Work Package 6: Biomolecular motors on nanostructures

Midterm-Review Meeting

Molecular Machines- Design and Nano-Scale Handling of Biological Antetypes and Artificial Mimics

Specific Targeted Research Project (STREP) in FP6-NMP-2002

Objectives

Partners

PM

Construct kinesin-motor based biological-inorganic hybrid devices for controlled nano-transport processes.

Construct kinesin-motor based biological-inorganic hybrid devices for controlled nano-transport processes.

ICG-GC TU/d-MB EPFL ETH

30 30 24 6

Midterm-Review Meeting

The molecular motors Work Package 6: Biomolecular motors on nanostructures

Cytoskeleton motors : Kinesin, Myosin...Cytoskeleton motors : Kinesin, Myosin...

Microtubules Kinesin

f-actin Myosin

How do they work ?Energy conversion

(M6-1) Structural changes vs. ATP hydrolysisHigh resolution techniques

How include the motors in nano-devices ?

(D6-1) Hybrid organic/inorganic systemsNano-handling

APD

DetectionDetection

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Traveling Wave Tracking Work Package 6: Biomolecular motors on nanostructures

Single 10 nm step

RMS noise ± 2 Å

Piezo: 10 nanometers steps

Time resolution ~ 2 µs

RMS noise ~ 2 Å

Drift ~ 1 nm/s

Midterm-Review Meeting

Traveling Wave Tracking Work Package 6: Biomolecular motors on nanostructures

- +Many motors ?

- +Single motor ?

T = 300 K

x = 7.4 pN s/nm

x = 0.3 pN/nm

Average speed = 500 nm/s

Backward steps ~ 10 %

Step time scale < 70 µs

Midterm-Review Meeting

Work Package 6: Biomolecular motors on nanostructures

Midterm-Review Meeting

Structures designed with a 3D approach for the TWT setup (collaboration with the Institut Curie)

Glass

3D approach

Some samples

Work Package 6: Biomolecular motors on nanostructures

In order to observe 3D movements, including rotations, the object of study has to be suspended

Soft Lithography approach

Midterm-Review Meeting

Work Package 6: Biomolecular motors on nanostructures

Nanostructures for kinesin-driven microtubule motility

Rectification, confinement and electrical docking

Time sequence of microtubule

docking

Midterm-Review Meeting

Rectification of kinesin-driven microtubule motility

SEM image of rectifying structure

Fluorescence image of microtubule motility in nanostructures

Rectifier geometries

92 % efficiency in rectification

Work Package 6: Biomolecular motors on nanostructures

Midterm-Review Meeting

Specific Targeted Research Project (STREP) in FP6-NMP-2002

Work Package 6: Biomolecular motors on nanostructures

Production Van den Heuvel, M. G. L.; Butcher, C. T.; Lemay, S. G.; Diez, S.; Dekker, C. Nano Lett. 2005, 5, 235-241.Van den Heuvel, M. G. L.; Butcher, C. T.; Smeets, R. M. M.; Diez, S.; Dekker, C. Nano Lett. 2005, 5, 1117-1122.L. Busoni, A Dornier, J-L Viovy, J. Prost, G. Cappello; Journal of Applied Physics 2005, 98 A. Dupont, C. Symonds, L. Busoni, J. Prost, G. Cappello; In preparation (2005)

M6-1 M6-2 and D6-1

Many different nanofabricated structures were made in TU-Delft New flexible optical technique, called Travelling Wave Tracking (ICG-GC). Microtubules injected in the structures (TU-Delft) and the activity has successfully been checkedFirst experiments on single molecular motor

Resources Post-Docs: Irene Dujovne, Clémentine Symonds, Lorenzo BusoniConferences and Meetings

additionalprojects

New motors: Myosin V and VI (collaboration with J. Spudich, Stanford)Gold nanoprobes (collaboration with R. Levy, University of Liverpool)DNA associated motors

Conclusion Objectives for the first part of the project have been achieved and we can begin measuring single molecular motors moving on the suspended microtubules.