yesterday: genetic disorders and gene therapy today: genetic engineering

12
Yesterday: Yesterday: Genetic Disorders Genetic Disorders and and Gene Therapy Gene Therapy Today: Today: Genetic Engineering Genetic Engineering

Upload: amos-alexander

Post on 29-Dec-2015

219 views

Category:

Documents


2 download

TRANSCRIPT

Page 1: Yesterday: Genetic Disorders and Gene Therapy Today: Genetic Engineering

Yesterday:Yesterday:Genetic Disorders Genetic Disorders

and and Gene TherapyGene Therapy

Today:Today:Genetic EngineeringGenetic Engineering

Page 2: Yesterday: Genetic Disorders and Gene Therapy Today: Genetic Engineering

What does Genetic Engineering mean to You?

Page 3: Yesterday: Genetic Disorders and Gene Therapy Today: Genetic Engineering

Overview of Genetic Engineering

• What are the differences between cloning, recombinant DNA, and genetic engineering?

• What are the tools we use for genetic engineering?– Plasmids– Restriction Enzymes– DNA Ligase

Page 4: Yesterday: Genetic Disorders and Gene Therapy Today: Genetic Engineering

Cloning Involves Making Identical Copies

“Cloning” can mean several things:– To make many identical copies of a

DNA molecule or a particular stretch of DNA (DNA cloning or molecular cloning).

– To replicate an entire organism (reproductive cloning).

Page 5: Yesterday: Genetic Disorders and Gene Therapy Today: Genetic Engineering

Recombinant DNA

• Recombinant DNA is when two or more pieces of DNA that don’t normally go together are combinedcombined.

Page 6: Yesterday: Genetic Disorders and Gene Therapy Today: Genetic Engineering

Genetic Engineering

• Genetic Engineering is the process of using recombinant DNA technology to genetically alter or create new organisms.

Page 7: Yesterday: Genetic Disorders and Gene Therapy Today: Genetic Engineering

Recombinant DNA Technology Tools

• Plasmid Vectors– To move DNA from one organism to

another.

• Restriction Enzymes– “Molecular Scissors” to cut DNA in

specific places.

• DNA Ligase– DNA “glue” to stick pieces of DNA

together.

Page 8: Yesterday: Genetic Disorders and Gene Therapy Today: Genetic Engineering

PlasmidsSmall, circular pieces of “extra” DNA found in bacteria.

Plasmids often carryantibiotic resistance.

Plasmids

Page 9: Yesterday: Genetic Disorders and Gene Therapy Today: Genetic Engineering

Restriction Enzymes:Molecular Scissors

A restriction enzyme (RE) is a specialized protein that cuts DNA in a very specific place.

Different REs cut at different places along the nucleotide sequence.

Page 10: Yesterday: Genetic Disorders and Gene Therapy Today: Genetic Engineering

Cell DNA

DNA Ligase (Glue)

Recombinant DNA

DNA fragment ligated (inserted) into vector creating recombinant DNA molecule.

Page 11: Yesterday: Genetic Disorders and Gene Therapy Today: Genetic Engineering

Today• Gene Therapy Vector Engineering

Project.– Use paper models to simulate the

process of engineering your recombinant gene therapy vector.

• DNA sheet for the therapeutic gene to treat the genetic disease you were assigned.

• DNA sheet for the vector you chose to treat the disease.

Page 12: Yesterday: Genetic Disorders and Gene Therapy Today: Genetic Engineering

Materials

• In lab folder (one per group):– Activity Instruction Sheet (WHITE)– Answer/Data Sheet (WHITE)– Restriction Enzyme Sheet (GREEN)

• Get from me (one per group):– Vector DNA sheet (PINK)– Therapeutic DNA sheet (BLUE)

• Other: scissors/glue stick