Xray crystallography

Lasse Mårtensson, IFM-Chemistry

Ribosome

• © 2006 • Academic Press

• © 2006 • Academic Press

Diffraction pattern from a protein obtained by xray crystallography

• © 2006 • Academic Press

Electrondensity map

• © 2006 • Academic Press

• © 2006 • Academic Press

• © 2006 • Academic Press

• © 2006 • Academic Press

• © 2006 • Academic Press

•  Requirements: •  Pure protein of high concentration 10-50

mg/ml AND… •  Protein crystals How to obtain protein crystals??

• © 2006 • Academic Press

• © 2006 • Academic Press

• © 2006 • Academic Press

• © 2006 • Academic Press

• © 2006 • Academic Press

Crystallization robot

Terms used in Xray crystallography

•  Crystal system •  Spacegroup •  Unit cell •  Assymmetric unit •  Miller indices hkl •  Braggs law •  Ewald sphere