x™ provirus quantitation kit - scientifix promo march 18 2014 v4... · fast/accurate titration...

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Code Description Size List Price Promo Price 631235 Lenti-X™ qRT-PCR 200 Rxns $ 759 $ 645 + GST Titration Kit Code Description Size List Price Promo Price 631239 Lenti-X™ Provirus 200 $ 759 $ 645 + GST Quantitation Kit Rxns Get a YES or NO in only 30 seconds Know when to harvest Lentiviral supernatants Code Description Size List Price Promo Price 631243 Lenti-X™ GoStix™ 20 Tests 573 $ $ 487 + GST 631244 Lenti-X™ GoStix™ 50 Tests 1,236 $ 1,050 + GST Fast/easy ELISA-based titration protocol Correlate p24 capsid protein content to virus Code Description Size List Price Promo Price 632200 Lenti-X™ p24 96 Rxns 899 $ $ 764 + GST Rapid Titer Kit Fast/accurate titration using SYBR® Green Harvest, titer, and infect in one day Obtain results in 4 hours Measure copy number of integrated lentivirus (provirus) using a SYBR® Green-based qPCR Compare transduction data across multiple cell lines http://info.clontech.com/Tools_Titrationkits.html http://info.clontech.com/Tools_Concentrationa ndPurificationkits.html Increase titer without ultracentrifugation Scalable to any volume or titer 100X concentration with 90% recovery Code Description Size List Price Promo Price 631231 Lenti-X™ Concentrator 100 mL 372 $ $ 316 + GST 631232 Lenti-X™ Concentrator 500 mL 1,311 $ $ 1,114 + GST High yield and purity Gentle gravity column-based protocol Preserves lentiviral particle integrity Concentrates ultrapure virus up to 10-fold Code Description Size List Price Promo Price 631233 Lenti-X™ Maxi Purification Kit 2 Preps 429 $ $ 365 + GST 631234 Lenti-X™ Maxi Purification Kit 5 Preps 820 $ $ 697 + GST 631245 Lenti-X™ Maxi Purification Kit* Each 891 $ $ 757 + GST 631246 Collection Rack L Each 142 $ $121 + GST *631245 includes rack

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Code Description Size List Price Promo Price 631235 Lenti-X™ qRT-PCR 200 Rxns $ 759 $ 645 + GST

Titration Kit

Code Description Size List Price Promo Price 631239 Lenti-X™ Provirus 200 $ 759 $ 645 + GST

Quantitation Kit Rxns

Get a YES or NO in only 30 seconds

Know when to harvest Lentiviral supernatants

Code Description Size List Price Promo Price

631243 Lenti-X™ GoStix™ 20 Tests 573$ $ 487 + GST

631244 Lenti-X™ GoStix™ 50 Tests 1,236$ 1,050 + GST

Fast/easy ELISA-based titration protocol Correlate p24 capsid protein content to

virus

Code Description Size List Price Promo Price

632200 Lenti-X™ p24 96 Rxns 899$ $ 764 + GST

Rapid Titer Kit

Fast/accurate titration using SYBR® Green

Harvest, titer, and infect in one day

Obtain results in 4 hours

Measure copy number of integrated lentivirus (provirus) using a SYBR® Green-based qPCR

Compare transduction data across multiple cell lines

http://info.clontech.com/Tools_Titrationkits.html

http://info.clontech.com/Tools_Concentrationa

ndPurificationkits.html

Increase titer without ultracentrifugation

Scalable to any volume or titer 100X concentration with 90% recovery

Code Description Size List Price Promo Price

631231 Lenti-X™ Concentrator 100 mL 372$ $ 316 + GST

631232 Lenti-X™ Concentrator 500 mL 1,311$ $ 1,114 + GST

High yield and purity Gentle gravity column-based protocol Preserves lentiviral particle integrity

Concentrates ultrapure virus up to 10-fold

Code Description Size List Price Promo Price

631233 Lenti-X™ Maxi Purification Kit 2 Preps 429$ $ 365 + GST

631234 Lenti-X™ Maxi Purification Kit 5 Preps 820$ $ 697 + GST

631245 Lenti-X™ Maxi Purification Kit* Each 891$ $ 757 + GST

631246 Collection Rack L Each 142$ $121 + GST

*631245 includes rack

http://www.clontech.com/US/Products/Viral_

Transduction/Lentiviral_Vector_Systems/Selec

tion_Guide

Express your gene of interest from a strong CMV promoter, and

select for lentiviral integration using antibiotic selection

Co-express your gene of interest and an antibiotic or fluorescent selectable marker from the same transcript using

these IRES-containing lentiviral vector systems.

Express your gene of interest from an EF-1 alpha promoter in cell types where the CMV promoter can be silenced over time,

such as hematopoietic or stem cells.

Obtain tightly controlled, inducible expression clones of your gene of interest with only a single vector.

Clone your shRNA downstream of a U6 promoter to knock down gene expression in hard to transfect cells.

Clone your gene downstream of a ProteoTuner destabilization domain (DD) and control the stability of your protein with Shield1, a small molecule ligand, which protects

the DD fusion protein from being degraded.

Monitor activity of your favourite promoter using a reporter system that expresses bright fluorescent proteins or secreted luciferase.

Code Description Size List Price Promo Price

T100A RetroNectin® Recombinant 0.5 mg $285 $ 242 +GST

Human Fibronectin Fragment

T100B RetroNectin® Recombinant 2.5 mg $1,107 $ 941 + GST

Human Fibronectin Fragment

T110A RetroNectin® Precoated Dish 10

Dishes $568 $ 483 + GST

T201 RetroNectin® (GMP) 2.5 mg $2,402 $ 2,042 + GST

Clone your gene in-frame with one of Clontech’s many fluorescent proteins (FP) and monitor the expression and

subcellular localization of your protein of interest.

Monitor activity of your favourite promoter using a reporter system that expresses a cyan, green or red fluorescent protein on demand.

Fast and highly efficient transduction in 5min

Magnetic bead-based—no polybrene required

For lentivirus & MMLV retrovirus

Ideal for sensitive cell types (e.g. stem cells)

Transduce any cell type with ecotropic lentivirus including human cells

Increase transduction efficiency by delivering mCAT-1 viral receptor protein to your target cell

http://info.clontech.com/Tools_Transductionenha

ncers.html

Multivalent molecule simultaneously binds virus and cell surface proteins

Widely used for hematopoietic cells and stem cells

Increased transduction of hard-to-infect cells

Nontoxic: does not require cytotoxic polybrene

GMP option for ex vivo clinical applications

Code Description Size List Price Promo Price

631254 Lenti-X™ Accel. Starter Kit Each 1,528$ $ 1,299 + GST

631256 Lenti-X™ Accel.rator 400 uL 627$ $ 533 + GST

631257 Lenti-X™ Accelerator 1,000 uL 1,254$ $ 1,065.9 + GST

Code Description Size List Price Promo Price

631471 Ecotropic Receptor 20 Rxns 470$ $ 400 +GST

Booster

Code Description Size List price Promo Price

631258 Lenti-X™ HTX Packaging 20 Rxns 1,679$ $ 1,427 +GST

System (Integrase Deficient)

Generates 25x more lentivirus

Obtain >108 IFU/ml from one 10 cm plate

Very high safety profile, (4th Generation) Compatible with any lentiviral vector system

VSV-G pseudotyped lentivirus, which infects virtually all types of cells

Create lentiviral supernatants that have ecotropic envelopes

produce lentivirus pseudotyped with the MLV ecotropic envelope glycoprotein

limit transduction to mouse and rat cells.

Gene delivery without insertion into the host genome

Reduced risk of insertional mutagenesis

VSV-G pseudotype

Use with any lentiviral vector to generate non-integrative lentivirus

http://www.clontech.com/US/Products/Vir

al_Transduction/Lentiviral_Packaging/High

-Titer_Lentivirus

Scientifix Pty Ltd.

Promotional pricing valid until June 30, 2014*

Quote: LENTIXQ214PROMO

*Freight charges apply. Please contact Scientifix for details

*Prices subject to change without notice

FREECALL: 1800 007 900

[email protected]

www.scientifix.com.au