEY AN D
5-Aminolevulinate Synthase
TAMARA A . DAILEY
336
39. Expression, Purification, and Characteristics of TAMARA A . DAILEY AND
Mammalian Protoporphyrinogen Oxidase
HARRY A . DAILEY
340
40. Purification and Properties of Uroporphyrinogen ANDREW G . ROBERTS AN D
Decarboxylase from Human Erythrocytes
GEORGE H. ELDER
349
41 . Purification and Properties of Coproporphyrinogen TAKEO YOSHINAOA
355
III Oxidase from Bovine Liver
42 . Purification and Properties of Coproporphyrinogen PIERRE LABBE
367III Oxidase from Yeast
43 . Expression, Purification ; and Characterization of VERA M . SELLERS AND
Recombinant Mammalian Ferrochelatase
HARRY A . DAIEEY
37 8
Section IV. Miscellaneous Vitamins and Coenzymes
44 . Structural Characterization of Modified Folates in ROBERT H. WHIT! ..
39 1Archae a
Section V. Methodologies Broadly Applicable to Vitamin sand Coenzymes
45 . Biokinetic Analysis of Vitamin Absorption and Dis- JANOS ZI :MPLENI
40 5position in Humans
46. In Situ Kinetics : An Approach to Recommended MARK LEVINE ,
Intake of Vitamin C
STEVEN C. RUMSEY ,
YAOHUI WANG,
JAE PARK,
ORAN KWON, AND
NOBUYUKI AMANO
42 5
AUTHOR INDEX 43 9
SUBJECT INDEX 457