yong huang department of mechanical engineering clemson university, clemson, sc scaffold-free...
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![Page 1: Yong Huang Department of Mechanical Engineering Clemson University, Clemson, SC Scaffold-free Alginate Tube Fabrication using Inkjet and Laser printing](https://reader036.vdocument.in/reader036/viewer/2022062314/56649e4e5503460f94b45807/html5/thumbnails/1.jpg)
Yong HuangDepartment of Mechanical
Engineering
Clemson University, Clemson, SC
Scaffold-free Alginate Tube Fabrication using Inkjet and Laser printing
![Page 2: Yong Huang Department of Mechanical Engineering Clemson University, Clemson, SC Scaffold-free Alginate Tube Fabrication using Inkjet and Laser printing](https://reader036.vdocument.in/reader036/viewer/2022062314/56649e4e5503460f94b45807/html5/thumbnails/2.jpg)
Table of Contents Introduction Background Objective Research Tasks
Tube Inkjetting Tube Laser Printing
Conclusions Future Work
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![Page 3: Yong Huang Department of Mechanical Engineering Clemson University, Clemson, SC Scaffold-free Alginate Tube Fabrication using Inkjet and Laser printing](https://reader036.vdocument.in/reader036/viewer/2022062314/56649e4e5503460f94b45807/html5/thumbnails/3.jpg)
Table of Contents Introduction Background Objective Research Tasks
Tube Inkjetting Tube Laser Printing
Conclusions Future Work
3
![Page 4: Yong Huang Department of Mechanical Engineering Clemson University, Clemson, SC Scaffold-free Alginate Tube Fabrication using Inkjet and Laser printing](https://reader036.vdocument.in/reader036/viewer/2022062314/56649e4e5503460f94b45807/html5/thumbnails/4.jpg)
Background Inkjet-based
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[Boland, 2007]
Inkjetting of CaCl2 into 2% alginic acid to make a branched structure
[Nakamura, 2008]
Inkjetting 0.8% sodium alginate into
2% CaCl2 to make a tube with the help of gravity
![Page 5: Yong Huang Department of Mechanical Engineering Clemson University, Clemson, SC Scaffold-free Alginate Tube Fabrication using Inkjet and Laser printing](https://reader036.vdocument.in/reader036/viewer/2022062314/56649e4e5503460f94b45807/html5/thumbnails/5.jpg)
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[Skardal, 2010]
Printing of agarose and 3T3 cell-containing PEGTA filaments to make a tube
[Norotte, 2009]
Printing of agarose and smooth muscle cell (SMC) cylinders to make a tube
Background Filament-based
![Page 6: Yong Huang Department of Mechanical Engineering Clemson University, Clemson, SC Scaffold-free Alginate Tube Fabrication using Inkjet and Laser printing](https://reader036.vdocument.in/reader036/viewer/2022062314/56649e4e5503460f94b45807/html5/thumbnails/6.jpg)
Background Laser-based
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Laser printing of alternating layers of fibroblasts (green) and keratinocytes (red) to form a grid structure (Scale bar = 500 µm)
[Koch, 2012]
![Page 7: Yong Huang Department of Mechanical Engineering Clemson University, Clemson, SC Scaffold-free Alginate Tube Fabrication using Inkjet and Laser printing](https://reader036.vdocument.in/reader036/viewer/2022062314/56649e4e5503460f94b45807/html5/thumbnails/7.jpg)
Challenges
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How to scale-up scaffold-free bioprint complex structures such as overhang structures?
How to evaluate and mitigate the post-printing cell damage?
![Page 8: Yong Huang Department of Mechanical Engineering Clemson University, Clemson, SC Scaffold-free Alginate Tube Fabrication using Inkjet and Laser printing](https://reader036.vdocument.in/reader036/viewer/2022062314/56649e4e5503460f94b45807/html5/thumbnails/8.jpg)
Table of Contents Introduction Background Objective Research Tasks
Tube Inkjetting Tube Laser Printing
Conclusions Future Work
8
![Page 9: Yong Huang Department of Mechanical Engineering Clemson University, Clemson, SC Scaffold-free Alginate Tube Fabrication using Inkjet and Laser printing](https://reader036.vdocument.in/reader036/viewer/2022062314/56649e4e5503460f94b45807/html5/thumbnails/9.jpg)
Objective
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Needs in cellular tube bioprintingVascularization as a main technological barrier for building
3D organs
Tube printing - a logical initial step towards vascularization
Objective
To scaffold-free fabricate viable cellular tubes using inkjet and laser printing
![Page 10: Yong Huang Department of Mechanical Engineering Clemson University, Clemson, SC Scaffold-free Alginate Tube Fabrication using Inkjet and Laser printing](https://reader036.vdocument.in/reader036/viewer/2022062314/56649e4e5503460f94b45807/html5/thumbnails/10.jpg)
Table of Contents Introduction Background Objective Research Tasks
Tube Inkjetting Tube Laser Printing
Conclusions Future Work
10
![Page 11: Yong Huang Department of Mechanical Engineering Clemson University, Clemson, SC Scaffold-free Alginate Tube Fabrication using Inkjet and Laser printing](https://reader036.vdocument.in/reader036/viewer/2022062314/56649e4e5503460f94b45807/html5/thumbnails/11.jpg)
Table of Contents Introduction Background Objective Research Tasks
Tube Inkjetting Tube Laser Printing
Conclusions Future Work
11
![Page 12: Yong Huang Department of Mechanical Engineering Clemson University, Clemson, SC Scaffold-free Alginate Tube Fabrication using Inkjet and Laser printing](https://reader036.vdocument.in/reader036/viewer/2022062314/56649e4e5503460f94b45807/html5/thumbnails/12.jpg)
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Liquid levelFirst layerSecond layer
3T3 cell suspension
Tube Inkjetting Experimental setup
![Page 13: Yong Huang Department of Mechanical Engineering Clemson University, Clemson, SC Scaffold-free Alginate Tube Fabrication using Inkjet and Laser printing](https://reader036.vdocument.in/reader036/viewer/2022062314/56649e4e5503460f94b45807/html5/thumbnails/13.jpg)
Tube Inkjetting - Zig-zag tube
Fabrication process: bottom part, overhang part and top part
Wall thickness: 150~200 µm
Tube diameter: 3 mm and length: 10 mm
Zigzag tube fabrication
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![Page 14: Yong Huang Department of Mechanical Engineering Clemson University, Clemson, SC Scaffold-free Alginate Tube Fabrication using Inkjet and Laser printing](https://reader036.vdocument.in/reader036/viewer/2022062314/56649e4e5503460f94b45807/html5/thumbnails/14.jpg)
Tube Inkjetting - Cell viability test
(a) Printed tube, (b) tube surface and (c) cell viability test after liquefying
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(a)
Cells
(b)
Live Cell
Dead Cell
After liquefying
(c)
![Page 15: Yong Huang Department of Mechanical Engineering Clemson University, Clemson, SC Scaffold-free Alginate Tube Fabrication using Inkjet and Laser printing](https://reader036.vdocument.in/reader036/viewer/2022062314/56649e4e5503460f94b45807/html5/thumbnails/15.jpg)
Cell viability immediately after printing: > 85% Cell viability after 3 days: > 80% Cell viability comparable to that of the control
Tube Inkjetting - Cell viability
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0 24 48 7270
75
80
85
90
95
Cel
l via
bilit
y %
Post-printingControl
(Hours)
![Page 16: Yong Huang Department of Mechanical Engineering Clemson University, Clemson, SC Scaffold-free Alginate Tube Fabrication using Inkjet and Laser printing](https://reader036.vdocument.in/reader036/viewer/2022062314/56649e4e5503460f94b45807/html5/thumbnails/16.jpg)
Tube Inkjetting - Other structures
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Pyramid Cone
![Page 17: Yong Huang Department of Mechanical Engineering Clemson University, Clemson, SC Scaffold-free Alginate Tube Fabrication using Inkjet and Laser printing](https://reader036.vdocument.in/reader036/viewer/2022062314/56649e4e5503460f94b45807/html5/thumbnails/17.jpg)
Table of Contents Introduction Background Objective Research Tasks
Tube Inkjetting Tube Laser Printing
Conclusions Future Work
17
![Page 18: Yong Huang Department of Mechanical Engineering Clemson University, Clemson, SC Scaffold-free Alginate Tube Fabrication using Inkjet and Laser printing](https://reader036.vdocument.in/reader036/viewer/2022062314/56649e4e5503460f94b45807/html5/thumbnails/18.jpg)
Tube laser printing - Experimental setup
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ArF laser
Pulsed laser beam
Ribbon
Forming jet/droplet
Quartz support
Coating
Container
Optical table
Laser-induced vapor/plasma
3-axis stages
Moving platform
![Page 19: Yong Huang Department of Mechanical Engineering Clemson University, Clemson, SC Scaffold-free Alginate Tube Fabrication using Inkjet and Laser printing](https://reader036.vdocument.in/reader036/viewer/2022062314/56649e4e5503460f94b45807/html5/thumbnails/19.jpg)
Tube laser printing - Experimental setup
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Direct-writing height = 1 mm
ArF laser
Pulsed laser beam
Ribbon
Forming jet/droplet
Quartz support
Coating
Container
Optical table
Laser-induced vapor/plasma
3-axis stages
Moving platform
![Page 20: Yong Huang Department of Mechanical Engineering Clemson University, Clemson, SC Scaffold-free Alginate Tube Fabrication using Inkjet and Laser printing](https://reader036.vdocument.in/reader036/viewer/2022062314/56649e4e5503460f94b45807/html5/thumbnails/20.jpg)
Tube laser printing - results
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Top viewSide view
Φ=3 mm
![Page 21: Yong Huang Department of Mechanical Engineering Clemson University, Clemson, SC Scaffold-free Alginate Tube Fabrication using Inkjet and Laser printing](https://reader036.vdocument.in/reader036/viewer/2022062314/56649e4e5503460f94b45807/html5/thumbnails/21.jpg)
Table of Contents Introduction Background Objective Research Tasks
Tube Inkjetting Tube Laser Printing
Conclusions Future Work
21
![Page 22: Yong Huang Department of Mechanical Engineering Clemson University, Clemson, SC Scaffold-free Alginate Tube Fabrication using Inkjet and Laser printing](https://reader036.vdocument.in/reader036/viewer/2022062314/56649e4e5503460f94b45807/html5/thumbnails/22.jpg)
Overhang structures can be scaffold-free fabricated
Viable cellular tubes (3T3) can be printed
ConclusionsInkjetting
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Tubes can be scaffold-free fabricated using LIFT-based laser printing technology
Highly viscous materials (alginate) can be laser printed into well-defined tubular structures
Laser printing
![Page 23: Yong Huang Department of Mechanical Engineering Clemson University, Clemson, SC Scaffold-free Alginate Tube Fabrication using Inkjet and Laser printing](https://reader036.vdocument.in/reader036/viewer/2022062314/56649e4e5503460f94b45807/html5/thumbnails/23.jpg)
Table of Contents Introduction Background Objective Research Tasks
Tube Inkjetting Tube Laser Printing
Conclusions Future Work
23
![Page 24: Yong Huang Department of Mechanical Engineering Clemson University, Clemson, SC Scaffold-free Alginate Tube Fabrication using Inkjet and Laser printing](https://reader036.vdocument.in/reader036/viewer/2022062314/56649e4e5503460f94b45807/html5/thumbnails/24.jpg)
Laser printing of 3D cellular tubes
Printing of adipose-derived stem cell tubes
Future WorkFabrication
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Apoptosis/necrosis test, cell proliferation capacity, and phenotype variation
Post-printing fusion of tubular tissue
Mechanical property measurement of fabricated tubes
Post-printing evaluation
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Thanks
and questions?
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