wp5: genetic erosion and genetic pollution methodologies
TRANSCRIPT
WP5: Genetic Erosion and Genetic Pollution
Methodologies
Overall objectives
• Assessment of methodologies for
monitoring actual genetic erosion and
pollution
• Assessment of methodologies for
prediction of likely genetic erosion and
pollution in the future
Possible strategy for discussion
Identify accessions of wild species relatives that have been in storage without seed increase, for 20/30/40 years: with accurate passport information
Revisit collection sites, and where populations still exist, re-record passport data and sample seeds
Where populations no longer exist, collect from adjacent comparable sites
Identify control samples of germplasm in gene banks where similar sets of accessions have been in storage (without rejuvenation) for different periods of time (eg 10, 20, 30 years)
Undertake high throughput genotyping
Undertake analysis of population genetic parameters to determine genetic erosion
Discussion points for WP5 - I. MATERIAL • Which species and how many can we
realistically study?
• Over what time scale?
• Does suitable material exist in genebanks?
• Do suitable complementary populations still exist in the wild?
• Is it possible to identify populations vulnerable to pollution by modern/GM varieties?
Discussion points for WP5 - II. CAUSES OF EROSION
• Greater appreciation of some or all of the
underlying causes of loss?
• How to assess causes of erosion other than
by taking a few specific examples?
• How can we use ecogeography?
Discussion points for WP5 - III. MEASURING EROSION• How do we actually measure erosion?
• How reliable/convincing will population genetic parameters be?
• Estimators of – gene flow? – population differentiation?– diversity?– distribution of molecular variance?
Discussion points for WP5 - IV. MEASURING POLLUTION
• Direct methods?
• Available markers?
• Indirect methods?
Discussion points for WP5 - V. MOLECULAR MARKERS
• Need for high throughput?
• Which marker system(s) to choose?
• Do we need neutral markers?
• Do we need markers that will reflect loss of
adaptive variation?
Discussion points for WP5 - VI. NEW IDEAS
• Any other methods or approaches available?
• To form part of major discussion
Discussion points for WP5 - VII. GUEST SPEAKERS
• We can invite two or three key experts to
advise on these issues - who?
• Molecular marker expert - GENE MINE?
• Population geneticist (gene flow/population
structure)?
• GM/gene flow and genetic pollution?
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