Vol. 261, No. 33 The Journal of November 25,1986

Biological Chemistry Copyright 0 1986 by the American Society of Biological Chemists, Inc., 428 East Preston St., Baltimore, MD 21202 U.S.A.

CONTENTS*

15345

15349

15353

15356

15360

15364

15368

COMMUNICATIONS

A mutated bovine prochymosin zymogen can be ac- tivated without proteolytic processing at low pH.

Michael T. M e C a ~ n and Diana Begley C u m m i ~ s

Domain structure of rat liver carbamoyl phosphate synthetase I.

Susan G. Powers-Lee and Karen Corina

Characterization of a regulatory region of the ste- roid 21-hydroxylase gene.

Keith L. Parker, Bernard P. Schimmer, David D. Chap- lin, and J . G. Seidman

Cyanide binding to bovine heart cytochrome c oxi- dase depleted of subunit I11 by treatment with lauryl maltoside.

Bruce C. Hill and Neal C. Robinson

Evidence for a second isoform of the catalytic sub- unit of CAMP-dependent protein kinase.

Michael D. Uhler, John C. Chrioia, and G. Stanley McKnight

Structure of the human apolipoprotein B gene.

Pierotti, Lynda Cuiati, Martha A. O m c h , Simon C. Wallis, Brian D. Btackhart, Erwin M. Ludwig! Vincenzo R.

Lyn Powell, Richard Pease, Timothy J. Knott, Mon-Li Chu, Robert W. Mahley, James Scott, Brian J. Mecarthy, and Beatrzz Levy- Wdson

Selective externalization of an ATP-binding protein structurally related to the clathrin-uncoating ATP- aselheat shock protein in vesicles containing ter- minal transferrin receptors during reticulocyte maturation.

Jonathan Q. Davis, Danielle Dansereau, Rose M. John- stone. and Vann Benn.ett

15372

15378

15385

15390

15396

Thiolidisulfide exchange between rabbit muscle phosphofructokinase and glutathione. Kinetics and thermodynamics of enzyme oxidation.

David Wayne Walters and Hiram F. Gilbert

Cooperative behavior in the thiol oxidation of rabbit muscle glycogen phosphorylase in cysteamineicys- tamine redox buffers.

Roseann E. Cappel and Hiram F. Gilbert

Thiol/disulfide redox equilibrium between glutathi- one and glycogen debranching enzyme (amylo-1,6- glucosidase/4-a-glucanotransferase) from rabbit muscle.

mons, Thomas E. Nelson, and Hiram F. Gilbert Roseann E. Cappel, James W. Bremer, Therese M. Tim-

Channeling of 3-hydroxy-4-trans-decenoyl coen- zyme A on the bifunctional @-oxidation enzyme from rat liver peroxisomes and on the large subunit of the fatty acid oxidation complex from Escherichia coli.

Song- Yu Yang, Dean Cuebas, and Horst Schulz

Identification of the binding site for plasma prekal-

A region from residues 185 to 224 of the kininogen likrein in human high molecular weight kininogen.

light chain retains full binding activity. Jonathan F. Tait and Kazuo Fujikawa

15402

15410

15416

15424

15430

15437

15442

15450

15455

15461

15467

15474

15480

15486

Covalent modification of the inhibitor-binding site(s) of Escherichia coli ADP-glucose synthetase. Isolation and structural characterization of 8-azido- AMP-incorporated peptides.

Stimulation of prostaglandin Ea synthesis in cloned osteoblastic cells of mouse (MC3T3-El) by epider- mal growth factor.

Kazushige Yokota, Masami Kusaka, Takeo Ohshima,

and Masayoshi Kumegawa Shozo Yamamoto, Noriyoshi Kurihara, Takuhiko Yoshino,

The isolation, characterization, and comparison of the membrane-associated and soluble folate-binding proteins from human KB cells.

and J . Fred Kolhouse Patrick C. Elwood, Madeleine A. Kane, Raul M. Portillo,

Protein kinase C activation by cis-fatty acid in the absence of Ca2+ and phospholipids.

berg Kentaro Murakumi, Shew Y . Chan, and Aryeh Routten-

Degradation of proteins microinjected into HeLa cells. The role of substrate flexibility.

Kevin V. Rote and Martin Rechsteiner

Purification and identification of the functional so- dium- and chloride-coupled y-aminobutyric acid transport gllycoprotein from rat brain.

Rodica Radian, Annie Bendahan, and Baruch I. Kanner

binding region and the link protein from the chon- N-terminal carbamylation of the hyaluronic acid-

drosarcoma proteoglycan aggregate. Jeff W. Stevens and Vincent C. Hascall

human tumor T cell line. Degradation of interleukin Receptor-mediated endocytosis of interleukin 2 in a

leukin receptors. 2 and evidence for the absence of recycling of inter-

Purification and molecular characterization of hu- man transcobalamin 11.

E. Pan, and Stanley Stein Edward V. Quadros, Sheldon P. Rothenberg, Yu-Ching

Role of cyclic GMP in atrial natriuretic factor stimulation of Na+,K+,Cl- cotransport in vascular smooth muscle cells.

Charles E. Larsen, Young Moo Lee, and Jack Preiss

Vkronique Duprez and Alice Dautry- Varsat

Martha E. O’Donnell and Nancy E. Owen

Role of ternary complexes, in which heparin binds both antithrombin and proteinase, in the accelera- tion of the reactions between antithrombin and thrombin or Factor Xa.

Bjork Ake Daniekson, Elke Raub, iilf Lindahl, and Zngemar

A sulfhydryl presumed essential is not required for catalysis by an aminoacyl-tRNA synthetase.

The crystallographically determined structures of atypical strained disulfides engineered into subtili- sm.

Albert T. Profy and Paul S c h i m ~ l

Bradley A. Katz and Anthony Kossiakoff

Enhanced activation of CAMP-dependent protein kinase by rapid synthesis and degradation of CAMP.

Margarita Lesser, Norman Fleischer, and Jmk Erlzch- man

The CONTENTS arranged by Subject Categories will be found immediately following these CONTENTS. Full Instructions to Authors will be found in (1986) J. Biol. Chem. 261, 1-11, and reprints may be obtained from the editorial office.

11

... 111

15491

15496

15505

15508

15513

15519

15524

15529

15538

15544

15550

15557

15565

15572

15577

15582

The involvement of carboxylate groups of putida- redoxin in the reaction with putidaredoxin reduc- tase.

Lois Geren, Jody Tuk, Patricia O'Brien, Francis Millett, and Julian A. Peterson

The hydrolysis of extracellular adenine nucleotides by cultured endothelial cells from pig aorta. Feed- forward inhibition of adenosine production at the cell surface.

Ellen L. Gordon, Jeremy D. Pearson, and Linda L. Slakey

Appendix. Simulation of extracellular nucleotide hydrolysis and determination of kinetic constants for the ectonucleotidases.

Linda L. Slakey, Karen Cosimini, James P. Earls, Clive Thomas, and Ellen L. Gordon

by insulin. Relationship with lipid fluidity. Fusion of negatively charged phospholipid vesicles

Ricardo N. Farias, Albert0 Ldpez Virials, and Roberto D. Morero

carboxypeptidase A. A 'lsCd and 31P NMR study. On the coordination of inhibitors to the metal ion of

Metabolism of platelet-activating factor by arachi- donic acid-depleted rat polymorphonuclear leuko- cytes.

Dexamethasone differentially affects the levels of calcitonin and calcitonin gene-related peptide mRNAs expressed in a human medullary thyroid carcinoma cell line.

Peter Getttns

C~akkodabylu S. Ramesha and Walter C. Pickett

Gilbert J . Cote and Robert F. Gage1

A purified cysteine conjugate @-lyase from rat kid- ney cytosol. Requirement for an a-keto acid or an amino acid oxidase for activity and identity with soluble glutamine transaminase K.

James L. Stevens, Joan D. Rabbins, and R. Andrew Byrd

The odorant-sensitive adenylate cyclase of olfactory

classes of odorants. receptor cells. Differential stimulation by distinct

Pamela B. Sklar, Robert R. H. Anholt, and Solomon H. Snyder

Overexpression of a novel anionic glutathione transferase in multidrug-resistant human breast cancer cells.

diar G. Katki, Charles E. Myers, and Kenne~h H. Cowan Gerald Batist, Ani1 Tulpule, Birandra K. Sinha, Aspan-

On the molecular mechanism of lactoperoxidase- catalyzed HzOs metabolism and irreversible enzyme inactivation.

Herbert Jenzer, Walk Jones, and Heinz Kohler

Oxygen exchange between Pi in the medium and water during ATP hydrolysis mediated by skinned fibers from rabbit skeletal muscle. Evidence for Pi binding to a force-generating state.

and David R. Trentham Martin R. Webb, Mark G. Hibberd, Yale E. Goldman,

Affinity labeling of an allosteric ADP site of gluta- mate dehydrogenase by 2-(4-bromo-2,3-dioxobu- tylthio~adenosine 5'-monoph~sphate.

Surendra P. Batra and Roberta F. Colman

R~onstitution of Saccharomyces cerevisiae phos- phatidylserine synthase into phospholipid vesicles. Modulation of activity by phospholipids.

Joyce M. Hromy and George M. Carman

Regulation of carbamoyl phosphate synthetase-as- partate transcarbamoylase-dihydroorotase gene expression in growing and arrested cells.

Warren S. L. Liaa, Renu Heller, Peter Green, and Gearge R. Stark

A new yeast mutation in the glucosylation steps of the asparagine-linked glycosylation pathway. For- mation of a novel asparagine-linked oligosaccharide containing two glucose residues.

K w t W . Runge and Phillips W. Robbins

15591

15596

15601

15607

15615

15619

15625

15632

15637

15644

15648

15652

15659

15662

15668

15673

15679

Cross-linking site in fibrinogen for az-plasmin in- hibitor.

Shigeru Kimura and Nobuo Aoki

Reconstitution of ATP-dependent calcium transport from streptococci.

and Barry P. Rosen Suresh V. Ambudkar, Anita R. Lynn, Peter C. Maloney,

Metabolism and analysis of cysteinyl leukotrienes in the monkey.

ber, Dietmar Witker, Dietrich K. Hammer, and Dietrich Claudio Denzlinger, Albrecht Guhlmann, Peter H. Scheu-

Keppler

Cloning and characterization of cDNA encoding 3- methylcholanthrene inducible rat mRNA for UDP- glucuronosyitr%nsferase.

Yoshiaki Fujii-Kuriyama, Shinya Watanabe, John E. Takashi Iyanagi, Mitsuru Haniu, Kazuhiro Sogawa,

Shively, and Koichi F. Anan

Detergent-assisted refolding of guanidinium chlo- ride-denatured rhodanese. The effect of lauryl mal- toside.

Shobha T a ~ a n and Paul Horowitz

bovine serum albumin by affinity labeling. Location of long chain fatty acid-binding sites of

Roberta G. Reed

The interrelationship of the soluble and membrane-

cells. associated folate-binding proteins in human KB

Madeleine A. Kane, Patrick C. Elwood, Raul M. Portilla, Asok C. Antany, and J. Fred Kolhouse

ATPase activities of wheat germ initiation factors 4A, 4B, and 4F.

Sandra R. L a x , Karen S. Browning, Diane M. Maia, and Joanne M. Ravel

Purification and characterization of a protein-ty- rosine kinase from bovine thymus,

Thomm F. Zioncheck, Marietta L. Harrison, and Robert L. Geahlen

Quantitativechanges in inositol 1,4,5-trisphosphate in chemoattractant-stimulated neutrophils.

Peter G. Bradford and Ronald P. Rubin

Purified protein kinase C phosphorylates microtu- bule-associated protein 2.

Saji, Hiroshi Ogawara, M i n a ~ Hoshi, Y o s h i h i ~ Miyata, Tetsu Akiyama, Eisuke Nishida, Junko Ishida, Noriko

and Hikoichi Sakai

Low concentrations of guanidinium chloride expose apolar surfaces and cause differential perturbation in catalytic intermediates of rhodanese.

Paul Horowitz and Nick L. Criscimagna

Demonstration of the amphiphilic character of hor- mone-sensitive lipase by temperature-induced phase separation in Triton X-114 and charge-shift electrophoresis.

Cecilia Holm, Gudrun Fredrikson, and Per Belfrage

Apolipoprotein B mediates the capacity of low den-

by particulates. sity lipoprotein to suppress neutrophil stimulation

Robert Terkeltaub, Jody Martin, Linda K. Curtiss, and Mark H. Ginsberg

Purification and properties of the DNA invertase Gin encoded by bacteriophage Mu.

Gabriele Mertens, Heidemarie Fuss, and Regine Kah- mann

Purification and properties of the Escherichia coli host factor required for inversion of the G segment in bacteriophage Mu.

Christian Koch and Regine Kahmann

teolysis by VS and characterization of two func- Substructure of human von Willebrand factor. Pro-

tional domains.

Harold P. Erickson, and Patrick A. McKee Larry J . Fretto, Walter E. Fowler, Darrell R. McCaslin,

iv

15690

15696

15703

15709

15714

15719

15725

15734

15740

15746

15755

15761

Structure of an antifreeze polypeptide precursor from the sea raven, Hemitriptents umericunus.

Nancy F. Ng, Khiet-Yen Trinh, and Choy L. Hew

tion of a rat alveolar macrophage lectin. The ligand binding specificity and tissue localiza-

Robert S. Haltiwanger and Robert L. Hill Nucleotide sequence and transcriptional initiation site of two Pseudomonw aeruginosa pilin genes.

Kit Johnson, Michael L. Parker, and Stephen Lory

Complete overlap of caffeine- and K+ depolariza- tion-sensitive intracellular calcium storage site in cultured rat arterial smooth muscle cells,

Sei Kobayashi, Hideo Kanaide, and Motoomi Nakamura Apolipoprotein A-IV. A determinant for binding and uptake of high density lipoproteins by rat hepato- cytes.

Antonio M. Gotto, Jr. Elena Duorin, Nancy L . Gorder, Douglas M. Benson, and

Hemopexin is a developmentally regulated, acute- phase plasma protein in the chicken.

entina Goldfarb, Sylvia A. Metcalfe, and Ursula Muller- Gerd Grieninger, T. Jake Liang, Gerard Beuuing, Val-

Eberhard

ster ovary cells. Sulfation of glycosaminoglycans Sulfate transport-deficient mutants of Chinese ham-

dependent on cysteine.

H. Taylor, and Julre L. Wernke Jeffrey D. Esko, Ada Elguuish, Tom Prasthofer, William

Carbachol-induced protein phosphorylation changes in bovine tracheal smooth muscle.

Susanna Park and Howard Rasmussen Transcription and st-tran~riptional regulation of avian HSP7O gene expression.

Sunandita S. Banerji, Lee Berg, and Richard I. Morimoto A monoclonal antibody interfering with binding and response of the acetylcholine receptor.

Alfred Maelicke Gregor Fels, Rita Pliimer- Wilk, Michael Schreiber, and

Gangliosides modulate sodium transport in cultured toad kidney epithelia.

Sarah Spiegel, Joseph S. Handler, and Peter H. Fishman Purification and structure of 3-methyladenine- DNA glycosylase I of Escherichia coli.

moto, Shun-ichiro Kawabata, Sadaaki Iwanaga, and Mut- Kunihiko Sakumi, Yusaku Nakabeppu, Yohko Yama-

SUQ Sekiguchi

15767

15772

15778

15783

15787

15794

15800

15805

15813

ix

Purification and characterization of a novel glycan- phosphatidylinositol-specific phospholipase C from !Prypanosoma brucei.

Judith A. Fox, Michael Duszenko, Michael A. J. Fergu- son, Martin G. Low, and George A. M. Cross

erichiu coli related to the repair of alkylated DNA. Structure and expression of the alkB gene of Esch-

H i d e m a Kondo, Yusaku Nakabeppu, Hrroko Kataoka,

guchi Satoru Kuhara, Shun-ichiro Kawabata, and Mutsuo Seki-

Covalent coupling of a resact analogue to guanylate cyclase.

Garbers Hiromi Shimomura, Lawrence J. Dangott, and David L.

The nucleotide and partial amino acid sequence of toxic shock syndrome toxin- 1.

John S. K o r ~ l u m , Richard P. Nouick, and Patrick M. Debra A. Blomster-Hautamaa, Barry N. Kreiswirth,

Schlieuert

Induction, identification, and cell-free translation of mRNAs coding for peroxisomal proteins in Can- dida tropicalis.

~ e ~ - D e ~ s a , and Paul B. Lazarow Yukio Fujiki, Richard A. Rachubinski, Alejandro Zen-

NAD(P)H:menadione oxidoreductase. Novel purifi- cation of enzyme, cDNA and complete amino acid sequence, and gene regulation.

John A. Robertson, Hao-Chia Chen, and Daniel W. Ne- bert

Translational regulation of the synthesis of a major heat shock protein in HeLa cells.

Arrigo de Benedetti and Corrado Baglioni

Exchange of H1 histone depends on aggregation of chromatin, not simply on ionic strength.

Yong-jiu J in and R. David Cole

ADDITIONS AND CORRECTIONS

Purification of a three-subunit ubiquinol-cyto- chrome c oxidoreductase complex from Paracoccus denjtrificana. Vol. 261 (1986) 12282-12289.

X m h a n g Yang and Bernard L. Trumpower

Cumulative author index. Numbers 1 through 33.

GENERAL INFORMATION FOR 1986

Submit all manuscripts in triplicate to

9650 Rockville Pike Editor, The Journal of Biological Chemistry

Bethesda, MD 20814, U.S.A.

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Practices,” or for permission to reproduce any part of a previously published article should be directed to the Journal Editorial Office in Bet.hesda, teIephone,301-5~0-7150.

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Vol. 261, No. 33 The Journal of Biological Chemistry November 25,1986

CONTENTS Arrangec

CARBOHYDRATES, LIPIDS, AND OTHER NATURAL PRODUCTS

15442 N-terminal carbamylation of the hyaluronic acid- binding region and the link protein from the chon- drosarcoma proteoglycan aggregate.

Jeff W. Stevens and Vincent C. Hascall

15519 Metabolism of platelet-~tivating factor by arachi- donic acid-depleted rat polymorphonuclear leuko- cytes.

Chakkodabylu S. Ramesha and Walter C. Pickptt

15582 A new yeast mutation in the glucosylation steps of the asparagine-linked glycosylation pathway. For- mation of a novel asparagine-linked oligosaccharide containing two glucose residues.

Kurt W. Runge and Phillips W. Robbins

15662 Apolipoprotein B mediates the capacity of low den- sity lipoprotein to suppress neutrophil stimulation by particulates.

Mark H. Ginsberg Robert Terkeltaub, Jody Martin, Linda K, Curtiss, and

15696 The ligand binding specificity and tissue localiza- tion of a rat alveolar macrophage lectin.

Robert S. Haltiwanger and Robert L. Hili

CELL BIOLOGY AND METABOLISM 015368 Selective externalization of an ATP-binding protein

structurally related to the clathrin-uncoating ATP- aselheat shock protein in vesicles containing ter- minal transferrin receptors during reticulocyte maturation.

Jonathan Q. Davis, Danielk Dansereau, Rose M. John- stone, and Vann Bennett

15430 Degradation of proteins microinjected into HeLa cells. The role of substrate flexibility.

Kevin V. Rote and Martin Rechsteiner

15450 Receptor-mediated endocytosis of interleukin 2 in a human tumor T cell line. Degradation of interleukin 2 and evidence for the absence of recycling of inter- leukin receptors.

Veroniqw Duprez and Alice Dautry- Varsat

15461 Role of cyclic GMP in atrial natriuretic factor stimulation of Na+,K+,Cl- cotransport in vascular smooth muscle cells.

Martha E. O'Donnell and Nancy E. Owen

15496 The hydrolysis of extracellular adenine nucleotides by cultured endothelial cells from pig aorta. Feed- forward inhibition of adenosine production at the cell surface.

Ellen L. Gordon, Jeremy D. Pearson, and Linda L. Slakey

15505 Appendix. Simulation of extracellular nucleotide hydrolysis and determination of kinetic constants for the ectonucleotidases.

Linda L. Slakey, Karen Cosimini, James P. Earls, Clive Thomas, and Ellen L. Gordon

15538 The odorant-sensitive adenylate cyclase of olfactory receptor cells. Differential stimulation by distinct classes of odorants.

Snyder Pamela B. Sklar, Robert R. H. Anholt, and Solomon H.

3 by Subject Categories

15601 ~ e t a ~ l i s m and analysis of cysteinyl leukotrienes in the monkey.

Claudw Denzlinger, Albrecht Guhlmann, Peter H. Scheu- ber, Dietmar Wilkpr, D ie~r~ch K. Hammer, and Dietrich Keppler

15625 The interrelationship of the soluble and membrane- associated folate-binding proteins in human KB cells.

Asok C. Antony, and J . Fred Kolhouse Madeleine A. Kane, Patrick C. Elwood, Raul M. Portillo,

15644 Quantitativechanges in inositol 1,4,5-trisphosphate in chemoattractant-stimulated neutrophils.

15648 Purified protein kinase C phosphorylates microtu- bule-associated protein 2.

Tetsu Akiyama, Eisuke Nishida, Junko Ishida, Noriko Saji, Hiroshi Ogawara, Minako Hoshi, Yoshihiko Miyata, and H i ~ i c h i Sakai

15709 Complete overlap of caffeine- and K+ depolariza- tion-sensitive intracellular calcium storage site in cultured rat arterial smooth muscle cells.

Sei Kobayashi, Hide0 Kanaide, and Motoomi Nakamura

15714 Apolipoprotein A-IV. A determinant for binding and uptake of high density lipoproteins by rat hepato- cytes.

Elena Dvorin, Nancy L. Gorder, Dougtas M. Benson, and Antonio M. Gotto, Jr.

15725 Sulfate transport-deficient mutantsof Chinese ham- ster ovary cells. Sulfation of glycosam~noglycans dependent on cysteine.

Jeffrey D. Esko, Ada Elgavish, Tom Prasthofer, William H. Taylor, and Julie L. Weinke

Peter G. B r ~ f o r d and Ronald P. Rubin

15734 Carbachol-induced protein phosphorylation changes in bovine tracheal smooth muscle.

Susanna Park and Howard Rasmussen

15755 Gangliosides modulate sodium transport in cultured

Sarah Spiegel, Joseph S. Handler, and Peter H. Fishman toad kidney epithelia.

16778 Covalent coupling of a resact analogue to guanylate

Hiromi Shimomuru, Laurence J. Dangott, and David L. cyclase.

Garbers

ENZYMOLOGY 015345 A mutated bovine prochymosin zymogen can be ac-

tivated without proteolytic processing at low pH. Michael T. McCaman and Diana Begley Cummings

15372 ThiolJdisulfid~ exchange between rabbit muscle phosphofructokinase and glutathione. Kinetics and thermodyn~ics of enzyme oxidation.

David Wayne Walters and Hiram F- Gilbert

15378 Cooperative behavior in the thiol oxidation of rabbit muscle glycogen phosphorylase in cysteaminelcys- tamine redox buffers.

Roseann E. Cappel and Hiram F. Gilbert

15385 ThiolJdisulfide redox equilibrium between glutathi- one and glycogen debranching enzyme (amylo- 1,6- glucosidase/4-a-glucanotransferase) from rabbit muscle.

mons, Thomas E. Nelson, and Hiram F. Gilbert Roseann E. Cappel, James W. Rremer, Therese M. Tim-

I5544 Overexpression of a novel anionic glutathione 15390 Channeling of 3-hydroxy-~-~~a~-decenoyl coen- transferase in multidrug-resistant human breast

zyme A on the bifunctional &oxidation enzyme from rat liver peroxisomes and on the large subunit of

cancer cells. the fatty acid oxidation complex from Escherichia

diar G. Katki, Charles E. Myers, and ken net^ H. Cowan Gerald Bathst, Ani1 Tulpule, Birandra K. Sinha, Anspan- coli.

Song- Yu Yang, Dean Cuebas, and Horst Schulz

Indicates a Communication

V

vi

15410

15424

15467

15474

15486

15491

15529

15550

15557

15565

15637

15659

15767

-15356

15437

Stimulation of prostaglandin Ez synthesis in cloned osteoblastic cells of mouse (MC3T3-El) by epider- mal growth factor.

Kamshige Yokota, Masami Kusaka, Takeo Ohshima, Shozo Yamamoto, Noriyoshi Kurihara, Takahiko Yoshino, and Masayoshi Kumegawa

Protein kinase C activation by cis-fatty acid in the absence of Ca2' and phospholipids.

herg Kentaro Murakami, Shew Y . Chan, and Aryeh Routten-

Role of ternary complexes, in which heparin binds both antithrombin and proteinase, in the accelera- tion of the reactions between antithrombin and thrombin or Factor Xa.

Bjork Ake Daniekson, Elke Raub, Ulf Lindahl, and Ingemar

A sulfhydryl presumed essential is not required for catalysis by an aminoacyl-tRNA synthetase.

kinase by rapid synthesis and degradation of CAMP. Enhanced activation of CAMP-dependent protein

Margarita Leiser, Norman Fleischer, and Jack Erlich- man

The involvement of carboxylate groups of putida- redoxin in the reaction with putidaredoxin reduc- tase.

and Julian A. Peterson Lois Geren, Jody Tuls, Patricia O'Brien, Francis Millett,

A purified cysteine conjugate #?-lyase from rat kid- ney cytosol. Requirement for an 0-keto acid or an amino acid oxidase for activity and identity with soluble glutamine transaminase K.

James L. Stevens, Joan D. Robbins, and R. Andrew Byrd

Albert T. Profy and Paul Schimmel

On the molecular mechanism of lactoperoxidase- catalyzed HzOa metabolism and irreversible enzyme inactivation.

Herbert Jenzer, Walis Jones, and Heinz Kohler

Oxygen exchange between Pi in the medium and water during ATP hydrolysis mediated by skinned fibers from rabbit skeletal muscle. Evidence €or Pi binding to a force-generating state.

and David R. Trentham Martin R. Webb, Mark G. Hibberd, Yale E. Goldman,

Affinity labeling of an allosteric ADP site of gluta- mate dehydrogenase by 2-(4-bromo-2,3-dioxobu- tylthiotadenosine 5'-monophosphate.

Purification and characterization of a protein-ty- rosine kinase from bovine thymus.

L. Geahlen Thomas F. Zioncheck, Marietta L. Harrison, and Robert

Demonstration of the amphiphilic character of hor-

phase separation in Triton X-114 and charge-shift mone-sensitive lipase by temperature-induced

electrophoresis.

Puri€ication and characterization of a novel glycan- phosphatidylinositol-specific phospholipase C from Trypanosoma brucei.

son, Martin G. Low, and George A. M. Cross Judith A. Fox, Michael Duszenko, Micha.el A. J. Fergu-

Surendra P. Batra and Roberta F. Colman

Cecilia Holm, Gudrun Fredrikson, and Per Belfrage

MEMBRANES AND BIOENERGETICS Cyanide binding to bovine heart cytochrome c oxi- dase depleted of subunit I11 by treatment with lauryl maltoside.

Bruce C. Hill and Neal C. Robinson

Purification and identification of the functional so- dium- and chloride-coupled y-aminobutyric acid transport glycoprotein from rat brain.

Rodica Radian, Annie Bendahan, and Baruch. I. Kanner

15508

15596

15813

I

015353

*15360

*15364

15524

15572

15577

15607

15632

15668

15673

15'703

15719

15740

Fusion of negatively charged phospholipid vesicles by insulin. Relationship with lipid fluidity.

Morero Ricardo N. Farias, Albert0 Ldpez Vir%&, and Roberto D.

Reconstitution of ATP-dependent calcium transport from streptococci.

and Barry P. Rosen Suresh V. Ambudkar, Anita R. Lynn, Peter C. Maloney,

Purification of a three-subunit ubiquinol-cyto- chrome c oxidoreductase complex from Paracoccus denitrificans. Vol. 261 (1986) 12282-12289.

Xiuohang Yang and Bernard L. Trumpower

WCLEIC ACIDS, PROTEIN SYNTHESIS, AND MOLECULAR GENETICS

Characterization of a regulatory region of the ste- roid 21-hydroxylase gene.

lin, and J. G. Seidman Keith L. Parker, Bernard P. Sch~mmer, David D. Chap-

Evidence for a second isoform of the catalytic sub- unit of CAMP-dependent protein kinase.

McKnight Michae~ D. Uhler, John C. Chrivia, and G. Stanley

Structure of the human apolipoprotein B gene. Brian D. Blackhart, Erwin M. Ludwig, Vincenzo R.

Pierotti, Lynda Caiati, Mar~ha A. Onasch, Simon C. Wallis, Lyn Powell, Richard Pease, Timothy{. Knott, Mon-Li Chu, Robert W. Mahley, James Scott, Brtan J. McCarths, and Beatriz Levy- Wilson Dexamethasone differentially affects the levels of calcitonin and calcitonin gene-related peptide mRNAs expressed in a human medullary thyroid carcinoma cell line.

Gilbert J . Cote and Robert F. Gage1

Reconstitution of Saccharomyces cerevisiae phos- phatidylserine synthase into phospholipid vesicles. Modulation of activity by phospholipids.

Joyce M. Hromy and George M. Carman

partate transcarbamoylase-dihydroorotase gene Regulation of carbamoyl phosphate synthetase-as-

expression in growing and arrested cells.

R. Stark Warren S. L. Liao, Renu Heller, Peter Green, and George

Cloning and characterization of cDNA encoding 3- methylcholanthrene inducible rat mRNA for UDP- glucuronosyltransferase.

Yoshiaki Fujii-Kuriyama, Shinya Watanabe, John E. Takashi Iyanagi, Mitsuru Haniu, Kazuhiro Sogawa,

Shively, and Koichi F. Anan ATPase activities of wheat germ initiation factors 4A, 4B, and 4F.

Joanne M. Ravel Sandra R. Lax, Karen S. Browning, Diane M. Maia, and

Purification and properties of the DNA invertase Gin encoded by bacteriophage MU.

Gabriele Mertens, Hecdemarce Fuss, and Regine Kah- mann Purification and properties of the Escherichia coli host factor required for inversion of the G segment in bacteriophage Mu.

Nucleotide sequence and transcriptional initiation site of two Pseudomonas aeruginosa pilin genes.

Kit Johnson, Michael L. Parker, and Stephen Lory

Hemopexin is a developmentally regulated, acute- phase plasma protein in the chicken.

Gerd Grieninger, T. Jake Liang, Gerard Beuving, Val- entina Goldfarb, Sylvia A. Metcalfe, and Ursula Muller- ~ b e r ~ r d Transcription and post-transcriptional regulation of avian HSP70 gene expression.

Sunandita S. Banerji, Lee Berg, and Richard I. Morimoto

C h r i s t ~ n Koch and Regine Kahmann

Indicates a Communication

vii

The isolation, characterization, and comparison of the membrane-associated and soluble folate-binding proteins from human KB cells.

and J . Fred Kolhouse Patrick C. Elwood, Madeleine A. Kane, Raul M. PortiUo,

Purification and molecular characterization of hu- man transcobalamin 11.

E. Pan, and Stanley Stein Edward V. Quadros, Sheldon P. Rothenberg, Yu-Ching

The crystallographically determined structures of

sin. atypical strained disulfides engineered into subtili-

On the coordination of inhibitors to the metal ion of carboxy eptidase A. A 'I3Cd and 31P NMR study.

Cross-linking site in fibrinogen for aa-plasmin in- hibitor.

Deter ent assisted refoldin of uanidinium cblo- ride-&natured rhodanese. #he egect of lauryl mal- toside.

Location of lon chain fatt acid-binding sites of bovine serum algumin by afgnity labeling.

Low concentrations of guanidinium chloride expose apolar surfaces and cause differential perturbation in catalytic intermediates of rhodanese.

Paul Horowltz and Nick L. Crmrmagna Substructure of human von Willebrand factor. Pro- teolysis by V8 and characterization of two func- tional domains.

Bradley A . Katz and Anthony Kossiakoff

Peter &ttins

Shigeru Kimura and Nobuo Aoki

Shobha Tandon and Paul Horowitz

Roberta G. Reed

15761 Purification and structure of 3-methyladenine- DNA glycosylase I of Escherichia coli.

Kunihiko Sakumi, Yusaku Nakabeppu, Yohko Yama- moto, Shun-ichiro Kaurabata, Sadaaki Iwanaga, and Mut- suo Sekiguchi

15772 Structure and expression of the dkB gene of Esch- erichia coli related to the repair of alkylated DNA.

Satoru Kuhara, Shun-ichiro Kawabata, and Mutsuo Seki- Hidemdsa Kondo, Yusaku Nakabeppu, Hiroko Kataoka,

guchi

15787 Induction, identification, and cell-free translation of mRNAs coding for peroxisomal proteins in Cun- dida tropicalis.

Yukio Fujiki, Richard A. Rachubinski, Alejandro Zen- tella-Dehesa, and Paul B. Lazarow

15794 NAD(P)H:menadione oxidoreductase. Novel purifi- cation of enzyme, cDNA and complete amino acid sequence, and gene regulation.

bert John A. Robertson, Hao-Chia Chen, and Daniel W. Ne-

15800 Translational regulation of the synthesis of a major heat shock protein in HeLa cells.

Arrigo de Benedetti and Corrado Baglioni

15805 Exchange of H I histone depends on aggregation of chromatin, not simply on ionic strength.

Yong-jiu Jin and R. David Cob

PROTEIN CHEMISTRY AND STRUCTURE 015349 Domain structure of rat liver carbamoyl phosphate

synthetase I. Susan G. Powers-Lee and Karen Corina

15396 Identification of the binding site for plasma prekal- likrein in human high molecular weight kininogen. A region from residues 185 to 224 of the kininogen light chain retains full binding activity.

Jonathan F. Tait and Kazuo Fujikawa

15402 Covalent modification of the inhibitor-binding site(s) of Escherichia coli ADP-glucose synthetase. Isolation and structural characterization of S-azido- AMP-incorporated peptides.

Charles E. Larsen, Young Moo Lee, and Jack Preiss

15416

15455

15480

15513

15591

15615

15619

15652

15679

15690

15746

15783

Lar J Fretto, Walter E. Fowler, Darrell R. McCaslin, ~ ~~ ~

Haro l~P . 'Er i c~on , and Patrick A. McKee Structure of an antifreeze polypeptide precursor from the sea raven, Hemitripterus americanus.

Nancy F. Ng, Khiet-Yen Trinh, and Choy L. Hew A monoclonal antibody interfering with binding and response of the acetylcholine receptor.

Gre or Fels, Rita Pliimer- Wilk, Michael Schreiber, and AlfrecfMaelccke The nucleotide and partial amino acid sequence of toxic shock syndrome toxin- 1.

Debra A. Blomster-Hautamaa, Barry N. Kreiswirth, John, S. Kornblum, Richard P. Novick, and Patrick M. Schllevert

Indicates a Communication