What is the difference between a Screening and a Confirmation Test?

SCREENING TEST In a screening test, the sample is tested only once per plate. Advantage: This format is easy-to-use and cost-effective. In the IDvet range, the screening tests have product codes which end in “S” = PARAS, SBVS, VISNAS.

CONFIRMATION TEST (also known as verification test)

If scientists wish to confirm positive results obtained with an indirect screening ELISA, they should use a different technique to do so, such as IFAT, VNT, HAI or another ELISA method (competitive ELISA instead of indirect ELISA, for example). Strictly speaking, a confirmation ELISA does not confirm a positive result; because it is based on the same indirect ELISA as the screening ELISA. Instead, it demonstrates that the positive result is due to specific binding of the sample antibody to the antigen, and not due to other factors (non-specific binding of the antibodies to the plastic or saturation buffer, for example). Non-specific binding may occur in rare cases, such as when testing contaminated or strongly haemolysed sera. In most cases in the IDvet range, the coated well in the confirmation test is the same as that of the screening test In the IDvet range, the confirmation tests have product codes which end in “B” = PARAB, SBVB, VISNAB

96 reactions per plate

48 reactions per plate