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Welcome Address

We are honoured and pleased to welcome over 200 participants and guests from eighteen different

Countries, at the International Symposium on the European outbreak of Xylella fastidiosa in olive, held in

Apulia, Italy.

The Scientific Committee with the collaboration on the invited speakers have built a scientific program which

will provide an in depth and updated overview on this harmful pathogen, and illustrate the current situation

in the outbreak area of Apulia. The researchers directly involved in the ongoing research activities on this

new emerging threat will share, the knowledge and the data so far gathered, with the international scientific

community and critically discuss the future steps and efforts to boost the ongoing research programs.

As a complement to the core scientific program, the National and the Regional Plant Health Services have

organized a specific session for the presentation and discussion of the phytosanitary and legislative

measures to be adopted and implemented.

The Symposium offers, with the field trip, a unique chance to visit the diseased area and have a look of the

incidence and severity of the symptoms.

We are delighted to invite a broad scientific community to this symposium. We very much hope that you will

share our enthusiasm for this Symposium and we thank everybody who have decided to join us in this

beautiful area.

The Organizing Committee

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Congress Board SCIENTIFIC COMMITTEE

Giovanni Paolo MARTELLI University of Bari Aldo Moro, Bari, Italy

Rodrigo ALMEIDA University of California, Berkeley, USA

Domenico BOSCO University of Turin, Italy

Jacob Dirk JANSE Dutch Inspection Service, Emmeloord, The Netherlands

Alexander H. PURCELL University of California, Berkeley, USA

ORGANIZING COMMITTEE

Donato BOSCIA CNR Institute for Sustainable Plant Protection, Bari, Italy

Michele DIGIARO CIHEAM Mediterranean Agronomic Institute, Bari, Italy

Bruno Caio FARAGLIA Ministry of Agricultural, Food and Forestry Policies, Italy

Antonio GUARIO Regional Phytosanitary Service, Apulia, Italy

Franco NIGRO University of Bari Aldo Moro, Bari, Italy

Francesco PORCELLI University of Bari Aldo Moro, Bari, Italy

Vito Nicola SAVINO University of Bari Aldo Moro, Bari, Italy

CONGRESS SECRETARIAT

Giuliana LOCONSOLE CNR Institute for Sustainable Plant Protection, Bari, Italy

Francesco PALMISANO CRSFA “Basile Caramia”, Locorotondo (Bari), Italy

Maria SAPONARI CNR Institute for Sustainable Plant Protection, Bari, Italy

ADMINISTRATIVE SECRETARIAT

Grazia Valentina TRITTO CNR Institute for Sustainable Plant Protection, Bari, Italy

Maria Grazia PIEPOLI CRSFA “Basile Caramia”, Locorotondo (Bari), Italy

EDITORIAL GRAPHIC DESIGNER

Massimiliano MORELLI CNR Institute for Sustainable Plant Protection, Bari, Italy

WEBMASTERS

Simona ABBÀ CNR Institute for Sustainable Plant Protection, Torino, Italy

Pasquale SALDARELLI CNR Institute for Sustainable Plant Protection, Bari, Italy

COLLABORATORS

Oriana POTERE

Giovanna BOTTALICO

Daniele CORNARA

Leonardo SUSCA

Antonio CEGLIE University of Bari Aldo Moro, Bari, Italy

Michela CHIUMENTI

Angelo DE STRADIS CNR Institute for Sustainable Plant Protection, Bari, Italy

Toufic ELBEAINO

Franco VALENTINI

Peter MOUBARAK CIHEAM Mediterranean Agronomic Institute, Bari, Italy

Antonella SAPONARI

Leonardo CALAPRICO

Leonardo CATUCCI

Maria FASANO

Milena GUIDA

Vito MONTILON

Donato PALMISANO

Milena PETRUZZI

Maria PINTO

Paola POLLASTRO

Maria Rosaria SILLETTI

Nicola TRISCIUZZI

Giuseppe ALTAMURA CRSFA “Basile Caramia”, Locorotondo (Bari), Italy

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Acknowledgements

The INTERNATIONAL SYMPOSIUM ON THE EUROPEAN OUTBREAK OF XYLELLA FASTIDIOSA IN OLIVE has been

organized by:

CNR INSTITUTE FOR SUSTAINABLE PLANT PROTECTION, BARI, ITALY

DEPARTMENT OF SOIL, PLANT AND FOOD SCIENCE, UNIVERSITY OF BARI ALDO MORO, BARI, ITALY

CRSFA RESEARCH CENTER “BASILE CARAMIA”, LOCOROTONDO (BARI), ITALY

CIHEAM MEDITERRANEAN AGRONOMIC INSTITUTE, BARI, ITALY

The Organizing Committee would like to thank the following Italian institutions which have provided funds to

develop this event:

MINISTERO PER LE POLITICHE AGRICOLE ALIMENTARI E FORESTALI

REGIONE PUGLIA–AREA POLITICHE PER LO SVILUPPO RURALE-SERVIZIO AGRICOLTURA-OSSERVATORIO

FITOSANITARIO REGIONALE

The Organizing Committee wishes to acknowledge the invited speaker for their invaluable scientific

contributions and Prof. G. P. Martelli for precious help with abstract editing.

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The Organizing Committee wishes to thank the following organizations for their generous ongoing support:

GOLD SPONSORS

The Organizing Committee is also grateful to the following organization for their kind participation:

SILVER SPONSORS

BRONZE SPONSORS

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Key Information

CONGRESS SECRETARIAT

Dr. Maria Saponari, Dr. Giuliana Loconsole, Dr. Francesco Palmisano

Mail to: xylella.symposium@ba.ivv.cnr.it.

During the congress, the Secretariat can be reached at +39.3403424074

CONGRESS VENUES

Symposium sessions

Costa Brada Luxury Resort, Litoranea Santa Maria di Leuca, Gallipoli (Lecce)

Web: www.costabradaresort.it | Tel: +39.0833.202551 | Mail to: info@costabradaresort.it

Workshop sessions

CRSFA Centro di Ricerca, Sperimentazione e Formazione in Agricoltura “Basile Caramia”

Via Cisternino, 281, Locorotondo (Bari)

Web: www.crsfa.it | Tel. +39.080.4313071 | Mail to: info@crsfa.it

WEB SITE

The Congress official website can be reached at: www.ivv.cnr.it/symposium-xylella-fastidiosa

ACCREDITATION PROCEDURES

Symposium sessions are open to all registered participants. Your ID badge included in delegate‟s bags is

required for admission to the Congress Centre and all Congress events. All enquiries should be directed to

the registration helpdesk in the entrance hall.

Registration Opening Hours:

Monday 20th October 17:00−19:00 | Tuesday 21st October 8:30−18:30 | Wednsday 22nd October 8:30−12:30.

CERTIFICATE OF ATTENDANCE

Certificate of Attendance will be included in the delegate‟s bag.

LANGUAGE

English is the Congress official language. Simultaneous tanslation will be available.

INTERNET ACCESS

General Wi-Fi access is available throughout the Congress Venue. Access password will be provided upon

your request.

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POSTERS AND ORAL PRESENTATIONS

Posters will be on display during symposium sessions in the dedicated area. Poster presenters are asked

to mount their poster before the opening ceremony. Posters must be removed at the end of symposium

sessions. Any posters remaining after this time will be removed by the organisers and cannot be

reclaimed.

Power Point Presentations, on USB flash drives, should be handed to the representative of the Congress

Secretariat in advance but not later than 15 min prior to the start of the session.

CONGRESS SOCIAL DINNER

Congress Dinner will take place on Tuesday 21st October at the Masseria Li Sauli, Gallipoli (www.lisauli.it).

Participants will be asked to present tickets found in delegate‟s bag upon arrival at the event venue.

FIELD TRIP

During field trip, scheduled on Wednesday 22nd October (14:00-17:00) participants will have opportunity to

visit olive groves in the area were the main Xylella fastidiosa outbreak was first reported in October 2013.

Please inform the Congress Secretariat if you are not willing to attend the field trip.

SHUTTLE BUS SERVICE

Complimentary shuttle bus service from Brindisi/Bari airports to Gallipoli and viceversa will be available

for participants attending the Symposium during its entire duration. Please at the airport look for the sign

“Xylella”. On Thursday 23 October a shuttles bus will be available from Gallipoli to CRSFA Research Center

(Locorotondo) for participants attending workshop training sessions.

On Friday 24th October, and upon kind request on Saturday 25th October, shuttle bus service will

accompany participants leaving workshop sessions to Brindisi/Bari airports.

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Touristic Tips

CONGRESS LOCATIONS

SYMPOSIUM LOCATION:GALLIPOLI (LECCE)

Located on the southeastern coast of the „‟Italian heel‟‟, Gallipoli is celebrated for its crystal clear blue sea

and the sandy beaches that make its territory one of the most cherished natural attractions in Salento.

When viewed from the sea, Gallipoli‟s white skyline reveals itself in all of its oriental beauty, resembling a

village of the Cyclades islands. The old town, perched on a limestone islet, is connected to the mainland via

an arched bridge. The walls, ramparts and towers that once defended the city from foreign raiders, now

provide shelter from the rough sea and confer the city a special charm that takes visitors back in time.

Don‟t miss to visit the baroque cathedral dedicated to Saint Agatha and the Hellenistic fountain close to the

bridge, rich in bas-reliefs depicting scenes from Greek mythology, and the Sanctuary of Our Lady of

Canneto. The city‟s fascination adds to its beautiful nature, offering long beaches of very fine white sand

that are mostly cherished by family vacationers and water sports fans.

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WORKSHOP LOCATION: LOCOROTONDO (BARI)

The village is located in Valle d‟Itria and you can enjoy the typical rural landscape from various sight of the

historic centre with his small “trulli” locally called “casedde” and farms. A center of concentric streets,

low houses with the typical pointed roof and whitewashed, originally built to fight against the sun and the

pestilence, and then become the symbol of the country, the “cummerse“.

Entering at the city centre by Napoli‟s door, you are immediately greeted by an efficient tourist

information service, where you can find all the information materials, in various languages, to better

organize your visit. Then you go in the nice built-up area made pleasant by the open spaces and by the

white houses with main entrance and railings profiles stone. The food and wine is strongly linked to the

local tradition: the white wine Locorotondo DOC and oil, and dishes such as orecchiette with broccoli and

the mashed fava beans with wild chicory.

In the midway between Cisterino and Alberobello, three are the stage unmissable (all certified with the

Orange flag by Touring Club Italiano) a cultural and culinary excellence itinerary through the Itria valley.

PROGRAMME SCHEDULE SYMPOSIUM SESSIONS

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Monday, 20th October

Tuesday, 21st October

17:00-19:00 REGISTRATION AND POSTER HANGING PROCEDURES

19:00-21:00 WELCOME RECEPTION AT COSTA BRADA RESORT, GALLIPOLI (LECCE)

08:30-09:00 REGISTRATION AND POSTER HANGING PROCEDURES

09:00-09:30 WELCOME

G. P. Martelli, Professor Emeritus, Department of Soil, Plant and Food Science, University of Bari Aldo Moro, Italy

E. Lavarra, Representative of the Italian Minister of Agricultural, Food and Forestry

Policies

N. Vendola, President of the Apulia Regional Government

F. Nardoni, Regional Assessor for Agriculture

G. P. Accotto, Director of the CNR Institute for Sustainable Plant Protection

A. Uricchio, Rector of the Aldo Moro University of Bari

C. Lacirignola, General Secretary of the CIHEAM Mediterranean Agronomic Institute , Bari

A.Palmisano, President of the CRSFA Research Center “B. Caramia”, Locorotondo (Bari)

SESSION I XYELLA FASTIDIOSA: THE PATHOGEN, ITS EPIDEMIOLOGY AND MANAGEMENT Chairman: Prof. G. P. Martelli

09:30-10:00 HISTORICAL PERSPECTIVES ON XYLELLA FASTIDIOSA AND THEIR RELEVANCE FOR THE

FUTURE

A. H. Purcell, Professor Emeritus, University of California, Berkeley, USA

10:00-10:30 DISEASES INDUCED BY XYLELLA FASTIDIOSA SUBSP. PAUCA: ECOLOGY, EPIDEMIOLOGY AND

MANAGEMENT

H. Della Coletta Filho, Centro de Citricultura Sylvio Moreira, IAC Cordeirópolis, São Paulo, Brazil

10:30-10:50 POTENTIAL VECTORS OF XYLELLA FASTIDIOSA IN EUROPE

D. Bosco, R. Almeida, E. Czwienczek, G. Stancanelli, J. C. Gregoire, D. Caffier, G. Hollo, C. Bragard

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10:50-11:10 COFFEE BREAK

11:10-11:40 CONTROL STRATEGIES FOR XYLELLA FASTIDIOSA

D. L. Hopkins, University of Florida, Central Florida Research and Education Center, USA 11:40-12:10 CELL DENSITY-DEPENDENT BEHAVIORS OF XYLELLA FASTIDIOSA: ACHIEVING DISEASE

CONTROL VIA PATHOGEN CONFUSION

S. E. Lindow, University of California, Berkeley, USA 12:10-12:30 SOME APPROACHES AIMING CITRUS VARIEGATED CHLOROSIS CONTROL IN BRAZIL

A. A. de Souza, Centro de Citricultura Sylvio Moreira, IAC Cordeirópolis, São Paulo, Brazil

SESSION II OLIVE QUICK DECLINE SYNDROME AND XYLELLA FASTIDIOSA EPIDEMICS IN

APULIA Chairman: Prof. A. H. Purcell

12:30-13:00 THE OLIVE QUICK DECLINE SYNDROME

G. P. Martelli, Professor Emeritus, Dept. of Soil, Plant and Food Science, University of Bari Aldo Moro, Italy

13:00-13:20 IDENTIFICATION AND CHARACTERIZATION OF FUNGAL SPECIES ASSOCIATED WITH THE QUICK

DECLINE SYNDROME OF OLIVE

F. Nigro, I. Antelmi, A. Ippolito, Dept. of Soil, Plant and Food Science, University of Bari Aldo Moro, Italy

13:20-13:40 OCCURENCE OF XYLELLA FASTIDIOSA IN APULIA

D. Boscia, CNR Institute for Sustainable Plant Protection, UOS Bari, Italy

13:40-15:00 LUNCH

15:00-15:15 SURVEY OF THE AUCHENORRHYNCHA IN THE SALENTO PENINSULA IN SEARCH OF PUTATIVE

VECTORS OF XYLELLA FASTIDIOSA STRAIN CODIRO

D. Cornara, G. Loconsole, D. Boscia, A. De Stradis, R. Y. Yokomi, D. Bosco, F. Porcelli, M. Saponari

15:15-15:30 OBSERVATIONS ON THE BIOLOGY AND ETHOLOGY OF APHROPHORIDAE: PHILAENUS SPUMARIUS (L.) IN THE SALENTO PENINSULA

D. Cornara, F. Porcelli, Dept. of Soil, Plant and Food Science, University of Bari, Italy

SESSION III DETECTION AND CHARACTERIZATION OF XYELLA FASTIDIOSA Chairman: Prof. J. D. Janse

15:30-15:50 AN INNOVATIVE MONITORING MODEL FOR XYLELLA FASTIDIOSA IN APULIA REGION, ITALY

A. M. D‟ Onghia, F. Santoro, T. Yaseen, K. Djelouah, A. Guario, A. Percoco, T. Caroppo, F.

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Valentini

15:50-16:10 MODELLING THE SPREAD OF XYLELLA FASTIDIOSA IN APULIA (SOUTHERN ITALY)

S. M. White, J. M. Bullock, D. A. P. Hooftman, D. S. Chapman 16:10-16:30 INTERLABORATORY VALIDATION OF MOLECULAR AND SEROLOGICAL DIAGNOSIS OF XYLELLA

FASTIDIOSA STRAIN CODIRO IN SUSCEPTIBLE HOSTS

G. Loconsole, O. Potere, T. Elbeaino, D. Frasheri, S. Frisullo, F. Palmisano, D. Boscia, M. Saponari

16:30-16:50 COFFEE BREAK

16:50-17:10 ISOLATION, GENETICS AND PRELIMINARY DATA ON THE PATHOGENICITY OF THE XYLELLA FASTIDIOSA CODIRO STRAIN

M. Saponari, G. Loconsole, R. Almeida, H. Della Coletta Filho, G. P. Martelli, D. Boscia 17:10-17:30 DRAFT GENOME SEQUENCE OF XYLELLA FASTIDIOSA STRAIN CODIRO

A. Giampetruzzi, M. Chiumenti, M. Saponari, G. Donvito, A. Italiano, G. Loconsole, C. Cariddi, G. P. Martelli, P. Saldarelli

17:30-17:50 IDENTIFICATION AND CHARACTERIZATION OF XYLELLA FASTIDIOSA ISOLATED FROM COFFEE

PLANTS IN FRANCE B. Legendre, S. Mississipi, V. Olivier, E. Morel, D. Crouzillat, K. Durand, P. Portier, F.

Poliakoff, M. A. Jacques

17:50-18:30 POSTER VIEWING SESSION

20:00 SOCIAL DINNER AT MASSERIA LI SAULI, GALLIPOLI (LECCE)

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Wednesday, 22nd October

SESSION IV CURRENT ACTIONS FOR XYELLA FASTIDIOSA CONTROL Chairmen: B. C. Faraglia; G. Papa Pagliardini

08:30-08:45 OPENING REMARKS

V. N. Savino, Dept. of Soil, Plant and Food Science, University of Bari Aldo Moro, Italy 08:45-09:15 PHYTOSANITARY REGULATION AGAINST XYLELLA FASTIDIOSA FROM A EUROPEAN UNION

PERSPECTIVE

G. Cardon, Directorate General for Health and Consumers, European Commission 09:15-09:45 MEASURES TAKEN BY THE ITALIAN PLANT PROTECTION SERVICE

B. C. Faraglia, Ministry of Agricultural, Food and Forestry Policies, Italy A. Guario, Rural Development Policy Area, Regional Phytosanitary Service, Apulia, Italy

09:45-10:15 Risk assessment of Xylella fastidiosa for the EU

G. Stancanelli, R. Almeida, D. Bosco, C. Bragard, D. Caffier, J. C. Gregoire, S. Parnell, G. Strona, O. Mosbach-Schulz, E. Czwienczek, G. Hollo

10:15-10:45 COFFEE BREAK

10:45-11:15 EPPO SUPPORTING THE EVOLUTION OF PHYTOSANITARY SYSTEMS IN EPPO MEMBER

COUNTRIES

F. Petter, F. Grousset & M. Suffert, EPPO European and Mediterranean Plant Protection Organization

11:15-12:15 OPEN DISCUSSION

12:15-12:30 CONCLUDING REMARKS

M. Barba, Coordinator of the Advisory Scientific Committee for Xylella fastidiosa

12:30-13:30 LUNCH

14:00-17:30 FIELD TRIP

19:00-21.00 OLIVE PICKING TRIP: NIGHT HARVESTING OF OLIVES

21.00 BUFFET AT ANTICA MASSERIA DEL FANO, SALVE (LECCE)

PROGRAMME SCHEDULE WORKSHOP SESSIONS

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Thursday, 23th October

07:30- DEPARTURE FOR LOCOROTONDO (BARI) FOR THE TRAINING WORKSHOPS

09:30-10:00 COFFEE BREAK TRAINING WORKSHOP A

CURRENT TOOLS FOR THE DETECTION OF XYLELLA FASTIDIOSA IN HOST PLANTS

AND VECTORS

10:00-11:00 PRESENTATION OF THE COURSE AND OVERVIEW ON THE DIAGNOSTIC TOOLS

M. Saponari, G. Loconsole, O. Potere, F. Palmisano, F. Valentini, H. D. Coletta Filho, D. Boscia

11:00-13:00 LABORATORY WORK: ELISA, DNA ISOLATION, PCR AND QPCR

13:00-14:30 LUNCH

14:30-18:00 LABORATORY WORK: ELISA, DNA ISOLATION, PCR AND QPCR (CONTINUATION)

TRAINING WORKSHOP B CHARACTERISTICS AND IDENTIFICATION OF XYLEM-SAP FEEDERS

10:00-11:00 PRESENTATION OF THE COURSE AND OVERVIEW ON TAXONOMY AND BIOLOGY OF INSECT

VECTORS OF XILELLA FASTIDIOSA

A. H. Purcell, D. Bosco, F. Porcelli, D. Cornara, L. Picciau 11:00-13:00 LABORATORY WORK

13:00-14:30 LUNCH

14:30-18:00 LABORATORY WORK AND FIELD SESSION

19:30-20:30 VISIT TO A LOCAL WINERY

20:30 DINNER AT A LOCAL RESTAURANT

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Friday, 24th October

TRAINING WORKSHOPS A-B

08:30-16:30 LABORATORY WORK (CONTINUATION)

12:45-14:00 LUNCH

16:00-16.30 DISCUSSION AND CONCLUDING REMARKS