vwr collection pcr enzymes
DESCRIPTION
The aim of this catalogue is to provide you with a handy overview of our full VWR Collection PCR Enzymes available in Denmark, Finland, Norway, Portugal and Spain.TRANSCRIPT
VWR COLLECTION PCR ENZYMES
Manufactured by Ampliqon for VWR International Europe
Denmark, Finland, Norway, Portugal and Spain
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TEXT Birgitte Vedelstorp Andersen
LAYOUT Anne Mette Jensen [di’zain]
IMPRINT VWR Norway and Ampliqon A/S, Skovlunde, Denmark 2010
PRINTER’S FROM Grafisk as, Kolding, Denmark
This catalogue is for distribution in Denmark, Finland, Norway, Portugal and Spain
The aim of this catalogue is to provideyou with a handy overview of our fullVWR Collection PCR Enzymes availablein Denmark, Finland, Norway, Portugaland Spain.
FLEXIBILITY AND TAILORED SOLUTIONSSince 2008 VWR International has offered our PCR enzymes to Europeancustomers.
We work closely together withAmpliqon, the Danish manufacturer of our VWR Collection PCR enzymes, to offer a wide range of high qualityenzymes suitable for all DNA amplifica-tion purposes in use in your laboratory.
The cooperation between VWR andAmpliqon allows us to tailor custom-made solutions for individual customersat competitive prices. The same highquality and affordable prices are thebasis of our comprehensive standardPCR enzyme product line.
SAMPLESWe acknowledge that samples are thebest way to put our quality to the test.Therefore, we maintain an extensivesampling programme, which permityou to try our enzymes in your specificamplification set-up.
SUPPORT The VWR customer service has readyaccess to the Ampliqon support team,which facilitates convenient processingof requests and technical questionswithin 24 hours.
The VWR Collection PCR enzymes israpidly becoming a still more recog-nized life science brand in Europe andwe hope that this catalogue showsthat we are proud to be your supplierof high quality reagents.
Kind regardsVWR InternationalLife Science Europe
DEAR CUSTOMER
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TAQ 4
Taq DNA Polymerase 4
Glycerol free 4
Mg free 4
Tween free 4
Taq DNA Polymerase Master Mix 6
Taq RedPOL DNA Polymerase 6
Taq DNA Polymerase Master Mix RED 7
HIGH FIDELITY 8
AccuPOL DNA Polymerase 8
Tween free 8
HOT START 10
TRUeHOT DNA Polymerase 10
TRUeHOT DNA Polymerase Master Mix 12
TRUeHOT DNA Polymerase Master Mix BLUE 13
Multiplex TRUeHOT DNA Polymerase Master Mix 14
NUCLEOTIDES 15
dNTP Mix 15
dNTP Set 15
VWR PRACTICAL INFORMATION 17
ABOUT AMPLIQON 18
CONTENTS
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TAQ DNA POLYMERASE
VWR taq DNA Polymerase is suitablefor routine PCR applications, whichrequire high yield and reliable DNAamplification.
FEATURES� A thermostable recombinant
DNA polymerase� Enzyme isolated from
Thermus Aquaticus� Molecular weight of approximately
95 kDa� Leaves an A’ overhang
DESCRIPTION� VWR Taq DNA Polymerase exhibits
both a 5’ 3’ DNA polymerase and a 5’ 3’ exonuclease activity.
� VWR Taq DNA Polymerase has no proofreading activity
� Alternative VWR taq combinations also available:Glycerol freeMg freeTween freeExtra mM MgCl2
TAQ DNA POLYMERASE
PRODUCT NUMBER
733-1300 250 units
733-1301 500 units
733-1302 1000 units
733-1819 2500 units
733-1820 5000 units
733-1303 10000 units
Glycerol free
733-1817 1000 units
Mg free and Extra Buffer
733-1304 500 units
733-1305 1000 units
TAQ DNA POLYMERASE
PRODUCT NUMBER
Mg free and Key Buffer
733-1311 500 units
733-1312 1000 units
733-1313 2500 units
733-2009 10000 units
Tween free
733-1307 1000 units
733-1823 10000 units
20 mM MgCl2733-1826 1000 units
TAQ
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TAQ
ENZYME DILUTION Comparison between VWR Taq DNA Polymerase and four competitors. Amplification of a 160 bp fragment of the human phenylalanine hydroxylase (PAH) gene. Each amplification has been conducted according to supplier’sinstructions.
Enzyme dilution
COMPARISON BETWEEN VWR TAQ DNA POLYMERASE AND COMPETITORS
Enzyme dilution
VWR
1 x 2 x 4 x 6 x 1 x 2 x 4 x 6 x
Competitor I
VWR Competitor F
VWR Competitor B
VWR Competitor A
1 x 2 x 4 x 6 x 1 x 2 x 4 x 6 x
1 x 2 x 4 x 6 x 1 x 2 x 4 x 6 x
1 x 2 x 4 x 6 x 1 x 2 x 4 x 6 x
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TAQ DNA POLYMERASEMASTER MIX
VWR Taq DNA Polymerase Master Mix is an alternative to Taq DNAPolymerase. Taq DNA PolymeraseMaster Mix offers reduced set-up time and eliminates the risk of con-tamination of reaction components.Fewer handling steps lead to betterreproducibility, which makes Taq DNAPolymerase Master Mix suitable forstandard tests.
FEATURES� Premixed solution� Minimal optimization� Fewer pipetting steps
DESCRIPTIONTaq DNA Polymerase Master Mix is aready to use 1.1 x or 2.0 x reactionmix. Taq DNA Polymerase Master Mix is composed of VWR Taq DNAPolymerase, NH4+ buffer system,dNTPs and magnesium chloride.
TAQ DNA POLYMERASE MASTER MIX
PRODUCT NUMBER
1.1 x and 1.5 mM MgCl2733-2540 500 reactions
733-1314 2500 reactions
1.1 x and 2.0 mM MgCl2733-2541 500 reactions
733-1315 2500 reactions
2.0 x and 1.5 mM MgCl2733-2542 500 reactions
733-1316 2500 reactions
2.0 x and 2.0 mM MgCl2733-2543 500 reactions
733-1317 2500 reactions
REDPOL TAQ DNAPOLYMERASE
VWR RedPOL Taq DNA Polymerase isan alternative to standard Taq DNAPolymerase. The red dye provides con-venient identification of enzyme tubeand confirms complete mixing.
FEATURES� The inert dye has no effect on down-
stream processes
DESCRIPTIONVWR RedPOL Taq DNA Polymerase isadded directly to the reaction mix.
REDPOL TAQ DNA POLYMERASE
PRODUCT NUMBER
733-1323 2500 units
733-1834 10000 units
TAQ
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TAQ DNA POLYMERASEMASTER MIX RED
VWR Taq DNA Polymerase Master Mix RED is an alternative to Taq DNAPolymerase Master Mix with the sameadvantages.The inert red dye and stabilizer are extra features, whichallow for direct loading onto your agarose gel for analysis.
FEATURES� No need for separate loading dye� Taq DNA Polymerase Master Mix RED
is ready-made for electrophoresis and visualization
� Fewer handling steps
DESCRIPTIONTaq DNA Polymerase Master Mix RED isa ready to use 1.1 x or 2.0 x reactionmix. Taq DNA Polymerase Master MixRED is available in two final MgCl2concentrations: 1.5 mM and 2.0 mM.
Taq Polymerase Master Mix RED iscomposed of VWR Taq DNAPolymerase, NH4+ buffer system,dNTPs and magnesium chloride.
TAQ DNA POLYMERASE MASTER MIX RED
PRODUCT NUMBER
1.1 x and 1.5 mM MgCl2733-2544 500 reactions
733-1318 2500 reactions
1.1 x and 2.0 mM MgCl2
733-2545 500 reactions
733- 1319 2500 reactions
2.0 x and 1.5 mM MgCl2733-2546 500 reactions
733-1320 2500 reactions
2.0 x and 2.0 mM MgCl2733-2547 500 reactions
733-1321 2500 reactions
TAQ
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ACCUPOL DNAPOLYMERASE
VWR AccuPOL DNA Polymerase hasproofreading abilities and is targeted atPCR amplications, which require highfidelity or blunt endings.
FEATURES� AccuPOL DNA Polymerase is a
thermostable enzyme� Processes <3 kb
DESCRIPTIONAccuPOL DNA Polymerase exhibits both5’ 3’ DNA polymerase activity and 3’ 5’ proofreading exonuclease acti-vity. AccuPOL kits include an extra 25mM MgCl2 concentration if higherMgCl2 is required for specific reactions.
VWR AccuPOL DNA Polymerase alsocomes in two alternative versions:Mg free and Tween free
ACCUPOL DNA POLYMERASE
PRODUCT NUMBER
733-1324 250 units
733-1325 500 units
733-1326 1000 units
Mg and Tween free
733-1328 250 units
733-1329 1000 units
HIGH FIDELITY
* The error rate equals mutation frequency per base per duplication.
FIDELITY COMPARISONS OF THERMOSTABLE DNA POLYMERASES USING THE LACIOZ ASSAY
ENZYME ACCUPOL PFU TAQ
Error rate* (fidelity) 1.1 x 10-6 1.3 x 10-6 18.0 x 10-6
Elongation rate (bases/sec) 30 25 61
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TRUEHOT DNAPOLYMERASE
VWR TRUeHOT DNA Polymerase hasbeen designed to avoid spurious ampli-fication and to diminish the formationof non-specific priming events duringset-up. TRUeHOT DNA Polymeraseallows for low target copy numbersand for multiplex PCR in a single reaction tube.
FEATURES� Reaction set-up at room temperature
INCREASED� Sensitivity� Specificity� Product yield
� Ideal for real time PCR applications (real time protocol included)
� Suitable for multiplex applications
DESCRIPTIONTRUeHOT Hot Start DNA Polymerase isa modified form of VWR Taq DNAPolymerase, which is activated by heattreatment.
TRUeHOT DNA Polymerase is providedin an inactive state with no polymeraseactivity at room temperature due to a temperature dependent peptide inhibition of the DNA polymerase activity. Thus, during set up and thefirst ramp of thermal cycling, the enzyme is not active and misprimedprimers are not extended.
TRUeHOT DNA Polymerase is activatedby a 95° C preincubation step.
HOT START
TRUEHOT DNA POLYMERASE
PRODUCT NUMBER
733-1843 500 units
733-1844 2500 units
733- 1845 10000 units
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HOT START
VWR Competitor Q Competitor P Competitor I Competitor F Competitor FS
Chemical Antibody Antibody Chemical Genetic modified hot based hot modified hot modified hot modified hot
start Taq start Taq start Taq start Taq start Taq
Comparison with and without hot start activation: VWR TRUeHOT DNA Polymerase and competitors. Amplification ofa 210 bp fragment of the phenylalanine hydroxylase (PAH) gene conducted according to supplier’s instructions.
Comparison with and without hot start activation: TRUeHOT Hot Start DNA Polymerase and competitor Q.Amplification has been conducted according to supplier’s instructions.
AMPLIFICATION WITH AND WITHOUT HOT START
Fluorescence (dRn)4.7
3.7
2.7
1.7
0.7
-0.31 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 Cycles
10 units TRUeHOT, 100 ng DNA, hot start
10 units TRUeHOT, 100 ng DNA, no hot start
5 units TRUeHOT, 100 ng DNA, hot start
5 units TRUeHOT, 100 ng DNA, no hot start
10 units competitor Q, 100 ng DNA, hot start
10 units competitor Q, 100 ng DNA, no hot start
5 units competitor Q, 100 ng DNA, hot start
5 units competitor Q, 100 ng DNA, no hot start
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TRUEHOT DNA POLYMERASE MASTER MIX
VWR TRUeHOT DNA Polymerase 2 xMaster Mix is a ready to use mix thatoffers easy reaction assembly at roomtemperature prior to hot start PCR. Just add template and primers.TRUeHOT DNA Polymerase 2 x MasterMix is an alternative to TRUeHOT DNAPolymerase and is applicable for primerextensions and other amplification purposes.
FEATURES� Minimal optimization� Convenient reaction set-up� Fewer pipetting steps� Consistent test results
DESCRIPTIONVWR TRUeHOT DNA Polymerase, NH4+buffer system, dNTPs and magnesiumchloride are present in TRUeHOT DNAPolymerase 2 x Master Mix.
TRUEHOT DNA POLYMERASE 2 X MASTER MIX
PRODUCT NUMBER
733-2550 500 reactions
733-1847 2500 reactions
HOT START
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TRUEHOT DNAPOLYMERASE MASTERMIX BLUE
The blue loading dye facilitates directgel loading and eliminates the need forseparate loading dye for electrophore-sis and visualization after PCR.TRUeHOT DNA Polymerase 2 x MasterMix BLUE is an alternative to TRUeHOTDNA Polymerase 2 x Master Mix withextra time saving advantages.
FEATURES� Inactive at room temperature� Premixed 2 x solution
DESCRIPTIONVWR TRUeHOT DNA Polymerase 2 x Master Mix BLUE consists ofTRUeHOT DNA Polymerase, dNTPs, a blue loading dye and magnesiumchloride.
TRUEHOT DNA POLYMERASE 2 X MASTER MIX BLUE
PRODUCT NUMBER
733-2010 500 reactions
733-1849 2500 reactions
HOT START
Comparison with and without 15-minute hot start activation. TRUeHOT DNA Polymerase (lanes 1 and 2). TRUeHOTDNA Polymerase 2 x Master Mix BLUE (lanes 3 and 4). A DNA marker (lane 5).
TRUEHOT COMPARISON
Lane 1 2 3 4 5
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HOT START
MULTIPLEX TRUEHOTDNA POLYMERASEMASTER MIX
Multiplex PCR is a method that enablesamplification of two or more ampliconssimultaneously in a single reactiontube. Multiplex TRUeHOT DNAPolymerase 2 x Master Mix is suitablefor genotyping applications and variousareas of DNA testing in research, forensic and diagnostic laboratories.
FEATURES� Minimal optimization� Reduced set-up time� Minimizes contamination of
component stock� Fewer handling steps� Preincubation at 95° C
INCREASED� Reproducibility� Specificity
DESCRIPTIONVWR Multiplex TRUeHOT DNAPolymerase Master Mix containsTRUeHOT DNA Polymerase. The poly-merase is provided in an inactive statewith no polymerase activity at roomtemperature due to a temperaturedependent peptide inhibition of theDNA polymerase activity. This preventsthe formation of primer-dimers andmisprimed products during reactionset-up.
The multiplex PCR buffer contains abalanced composition of salt and addi-tives to ensure comparable efficienciesfor annealing and extension of all pri-mers in the reaction and facilitates theamplification of multiplex PCR pro-ducts.
Multiplex TRUeHOT DNA PolymeraseMaster Mix is provided with a PCRadditive betaine solution that facilitatesamplification of difficult templates bymodifying the melting behavior of theDNA.
MULTIPLEX TRUEHOT DNA POLYMERASE MASTER MIX
PRODUCT NUMBER
733-1851 250 reactions
733-1852 2500 reactions
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NUCLEOTIDES
DNTP
FEATURES� PCR amplification components� For DNA polymerization, DNA
labelling and sequencing processes� High purity: > 98 % by HPLC� pH 7.5
DNTP MIX
Ready to use solutionMix of dATP, dCTP, dGTP and dTTP
DNTP MIX
PRODUCT NUMBER
25 mM of each dA, dC, dG and dT
733-1854 (2 x 1000 µl) 2000 µl
733-1363 (2 x 500 µl) 1000 µl
DNTP SET
Set of dATP, DCTP, dGTP and dTTP
DNTP SET
PRODUCT NUMBER
100 mM dA, dC, dG and dT
733-1364 (4 x 250 µl) 1 set
733-1855 (16 x 250 µl) 1 set
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PRACTICAL INFORMATION
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DENMARKVWR - Bie & BerntsenTransformervej 82730 HerlevTel.: 43 86 87 88Fax: 43 86 87 90E-mail: [email protected]://dk.vwr.com
FINLANDVWR International OyValimotie 900380 HelsinkiTel.: 09 80 45 51Fax: 09 80 45 52 00E-mail: [email protected]://fi.vwr.com
NORWAYVWR International ASHaavard Martinsens vei 300978 OsloTel.: 02290Fax: 815 00 940 E-mail: [email protected]://no.vwr.com
FOR ENQUIRY, PURCHASE AND SUPPORT KINDLYCONTACT YOUR LOCAL VWR DISTRIBUTION PARTNER
PORTUGALVWR International - Material deLaboratório, LdaEdifício NeoparkAv. Tomás Ribeiro, 43- 3 D2790-221 CarnaxideTel.: 21 3600 770Fax: 21 3600 798/9E-mail: [email protected]://pt.vwr.com
SPAINVWR International Eurolab S.L.C/ Tecnologia 5-17Llinars Park08450 - LLinars del VallèsBarcelonaTel.: 902 222 897Fax: 902 430 657E-mail: [email protected]://es.vwr.com
For latest news, special offers anddetailed info on VWR please go to:www.vwr.com
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ABOUT AMPLIQON
Ampliqon A/S is a Danish manufacturerof PCR enzymes and bioreagents.
Ampliqon was established as a smallscale PCR enzyme company in 2002. In2009 we took over a comprehensivebioreagent product line.
Today, Ampliqon is a recognized lifescience reagents supplier with focus onquality, small batch production, flexibili-ty and good service.
Our modern production facility is situa-ted on the outskirts of Copenhagenwith optimum laboratories and a highlyskilled laboratory staff.
We value our well-run cooperationwith VWR International on VWRCollection PCR enzymes in Europe and custom-made Ampliqon labelledbioreagents in Denmark.
PCR
The Ampliqon science team has beenan active party in the field of PCR fromthe first development of advanced PCRtechnologies to the PCR method pre-vailing today.
Our expert knowledge of the impor-tant enzyme purification process andDNA amplification backs our day today production procedures and labora-tory team.
We proudly manufacture reliable, highquality PCR enzymes and willinglyshare our product and technical know-ledge with the VWR product specialists.
CUSTOM-MADE BIOREAGENTS
Ampliqon manufactures more than1000 custom-made bioreagents forDanish VWR customers. Our full VWRcustomer protocol archive holds about10000 recipes.
The proprietary customer protocols aretailored to fit the individual needs ofeach client and are shipped directlyfrom Ampliqon to hospitals, biotechindustries, research and educationalinstitutions all over Denmark on a dailybasis.
We appreciate that VWR Denmarkselected Ampliqon as their sole biore-agent supplier.
VWR International is exclusive distribu-tor of Ampliqon bioreagents.
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ABOUT AMPLIQON
WWW.VWR.COM