xba i 19 7.7 6.2 4.3 kbp sac i hind iii fig. s1 southern blot analysis using genomic dna from nona...
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DESCRIPTION
Vector No-OsHKT2;2/1 EGFP-No-OsHKT2;2/1 1.0K 0.1K Fig. S3 Complementation test using high-affinity K + uptake deficient mutant (strain CY162) of S. cerevisiae. CY162 cells harboring either vector (pYES2) or pYES2::No-OsHKT2;2/1 or pYES2::EGFP-No-OsHKT2;2/1 were grown on AP medium containing 1.0 mM (left) or 0.1 mM (right) KCl. 1:10 serial dilutions of each transformant were spotted on the plates with the starting OD 600 of 0.1. All plates were incubated at 30 ºC for 4d and pictures were taken afterwards