wastewater grown green microalgae – fatty acids ... · scenedesmus dimorphus coelastrella sp....
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Wastewater grown green microalgae – fatty acids characterization and quantification
The extraction method and pre-treatment effectSandra Lage
@SandraMFLage
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Microalgae cultivation
Scenedesmus dimorphus
Coelastrella sp.
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Gravimetric analysis
How to extract lipids from microalgae?
Gas Chromatography
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How to extract lipids from microalgae?
Gas Chromatography
Purificationstep
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Thin-layer chromatography (TLC) Solid phase extraction (SPE)
Transmethylation
How to purify total lipid extracts for Gas chromatography (GC) analysis?
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Make it Simpler, Make it Better Extraction, Purification and Transmethylation of Fatty acids in a Single-Step Method
or
Transmethylation
or
Nothing
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FAMEs characterization The extraction method effect
Scenedesmus dimorphus Coelastrella sp.
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FAMEs quantification The extraction method effect
Scenedesmus dimorphus Coelastrella sp.
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Fresh boiled in Isopropanol
Frozen at -20°C
Oven-Dry at 105°C
Freeze-Dry at -45°C
Pre-Treatment of Green microalgaeDoes it affect the quantity and/or quality of the lipids?
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FAMEs characterization The pre-treatment effect
Scenedesmus dimorphus Coelastrella sp.
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FAMEs quantification The pre-treatment effect
Scenedesmus dimorphus Coelastrella sp.
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http://biofuelregion.se/en/projekt/transalgae/infosheet/
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Conclusion• TLC and SPE purifications, coupled with single-step crude lipids extraction and
transmethylation, produced accurate, precise and reproducible results of FAME yields and
composition.
• SPE is preferable due to lower labor intensity.
• Removal of a purification step strongly affected the method reproducibility.
• DT had a distinct FAME profile in comparison with the other methods.
• Pretreatment did not have a strong impact in FAME characterization.
• Freeze-dried microalgae produce the lowest FAME yields.