Cell Biology-Cell structure and cell functions: Within cells there is an intricate network of organelles that all have unique functions. These organelles allow the cell to function properly. Arranged below according to location ( nucleus , cytoplasm , and surface ) is a description of common organelles.

One or more per cell Spherical shape

Denser than surrounding cytoplasm

Chromosomes- Usually in the form of chromatin- Contains genetic information- Composed of DNA- Thicken for cellular division- Set number per species (i.e. 23 pairs for human)

Nuclear membrane- Surrounds nucleus- Composed of two layers- Numerous openings for nuclear traffic

Nucleolus- Spherical shape- Visible when cell is not dividing- Contains RNA for protein manufacture

Collective term for cytosol and organelles contained within Colloidal suspension Cytosol mainly composed of water with free-floating molecules

Viscosity constantly changes

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Centrioles- Paired cylindrical organelles near nucleus- Composed of nine tubes, each with three tubules- Involved in cellular division- Lie at right angles to each other

Chloroplasts- A plastid usually found in plant cells- Contain green chlorophyll where photosynthesis takes place

Cytoskeleton- Composed of microtubules- Supports cell and provides shape- Aids movement of materials in and out of cells

Endoplasmic reticulum- Tubular network fused to nuclear membrane- Goes through cytoplasm onto cell membrane- Stores, separates, and serves as cell's transport system- Smooth type: lacks ribosomes- Rough type (pictured): ribosomes embedded in surface

Golgi apparatus- Protein 'packaging plant'- A membrane structure found near nucleus- Composed of numerous layers forming a sac

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Lysosome- Digestive 'plant' for proteins, lipids, and carbohydrates- Transports undigested material to cell membrane for removal- Vary in shape depending on process being carried out- Cell breaks down if lysosome explodes

Mitochondria- Second largest organelle with unique genetic structure- Double-layered outer membrane with inner folds called cristae- Energy-producing chemical reactions take place on cristae- Controls level of water and other materials in cell- Recycles and decomposes proteins, fats, and carbohydrates, and forms urea

Ribosomes- Each cell contains thousands- Miniature 'protein factories'- Composes 25% of cell's mass- Stationary type: embedded in rough endoplasmic reticulum- Mobile type: injects proteins directly into cytoplasm

Vacuoles- Membrane-bound sacs for storage, digestion, and waste removal- Contains water solution- Contractile vacuoles for water removal (in unicellular organisms)

Cell wall- Most commonly found in plant cells- Controls turgity- Extracellular structure surrounding plasma membrane- Primary cell wall: extremely elastic- Secondary cell wall: forms around primary cell wall after growth is complete

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Plasma membrane- Outer membrane of cell that controls cellular traffic- Contains proteins (left, gray) that span through the membrane and allow passage of materials- Proteins are surrounded by a phospholipid bi-layer.

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The Cell Cycle | Despite differences between prokaryotes and eukaryotes, there are several common features in their cell division processes. Replication of the DNA must occur. Segregation of the "original" and its "replica" follow. Cytokinesis ends the cell division process. Whether the cell was eukaryotic or prokaryotic, these basic events must occur.

Cytokinesis is the process where one cell splits off from its sister cell. It usually occurs after cell division. The Cell Cycle is the sequence of growth, DNA replication, growth and cell division that all cells go through. Beginning after cytokinesis, the daughter cells are quite small and low on ATP. They acquire ATP and increase in size during the G1 phase of Interphase. Most cells are observed in Interphase, the longest part of the cell cycle. After acquiring sufficient size and ATP, the cells then undergo DNA Synthesis (replication of the original DNA molecules, making identical copies, one "new molecule" eventually destined for each new cell) which occurs during the S phase. Since the formation of new DNA is an energy draining process, the cell undergoes a second growth and energy acquisition stage, the G2 phase. The energy acquired during G2 is used in cell division (in this case mitosis).

The cell cycle. Image from Purves et al., Life: The Science of Biology, 4th Edition, by Sinauer Associates (www.sinauer.com) and WH Freeman (www.whfreeman.com), used with permission.

Regulation of the cell cycle is accomplished in several ways. Some cells divide rapidly (beans, for example take 19 hours for the complete cycle; red blood cells must divide at a rate of 2.5 million per second). Others, such as nerve cells, lose their capability to divide once they reach maturity. Some cells, such as liver cells, retain but do not normally utilize their capacity for division. Liver cells will divide if part of the liver is removed. The division continues until the liver reaches its former size.

Cancer cells are those which undergo a series of rapid divisions such that the daughter cells divide before they have reached "functional maturity". Environmental factors such as changes in temperature and pH, and declining nutrient levels lead to declining cell division rates. When cells stop dividing, they stop usually at a point late in the G1 phase, the R point (for restriction).

Prokaryotic Cell Division |

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Prokaryotes are much simpler in their organization than are eukaryotes. There are a great many more organelles in eukaryotes, also more chromosomes. The usual method of prokaryote cell division is termed binary fission. The prokaryotic chromosome is a single DNA molecule that first replicates, then attaches each copy to a different part of the cell membrane. When the cell begins to pull apart, the replicate and original chromosomes are separated. Following cell splitting (cytokinesis), there are then two cells of identical genetic composition (except for the rare chance of a spontaneous mutation).

The prokaryote chromosome is much easier to manipulate than the eukaryotic one. We thus know much more about the location of genes and their control in prokaryotes.

One consequence of this asexual method of reproduction is that all organisms in a colony are genetic equals. When treating a bacterial disease, a drug that kills one bacteria (of a specific type) will also kill all other members of that clone (colony) it comes in contact with.

Eukaryotic Cell Division | Due to their increased numbers of chromosomes, organelles and complexity, eukaryote cell division is more complicated, although the same processes of replication, segregation, and cytokinesis still occur.

Mitosis | Mitosis is the process of forming (generally) identical daughter cells by replicating and dividing the original chromosomes, in effect making a cellular xerox. Commonly the two processes of cell division are confused. Mitosis deals only with the segregation of the chromosomes and organelles into daughter cells.

Eukaryotic chromosomes occur in the cell in greater numbers than prokaryotic chromosomes. The condensed replicated chromosomes have several points of interest. The kinetochore is the point where microtubules of the spindle apparatus attach. Replicated chromosomes consist of two molecules of DNA (along with their associated histone proteins) known as chromatids. The area where both chromatids are in contact with each other is known as the centromere the kinetochores are on the outer sides of the centromere. Remember that chromosomes are condensed chromatin (DNA plus histone proteins).

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Structure of a eukaryotic chromosome. Image from Purves et al., Life: The Science of Biology, 4th Edition, by Sinauer Associates (www.sinauer.com) and WH Freeman (www.whfreeman.com), used with permission.

During mitosis replicated chromosomes are positioned near the middle of the cytoplasm and then segregated so that each daughter cell receives a copy of the original DNA (if you start with 46 in the parent cell, you should end up with 46 chromosomes in each daughter cell). To do this cells utilize microtubules (referred to as the spindle apparatus) to "pull" chromosomes into each "cell". The microtubules have the 9+2 arrangement discussed earlier. Animal cells (except for a group of worms known as nematodes) have a centriole. Plants and most other eukaryotic organisms lack centrioles. Prokaryotes, of course, lack spindles and centrioles; the cell membrane assumes this function when it pulls the by-then replicated chromosomes apart during binary fission. Cells that contain centrioles also have a series of smaller microtubules, the aster, that extend from the centrioles to the cell membrane. The aster is thought to serve as a brace for the functioning of the spindle fibers.

Structure and main features of a spindle apparatus. Image from Purves et al., Life: The Science of Biology, 4th Edition, by Sinauer Associates (www.sinauer.com) and WH Freeman (www.whfreeman.com), used with permission.

The phases of mitosis are sometimes difficult to separate. Remember that the process is a dynamic one, not the static process displayed of necessity in a textbook.

Meiosis | Sexual reproduction occurs only in eukaryotes. During the formation of gametes, the number of chromosomes is reduced by half, and returned to the full amount when the two gametes fuse during fertilization.

Ploidy | Haploid and diploid are terms referring to the number of sets of chromosomes in a cell. Gregor Mendel determined his peas had two sets of alleles, one from each parent. Diploid organisms are those with two (di) sets. Human beings (except for their gametes), most animals and many plants are diploid. We abbreviate diploid as 2n. Ploidy is a term referring to the number of sets of chromosomes. Haploid organisms/cells have only one set of chromosomes, abbreviated as n. Organisms with more than two sets of chromosomes are termed polyploid. Chromosomes that carry the same genes are termed homologous chromosomes. The alleles on homologous chromosomes may

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differ, as in the case of heterozygous individuals. Organisms (normally) receive one set of homologous chromosomes from each parent.

Meiosis is a special type of nuclear division which segregates one copy of each homologous chromosome into each new "gamete". Mitosis maintains the cell's original ploidy level (for example, one diploid 2n cell producing two diploid 2n cells; one haploid n cell producing two haploid n cells; etc.). Meiosis, on the other hand, reduces the number of sets of chromosomes by half, so that when gametic recombination (fertilization) occurs the ploidy of the parents will be reestablished.

Most cells in the human body are produced by mitosis. These are the somatic (or vegetative) line cells. Cells that become gametes are referred to as germ line cells. The vast majority of cell divisions in the human body are mitotic, with meiosis being restricted to the gonads.

Life Cycles | Life cycles are a diagrammatic representation of the events in the organism's development and reproduction. When interpreting life cycles, pay close attention to the ploidy level of particular parts of the cycle and where in the life cycle meiosis occurs. For example, animal life cycles have a dominant diploid phase, with the gametic (haploid) phase being a relative few cells. Most of the cells in your body are diploid, germ line diploid cells will undergo meiosis to produce gametes, with fertilization closely following meiosis.Plant life cycles have two sequential phases that are termed alternation of generations. The sporophyte phase is "diploid", and is that part of the life cycle in which meiosis occurs. However, many plant species are thought to arise by polyploidy, and the use of "diploid" in the last sentence was meant to indicate that the greater number of chromosome sets occur in this phase. The gametophyte phase is "haploid", and is the part of the life cycle in which gametes are produced (by mitosis of haploid cells). In flowering plants (angiosperms) the multicelled visible plant (leaf, stem, etc.) is sporophyte, while pollen and ovaries contain the male and female gametophytes, respectively. Plant life cycles differ from animal ones by adding a phase (the haploid gametophyte) after meiosis and before the production of gametes.

Many protists and fungi have a haploid dominated life cycle. The dominant phase is haploid, while the diploid phase is only a few cells (often only the single celled zygote, as in Chlamydomonas ). Many protists reproduce by mitosis until their environment deteriorates, then they undergo sexual reproduction to produce a resting zygotic cyst.

Phases of Meiosis | Two successive nuclear divisions occur, Meiosis I (Reduction) and Meiosis II (Division). Meiosis produces 4 haploid cells. Mitosis produces 2 diploid cells. The old name for meiosis was reduction/ division. Meiosis I reduces the ploidy level from 2n to n (reduction) while Meiosis II divides the remaining set of chromosomes in a mitosis-like process (division). Most of the

differences between the processes occur during Meiosis I.

Comparison of Mitosis and Meiosis

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Mitosis maintains ploidy level, while meiosis reduces it. Meiosis may be considered a reduction phase followed by a slightly altered mitosis. Meiosis occurs in a relative few cells of a multicellular organism, while mitosis is more common.

Mitosis

This process of cellular division is unique to eukaryotic cells. The following illustrations depict only the replication of chromosomes and their division. Keep in mind that the process of mitosis involves the entire cell which includes a multitude of organelles.

Interphase Time between divisionsProtein synthesis carried outChromatin presentNucleolus presentDNA replicated towards division time

Prophase Chromatin thickens into chromosomesNuclear membrane disintegratesCentriole pairs move to opposite ends of the cellSpindle fibers begin to form

Metaphase Guided by the spindle fibers, the chromosome pairsline up along the center of the spindle structure

Anaphase The chromosome pairs (sisters) begin to pull apartOnce separated, they are called daughter chromosomesDue to pull, many chromosomes bendGroove in plasma membrane present

Telophase Chromosomes return to chromatinSpindle disintegratesNuclear membrane takes shape againCentrioles replicateMembrane continues to pinch inward(in plant cells a new cell wall is laid)

When the process is complete, each cell will have the same genetic material that the original cell had before replication. Each of the daughter cells is also identical to each other. Note that once telophase is complete, the cell returns to interphase.

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Meiosis

All gametes have half the number of chromosomes that regular cells have. Gametes are created through the process of meiosis. Meiosis involves two divisions which create four haploid cells.

Phase I

INTERPHASE I PROPHASE I METAPHASE I ANAPHASE I TELOPHASE I

In Phase I, all chromatin material makes a copy of itself. Spindle fibers begin to develop as the nuclear membrane disintegrates. Some crossing over may occur as the chromosomes thicken and group into tetrads. The tetrads line up and pull apart. Once the chromosomes pairs are at opposite poles the cytoplasm material divides. Note how the two cells formed do not have the same genetic material, yet they have the normal number of chromosomes.

Phase II

INTERPHASE II

PROPHASE II

METAPHASE II

ANAPHASE II

TELOPHASE II

In Phase II, no duplication of genetic material occurs. As the chromatin thickens to form chromosomes and group into pairs, the spindle forms and the

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nuclear membrane disintegrates. As in mitosis, the pairs of chromosomes line up at the center and pull apart to opposite poles. The cytoplams then divides. In male organisms the four new cells are all the same size. In females one of the four cells receives the bulk of the cytoplasm material. This becomes the functioning egg while the other three smaller cells disintegrate.

Cell theory:

Throughout time, thoughts and ideas of life have been formed, stretching from abiogenesis and spontaneous generation to the modern cell theory. Here is an overview of the progression of thought that has contributed to today's cell theory. You'll relive key events in scientific history and explore profiles of influential scientists and philosophers.

The Cell Theory

ANAXIMANDERA member of the Greeks in the sixth century B.C. who resided on the Ionian Islands. He is credited with coming up with the primary thoughts of evolution. His perspective was that creatures from the sea were forced to come ashore, thereby evolving into land creatures.

PLATOPlato did not directly aid in the progress of biological thinking. His view was not experimental, but more philosophical. Many of his students went on to influence the progression of biological studies in the field of classification.

THE ATOMISTSThe most noted of this group of Greek philosophers was Democritus (460 - 370 B.C.). He followed Anaximander's view of evolution. Democritus is credited as being the father of atomic theory which connects directly to biology. One important theory of his was simply that if you have nothing, nothing may be created out of it.

ARISTOTLEAristotle (384 - 322 B.C.) was known for his experimental approach and numerous dissections. He was drawn to animal classification in order to discover aspects of connection between the soul and the human body. Some of his animal classifications still stand today. One of his famous thoughts is a foreshadowing of Mendelian genetic concepts:

"It is evident that there must be something or other really existing, corresponding to what we call by the name of Nature. For a given germ does not give rise to any random living being, nor spring from any chance one, but each germ springs from a definite parent and gives rise to a predictable progeny. And thus it is the germ that is the ruling influence and fabricator of the offspring." THE DARK AGESFollowing the Greeks, there was a downfall in scientific thought. This decline is usually attributed to the Christian Church, and the power shift to the "barbaric" tribes.

LEONARDO DA VINCI, RENE DESCARTES, AND WILLIAM HARVEYThese three scientific figures, thought not all living during the same time period, can be accredited with

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much of the advancement of anatomical thought following the Dark Ages, such as discovering the circulation of blood.

THE MICROSCOPEThis instrument opened up new doors in the field of biology, by allowing scientists to gaze into a new world: the cellular world. Galileo is credited with the invention of the microscope. Two of the main pioneers in microscope usage were Athanasius Kircher and Antonie von Leeuwenhoek.

ROBERT HOOKEThis English naturalist (1635 - 1703) coined the term "cell" after viewing slices of cork through a microscope. The term came from the Latin word cella which means "storeroom" or "small container". He documented his work in the Micrographia, written in 1665.

JEAN-BAPTISTE DE LAMARCKThe majority of this Frenchman's work (1744 - 1829) dealt with animal classification and evolution. He is credited with taking steps towards the creation of the cell theory with this saying:

"Every step which Nature takes when making her direct creations consists in organizing into cellular tissue the minute masses of viscous or mucous substances that she finds at her disposal under favorable circumstances."

THE CELL: AN INDIVIDUAL UNIT OF LIFEIn 1824, Rene Dutrochet discovered that "the cell is the fundamental element in the structure of living bodies, forming both animals and plants through juxtaposition." However, the first sightings of the internal action of the cell were made by Robert Brown. In Berlin, Johannes Muller created connections between biology and medicine, prompting the connective thinking of his students, such as those of Theodore Schwann. Schwann created the term "cell theory" and declared that plants consisted of cells. This declaration was made after that of Matthias Schlieden's (1804 - 1881) that animals are composed of cells.

BIOGENESISGerman pathologist Rudolf Virchow (1821 - 1902) altered the thought of cellular biology with his statement that "every cell comes from a cell". Not even twenty years after this statement, processes of cell reproduction were being described--Virchow had completed the thought behind the basic cell theory

Useful Terms of Cellular Biology

Anaphase - phase of mitosis in which the chromosomes begin to pull to opposite poles of the cell Abiogenesis - spontaneous generation, nonliving matter yielding life Animal - kingdom composed of multicellular organisms divided into two divisions: vertebrates

and invertebrates, who obtain their food from external sources and reproduce sexually or asexually Asexual - a type of reproduction that does not require the union of female and male gametes Biogenesis - the concept that all life arises from living matter Cell - the smallest unit of life that carries out its own processes Cellulose - a carbohydrate that is found in cell walls Cell wall - multi-layered, sturdy structure composed of cellulose that provides plants and other

organisms with their rigidity Centrioles - essential tubular organelles found near the nucleus in pairs that aid in cellular

division Chlorophyll - the green material found in chloroplasts that is active in photosynthesis

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Chloroplasts - membrane-bound organelles containing chlorophyll that is found in photosynthetic organisms

Chromosomes - condensed form of chromatin visible during cellular division Cytoplasm - collective term for cytosol and all the organelles contained in it (outside the nucleus

and within the plasma membrane) Cytoskeleton - network of microtubules that support and give structure to cell while aiding in

intracellular transport Cytosol - jelly-like material that contains the organelles between the nucleus and the plasma

membrane DNA - deoxyribonucleic acid is the double-helix molecule holding the genetic information of

organisms that, along with protein, composes the chromatin Endoplasmic reticulum - the cell's highway transport system composed of tubes and membranes

connected to the nuclear membrane and extending through the cytoplasm that is also utilized for storing and separating

Enzyme - a protein utilized in chemical reactions Eukaryotes - advanced cell type with a nuclear membrane surrounding genetic material and

numerous membrane-bound organelles dispersed in a complex cellular structure Flagellum - an extension on many unicellular organisms that enables movement through whip-

like motions Fungi - kingdom in which organisms may be unicellular or multicellular, obtain their food from

external sources, and reproduce sexual or asexually Golgi apparatus - multi-layered organelle near the nucleus used for packaging of materials to be

transported out of the cell Interphase - time period between cellular divisions in which cellular processes such as protein

synthesis are carried out Lysosomes - the digestive plants of food for the cell, changes shape from task to task Meiosis - cellular division that yields four gametes through two cellular divisions Metaphase - phase of mitosis in which the chromosome pairs line up at the equator of the cell Mitochondria - genetically independent organelles that produce energy for the cells along their

many internal folds, called cristae Mitosis - cellular division that yields two identical cells from one cell through a five-step process Moneran - simplest kingdom containing prokaryotic cells (mostly bacteria), some of which can

create their own food Nuclear membrane - membrane surrounding the nucleus that is covered with pores and controls

nuclear traffic Nucleolus - spherical structure within the nucleus that contains RNA Nucleus - spherical organelle that is the cell's control center Phagocyte - collective term for cells that engulf other cells or microorganisms Plant - kingdom containing a variety of multicellular organelles that reproduce sexually or

asexually, and also carry out photosynthesis Plasma membrane - outer membrane of cells composed of proteins and a phospholipid bi-layer

that controls cellular traffic Plastid - vital organelle that aids in the metabolism of unicellular organisms and plant cells

(chloroplasts, chromoplasts, leucoplasts are examples) Prokaryotes - primitive cell type that lacks a nuclear membrane and membrane-bound organelles Protein - a complex molecule found in numerous cellular structures that is composed of amino

acids Protist cell - kingdom containing organisms with eukaryotic cells that are unicellular and

multicellular and reproduce sexually and asexually Prophase - phase of mitosis in which the chromatin duplicates itself and thickens into

chromosomes, the spindle fibers form, and the nuclear membrane disintegrates Ribosomes - extremely small grain-like organelle that provides the sites for protein synthesis

(they may be free in the cytoplasm or attached to the endoplasmic reticulum) RNA - ribonucleic acid, a molecule that is a necessary component of the protein synthesis process Sexual - reproduction involving the union of female and male gametes to form a zygote Spontaneous generation - abiogenesis, the creation of life from nonliving material

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Telophase - phase of mitosis in which the chromosome pairs have separated and reached opposite poles of the cell as the spindle begins to disintegrate, the nuclear membrane reappears, and the cytoplasm begins to divide

Vacuoles - membrane-bound organelles in the cytoplasm that are used for storage and digestion

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