Yin-Yang Column for Global Yin-Yang Column for Global Protein IdentificationProtein Identification

Column Technology Inc.Column Technology Inc.

Yin-Yang MDLC-MS/MS

The Yin-Yang column combines anion and cation exchange chromatography For the protein identification through multi-LC/MS/MS:

Advantages of Yin-yang MDLC-MS/MSAdvantages of Yin-yang MDLC-MS/MS

► Three-dimensional LC-MS/MSThree-dimensional LC-MS/MS

► SCX and ANX are complimentary methods for peptide fractionationSCX and ANX are complimentary methods for peptide fractionation

► Most complete coverage of peptides and proteins Most complete coverage of peptides and proteins

► Enrichment and high-sensitivity for phosphopeptidesEnrichment and high-sensitivity for phosphopeptides

► Identify phospho and non-phospho peptides in the same run.Identify phospho and non-phospho peptides in the same run.

0 626

15604

27321544

42102308

123 656

3552

0 181233

10213

60297935

5050

9396

370

2359

12357

19100

0

5000

10000

15000

20000

25000

<3. 0 3. 0- -3. 5

3. 5- -4. 0

4. 0- -4. 5

4. 5- -5. 0

5. 0- -5. 5

5. 5- -6. 0

6. 0- -7. 0

7. 0- -8. 0

8. 0- -8. 5

>=8. 5

ML_CA_SAX

ML_CA_SCX

Theoretical pI distribution of identified non-phosphrylated peptides

Complementary peptide identification of SCX and SAX

90943047

10254

SAX SCX

108413 Unique peptides

6184 Proteins

SAX SCX2040

4072 4152

only 1mg protein digests of mouse liver, 5% FPRAnother More than 1000 phosphopeptides identified