lab · web viewinfective site: blood (lymphocytes "wbcs", and erythrocytes "rbcs...
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Lab.13 Sporozoa
Family : plasmodiidaeGenus : Plasmodium ( causing the malaria )
P.malariae , P.vivax , P.falciparum , P.ovale ( in human )
P.gallinaceum , P.relictum ( in birds )
Definitive Hosts: Rodents, Rabbits, Birds, and human
Final host : Mosquites ( Anopheles , Culex )
Infective Site: Blood ( RBCs ) , Liver
Infective Stage: sporozoite ( Sporozoites are motile and they move by gliding,
centrally located nucleus )
*Ring form : the cytoplasmic ring in the RBCs . The parasite may be first visible as
a crescent – shaped mass at the outer edge of the red blood cell .
*Developing trophozoites : the parasite takes on more of an irregularly shaped
ameboid appearance .
*Schizont : characterized by the presence of multiple chromatin bodies , The
merozoites (non-motile) surrounded by cytoplasmic material occupy the majority of
the RBCs.
*Gametocytes : A male gametocyte growing to give many flagellated ( micro -
gametocytes ) , whereas the female gametocyte differentiates to macrogametocytes .
*Diagnosis : - clinical signs are associated with anemia and fever .
-Thick and thin blood smears staind with giemsa stain and Serology diagnosis .
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Family : Theileriidae
Genus : TheileriaT. parva : The cause of bovine Theileriosis and East coast fever.
T. annulata : Also the cause of bovine and T. equi Causing Equine prioplasmosis
Definitive Hosts: Cattle, Sheep/Goats.
Final host : Ticks
Infective Stage: sporozoite.
Infective Site: Blood (lymphocytes "WBCs", and erythrocytes "RBCs ")
* There are two pictures for schizont (Kochs blue bodies) macroschizonts and
microschizonts that produce the micromerozoites in the red blood cell.
Diagnosis :
1 -Clinical Signs (Diarrhea, loses weight rapidly, anemia and fever .
2 -Blood smears with Giemsa stain and In Ramonowsky-stained smears, schizonts
appear as bluish bodies, approximately 8-12 microns in diameter with multiple
reddish nuclei .Intraerythrocytic piroplasms are similar to small babesial parasites.
They are approximately 0.5-2.5 microns and pleomorphic, but most often appear rod-
shaped, round or oval .
3 -Serological test (PCR and ELISA).
Schizont in RBC Merozoite
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Family : Babesiidae
Genus : BabesiaBabesia is pear , roundish and irregular shape in RBCs, have two form ( large and
small ) that causes a hemolytic disease known as Babesiosis & Texas cattle fever .
can be transmitted from human to human either through the tick vector or through
blood transfusions .
Definitive Hosts: Cattle, Sheep/Goats , Horse and dog.
Babesia microti infected the human , life cycle involves two hosts ( tick and mouse )
Final host : Hard Ticks ( Ixodidae )
Infective Stage: sporozoite.
Infective Site: Blood .
Diagnosis : 1 -clinical symptoms: such as high fever, anemia, and hemoglobinuria, in the
infected hosts.
2 - Thick and Thin blood sample testing : the cell contain vacuoles, and the lack of
pigment production. Trophozoites morph into merozoites that appear in a tetrad
formation( Maltese-cross form) .
3 -Serodiagnosis indirect fluorescent antibody (IFA) test .
Babesia canis
Trophozoites
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