Voltammetric detection of LSD using a chemically modified carbon paste electrode with Schiff base

complex

Marcelo Firmino de Oliveira

Universidade de São PauloFaculdade de Filosofia Ciencias e Letras de Ribeirão Preto

Departamento de Química

Class A Class B Class C

FTIR  CE

 Spot tests

MS GC fluorescence

H-RMN  TLC

 Immunological assay

Raman HPLC Melting point

  Optical microscopy UV-Vis

  Farmaceutical identifiers

 

   

Drug analysis by american CSIs and Brazilian Federal Police

A+A

A+B

A+C

B+B+B

B+B+C

PS: no electrochemical methods cited

Cell, electrodes and expected results in voltammetry

Cocaine

(SWV: LOD of 1nmol/L)

Example of electrochemical analysis in forensic chemistry:

V. Pavlova et al. / Analytica Chimica Acta 512 (2004) 49–56

LSD

1938 – Dr. Albert Hofmann

Lysergic acid + diethylamided-LSD d-iso-LSD

l-iso-LSDl-LSDActuation: CNS

hallucinations; euphoria;panic; confusion;psychotic episodes.

Schiff Bases: 1864 – Hugo Schiff Amine + Aldehyde/Ketone

applicability : antimicrobial Fungicide Herbicide Anticancer

Complexation with metallic centers: UO2

2+

Chemical modifierChemical modifier

OBJECTIVES

Apply the complex [UO2(Ac-ophen)]·H2O as carbon paste electrode modifier;

Voltammetric detection and quantification of LSD.

40%20%

40%

CV

SWV DPV KCl 0.1M

ExperimentalExperimental

Standard addition – SWV

Frequency: 10 Hz

Amplitude: 800 mV

ResultsResults

Standard addition - DPV

Amplitude: 800mV

pulse time: 40ms

Voltammetric

technique

R2 SD

(μA)

m

(μA L μmol-1)

LOD

(μmol L-1)

LOQ

(μmol L-1)

SWV 0.992 1.79x10-2 1.75x10-1 0.31 1.02

DPV 0.984 2.63x10-2 1.52x10-1 0.52 1.73

CONCLUSIONS

Thank you

Good stability for the proposed CM-CPE in water.

A new transductor for LSD analysis;

Detection and quantification of LSD μmol L-1 range;