work process and sop for taking blood culture

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Work Process : Taking Blood Culture Label the Blood Culture bottle and fill up the lab form including time the specimen is Prepare trolley and Blood C&S set Disinfect the skin area 3 times with Hibitane 2% ( 1:50) in 70% Alcohol. Do not palpate the vein after the skin is disinfected. Leave it to dry Apply tourniquet Drape the area with a sterile towel. Perform the venipuncture using a minimum of 10 ml syringe. Change injection needle if re-venipuncture required. Send the Blood Culture specimen to the lab. as soon as possible Invert the Blood Culture bottle gently 5 to 10 times to prevent clotting End Start Wear mask/ Perform handwashing then wear sterile glove Change the injection needle. Swab the Blood Culture bottle with Hibitane 2% (1:50) Inoculate blood into the Blood Culture bottle. Identify patient. Blood Culture should be taken and then start on i/v antibiotics within 1 hour of

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Page 1: Work Process and SOP for Taking Blood Culture

Work Process : Taking Blood Culture

PROSES KERJA: TAKING BLOOD CULTURE

NO. TINDAKAN TANGGUNGJAWAB

Label the Blood Culture bottle and fill up the lab form including time the specimen is takenPrepare trolley and Blood C&S set

Disinfect the skin area 3 times with Hibitane 2% ( 1:50) in 70% Alcohol. Do not palpate the vein after the skin is disinfected. Leave it to dry (about 15 seconds to 1minute).Apply tourniquet

Drape the area with a sterile towel.

Perform the venipuncture using a minimum of 10 ml syringe. Change injection needle if re-venipuncture required.Change injection needle if re-venipuncture required.

Send the Blood Culture specimen to the lab. as soon as possible

Invert the Blood Culture bottle gently 5 to 10 times to prevent clotting

End

Start

Wear mask/ Perform handwashing then wear sterile glove

Change the injection needle.Swab the Blood Culture bottle with Hibitane 2% (1:50) in 70% Alcohol.

Inoculate blood into the Blood Culture bottle.

Identify patient. Blood Culture should be taken and then start on i/v antibiotics within 1 hour of recognition of sepsis.

Page 2: Work Process and SOP for Taking Blood Culture

1. Identify patient. Blood Culture should be taken and then start on i/v antibiotics within 1 hour of recognition of sepsis.

Pegawai Perubatan/Jururawat Terlatih

2. Label the Blood Culture bottle and fill up the lab form including time the specimen is taken

Pegawai Perubatan/Jururawat Terlatih

3. Prepare trolley and Blood C&S set Pegawai Perubatan/ Jururawat Terlatih

4. Wear mask/ Perform handwashing then wear sterile glove

Pegawai Perubatan

5. Disinfect the skin area 3 times with Hibitane 2% (1:50) in 70% Alcohol. Do not palpate the vein after the skin is disinfectedLeave it to dry (about 15 seconds to 1 minute).

Pegawai Perubatan

6. Apply tourniquet Jururawat Terlatih

7. Drape the area with a sterile towel. Pegawai Perubatan

8. Perform the venipuncture using a minimum of 10 ml syringe.Change injection needle if re-venipuncture required.

Pegawai Perubatan

9. Change the injection needle.Swab the Blood Culture bottle with Hibitane 2% (1:50) in 70% Alcohol.

Pegawai Perubatan

10. Inoculate blood into the Blood Culture bottle. Pegawai Perubatan

11. Invert the Blood Culture bottle gently 5 to 10 times to prevent clotting

Pegawai Perubatan

12. Send the Blood Culture specimen to the lab - as soon as possible

Penolong Perubatan Kesihatan

SENARAI SEMAK : TAKING BLOOD CULTURE

Page 3: Work Process and SOP for Taking Blood Culture

TINDAKAN TANDA ( √ )

Identify patient. Blood Culture should be taken and thenstart on i/v antibiotics within 1 hour of recognition of sepsis.

Label the Blood Culture bottle and fill up the lab form including time the specimen is taken

Prepare trolley and Blood C&S set

Wear mask/ Perform handwashing then wear sterile glove Disinfect the skin area 3 times with Hibitane 2% (1:50) in 70% Alcohol. Do not palpate the vein after the skin is disinfected Leave it to dry (about 15 seconds to 1 minute). Apply tourniquet

Drape the area with a sterile towel.

Perform the venipuncture using a minimum of 10 ml syringe.Change injection needle if re-venipuncture required.

Change the injection needle.Swab the Blood Culture bottle with Hibitane 2% (1:50) in 70% Alcohol. Inoculate blood into the Blood Culture bottle.

Invert the Blood Culture bottle gently 5 to 10 times to prevent clotting

Send the Blood Culture specimen to the lab - as soon as possible

Page 4: Work Process and SOP for Taking Blood Culture

NORMA KERJATUGAS : TAKING BLOOD CULTURE

PROSES KERJA MASA YANG DIAMBIL

1. Identify patient. Blood Culture should be taken and then start on i/v antibiotics within 1 hour of recognition of sepsis. 1 Minit

2. Label the Blood Culture bottle and fill up the lab form including time the specimen is taken

5 Minit

3. Prepare trolley and Blood C&S set5 Minit

4. Wear mask/ Perform handwashing then wear sterile glove 5 Minit

5. Disinfect the skin area 3 times with Hibitane 2% ( 1:50) in 70% Alcohol. Do not palpate the vein after the skin is disinfectedLeave it to dry (about 15 seconds to 1 minute).

5 Minit

6. Apply tourniquet1 Minit

7. Drape the area with a sterile towel.1 Minit

8. Perform the venipuncture using a minimum of 10 ml syringe.Change injection needle if re-venipuncture required.

2 Minit

9. Change the injection needle.Swab the Blood Culture bottle with Hibitane 2% (1:50) in 70% Alcohol.

1 Minit

10. Inoculate blood into the Blood Culture bottle.1 Minit

11. Invert the Blood Culture bottle gently 5 to 10 times to prevent clotting 1 Minit

12. Send the Blood Culture specimen to the lab - as soon as possible 10 Minit

Jumlah 38 Minit

Page 5: Work Process and SOP for Taking Blood Culture