what lives in your water? new mdbiolab activity
DESCRIPTION
What Lives In Your Water? New MdBioLab Activity. G. Houston-Ludlam Graduate Student CBMG, UMCP. Our Activity. Step 1- Collect water samples Field trip or Homework Students should work in pairs Will require a “collection kit” Clean plastic bottles Gloves - PowerPoint PPT PresentationTRANSCRIPT
What Lives In Your Water?New MdBioLab Activity
G. Houston-LudlamGraduate Student
CBMG, UMCP
Our Activity• Step 1- Collect water
samples– Field trip or Homework
• Students should work in pairs
• Will require a “collection kit”– Clean plastic bottles– Gloves– Ziplocs for ice and
containment of sample
http://ian.umces.edu/imagelibrary/albums/userpics/10025/normal_iil_ian_bf_395.JPG
Our Activity
• Step 2- Filter water samples and culture overnight– 2 different volumes
• 10 ml• 100 ml• Allows for best
opportunity to get a countable plate of 20-60 colonies
http://www.umesc.usgs.gov/aquatic/drug_research/capabilities.html
Our Activity
• Step 3- (Next Day) Count Colonies
Example of bacterial growth on selective media. Photo courtesy of Hornor Lab, Anne Arundel Community College, Arnold, MD.
Equipment Setup• Completely assembled
filtration apparatus• Water samples in ice
bucket• Field data sheet• Sterile 10 ml syringe or
pipet• Beaker with ethanol
holding forceps• Sterile paper filter
Place Filter 1
• Peel cover off filter (best done by instructor or partner)
• Grab edge with sterilized forceps
Place Filter 2• Place paper filter grid
side up on top of metal screen
• Paper must completely cover screen to get proper filtration
Reassemble Filtration Apparatus
• Place filter funnel on top of paper filter
• Clamp glassware in place
10 ml Sample
• Wet filter with 10 ml sterile, distilled water– Water removes static
from syringe• When the water has
suctioned through filter, apply 10 ml of water sample to filter
Wash Filter Funnel
• With clean syringe, wash the sides of the funnel to get any splashes
Remove Filter
• Unclamp filter funnel• Grab edge of filter with
sterile forceps and break vacuum seal
Place on Plate
• Hold plate tilted downward and away
• Place filter at bottom edge of plate
• Roll onto media to minimize bubbles
• Cover and incubate 24 hrs
Repeat for 100 ml
• Place new filter on filtration apparatus
• Wet filter and suction through
• Pour 100 ml into funnel• Wash sides of funnel• Place filter on media
After Incubation• This is what the students will
see after a 24 hour incubation at 41˚C (chicken body temperature)
• Left-hand plates came from Patuxent River
• Right-hand plates came from Warehouse Creek off South River
• Top plates are 10 ml, bottom plates are 100ml samples