when labeling a petri plate you should write on the: 1.side 2.bottom 3.top 4.inside of the bottom
TRANSCRIPT
When labeling a petri plate you should write on the:
Side
Bottom Top
Insid
e of the ...
0% 0%
33%
67%1. Side2. Bottom3. Top4. Inside of the bottom
Plasmids are called ____ when they carry a desired gene into recipient cells.
Parasit
es
Vectors
Cultured
Bacte
ria
0%7%
14%
79%1. Parasites2. Vectors3. Cultured4. Bacteria
Bacteria are often classified according to cell shape—round, rod, and spiral. Which of the following is rod
shaped?
Diploco
ccus P
neumoniae
Staphylo
cocc
us Aure
us
Stre
ptoco
ccus H
emolyticus
Bacillu
s Subtilis
0%
100%
0%0%
1. Diplococcus Pneumoniae
2. Staphylococcus Aureus
3. Streptococcus Hemolyticus
4. Bacillus Subtilis
Each of the following is a direct positive outcome of the genetic modification of crop
plants EXCEPT:
Herb
icide Resi.
..
Pest Resis
tanc...
Enhan
ced ch
lor...
Vaccine En
rich...
29%
36%36%
0%
1. Herbicide Resistance
2. Pest Resistance3. Enhanced
chlorophyll production
4. Vaccine Enrichment
What component used in protein gel electrophoresis causes the proteins to run
toward the positive electrode?
Polyacry
lamide
The Tris in
the buffer
The glycin
e in th
e buffer
The SDS
29%
36%
7%
29%
1. Polyacrylamide2. The Tris in the
buffer3. The glycine in the
buffer4. The SDS
A particular processed mRNA molecule is 704 nucleotides long. How many amino acids are most likely to be in the protein
resulting from the translation of this mRNA molecule?
2112230
703704
7%0%
7%
86%1. 21122. 2303. 7034. 704
Eukaryotic cells and prokaryotic cells are different in that eukaryotic cells:
Do not h
ave or..
.
Are sm
aller t
h...
Have
a nuclear..
.
Do not h
ave D
N...
0% 0%
100%
0%
1. Do not have organelles
2. Are smaller than prokaryotic cells
3. Have a nuclear membrane
4. Do not have DNA
In DNA gel electrophoresis the ____ fragments migrate the ____ distances and the ____
fragments migrate the ____ distances.
Shorte
st/gre
at...
Longe
st/greate
...
Nonpolar
/great..
.
Polar/greate
st...
64%
7%7%
21%
1. Shortest/greatest/longest/least
2. Longest/greatest/shortest/least
3. Nonpolar/greatest/polar/least
4. Polar/greatest/Nonpolar/least
Which piece of equipment would be used to separate bacteria from the media they are in?
Autocla
ve
Thermal C
ycler
Centrifu
ge
Electr
ophoresi.
..
8%
23%23%
46%1. Autoclave2. Thermal Cycler3. Centrifuge4. Electrophoresis
The solution used to sterilize the bench top when you have completed your experiment is:
10% bleach so
l...
Hyd
roch
loric A
... So
ap
50% bleach
86%
14%
0%0%
1. 10% bleach solution2. Hydrochloric Acid3. Soap4. 50% bleach
The biotechnology product that came from the hot springs of Yellowstone National Park is:
DNA lig
ase
RNA polymera
se
Thermus A
quaticus D
NA...
Bacillu
s Sulfo
lobus kinase
7%
36%
50%
7%
1. DNA ligase2. RNA polymerase3. Thermus Aquaticus
DNA polymerase4. Bacillus Sulfolobus
kinase
The mole establishes a relationship between the:
Atomic
mass un...
The size
of an...
The molecu
lar ...
The charge
of ...
64%
0%0%
36%
1. Atomic mass unit and the gram
2. The size of an atom and its mass
3. The molecular mass of a compound and empirical formula
4. The charge of an atom and its ionization energy
The building blocks of proteins are:
Nucle
otides
Amino acids
Deoxyri
bose m
olecules
suga
rs
0% 0%7%
93%1. Nucleotides2. Amino acids3. Deoxyribose
molecules4. sugars
What type of bond is found between water molecules?
Ionic
Covalent
Hyd
rogen
Van der Waa
ls
21%
0%
57%
21%
1. Ionic2. Covalent3. Hydrogen4. Van der Waals
What enzyme is responsible for carrying DNA replication?
RNA polymera
se
DNA polym
erase
DNA lig
ase
RNA ligase
14%7%
0%
79%1. RNA polymerase2. DNA polymerase3. DNA ligase4. RNA ligase
In preparing agar plate growth media, the best time to add antibiotics is:
Before auto
cla...
Immediate
ly fo
...
Immediate
ly be...
After plates h
...
7% 7%
57%
29%
1. Before autoclaving so as to sterilize antibiotics
2. Immediately following autoclaving while medium is still hot
3. Immediately before pouring while media is cooled but still liquid
4. After plates have been poured and cooled
DNA travels from the ____ to the _____ terminal during gel electrophoresis.
Positive
/nega
t...
Neutra
l/negati...
Neutra
l/positi
...
Negative/p
osit...
21%
71%
7%0%
1. Positive/negative2. Neutral/negative3. Neutral/positive4. Negative/positive
The structure that acts as the cell’s security guard regulating what enters and leaves the cell
is known as the:
Nucle
us
Golgi b
odies
Cell membra
ne
Prote
in barrie...
7%0%
93%
0%
1. Nucleus2. Golgi bodies3. Cell membrane4. Protein barrier
PCR primers are short lengths of single-stranded DNA that are specifically designed to compliment, and anneal to, short base sequences that flank the PCR’s “target” DNA. Identify the
best primer to use in a PCR reaction that includes the following DNA sample: 3’ GAGTACCGATTAACACGGTAtargetregionCAGGATACGAGCTCTACCG 5’
5’ ATGGCTAATTG...
3’ ATGGCTAATTG...
3’ TATGCTCGAGA...
5’ TAAT 3’
79%
7%7%7%
1. 5’ ATGGCTAATTGTGC 3’2. 3’ ATGGCTAATTGTGC 5’3. 3’ TATGCTCGAGAT 5’4. 5’ TAAT 3’
There are 3 types of RNA and each type is involved in the synthesis of proteins. The first type is part of the ribosome, the second type is the template for translation, and
the third type brings amino acids to the ribosome. Which answer below lists these three types of RNA in the same order as described in the question?
mRNA, tR
NA, rRNA
rRNA, tR
NA, mRNA
tRNA, m
RNA, rRNA
rRNA, m
RNA, tRNA
14%
36%
7%
43%1. mRNA, tRNA, rRNA2. rRNA, tRNA, mRNA3. tRNA, mRNA, rRNA4. rRNA, mRNA, tRNA
In the primary structure of protein, the amino acids are linked together by what type of bond?
Disu
lfide bond
Hyd
rogen bond
Peptide bond
Ionic
bond
7% 7%
71%
14%
1. Disulfide bond2. Hydrogen bond3. Peptide bond4. Ionic bond
The atomic number of an atom is defined as its:
Mass
in AM
U
Number o
f elec..
.
Number o
f neut..
.
Number o
f pro
t...
0%
71%
14%14%
1. Mass in AMU2. Number of electrons3. Number of neutrons4. Number of protons
The sum of the atomic masses of all the atoms in a compound is called the:
Molecu
lar weig.
..
Empiri
cal fo
rm...
Molar v
olume
Percenta
ge co
m...
100%
0%0%0%
1. Molecular weight2. Empirical formula3. Molar volume4. Percentage
composition
Each of the following is a type of protein EXCEPT:
Stru
ctural
Enzy
me
Stero
id
Transport
0%7%
79%
14%
1. Structural2. Enzyme3. Steroid4. Transport
The cellular organelle that is of great value to geneticists because it carries only maternal DNA
is the:
Nucle
us
Golgi a
pparatu
...
Ribosome
Mito
chondrio
n
7%
79%
14%
0%
1. Nucleus2. Golgi apparatus3. Ribosome4. Mitochondrion
Your boss in the state crime lab gives you a sample of DNA obtained from the back side of a postage stamp. She asks you to do DNA fingerprinting on the sample in order to determine if it matches the DNA of a particular suspect. You recognize that you will
have to amplify the DNA sample since there is not enough to do the fingerprint analysis. What piece of equipment when you use to amplify the DNA
Centrifu
ge
Autocla
ve
Thermal C
ycler
Spectr
ophotome...
7%
29%
57%
7%
1. Centrifuge2. Autoclave3. Thermal Cycler4. Spectrophotometer
What is the name of the specific sequence of DNA that is cut by a restriction enzyme?
Palindro
me sequence
Start
codon se
quence
Recogniti
on sequence
TATA box sequence
0%7%
36%
57%1. Palindrome sequence
2. Start codon sequence
3. Recognition sequence
4. TATA box sequence
The study of the functions of specific proteins is known as:
Prote
omics
Genomics
Functi
onal syste
matics
Prote
inology
25% 25%25%25%1. Proteomics2. Genomics3. Functional
systematics4. Proteinology
You are preparing a buffer solution for your DNA experiment. The directions tell you to adjust the pH to 8.2. When you check the current pH, you observe that it is7.6. Which of the following solutions is the best choice for adding to
your buffer to adjust the pH to 8.2?
10% SDS
1M N
aOH
1M HCl
5M HCl
25% 25%25%25%1. 10% SDS2. 1M NaOH3. 1M HCl4. 5M HCl
Molecules in which the atoms share electrons equally would be called:
Acids
Bases
Hyd
rophobic
Hyd
rophilic
Polar
Non-P
olar
17% 17% 17%17%17%17%1. Acids2. Bases3. Hydrophobic4. Hydrophilic5. Polar6. Non-Polar
The best medium type for rapid, mass culturing of bacteria is:
Agar plat
e Li
quid St
ab Sl
ab
25% 25%25%25%1. Agar plate2. Liquid3. Stab4. Slab
What types of clothing are NOT appropriate in the lab?
Lab co
ats, gog..
.
Shorts
, sandal...
Jeans,
T-Shirt...
Blouse, S
kirt
25% 25%25%25%1. Lab coats, goggles2. Shorts, sandals3. Jeans, T-Shirts4. Blouse, Skirt
In preparing petri plates for a transformation experiment, you first measure out the correct amount of powered agar for the volume of water you have. Next, you will swirl the agar so that
most of it goes into solution (dissolves). Identify the equipment that you will use next.
Centrifu
ge
Thermal c
ycler
Spectr
ophotome...
Micr
owave
25% 25%25%25%1. Centrifuge2. Thermal cycler3. Spectrophotometer4. Microwave
One reason that prokaryotes have been such valuable model organisms in genetic studies is that their generation time is so short. In fact, some species can reproduce as often as every 20
minutes. This gives the researcher answers literally over night. With this in mind, which of the following eukaryotes would be the best model organism?
Human
Bread mold
Round worm
Yeast
25% 25%25%25%1. Human2. Bread mold3. Round worm4. Yeast
In order to make 3 liters of a .5M sucrose solution, how many grams of sucrose would you add. (The molecular weight of
sucrose is 342g)
1026g 513g
542g 171g
25% 25%25%25%1. 1026g2. 513g3. 542g4. 171g
DNA splits or unzips between the nitrogen bases of the DNA molecule. So as to expose the sense strand bases of the gene. This “unzipping” occurs because the bond between the bases is relatively weak and is
known as a _______ bond.
Ionic
Covalent
Hyd
rogen
Peptide
25% 25%25%25%1. Ionic2. Covalent3. Hydrogen4. Peptide
The basic building block of DNA is a(n):
Nucle
otide
Amino acid ATP
Deoxyri
bose
25% 25%25%25%1. Nucleotide2. Amino acid3. ATP4. Deoxyribose
When amplifying a sample of human DNA by PCR at a particular marker site, it is NOT absolutely critical that extraneous DNA, say from bacteria for sample, not contaminate the sample to be amplified. This is because:
The primers
used in
the...
The DNA polym
erase m
o...
Any contam
inant DNA ...
It is
statisti
cally
impro
ba...
25% 25%25%25%1. The primers used in the PCR reaction are unique to human DNA
2. The DNA polymerase molecule used in PCR can only copy human DNA sequences
3. Any contaminant DNA would immediately be degraded by enzymes present in the reaction mixture
4. It is statistically improbable that there would be enough contaminant DNA to serve as template DNA
Before turning the centrifuge machine on, you should always check to make sure:
Is it
plugged ...
The lid is
clo...
That the tu
bes...
That you have ...
25% 25%25%25%1. Is it plugged in2. The lid is closed3. That the tubes are
placed in balanced positions
4. That you have set the correct time
When should safety glasses be worn?
Durin
g any exp...
Any time you a...
Only w
hen work
...
Only w
hen work
...
25% 25%25%25%1. During any experiment with harmful chemicals.
2. Any time you are working in the lab on an experiment.
3. Only when working with bacteria, or glass.
4. Only when working with reagents, bacteria, or glass.
Gram negative bacteria stain ___ because of the ___ cell wall?
Red/thin
Purple/th
in
Red/thick
Purple/th
ick
25% 25%25%25%1. Red/thin2. Purple/thin3. Red/thick4. Purple/thick
How many moles of NaOH is in 120g of NaOH?
2 3 5120
25% 25%25%25%1. 22. 33. 54. 120
Proteins are synthesized (produced) in the
Ribosomes
Endoplasm
ic Reticu
lum
Lyso
somes
centri
oles
25% 25%25%25%1. Ribosomes2. Endoplasmic
Reticulum3. Lysosomes4. centrioles
The purpose of the antibiotic in a bacterial transformation experiment is to:
Kill any fo
rei...
Promote
the ba...
Preve
nt all b
u...
Promote
the gr..
.
25% 25%25%25%1. Kill any foreign microbes
2. Promote the bacteria to produce antibodies
3. Prevent all but the transformants from growing
4. Promote the growth of all but the transformants
Compounds that release hydrogen ions into solutions are called
Acids
Bases
Hyd
rophobic
Hyd
rophilic
25% 25%25%25%1. Acids2. Bases3. Hydrophobic4. Hydrophilic
The “magical” characteristic of Taq Polymerase is that it
Can reco
gnize ...
Is highly re
si...
Is highly re
si...
Maintai
ns its
...
25% 25%25%25%1. Can recognize cut sites from both directions
2. Is highly resistant to antibiotics such as ampicilin
3. Is highly resistant to mutagens such as radiation
4. Maintains its tertiary structure and ability to function under boiling conditions
5 mg is equivalent to
.005 ug
5 ug
500 ug
5,000 ug
25% 25%25%25%1. .005 ug2. 5 ug3. 500 ug4. 5,000 ug
The best way to obtain an accurate determination regarding the concentration of bacteria within a flask of liquid medium is to
Swirl
the m
edi...
Compare th
e de...
Run the bacte
r...
Obtai
n transm
i...
25% 25%25%25%1. Swirl the medium and make an approximation
2. Compare the density of the cultured medium with that of the uncultured medium
3. Run the bacterial DNA out in agarose gel electrophoresis
4. Obtain transmittance readings on the spectrophotometer
To streak bacteria on an agar plate, you would use a(n)
Micr
opipetter
Inocu
lating lo...
Pipette
Micr
opipette t...
25% 25%25%25%1. Micropipetter2. Inoculating loop3. Pipette4. Micropipette tip
Small circular loops of DNA (typically 3000 to 15,000 bp in length) naturally found in
bacteria are known as
Chromoso
mes
Genes
Plasmids
Chromatin
25% 25%25%25%1. Chromosomes2. Genes3. Plasmids4. Chromatin
The best way to measure out 0.157mL of a particular solution would be to use the ___ micropipetter with the dial set on ___ uL.
0.5 to 10/1
.57
2 to 20/1
5.7
20 to 200/1
57
100 to 1000/1
5...
25% 25%25%25%1. 0.5 to 10/1.572. 2 to 20/15.73. 20 to 200/1574. 100 to 1000/1570
In order to prevent contamination, it is absolutely essential to change pipet
tips, particularly
When m
ixing t
h...
Between aloquo...
Between aloquo...
When adding an...
25% 25%25%25%1. When mixing the ingredients within a sample
2. Between aloquots of the first ingredient added to the tubes of several samples
3. Between aloquots of the last ingredient added to the tubes of several samples
4. When adding antibiotics to growth media
Which term describes a molecule that is water-hating?
Hyd
rophobic
Polar
Hyd
rophilic
Ionic
25% 25%25%25%1. Hydrophobic2. Polar3. Hydrophilic4. Ionic
Which choice below lists the correct order of the steps performed during a
bacterial transformation?
Preincu
bation,...
Preincu
bation,...
Preincu
bation,...
Preincu
bation,...
25% 25%25%25%1. Preincubation, heat shock, incubation, recovery
2. Preincubation, incubation, heat shock, recovery
3. Preincubation, recovery, incubation, heat shock
4. Preincubation, heat shock, recovery, incubation
Microarray technology has recently become a tremendously valuable tool in the hands of
scientists because it reveals the
Loca
tion of ge...
Degre
e of “ide...
Degre
e to w
hic...
Base se
quence ...
25% 25%25%25%1. Location of genes upon chromosomes
2. Degree of “identicalness” between two samples of DNA of different sources
3. Degree to which several genes are individually expressed within a particular cell or tissue
4. Base sequence differences between diseased and normal genes
Regions of DNA that are transcribed into mRNA and then translated into
proteins are called?
Anticodons
Primers
Genes
Promote
rs
25% 25%25%25%1. Anticodons2. Primers3. Genes4. Promoters
Identify the statement that is FALSE with respect to the difference
between DNA and RNA.
DNA is
general...
DNA has t
hymin...
DNA has h
ydro
g...
DNA dire
ctly c.
..
25% 25%25%25%1. DNA is generally a double stranded, whereas RNA is generally single stranded.
2. DNA has thymine, whereas RNA has uracil.
3. DNA has hydrogen bonds, whereas RNA generally does not.
4. DNA directly codes for proteins, whereas RNA most often directly codes for DNA.
When drawing up a solution with a micropipetter, you should
Push th
e plung...
Put the tip in
...
Push th
e plung...
Put the tip in
...
25% 25%25%25%1. Push the plunger to the first stop before placing the tip in the solution and release the plunger slowly.
2. Put the tip in the solution first, then push the plunger to the first stop and release quickly.
3. Push the plunger to the second stop, put the tip in the solution then release the plunger quickly.
4. Put the tip in the solution first, then push the plunger to the second stop and release slowly.
The primary role of agar in plate growth media is to
Give
the m
edia a noncle..
Provide nutri
ents
Keep the m
edia from dr..
.
Give
the m
edia a gel con...
25% 25%25%25%1. Give the media a nonclear golden color
2. Provide nutrients3. Keep the media
from drying out4. Give the media a gel
consistency
What is the proper laboratory procedure when disposing of
microorganisms?
Place in
a clo...
Wra
p in a tr
as...
Expose
micr
oor...
Leave on th
e b...
25% 25%25%25%1. Place in a closed trash can.
2. Wrap in a trash bag and place in trash can.
3. Expose microorganisms to bleach or autoclave.
4. Leave on the bench top until they die.
In a serial dilution, the concentration of each successive sample is
Decre
ased by a...
Incre
ased by a...
Made to
remain...
Made to
remain...
25% 25%25%25%1. Decreased by a given fraction
2. Increased by a given fraction
3. Made to remain constant
4. Made to remain constant, then to become more dilute over time
Which method for transferring new DNA into bacteria involves the bacteria taking up pieces of DNA that are
floating freely in solution outside of the cell?
Conjugation
Transducti
on
Transform
ation...
33% 33%33%1. Conjugation2. Transduction3. Transformation
To date, the most valuable and widespread application of green fleurescent protein
(GFP) is
As a novelty
; ...
As a ta
g or ma...
As a tu
mor cel...
As an antigen ...
25% 25%25%25%1. As a novelty; green fleurescent things are really cool
2. As a tag or marker to indicate the presence of an accompanying introduced gene of interest
3. As a tumor cell probe revealing the presence of certain cancers
4. As an antigen to antibiotics such as ampicilin
The bond between the complimentary bases of a DNA molecule is
Ionic
Phosphoric
Covalent
Hyd
rogen
25% 25%25%25%1. Ionic2. Phosphoric3. Covalent4. Hydrogen