Week 6

Agenda 9/26• Review Metabolism & Free Energy Worksheet

and practice quiz – 10 min

• Enzymes (slides 35-62) with virtual demos and guided notes

• Homework – – 6.1 and 6.2 Guided Notes Worksheet due tomorrow– Cornell Notes Ch. 6 and self-quiz due tomorrow

Enzyme Animation

• http://www.lpscience.fatcow.com/jwanamaker/animations/Enzyme%20activity.html

• http://www.kscience.co.uk/animations/model.swf• Setup each and then record trend:

– 1) control E(5), S(20), T(40), pH(7) vol(300)– 2) increase enzyme (15)– 3) increase substrate (40)– 4) non-optimal temp (0 and 60)– 5) non-optimal pH (10) Note: this particular model uses an

enzyme that favors acid pH– Then try E(10), S(60) and rest as for control – notice how

rate of reaction slows

In general…

• More enzyme = faster reaction rate

• More substrate up to a certain point = faster reaction rate

• Too high/too low temp = slower reaction rate

• Too high/too low pH = slower reaction rate

• With inhibitors= slower reaction rate

Must Know/Beautiful things about enzymes

• They can be used over and over- 1000’s of substrate molecules can be changed per second…

• They are very specific meaning they are an expert at only one reaction. Won’t do anything else.

• They do not perform magic – they don’t change the rules of the reaction (equilibrium or delta G) – we just get there faster.

Agenda 9/27• Finish/Review Enzymes• Toothpickase Lab – I’ll walk you through first 2

counts, then you do on your own while I check notes during this– Run for a total of 4 minutes (After step 13, count for

120 more seconds)– Share data and go over discussion questions

Homework-– Print out and read Lab 2 Enzyme Catalysis from

website (different lab - not the one in your lab manual!) - Prelab – Identify control, independent variable etc. for part A and B and know procedure!!! Then answer questions 1-6 at end. Use this link to become familiar with setup http://www.explorebiology.com/apbiology/labs/lab08.html

– Toothpickase worksheet with graph due tomorrow

Agenda 9/28

• Run part A and B – do 8 minute runs instead of 5 minutes (for B – some groups start with 0.25 and some with 0.75)

• Assign groups parts C,D,E or F for tomorrow• Homework –

– Prelab – Identify control, independent variable etc. for part you are assigned for tomorrow and know procedure!!! Look over what other groups doing

– Graph A and B on one graph together – label different lines, then answer question 7a-c

Agenda 9/29

• Run parts C, D, E or F • Graph data and answer questions for your

section

• Homework –– Finish graphing as needed for your part– Try to answer as many of questions as you

can – especially for your part and #12

Agenda 9/30

• Go through Analysis Questions• Share out group data – discuss errors, etc.

• Homework-• Finish worksheet for Lab 2 and Free Response

questions due Monday• AP Lab 2 Report due next Wed. 10/5• Enzyme Quiz on Monday