NO

YES

Import sequences of targeted phylum from 16S rRNA ribosomal databases

Extract the N most representative sequences of the phylum

Input the N sequences to the motif search program MEME Identification of n patterns

Test each of the n patterns against the 16S rDNA sequences of the databases

Select pattern as a potential barcode is dependent onsensitivity and specificity thresholds

Generate the DNA barcodeBest scores for sensitivity and specificity

Patterns are over ?

-Maximum Hits in the phylum (Sensitivity)

-Minimum Hits in the rest of Bacteria Domain (Specificity)